Protein Overview: SKI2

General Information:
Name(s) found:	SKI2 / YLR398C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|8489004\|sp\|P35207.2\|SKI2_YEAST RecName: Full=Antiviral helicase SKI2; AltName: Full=Superkiller protein 2 [NCBI NR] gi\|6323430\|ref\|NP_013502.1\| Putative RNA helicase, involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski3p and Ski8p; required for repressing propagation of dsRNA viruses; Ski2p [Sac [NCBI NR] gi\|625114\|gb\|AAB82356.1\| Ski2p: Antiviral protein [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S55954 viral mRNA translation inhibitor SKI2 - yeast (Saccharomyces cerevisiae) [NCBI NR] Antiviral helicase SKI2 OS=Saccharomyces cerevisiae GN=SKI2 PE=1 SV=2 [Swiss-Prot] SKI2 Antiviral protein and putative helicase [mips] Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay [SGD] SKI2, Chr XII from 915155-919018, reverse complement [yeast_orfs2] Chr XII from 915155-919018, reverse complement [yeastorf3.fasta] Chr XII from 919018-915155, reverse complement [yeastorf5.fasta] (P35207) Antiviral protein SKI2 [4932.FASTACCERV.txt] SKI2 SGDID:S0004390, Chr XII from 919019-915156, reverse complement, Verified ORF [yeastflysequest.fasta] Ski2p [Saccharomyces cerevisiae]gi\|8489004\|sp\|P35207\|SKI2_YEAST Antiviral protein SKI2gi\|1084512\|pir\|\|S55954 viral mRNA translation inhibitor SKI2 - yeast (Saccharomyces cerevisiae)gi\|625114\|gb\|AAB82356.1\| Ski2p: Antiviral protein [Saccharomyces cerevi [nrdb_11152004_Chlamydomonas] SKI2 SGDID:S000004390, Chr XII from 919019-915156, reverse complement, Verified ORF, "Putative RNA helicase, involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski3p and Ski8p; requir [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SKI2 SGDID:S000004390, Chr XII from 919019-915156, reverse complement, Verified ORF, "Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral ds [SGD_S-cerevisiae_na_05-08-2009_con_reversed.fasta] Antiviral helicase SKI2 [nr-271106-contam.fasta] Putative RNA helicase, involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski3p and Ski8p; required for repressing propagation of dsRNA viruses; Ski2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ski2p: Antiviral protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SKI2 SGDID:S000004390, Chr XII from 919019-915156, reverse complement, Verified ORF, "Putative RNA helicase, involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski3p and Ski8p; required for repressing propagation of dsRNA viruses" [yeast-bovrabprots.fasta] SKI2 SGDID:S000004390, Chr XII from 919019-915156, reverse complement, Verified ORF, "Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay" [scerevisihumanrev.fasta] [chemostatdb.fasta] Antiviral helicase SKI2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SKI2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SKI2 SGDID:S000004390, Chr XII from 919019-915156, Genome Release 64-1-1, reverse complement, Verified ORF, "Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay" [yeast-030211-contam.fasta] SKI2 SGDID:S000004390, Chr XII from 919019-915156, Genome Release 64-2-1, reverse complement, Verified ORF, "Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome" [20141208_orf_trans.fasta] TR1101\|c0_g1_i1\|g.861 ORF TR1101\|c0_g1_i1\|g.861 TR1101\|c0_g1_i1\|m.861 type:complete len:1288 (+) TR1101\|c0_g1_i1:115-3978(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YLR398C SGDID:S000004390, Chr XII from 919019-915156, Genome Release 64-1-1, reverse complement, Verified ORF, "Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_7763\|g.5486 ORF asmbl_7763\|g.5486 asmbl_7763\|m.5486 type:complete len:1288 (+) asmbl_7763:41-3904(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_7541\|g.4745 ORF ID=asmbl_7541\|g.4745 ID=asmbl_7541\|m.4745 type:complete len:1288 (+) ID=asmbl_7541:41-3904(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1287 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] nucleolus [TAS]
Biological Process:	regulation of translation [IGI ][IMP ] response to exogenous dsRNA [IMP] mRNA catabolic process [IMP][TAS]
Molecular Function:	RNA helicase activity [ISS] translation repressor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSEGFSSSSI QELYQSLKEI TNNADVELFE DRITKLDFES TDEPKHANDI IKDRFLRPSN  60
   61 ALPWSLLDMV QDVPHTSSPE DCSGKLDYKE LLKVPDPINR TSYQFKRTGL EGKISGYKEE 120
  121 VDLKEVANAN ASNSLSITRS INHNQNSVRG STAQLPFTPG GIPMKSVKTD SEQNGSSTMA 180
  181 NATKLLHKDG QGLFDIPEGM NRGIKPMDSP AENEDQNGQF KELKQLNEID NELDIRIEAN 240
  241 EAKLKEEEKS AKSISEEIME EATEETTADN ADDAEIDELL PIGIDFGRTK PVSKSVPVKK 300
  301 EWAHVVDLNH KIENFDELIP NPARSWPFEL DTFQKEAVYH LEQGDSVFVA AHTSAGKTVV 360
  361 AEYAIAMAHR NMTKTIYTSP IKALSNQKFR DFKETFDDVN IGLITGDVQI NPDANCLIMT 420
  421 TEILRSMLYR GADLIRDVEF VIFDEVHYVN DQDRGVVWEE VIIMLPQHVK FILLSATVPN 480
  481 TYEFANWIGR TKQKNIYVIS TPKRPVPLEI NIWAKKELIP VINQNSEFLE ANFRKHKEIL 540
  541 NGESAKGAPS KTDNGRGGST ARGGRGGSNT RDGRGGRGNS TRGGANRGGS RGAGAIGSNK 600
  601 RKFFTQDGPS KKTWPEIVNY LRKRELLPMV VFVFSKKRCE EYADWLEGIN FCNNKEKSQI 660
  661 HMFIEKSITR LKKEDRDLPQ ILKTRSLLER GIAVHHGGLL PIVKELIEIL FSKGFIKVLF 720
  721 ATETFAMGLN LPTRTVIFSS IRKHDGNGLR ELTPGEFTQM AGRAGRRGLD STGTVIVMAY 780
  781 NSPLSIATFK EVTMGVPTRL QSQFRLTYNM ILNLLRIEAL RVEEMIKYSF SENAKETLQP 840
  841 EHEKQIKVLQ EELQTIEYKS CEICDNDIEK FLELMLAYKE ATVNLMQEMV KSPSILHILK 900
  901 EGRLVAFRDP NDCLKLGFVF KVSLKDAVCV IMTFTKPYKL PNGEPNHLIY FPKADGYRRR 960
  961 NFPKFQKTDF YMEEVPVTAI EVITKRKFAA PLGKVIKKDV AALNEFNAET NNILDGKTLK1020
 1021 EAINIEKQGL KIHQILLDRT NIRDEIFKLK SIKCPNLSQH IVPKFKAHVI KKKIEELYHL1080
 1081 MSDQNLSLLP DYEKRLAVLK DTEFIDQNHN VLLKGRVACE INSGYELVLT ELILDNFLGS1140
 1141 FEPEEIVALL SVFVYEGKTR EEEPPIVTPR LAKGKQRIEE IYKKMLCVFN THQIPLTQDE1200
 1201 AEFLDRKRFA MMNVVYEWAR GLSFKEIMEM SPEAEGTVVR VITWLDEICR EVKTASIIIG1260
 1261 NSTLHMKMSR AQELIKRDIV FAASLYL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		FIT1 RRP43 SKI2 SKI3 SKI8 YKL023W
View Details	Krogan NJ, et al. (2006)		ENT5 SKI2 SKI3 SKI8 YKL023W
View Details	Gavin AC, et al. (2006)		SKI2 SKI3 SKI8
View Details	Gavin AC, et al. (2002)		ARX1 BEM2 BMS1 BRX1 BUD20 CIC1 CLU1 CSL4 CYR1 CYS4 DBP10 DBP9 DCP2 DIS3 DRS1 EBP2 ECM16 ERB1 FPR4 GCN1 HAS1 HDA1 HER1 HIS3 INP2 IPI1 IPI3 KRE33 LHP1 LOC1 LRP1 LSG1 MAK21 MAK5 MDN1 MIS1 MRT4 MTR3 NAP1 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP7 NSA1 NSA2 NUG1 PRT1 PUF6 REI1 RIX1 RIX7 RLP24 RLP7 RPA135 RPA190 RPF1 RPF2 RPG1 RPP2B RPT6 RRP1 RRP12 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 RSA3 RSA4 RTR1 SDA1 SEC7 SKI2 SKI3 SKI6 SKI7 SNU13 SPB1 SPB4 SRP1 SSF1 TIF6 TUB3 URB1 UTP10 UTP20 UTP22 YGL036W YIR035C YTM1
View Details	Ho Y, et al. (2002)		AKL1 SKI2 SKI3 SKI8
View Details	Qiu et al. (2008)		SKI2 SKI8

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NSL1	No Comments	Hazbun TR, et al. (2003)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	SKI2	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..170]	MSA	1.118936	View MSA. No confident structure predictions are available.
2	View Details	[171..274]	deduced	N/A	No confident structure predictions are available.
3	View Details	[275..667]	PSI-BLAST	96.522879	Initiation factor 4a
4	View Details	[668..891]	PSI-BLAST	7.0	Nucleotide excision repair enzyme UvrB
5	View Details	[892..964]	deduced	N/A	Confident ab initio structure predictions are available.
6	View Details	[965..1287]	ab initio	7.51	No description for 1n1bA was found.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: SKI2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: