Protein Overview: PRP2

General Information:
Name(s) found:	PRP2 / YNR011C [SGD]
Description(s) found: Found 30 descriptions. SHOW ALL	RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing [SGD]
Organism:	Saccharomyces cerevisiae
Length:	876 amino acids
Gene Ontology:
Cellular Component:	nucleus [TAS] spliceosomal complex [TAS]
Biological Process:	RNA splicing [IEA] generation of catalytic spliceosome for first transesterification step [IDA] mRNA processing [IEA]
Molecular Function:	ATP-dependent RNA helicase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSITSETGK RRVKRTYEVT RQNDNAVRIE PSSLGEEEDK EAKDKNSALQ LKRSRYDPNK  60
   61 VFSNTNQGPE KNNLKGEQLG SQKKSSKYDE KITSNNELTT KKGLLGDSEN ETKYASSNSK 120
  121 FNVEVTHKIK NAKEIDKINR QRMWEEQQLR NAMAGQSDHP DDITLEGSDK YDYVFDTDAM 180
  181 IDYTNEEDDL LPEEKLQYEA RLAQALETEE KRILTIQEAR KLLPVHQYKD ELLQEIKKNQ 240
  241 VLIIMGETGS GKTTQLPQYL VEDGFTDQGK LQIAITQPRR VAATSVAARV ADEMNVVLGK 300
  301 EVGYQIRFED KTTPNKTVLK YMTDGMLLRE FLTDSKLSKY SCIMIDEAHE RTLATDILIG 360
  361 LLKDILPQRP TLKLLISSAT MNAKKFSEFF DNCPIFNVPG RRYPVDIHYT LQPEANYIHA 420
  421 AITTIFQIHT TQSLPGDILV FLTGQEEIER TKTKLEEIMS KLGSRTKQMI ITPIYANLPQ 480
  481 EQQLKIFQPT PENCRKVVLA TNIAETSLTI DGIRYVIDPG FVKENSYVPS TGMTQLLTVP 540
  541 CSRASVDQRA GRAGRVGPGK CFRIFTKWSY LHELELMPKP EITRTNLSNT VLLLLSLGVT 600
  601 DLIKFPLMDK PSIPTLRKSL ENLYILGALN SKGTITRLGK MMCEFPCEPE FAKVLYTAAT 660
  661 HEQCQGVLEE CLTIVSMLHE TPSLFIGQKR DAAASVLSEV ESDHILYLEI FNQWRNSKFS 720
  721 RSWCQDHKIQ FKTMLRVRNI RNQLFRCSEK VGLVEKNDQA RMKIGNIAGY INARITRCFI 780
  781 SGFPMNIVQL GPTGYQTMGR SSGGLNVSVH PTSILFVNHK EKAQRPSKYV LYQQLMLTSK 840
  841 EFIRDCLVIP KEEWLIDMVP QIFKDLIDDK TNRGRR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		PRP2 PRP45
View Details	(MIPS) Mewes HW, et al. (2004)		AAR2 BRR1 BRR2 CBC2 CDC40 CWC15 CWC2 CWC22 CWC23 DHR2 LSM4 MAK5 MUD2 PRP16 PRP18 PRP2 PRP22 PRP24 PRP28 PRP31 PRP38 PRP39 PRP4 PRP40 PRP43 PRP5 PRP6 PRP8 SLU7 SMD1 SMD3 SME1 SNP1 SPP2 SPP381 STO1 YHC1
View Details	Krogan NJ, et al. (2006)		AAR2 BRR1 BRR2 CSE1 LUC7 MUD1 NAM8 PRP2 PRP3 PRP31 PRP39 PRP40 PRP42 PRP6 PRP8 RRG7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNU114 SNU56 SNU66 SNU71 TAD2 YDL085C-A YHC1 YJU2 YNL092W
View Details	Gavin AC, et al. (2002)		BUD31 CDC40 CEF1 CLF1 CWC2 CWC22 CWC23 ECM2 HSH155 ISY1 LEA1 PRP19 PRP2 PRP21 PRP22 PRP43 PRP45 PRP46 RSE1 SMD2 SNT309 SNU114 SPP382 STO1 SYF1
View Details	Qiu et al. (2008)		AAR2 BRR1 BRR2 BUD31 CBC2 CDC40 CEF1 CLF1 CUS1 CUS2 CWC15 CWC2 CWC21 CWC22 CWC23 CWC25 DHR2 DIB1 ECM2 GTS1 HSH155 IST3 ISY1 LEA1 LSM2 LSM3 LSM4 LSM5 LSM6 LSM7 LSM8 LUC7 MAK5 MSL1 MUD1 MUD2 NAM8 NTC20 PAB1 PRP11 PRP16 PRP18 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP46 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP2 SPP381 SPP382 STO1 SUB2 SYF1 SYF2 YHC1 YJU2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	PRP2	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
	4.704140727858	bayes_pls_golite062009
binding	2.44689197844883	bayes_pls_golite062009
RNA binding	2.29590582680214	bayes_pls_golite062009
structural constituent of ribosome	1.57653963868443	bayes_pls_golite062009
RNA helicase activity	1.42791449104435	bayes_pls_golite062009
nucleic acid binding	1.41477043612798	bayes_pls_golite062009
RNA-dependent ATPase activity	1.39335733374328	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.36724390116598	bayes_pls_golite062009
DNA binding	1.11699929651302	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
transcription regulator activity	0.989226939888218	bayes_pls_golite062009
mRNA binding	0.76699944561918	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.652333406433794	bayes_pls_golite062009
helicase activity	0.6191010101321	bayes_pls_golite062009
motor activity	0.61636596887462	bayes_pls_golite062009
structural molecule activity	0.596382945962261	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.461763181659193	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.458831409927227	bayes_pls_golite062009
pyrophosphatase activity	0.455976987338331	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.422944846459286	bayes_pls_golite062009
snRNA binding	0.418985924846052	bayes_pls_golite062009
purine NTP-dependent helicase activity	0.29664013896754	bayes_pls_golite062009
ATP-dependent helicase activity	0.29664013896754	bayes_pls_golite062009
translation release factor activity	0.178286731022211	bayes_pls_golite062009
translation termination factor activity	0.151456842619444	bayes_pls_golite062009
nucleotide binding	0.114848452385726	bayes_pls_golite062009
purine nucleotide binding	0.10713474095071	bayes_pls_golite062009
purine ribonucleotide binding	0.0916519414118148	bayes_pls_golite062009
ribonucleotide binding	0.0915913646217636	bayes_pls_golite062009
protein binding	0.071010918255742	bayes_pls_golite062009
transcription factor activity	0.0434163635422169	bayes_pls_golite062009
cytoskeletal protein binding	0.0272673919333979	bayes_pls_golite062009

No functions predicted.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..165]	MSA	2.520994	View MSA. No confident structure predictions are available.
2	View Details	[166..600]	PSI-BLAST	68.09691	Initiation factor 4a
3	View Details	[601..697]	MSA	1.52099	View MSA. Confident ab initio structure predictions are available.
4	View Details	[698..876]	MSA	3.169976	View MSA. No confident structure predictions are available.

Protein Overview: PRP2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: