Protein Overview: ILS1

General Information:
Name(s) found:	ILS1 / YBL076C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|SYBYI4 isoleucine-tRNA ligase (EC 6.1.1.5) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6319395\|ref\|NP_009477.1\| Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A; Ils1p [Saccharomyces cerevisiae] [NCBI NR] gi\|536123\|emb\|CAA84898.1\| ILS1 [Saccharomyces cerevisiae] [NCBI NR] gi\|498991\|emb\|CAA56034.1\| E-1072 protein; ILS1 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|3820\|emb\|CAA30733.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|135134\|sp\|P09436.1\|SYIC_YEAST RecName: Full=Isoleucyl-tRNA synthetase, cytoplasmic; AltName: Full=Isoleucine--tRNA ligase; Short=IleRS [NCBI NR] Isoleucyl-tRNA synthetase, cytoplasmic OS=Saccharomyces cerevisiae GN=ILS1 PE=1 SV=1 [Swiss-Prot] ILS1 Isoleucyl-tRNA synthetase [mips] Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A [SGD] ILS1, Chr II from 81038-84256, reverse complement [yeast_orfs2] Chr II from 81038-84256, reverse complement [yeastorf3.fasta] Chr II from 84256-81038, reverse complement [yeastorf5.fasta] (P09436) Isoleucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.5) (Isoleucine--tRNA ligase) (IleRS) [4932.FASTACCERV.txt] ILS1 SGDID:S0000172, Chr II from 84259-81041, reverse complement, Verified ORF [yeastflysequest.fasta] Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A [Saccharomyces cerevisiae]gi\|498991\|emb\|CAA56034.1\| E-1072 protein; ILS1 protein [Saccharomyces cerevisiae]gi\|536123\|emb\|CAA84898.1\| ILS1 [Saccharomyces cerevisiae [nrdb_11152004_Chlamydomonas] ILS1 SGDID:S000000172, Chr II from 84259-81041, reverse complement, Verified ORF, "Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ILS1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A; Ils1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] E-1072 protein; ILS1 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase) (IleRS) [nr-271106-contam.fasta] [chemostatdb.fasta] Isoleucine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ILS1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ILS1 SGDID:S000000172, Chr II from 84261-81043, Genome Release 64-1-1, reverse complement, Verified ORF, "Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A" [yeast-030211-contam.fasta] ILS1 SGDID:S000000172, Chr II from 84261-81043, Genome Release 64-2-1, reverse complement, Verified ORF, "Cytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A" [20141208_orf_trans.fasta] TR4466\|c0_g1_i1\|g.3159 ORF TR4466\|c0_g1_i1\|g.3159 TR4466\|c0_g1_i1\|m.3159 type:complete len:1073 (+) TR4466\|c0_g1_i1:48-3266(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR4173\|c0_g1_i1\|g.2786 ORF TR4173\|c0_g1_i1\|g.2786 TR4173\|c0_g1_i1\|m.2786 type:complete len:1073 (+) TR4173\|c0_g1_i1:64-3282(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YBL076C SGDID:S000000172, Chr II from 84261-81043, Genome Release 64-1-1, reverse complement, Verified ORF, "Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_4891\|g.3357 ORF asmbl_4891\|g.3357 asmbl_4891\|m.3357 type:complete len:1073 (+) asmbl_4891:67-3285(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_4560\|g.2845 ORF ID=asmbl_4560\|g.2845 ID=asmbl_4560\|m.2845 type:complete len:1073 (+) ID=asmbl_4560:67-3285(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_4889\|g.2788 ORF ID=asmbl_4889\|g.2788 ID=asmbl_4889\|m.2788 type:complete len:1073 (+) ID=asmbl_4889:57-3275(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1072 amino acids
Gene Ontology:
Cellular Component:	cytosol [IDA]
Biological Process:	translation [TAS]
Molecular Function:	isoleucine-tRNA ligase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSESNAHFSF PKEEEKVLSL WDEIDAFHTS LELTKDKPEF SFFDGPPFAT GTPHYGHILA  60
   61 STIKDIVPRY ATMTGHHVER RFGWDTHGVP IEHIIDKKLG ITGKDDVFKY GLENYNNECR 120
  121 SIVMTYASDW RKTIGRLGRW IDFDNDYKTM YPSFMESTWW AFKQLHEKGQ VYRGFKVMPY 180
  181 STGLTTPLSN FEAQQNYKDV NDPAVTIGFN VIGQEKTQLV AWTTTPWTLP SNLSLCVNAD 240
  241 FEYVKIYDET RDRYFILLES LIKTLYKKPK NEKYKIVEKI KGSDLVGLKY EPLFPYFAEQ 300
  301 FHETAFRVIS DDYVTSDSGT GIVHNAPAFG EEDNAACLKN GVISEDSVLP NAIDDLGRFT 360
  361 KDVPDFEGVY VKDADKLIIK YLTNTGNLLL ASQIRHSYPF CWRSDTPLLY RSVPAWFVRV 420
  421 KNIVPQMLDS VMKSHWVPNT IKEKRFANWI ANARDWNVSR NRYWGTPIPL WVSDDFEEVV 480
  481 CVGSIKELEE LTGVRNITDL HRDVIDKLTI PSKQGKGDLK RIEEVFDCWF ESGSMPYASQ 540
  541 HYPFENTEKF DERVPANFIS EGLDQTRGWF YTLAVLGTHL FGSVPYKNVI VSGIVLAADG 600
  601 RKMSKSLKNY PDPSIVLNKY GADALRLYLI NSPVLKAESL KFKEEGVKEV VSKVLLPWWN 660
  661 SFKFLDGQIA LLKKMSNIDF QYDDSVKSDN VMDRWILASM QSLVQFIHEE MGQYKLYTVV 720
  721 PKLLNFIDEL TNWYIRFNRR RLKGENGVED CLKALNSLFD ALFTFVRAMA PFTPFLSESI 780
  781 YLRLKEYIPE AVLAKYGKDG RSVHFLSYPV VKKEYFDEAI ETAVSRMQSV IDLGRNIREK 840
  841 KTISLKTPLK TLVILHSDES YLKDVEALKN YIIEELNVRD VVITSDEAKY GVEYKAVADW 900
  901 PVLGKKLKKD AKKVKDALPS VTSEQVREYL ESGKLEVAGI ELVKGDLNAI RGLPESAVQA 960
  961 GQETRTDQDV LIIMDTNIYS ELKSEGLARE LVNRIQKLRK KCGLEATDDV LVEYELVKDT1020
 1021 IDFEAIVKEH FDMLSKTCRS DIAKYDGSKT DPIGDEEQSI NDTIFKLKVF KL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		DED81 ILS1
View Details	Krogan NJ, et al. (2006)		FRS1 FRS2 ILS1
View Details	Gavin AC, et al. (2006)		DED81 GET3 ILS1
View Details	Gavin AC, et al. (2002)		ADH1 GET3 ILS1 NIP1 UBP14
View Details	Ho Y, et al. (2002)		AAT2 ACC1 ACS2 ADE3 ADE6 ADH4 ADO1 AHA1 ALA1 APE2 ARA1 ARG4 ARO4 BAT1 BMS1 BUD13 CAR2 CDC10 CDC53 CDC60 CIC1 CLU1 CMD1 CMK2 CMP2 COF1 CPR1 CTF4 CYS3 DBP10 DED81 DIA2 DPS1 EDE1 ENP1 ERG10 ERG20 FPR3 GCY1 GPH1 GRS1 HCH1 HOM2 HYP2 ILS1 IMD3 IPP1 KRE33 KRI1 KRS1 LSP1 LST4 MCM2 MCM3 MCM5 MDH3 MPC54 MYO2 MYO4 MYO5 NAS6 NIP7 NMD3 NOP12 OLA1 PAB1 PDC5 PFK2 PGM2 PMI40 PML1 PRP6 PST2 RPA135 RPA190 RPC40 RRP12 SAC6 SCP160 SEH1 SHE3 SKP1 SLT2 SMC4 SOD1 SPB1 SRP1 SSF1 SSF2 SSZ1 SUI1 THR4 TIF6 TPS1 TRP5 TRR1 UBA1 URB1 URE2 UTP10 VAS1 VMA4 VPS13 YAK1 YBL104C YDL124W YNK1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	ILS1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..40] [53..80] [131..142] [592..891]	PSI-BLAST	2680.0	Isoleucyl-tRNA synthetase (IleRS)
2	View Details	[41..52] [81..84] [125..130] [143..147] [420..454] [539..591]	PSI-BLAST	2680.0	Isoleucyl-tRNA synthetase (IleRS)
3	View Details	[85..124] [148..174] [415..419] [455..538]	PSI-BLAST	2680.0	Isoleucyl-tRNA synthetase (IleRS)
4	View Details	[175..200] [396..414]	PSI-BLAST	2680.0	Isoleucyl-tRNA synthetase (IleRS)
5	View Details	[201..395]	PSI-BLAST	2680.0	Isoleucyl-tRNA synthetase (IleRS)
6	View Details	[892..1072]	MSA	2.207964	View MSA. No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: ILS1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: