Protein Overview: ARF1

General Information:
Name(s) found:	ARF1 / YDL192W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320009\|ref\|NP_010089.1\| ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p; Arf1p [Saccharomyces cerevisiae] [NCBI NR] gi\|171073\|gb\|AAA34431.1\| ADP-ribosylation factor [NCBI NR] gi\|1431314\|emb\|CAA98769.1\| ARF1 [Saccharomyces cerevisiae] [NCBI NR] gi\|114121\|sp\|P11076.3\|ARF1_YEAST RecName: Full=ADP-ribosylation factor 1 [NCBI NR] pir\|\|B36167 ADP-ribosylation factor 1 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1004303\|emb\|CAA58255.1\| ADP-ribosylationfactor 2 [Saccharomyces cerevisiae] [NCBI NR] ADP-ribosylation factor 1 OS=Saccharomyces cerevisiae GN=ARF1 PE=1 SV=3 [Swiss-Prot] ARF1 Small GTP-binding protein of the ARF family [mips] ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p [SGD] ARF1, Chr IV from 116322-116867 [yeast_orfs2] Chr IV from 116322-116867 [yeastorf3.fasta] ARF1 SGDID:S0002351, Chr IV from 116322-116867, Verified ORF [yeastflysequest.fasta] Arf1p [Saccharomyces cerevisiae]gi\|1431314\|emb\|CAA98769.1\| ARF1 [Saccharomyces cerevisiae]gi\|1004303\|emb\|CAA58255.1\| ADP-ribosylationfactor 2 [Saccharomyces cerevisiae]gi\|101185\|pir\|\|B36167 ADP-ribosylation factor 1 - yeast (Saccharomyces cerevisiae)g [nrdb_11152004_Chlamydomonas] ARF1 SGDID:S000002351, Chr IV from 116322-116867, Verified ORF, "ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Ar [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ARF1 SGDID:S000002351, Chr IV from 116322-116867, Verified ORF, "ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; functionally interchangeable with Arf [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] ADP-ribosylation factor [nr-271106-contam.fasta] ADP-ribosylation factor 1 [nr-271106-contam.fasta] ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p; Arf1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ADP-ribosylationfactor 2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ARF1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ARF1 SGDID:S000002351, Chr IV from 116322-116867, Verified ORF, "ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p" [yeast-bovrabprots.fasta] ARF1 SGDID:S000002351, Chr IV from 116322-116867, Verified ORF, "ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p" [yeast-contam_custom.fasta] [chemostatdb.fasta] ADP-ribosylation factor 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ARF1 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ARF1 SGDID:S000002351, Chr IV from 116321-116866, Genome Release 64-1-1, Verified ORF, "ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p" [yeast-030211-contam.fasta] ARF1 SGDID:S000002351, Chr IV from 116321-116866, Genome Release 64-2-1, Verified ORF, "ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR5448\|c0_g2_i1\|g.3861 ORF TR5448\|c0_g2_i1\|g.3861 TR5448\|c0_g2_i1\|m.3861 type:complete len:182 (+) TR5448\|c0_g2_i1:131-676(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YDL192W SGDID:S000002351, Chr IV from 116321-116866, Genome Release 64-1-1, Verified ORF, "ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; functionally interchangeable with Arf2p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_536\|g.199 ORF asmbl_536\|g.199 asmbl_536\|m.199 type:complete len:182 (+) asmbl_536:116-661(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_537\|g.740 ORF ID=asmbl_537\|g.740 ID=asmbl_537\|m.740 type:complete len:182 (+) ID=asmbl_537:116-661(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_649\|g.54 ORF ID=asmbl_649\|g.54 ID=asmbl_649\|m.54 type:complete len:182 (+) ID=asmbl_649:132-677(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	181 amino acids
Gene Ontology:
Cellular Component:	cytosol [TAS ] Golgi-associated vesicle [TAS ]
Biological Process:	ER to Golgi vesicle-mediated transport [TAS ] Golgi to plasma membrane transport [IGI][IPI] telomere maintenance [IMP] intra-Golgi vesicle-mediated transport [TAS ]
Molecular Function:	GTPase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGLFASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG FNVETVQYKN  60
   61 ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VVDSNDRSRI GEAREVMQRM LNEDELRNAA 120
  121 WLVFANKQDL PEAMSAAEIT EKLGLHSIRN RPWFIQATCA TSGEGLYEGL EWLSNSLKNS 180
  181 T

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ARF1 ARF2
View Details	(MIPS) Mewes HW, et al. (2004)		ARF1 COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
View Details	Ho Y, et al. (2002)		AAT2 ACH1 ACO1 AFR1 AHA1 AHP1 ARC1 ARF1 ARF2 ATP3 CAR2 CDC33 CLU1 COF1 COR1 CPR1 CTF13 CYR1 CYS4 EGD1 ERG13 ERG6 FPR1 FRS2 GAL83 GIS4 GND1 GND2 GPG1 GPI16 GRX1 GUS1 HEF3 HHF1, HHF2 HTB1 KRI1 LHS1 LRO1 LSP1 MET6 MGE1 MGT1 NTF2 OLA1 OYE2 PFK1 PIL1 PRB1 PRM2 RIP1 RNR2 RPA135 RPA190 RPC19 RPC40 RSN1 SAH1 SCP160 SEC27 SEC53 SEN15 SES1 SKP1 SNF1 SNF4 SNU13 SOD1 SOF1 TEF4 THS1 TIF1, TIF2 TMA19 TSA2 UBA1 UBI4 UBR1 VMA4 VMA5 WTM1 YHR033W YNK1
View Details	Qiu et al. (2008)		ARF1 ARF2 COP1 DRS2 ERV25 RET2 RET3 SAR1 SEC13 SEC21 SEC26 SEC27 SEC28 VRG4 YOS1 YPT31

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TIM50	No Comments	Hazbun TR, et al. (2003)
View Run	MIA40	No Comments	Hazbun TR, et al. (2003)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ARF1	PBN1	1	Unpublished Fields Lab Data
View Screen	ARF1	BUD3	1	Unpublished Fields Lab Data
View Screen	ARF1	SRO9	1	Unpublished Fields Lab Data
View Screen	ARF1	TPO1	1	Unpublished Fields Lab Data
View Screen	ARF1	YER159C-A, YDR31...	1	Unpublished Fields Lab Data
View Screen	ARF1	PIS1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	ARF1	Huh WK, et al. (2003)
View Data	ARF1 and CHC1	Huh WK, et al. (2003)
View Data	ARF1 and COP1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..181]	PSI-BLAST	869.0103	ADP-ribosylation factor

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
microtubule motor activity	4.51968578934603	bayes_pls_golite062009
motor activity	4.04927796049806	bayes_pls_golite062009
transporter activity	3.10463051760206	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.95079405147356	bayes_pls_golite062009
pyrophosphatase activity	2.87427509025394	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.85499564954033	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.85178667290149	bayes_pls_golite062009
structural constituent of muscle	2.82808660259595	bayes_pls_golite062009
tubulin binding	2.67743463093593	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
primary active transmembrane transporter activity	2.61775212528604	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.61467977505707	bayes_pls_golite062009
microtubule binding	2.59155206524122	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.57552435627908	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.57107347424517	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.57054427875021	bayes_pls_golite062009
NAD(P)+-protein-arginine ADP-ribosyltransferase activity	2.56769246638533	bayes_pls_golite062009
microfilament motor activity	2.51786963027655	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
ATPase activity, coupled	2.43862163407966	bayes_pls_golite062009
cytoskeletal protein binding	2.4101693596493	bayes_pls_golite062009
ATPase activity	2.39535714892224	bayes_pls_golite062009
actin binding	2.2973522188675	bayes_pls_golite062009
amine transmembrane transporter activity	2.29486577878686	bayes_pls_golite062009
nucleic acid binding	2.09956015274464	bayes_pls_golite062009
transcription regulator activity	2.09345198747025	bayes_pls_golite062009
substrate-specific transporter activity	1.91091452720653	bayes_pls_golite062009
guanyl nucleotide binding	1.89320355239381	bayes_pls_golite062009
guanyl ribonucleotide binding	1.88721318481223	bayes_pls_golite062009
GTP binding	1.87900088157196	bayes_pls_golite062009
DNA binding	1.87765278833074	bayes_pls_golite062009
alpha-tubulin binding	1.792591628112	bayes_pls_golite062009
organic acid transmembrane transporter activity	1.77111739879769	bayes_pls_golite062009
carboxylic acid transmembrane transporter activity	1.75695995185278	bayes_pls_golite062009
protein transporter activity	1.71214667007262	bayes_pls_golite062009
RNA helicase activity	1.63960194106079	bayes_pls_golite062009
GTPase activity	1.6309422514633	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.61735400124552	bayes_pls_golite062009
RNA-dependent ATPase activity	1.60404584546501	bayes_pls_golite062009
hydrolase activity	1.57549510726226	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.56325751172863	bayes_pls_golite062009
signal recognition particle binding	1.46975774710831	bayes_pls_golite062009
plus-end-directed microtubule motor activity	1.38366133153678	bayes_pls_golite062009
microtubule plus-end binding	1.36713729043205	bayes_pls_golite062009
actin filament binding	1.28204719195916	bayes_pls_golite062009
protein binding	1.22129154759482	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
ATP binding	0.98146619596929	bayes_pls_golite062009
ion transmembrane transporter activity	0.977237423302079	bayes_pls_golite062009
adenyl ribonucleotide binding	0.949788783884952	bayes_pls_golite062009
adenyl nucleotide binding	0.931486373329219	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
actin-dependent ATPase activity	0.92105578610354	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
kinase activity	0.691112783105676	bayes_pls_golite062009
molecular transducer activity	0.683309012097319	bayes_pls_golite062009
signal transducer activity	0.683309012097319	bayes_pls_golite062009
transcription factor activity	0.682439846757997	bayes_pls_golite062009
transmembrane receptor activity	0.661834961742057	bayes_pls_golite062009
beta-tubulin binding	0.654735466720932	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.652333406433794	bayes_pls_golite062009
receptor activity	0.633514002718998	bayes_pls_golite062009
DNA strand annealing activity	0.631634545479169	bayes_pls_golite062009
helicase activity	0.6191010101321	bayes_pls_golite062009
amino acid transmembrane transporter activity	0.585668369219566	bayes_pls_golite062009
cation transmembrane transporter activity	0.557919489256916	bayes_pls_golite062009
ribonucleoprotein binding	0.53788302029845	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.499834566483656	bayes_pls_golite062009
microtubule-severing ATPase activity	0.491769258942331	bayes_pls_golite062009
drug transmembrane transporter activity	0.38711726712063	bayes_pls_golite062009
transition metal ion transmembrane transporter activity	0.305627755818843	bayes_pls_golite062009
ATP-dependent helicase activity	0.29664013896754	bayes_pls_golite062009
purine NTP-dependent helicase activity	0.29664013896754	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.213018989352421	bayes_pls_golite062009
structural constituent of ribosome	0.208665086324262	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.123304946374679	bayes_pls_golite062009
cell surface binding	0.0454774504404609	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.0430764033739892	bayes_pls_golite062009
protein kinase activity	0.0364322017534667	bayes_pls_golite062009
structural constituent of cytoskeleton	0.0111338193708552	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2