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Protein Overview: ALA1

General Information:
Name(s) found: ALA1 / YOR335C [SGD]
Description(s) found:
Found 27 descriptions.
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  • Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 958 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed articleLink to PubMed article]
    mitochondrion [IDALink to PubMed articleLink to PubMed article]
    Biological Process: alanyl-tRNA aminoacylation [IGILink to PubMed article][IMPLink to PubMed article]
    Molecular Function: alanine-tRNA ligase activity [IGILink to PubMed article][ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTIGDKQKWT ATNVRNTFLD YFKSKEHKFV KSSPVVPFDD PTLLFANAGM NQYKPIFLGT  60
   61 VDPASDFYTL KRAYNSQKCI RAGGKHNDLE DVGKDSYHHT FFEMLGNWSF GDYFKKEAIT 120
  121 YSWTLLTEVY GIPKDRLYVT YFEGDEKLGL EPDTEARELW KNVGVPDDHI LPGNAKDNFW 180
  181 EMGDQGPCGP CSEIHYDRIG GRNAASLVNM DDPDVLEVWN LVFIQFNREQ DGSLKPLPAK 240
  241 HIDTGMGFER LVSVLQDVRS NYDTDVFTPL FERIQEITSV RPYSGNFGEN DKDGIDTAYR 300
  301 VLADHVRTLT FALADGGVPN NEGRGYVLRR ILRRGARYAR KYMNYPIGNF FSTLAPTLIS 360
  361 QVQDIFPELA KDPAFLFEIL DEEEASFAKT LDRGERLFEK YASAASKTES KTLDGKQVWR 420
  421 LYDTYGFPVD LTELMAEEQG LKIDGPGFEK AKQESYEASK RGGKKDQSDL IKLNVHELSE 480
  481 LNDAKVPKTN DEFKYGSANV EGTILKLHDG TNFVDEITEP GKKYGIILDK TCFYAEQGGQ 540
  541 EYDTGKIVID DAAEFNVENV QLYNGFVFHT GSLEEGKLSV GDKIIASFDE LRRFPIKNNH 600
  601 TGTHILNFAL KETLGNDVDQ KGSLVAPEKL RFDFSHKKAV SNEELKKVED ICNEQIKENL 660
  661 QVFYKEIPLD LAKSIDGVRA VFGETYPDPV RVVSVGKPIE ELLANPANEE WTKYSIEFCG 720
  721 GTHVNKTGDI KYFVILEESG IAKGIRRIVA VTGTEAFEAQ RLAEQFAADL DAADKLPFSP 780
  781 IKEKKLKELG VKLGQLSISV ITKNELKQKF NKIEKAVKDE VKSRAKKENK QTLDEVKTFF 840
  841 ETNENAPYLV KFIDISPNAK AITEAINYMK SNDSVKDKSI YLLAGNDPEG RVAHGCYISN 900
  901 AALAKGIDGS ALAKKVSSII GGKAGGKGNV FQGMGDKPAA IKDAVDDLES LFKEKLSI

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Krogan NJ, et al. (2006) ALA1 ARO1 DUG1 GDH1 HIS4 HIS6 HRD1 MET6 OYE2 RAD14 SCP160 SPE3 SPE4 TIM11 URA6 YLF2 YRB2
    View Details Ho Y, et al. (2002) AAT2 ACS2 ADE3 ADE6 ADH4 ADO1 ALA1 APE2 ARA1 ARG4 AXL1 BAT1 CAR2 CDC10 CDC60 CLU1 CPR1 CYS3 DBF20 DED81 DPS1 EGD2 ENP1 ERG10 ERG20 FPR1 GCY1 GPH1 GRS1 HOM2 HOM3 HYP2 IDH2 ILS1 IMD3 KRS1 LSP1 MDH3 MLP2 MOB1 MPC54 NAS6 OLA1 PAB1 PDC5 PFK2 PGM2 PMI40 PRP6 RPB10 SAC6 SCP160 SOD1 SSZ1 SUI1 THR4 THS1 TPS1 TRR1 UBA1 URA1 YDL124W YDR341C

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2 Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data ALA1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..570] Pfam 288.026872 tRNA synthetases class II (A) No confident structure predictions are available.
    2 View Details [571..889] PSI-BLAST 65.69897 Threonyl-tRNA synthetase (ThrRS), C-terminal domain; Threonyl-tRNA synthetase (ThrRS), N-terminal 'additional' domain; Threonyl-tRNA synthetase (ThrRS), second 'additional' domain; Threonyl-tRNA synthetase (ThrRS)
    3 View Details [890..958] MSA 4.182996 View MSA. Confident ab initio structure predictions are available.
    4 View Details [876..958] MSA 8.240996 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • ligase activity, forming aminoacyl-tRNA and related compounds
    		•  7.89468709683036 bayes_pls_golite062009
  • ligase activity, forming carbon-oxygen bonds
    		•  7.89468709683036 bayes_pls_golite062009
  • aminoacyl-tRNA ligase activity
    		•  7.88410036819636 bayes_pls_golite062009
  • ligase activity
    		•  6.92073480193262 bayes_pls_golite062009
  • alanine-tRNA ligase activity
    		•  6.00693881794242 bayes_pls_golite062009
  • phenylalanine-tRNA ligase activity
    		•  2.99105702052936 bayes_pls_golite062009
  • serine-tRNA ligase activity
    		•  2.46505535069103 bayes_pls_golite062009
  • glycine-tRNA ligase activity
    		•  2.29229136935761 bayes_pls_golite062009
  • threonine-tRNA ligase activity
    		•  2.18642354927 bayes_pls_golite062009
  • catalytic activity
    		•  1.99372273395202 bayes_pls_golite062009
  • lysine-tRNA ligase activity
    		•  1.72324031407565 bayes_pls_golite062009
  • histidine-tRNA ligase activity
    		•  1.72215103383322 bayes_pls_golite062009
  • transcription regulator activity
    		•  1.47976803533584 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.30216701059664 bayes_pls_golite062009
  • DNA binding
    		•  1.1862497958336 bayes_pls_golite062009
  • binding
    		•  1.11470390774037 bayes_pls_golite062009
  • asparagine-tRNA ligase activity
    		•  0.921492123574877 bayes_pls_golite062009
  • transcription repressor activity
    		•  0.80781620905336 bayes_pls_golite062009
  • transcription factor activity
    		•  0.566858624145031 bayes_pls_golite062009
  • RNA binding
    		•  0.0221706730298825 bayes_pls_golite062009
  • aspartate-tRNA ligase activity
    		•  0.00572514744734753 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.96

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle