Protein Overview: YEF3

General Information:
Name(s) found:	YEF3 / YLR249W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|662335\|gb\|AAB67391.1\| Yef3p: Elongation factor 3 (EF-3) [Saccharomyces cerevisiae] [NCBI NR] gi\|6323278\|ref\|NP_013350.1\| Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP; Yef3p [Saccharomyces c [NCBI NR] pir\|\|DVBYE3 translation elongation factor eEF-3 - yeast (Saccharomyces cerevisiae) [NCBI NR] YEF3 Translation elongation factor eEF3 [mips] Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP [SGD] YEF3, Chr XII from 636780-639914 [yeast_orfs2] Chr XII from 636780-639914 [yeastorf3.fasta] YEF3 SGDID:S0004239, Chr XII from 636782-639916, Verified ORF [yeastflysequest.fasta] Yef3p [Saccharomyces cerevisiae]gi\|2144924\|pir\|\|DVBYE3 translation elongation factor eEF-3 - yeast (Saccharomyces cerevisiae)gi\|662335\|gb\|AAB67391.1\| Yef3p: Elongation factor 3 (EF-3) [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] YEF3 SGDID:S000004239, Chr XII from 636782-639916, Verified ORF, "Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hy [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YEF3 SGDID:S000004239, Chr XII from 636782-639916, Verified ORF, "Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Yef3p: Elongation factor 3 (EF-3) [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP; Yef3p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YEF3 SGDID:S000004239, Chr XII from 636782-639916, Verified ORF, "Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP" [yeast-bovrabprots.fasta] YEF3 SGDID:S000004239, Chr XII from 636782-639916, Verified ORF, "Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP" [yeast-contam_custom.fasta] [chemostatdb.fasta] Elongation factor 3A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YEF3 PE=1 SV=4 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] YEF3 SGDID:S000004239, Chr XII from 636780-639914, Genome Release 64-1-1, Verified ORF, "Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP" [yeast-030211-contam.fasta] YEF3 SGDID:S000004239, Chr XII from 636780-639914, Genome Release 64-2-1, Verified ORF, "Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR1980\|c0_g1_i1\|g.1460 ORF TR1980\|c0_g1_i1\|g.1460 TR1980\|c0_g1_i1\|m.1460 type:complete len:1045 (+) TR1980\|c0_g1_i1:52-3186(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YLR249W SGDID:S000004239, Chr XII from 636780-639914, Genome Release 64-1-1, Verified ORF, "Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_7659\|g.4605 ORF ID=asmbl_7659\|g.4605 ID=asmbl_7659\|m.4605 type:complete len:1045 (+) ID=asmbl_7659:41-3175(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1044 amino acids
Gene Ontology:
Cellular Component:	ribosome [IDA]
Biological Process:	ribosome biogenesis [RCA] translational elongation [TAS]
Molecular Function:	translation elongation factor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDSQQSIKV LEELFQKLSV ATADNRHEIA SEVASFLNGN IIEHDVPEHF FGELAKGIKD  60
   61 KKTAANAMQA VAHIANQSNL SPSVEPYIVQ LVPAICTNAG NKDKEIQSVA SETLISIVNA 120
  121 VNPVAIKALL PHLTNAIVET NKWQEKIAIL AAISAMVDAA KDQVALRMPE LIPVLSETMW 180
  181 DTKKEVKAAA TAAMTKATET VDNKDIERFI PSLIQCIADP TEVPETVHLL GATTFVAEVT 240
  241 PATLSIMVPL LSRGLNERET GIKRKSAVII DNMCKLVEDP QVIAPFLGKL LPGLKSNFAT 300
  301 IADPEAREVT LRALKTLRRV GNVGEDDAIP EVSHAGDVST TLQVVNELLK DETVAPRFKI 360
  361 VVEYIAAIGA DLIDERIIDQ QAWFTHITPY MTIFLHEKKA KDILDEFRKR AVDNIPVGPN 420
  421 FDDEEDEGED LCNCEFSLAY GAKILLNKTQ LRLKRARRYG ICGPNGCGKS TLMRAIANGQ 480
  481 VDGFPTQEEC RTVYVEHDID GTHSDTSVLD FVFESGVGTK EAIKDKLIEF GFTDEMIAMP 540
  541 ISALSGGWKM KLALARAVLR NADILLLDEP TNHLDTVNVA WLVNYLNTCG ITSITISHDS 600
  601 VFLDNVCEYI INYEGLKLRK YKGNFTEFVK KCPAAKAYEE LSNTDLEFKF PEPGYLEGVK 660
  661 TKQKAIVKVT NMEFQYPGTS KPQITDINFQ CSLSSRIAVI GPNGAGKSTL INVLTGELLP 720
  721 TSGEVYTHEN CRIAYIKQHA FAHIESHLDK TPSEYIQWRF QTGEDRETMD RANRQINEND 780
  781 AEAMNKIFKI EGTPRRIAGI HSRRKFKNTY EYECSFLLGE NIGMKSERWV PMMSVDNAWI 840
  841 PRGELVESHS KMVAEVDMKE ALASGQFRPL TRKEIEEHCS MLGLDPEIVS HSRIRGLSGG 900
  901 QKVKLVLAAG TWQRPHLIVL DEPTNYLDRD SLGALSKALK EFEGGVIIIT HSAEFTKNLT 960
  961 EEVWAVKDGR MTPSGHNWVS GQGAGPRIEK KEDEEDKFDA MGNKIAGGKK KKKLSSAELR1020
 1021 KKKKERMKKK KELGDAYVSS DEEF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ABP1 ADH1 ADH3 CAM1 EFB1 EFT2, EFT1 FBA1 GUS1 HSC82 HSP104 HSP82 HXK1 HYP2 PDC1 PIL1 RPG1 RPL40B, RPL40A SSZ1 TDH2 TEF4 TIF1, TIF2 YEF3 ZUO1
View Details	Krogan NJ, et al. (2006)		HFM1 LYS4 NEW1 RBG1 SGD1 SOK1 SSZ1 TCB1 TGL5 TMA46 YEF3 YLR072W ZUO1
View Details	Gavin AC, et al. (2002)		ACT1 ADH1 AOS1 ARX1 BGL2 BRR1 CBC2 CDC14 CDC33 CLF1 CSN12 CUS1 CWC2 DIB1 ECM2 GCN1 GIN4 HMO1 HSC82 HSH155 HSP42 ISW1 KAP114 KAP123 KCC4 LEA1 LSM4 LUC7 MLC1 MLC2 MOT1 MSL1 MUD1 MYO1 MYO2 MYO4 NAM8 NET1 NIP1 NPL3 PBP2 PDC1 PFK2 PGI1 PRP11 PRP19 PRP21 PRP3 PRP31 PRP39 PRP4 PRP40 PRP42 PRP43 PRP46 PRP6 PRP9 RAD1 RAD10 RPN9 RPT5 RSE1 SHE2 SHE3 SIR2 SIR4 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU56 SNU66 SNU71 SPP382 SPT15 SRB2 STO1 TAF1 TAF5 TAF6 TDH3 TIF4631 TIF4632 UBA2 VPS1 YEF3 YRA1
View Details	Qiu et al. (2008)		TEF1, TEF2 YEF3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	PSF2	No Comments	Hazbun TR, et al. (2003)
View Run	UTP23	No Comments	Hazbun TR, et al. (2003)
View Run	PGA1	No Comments	Hazbun TR, et al. (2003)
View Run	PGA1	No Comments	Hazbun TR, et al. (2003)
View Run	MIA40	No Comments	Hazbun TR, et al. (2003)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ASF1	sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant)	Green EM, et al (2005)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#34 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	YEF3	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..71]	ORFEUS	13.93	Lipovitellin-phosvitin complex, superhelical domain; Lipovitellin-phosvitin complex; beta-sheet shell regions
2	View Details	[72..179]	ORFEUS	13.93	Lipovitellin-phosvitin complex, superhelical domain; Lipovitellin-phosvitin complex; beta-sheet shell regions
3	View Details	[180..324]	ORFEUS	13.93	Lipovitellin-phosvitin complex, superhelical domain; Lipovitellin-phosvitin complex; beta-sheet shell regions
4	View Details	[325..424]	deduced	N/A	No confident structure predictions are available.
5	View Details	[425..662]	PSI-BLAST	105.0	Maltose transport protein MalK, C-terminal domain; Maltose transport protein MalK, N-terminal domain
6	View Details	[663..686] [707..806]	PSI-BLAST	4.69897	Smc head domain
7	View Details	[687..706] [807..975]	PSI-BLAST	4.69897	Smc head domain
8	View Details	[976..1044]	deduced	N/A	No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: YEF3

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: