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Protein Overview: PRP8

General Information:
Name(s) found: PRP8 / YHR165C [SGD]
Description(s) found:
Found 26 descriptions.
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  • Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing; mutations of human Prp8 cause retinitis pigmentosa [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 2413 amino acids

    Gene Ontology:
    Cellular Component: U4/U6 x U5 tri-snRNP complex [IDALink to PubMed article][IPILink to PubMed article]
    U5 snRNP [IDALink to PubMed article][IPILink to PubMed articleLink to PubMed article]
    Biological Process: nuclear mRNA 3'-splice site recognition [IMPLink to PubMed article]
    nuclear mRNA splicing, via spliceosome [IPILink to PubMed article]
    Molecular Function: U5 snRNA binding [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSGLPPPPPG FEEDSDLALP PPPPPPPGYE IEELDNPMVP SSVNEDTFLP PPPPPPSNFE  60
   61 INAEEIVDFT LPPPPPPPGL DELETKAEKK VELHGKRKLD IGKDTFVTRK SRKRAKKMTK 120
  121 KAKRSNLYTP KAEMPPEHLR KIINTHSDMA SKMYNTDKKA FLGALKYLPH AILKLLENMP 180
  181 HPWEQAKEVK VLYHTSGAIT FVNETPRVIE PVYTAQWSAT WIAMRREKRD RTHFKRMRFP 240
  241 PFDDDEPPLS YEQHIENIEP LDPINLPLDS QDDEYVKDWL YDSRPLEEDS KKVNGTSYKK 300
  301 WSFDLPEMSN LYRLSTPLRD EVTDKNYYYL FDKKSFFNGK ALNNAIPGGP KFEPLYPREE 360
  361 EEDYNEFNSI DRVIFRVPIR SEYKVAFPHL YNSRPRSVRI PWYNNPVSCI IQNDEEYDTP 420
  421 ALFFDPSLNP IPHFIDNNSS LNVSNTKENG DFTLPEDFAP LLAEEEELIL PNTKDAMSLY 480
  481 HSPFPFNRTK GKMVRAQDVA LAKKWFLQHP DEEYPVKVKV SYQKLLKNYV LNELHPTLPT 540
  541 NHNKTKLLKS LKNTKYFQQT TIDWVEAGLQ LCRQGHNMLN LLIHRKGLTY LHLDYNFNLK 600
  601 PTKTLTTKER KKSRLGNSFH LMRELLKMMK LIVDTHVQFR LGNVDAFQLA DGIHYILNHI 660
  661 GQLTGIYRYK YKVMHQIRAC KDLKHIIYYK FNKNLGKGPG CGFWQPAWRV WLNFLRGTIP 720
  721 LLERYIGNLI TRQFEGRSNE IVKTTTKQRL DAYYDLELRN SVMDDILEMM PESIRQKKAR 780
  781 TILQHLSEAW RCWKANIPWD VPGMPAPIKK IIERYIKSKA DAWVSAAHYN RERIKRGAHV 840
  841 EKTMVKKNLG RLTRLWIKNE QERQRQIQKN GPEITPEEAT TIFSVMVEWL ESRSFSPIPF 900
  901 PPLTYKNDTK ILVLALEDLK DVYASKVRLN ASEREELALI EEAYDNPHDT LNRIKKYLLT 960
  961 QRVFKPVDIT MMENYQNISP VYSVDPLEKI TDAYLDQYLW YEADQRKLFP NWIKPSDSEI1020
 1021 PPLLVYKWTQ GINNLSEIWD VSRGQSAVLL ETTLGEMAEK IDFTLLNRLL RLIVDPNIAD1080
 1081 YITAKNNVVI NFKDMSHVNK YGLIRGLKFA SFIFQYYGLV IDLLLLGQER ATDLAGPANN1140
 1141 PNEFMQFKSK EVEKAHPIRL YTRYLDRIYM LFHFEEDEGE ELTDEYLAEN PDPNFENSIG1200
 1201 YNNRKCWPKD SRMRLIRQDV NLGRAVFWEI QSRVPTSLTS IKWENAFVSV YSKNNPNLLF1260
 1261 SMCGFEVRIL PRQRMEEVVS NDEGVWDLVD ERTKQRTAKA YLKVSEEEIK KFDSRIRGIL1320
 1321 MASGSTTFTK VAAKWNTSLI SLFTYFREAI VATEPLLDIL VKGETRIQNR VKLGLNSKMP1380
 1381 TRFPPAVFYT PKELGGLGMI SASHILIPAS DLSWSKQTDT GITHFRAGMT HEDEKLIPTI1440
 1441 FRYITTWENE FLDSQRVWAE YATKRQEAIQ QNRRLAFEEL EGSWDRGIPR ISTLFQRDRH1500
 1501 TLAYDRGHRI RREFKQYSLE RNSPFWWTNS HHDGKLWNLN AYRTDVIQAL GGIETILEHT1560
 1561 LFKGTGFNSW EGLFWEKASG FEDSMQFKKL THAQRTGLSQ IPNRRFTLWW SPTINRANVY1620
 1621 VGFLVQLDLT GIFLHGKIPT LKISLIQIFR AHLWQKIHES IVFDICQILD GELDVLQIES1680
 1681 VTKETVHPRK SYKMNSSAAD ITMESVHEWE VSKPSLLHET NDSFKGLITN KMWFDVQLRY1740
 1741 GDYDSHDISR YVRAKFLDYT TDNVSMYPSP TGVMIGIDLA YNMYDAYGNW FNGLKPLIQN1800
 1801 SMRTIMKANP ALYVLRERIR KGLQIYQSSV QEPFLNSSNY AELFNNDIKL FVDDTNVYRV1860
 1861 TVHKTFEGNV ATKAINGCIF TLNPKTGHLF LKIIHTSVWA GQKRLSQLAK WKTAEEVSAL1920
 1921 VRSLPKEEQP KQIIVTRKAM LDPLEVHMLD FPNIAIRPTE LRLPFSAAMS IDKLSDVVMK1980
 1981 ATEPQMVLFN IYDDWLDRIS SYTAFSRLTL LLRALKTNEE SAKMILLSDP TITIKSYHLW2040
 2041 PSFTDEQWIT IESQMRDLIL TEYGRKYNVN ISALTQTEIK DIILGQNIKA PSVKRQKMAE2100
 2101 LEAARSEKQN DEEAAGASTV MKTKTINAQG EEIVVVASAD YESQTFSSKN EWRKSAIANT2160
 2161 LLYLRLKNIY VSADDFVEEQ NVYVLPKNLL KKFIEISDVK IQVAAFIYGM SAKDHPKVKE2220
 2221 IKTVVLVPQL GHVGSVQISN IPDIGDLPDT EGLELLGWIH TQTEELKFMA ASEVATHSKL2280
 2281 FADKKRDCID ISIFSTPGSV SLSAYNLTDE GYQWGEENKD IMNVLSEGFE PTFSTHAQLL2340
 2341 LSDRITGNFI IPSGNVWNYT FMGTAFNQEG DYNFKYGIPL EFYNEMHRPV HFLQFSELAG2400
 2401 DEELEAEQID VFS

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) AAR2 BRR1 BRR2 CEF1 CLF1 CUS1 CWC2 CWC22 CWC23 DIB1 HSH155 KEM1 LEA1 LIN1 LSM1 LSM2 LSM4 LSM5 LSM6 LSM7 LSM8 LUC7 PAT1 PRP11 PRP19 PRP21 PRP3 PRP31 PRP38 PRP4 PRP40 PRP43 PRP45 PRP46 PRP6 PRP8 PRP9 RSE1 SMB1 SMD1 SMD2 SMD3 SME1 SMX3 SNP1 SNT309 SNU114 SNU23 SNU66 SNU71 SPP382 STO1 SYF1
    View Details Hazbun TR, et al. (2003) BRR2 CDC40 CEF1 CLF1 CWC2 CWC22 CWC23 CWC25 ECM2 ISY1 LEA1 NTR2 PRP19 PRP43 PRP45 PRP46 PRP8 SMB1 SMD1 SMD3 SMX3 SNT309 SNU114 SPP382 SYF1 SYF2 YJU2
    View Details (MIPS) Mewes HW, et al. (2004) AAR2 BRR1 BRR2 CBC2 CDC40 CWC15 CWC2 CWC22 CWC23 DHR2 LSM4 MAK5 MUD2 PRP16 PRP18 PRP2 PRP22 PRP24 PRP28 PRP31 PRP38 PRP39 PRP4 PRP40 PRP43 PRP5 PRP6 PRP8 SLU7 SMD1 SMD3 SME1 SNP1 SPP2 SPP381 STO1 YHC1
    View Details Krogan NJ, et al. (2006) AAR2 BRR1 BRR2 CSE1 LUC7 MUD1 NAM8 PRP2 PRP3 PRP31 PRP39 PRP40 PRP42 PRP6 PRP8 RRG7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNU114 SNU56 SNU66 SNU71 TAD2 YDL085C-A YHC1 YJU2 YNL092W
    View Details Gavin AC, et al. (2006) BRR2 CUS1 LEA1 PRP21 PRP3 PRP31 PRP4 PRP6 PRP8 PRP9 SMB1 SNU114 SNU23 SNU66 SPP381
    View Details Gavin AC, et al. (2002) AAR2 BRR1 BUD31 CDC33 CEF1 CLF1 CSN12 CUS1 CWC2 CWC22 CWC23 DHH1 DIB1 ECM2 GCN2 HSH155 HTA1 IMG1 LEA1 LIN1 LSM1 LSM2 LSM4 LSM5 LSM6 LSM7 LUC7 MRP7 MRPL28 MRPL3 MRPL35 MRPL38 MRPL4 MRPL8 MRPS5 MSL1 MUD1 PAT1 PRP11 PRP18 PRP19 PRP21 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP46 PRP6 PRP8 PRP9 RSE1 RVB2 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP381 SPP382 SRB2 STO1 SYF1 TOS4 UTP21 YML6
    View Details Ho Y, et al. (2002) COR1 DOG1 ENP1 FET4 FPR3 HAS1 MAM33 PIM1 PRP8 QCR2 SAP185 SAP190 SIT4 SNP1 SRP1 VAC14
    View Details Qiu et al. (2008) AAR2 BRR1 BRR2 BUD31 CBC2 CDC40 CEF1 CLF1 CUS1 CUS2 CWC15 CWC2 CWC21 CWC22 CWC23 CWC25 DHR2 DIB1 ECM2 HSH155 HSH49 IST3 ISY1 LEA1 LSM2 LSM3 LSM4 LSM5 LSM6 LSM7 LSM8 LUC7 MAK5 MSL1 MSL5 MUD1 MUD2 NAM8 NTC20 PRP11 PRP16 PRP18 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP46 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP2 SPP381 SPP382 STO1 SUB2 SYF1 SYF2 YHC1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CWC2 No Comments Hazbun TR, et al. (2003)
    View Run SPP382 No Comments Hazbun TR, et al. (2003)
    View Run SPP382 No Comments Hazbun TR, et al. (2003)
    View Run CWC25 No Comments Hazbun TR, et al. (2003)
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data PRP8 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..237] MSA 221.280999 View MSA. No confident structure predictions are available.
    2 View Details [238..803] deduced N/A No confident structure predictions are available.
    3 View Details [804..1022] MSA 1.014889 View MSA. No confident structure predictions are available.
    4 View Details [1023..1282] deduced N/A No confident structure predictions are available.
    5 View Details [1283..2068] MSA 1.022001 View MSA. No confident structure predictions are available.
    6 View Details [2069..2151] deduced N/A Confident ab initio structure predictions are available.
    7 View Details [2152..2322] MSA 15.053996 View MSA. No confident structure predictions are available.
    8 View Details [2323..2413] deduced N/A No confident structure predictions are available.
    9 View Details [1331..1527] deduced N/A No confident structure predictions are available.
    10 View Details [1528..1578] MSA 3.042987 View MSA. No confident structure predictions are available.
    11 View Details [1579..1650] MSA 1.039962 View MSA. No confident structure predictions are available.
    12 View Details [1651..1743] deduced N/A No confident structure predictions are available.
    13 View Details [1744..1998] MSA 1.037955 View MSA. No confident structure predictions are available.
    14 View Details [1999..2070] deduced N/A No confident structure predictions are available.
    15 View Details [2071..2413] PSI-BLAST 93.69897 No description for 2og4A was found.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle