Protein Overview: DBP8

General Information:
Name(s) found:	DBP8 / YHR169W [SGD]
Description(s) found: Found 30 descriptions. SHOW ALL	ATPase, putative RNA helicase of the DEAD-box family; component of 90S preribosome complex involved in production of 18S rRNA and assembly of 40S small ribosomal subunit; ATPase activity stimulated by association with Esp2p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	431 amino acids
Gene Ontology:
Cellular Component:	nuclear outer membrane [IMP][TAS] nucleolus [IDA][TAS]
Biological Process:	ribosome biogenesis [RCA]
Molecular Function:	ATP-dependent RNA helicase activity [IMP][TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MADFKSLGLS KWLTESLRAM KITQPTAIQK ACIPKILEGR DCIGGAKTGS GKTIAFAGPM  60
   61 LTKWSEDPSG MFGVVLTPTR ELAMQIAEQF TALGSSMNIR VSVIVGGESI VQQALDLQRK 120
  121 PHFIIATPGR LAHHIMSSGD DTVGGLMRAK YLVLDEADIL LTSTFADHLA TCISALPPKD 180
  181 KRQTLLFTAT ITDQVKSLQN APVQKGKPPL FAYQVESVDN VAIPSTLKIE YILVPEHVKE 240
  241 AYLYQLLTCE EYENKTAIIF VNRTMTAEIL RRTLKQLEVR VASLHSQMPQ QERTNSLHRF 300
  301 RANAARILIA TDVASRGLDI PTVELVVNYD IPSDPDVFIH RSGRTARAGR IGDAISFVTQ 360
  361 RDVSRIQAIE DRINKKMTET NKVHDTAVIR KALTKVTKAK RESLMAMQKE NFGERKRQQK 420
  421 KKQNDGKSLR S

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		DBP8 DOT1 IMH1 TRM1
View Details	Gavin AC, et al. (2006)		DBP8 ESF1 HCA4 MVD1 NOP56 RPL15A RPS16B, RPS16A
View Details	Gavin AC, et al. (2002)		ADE4 ASC1 BFR2 BMS1 BRX1 BUD21 CBF5 CFT2 CKA1 CKA2 CKB1 CKB2 CMS1 DBP8 DIA4 DIM1 DIP2 DIS3 ECM16 ECM29 EMG1 ENP1 ENP2 ERO1 ESF1 ESF2 FAP7 FIP1 GCN1 GLC7 HAS1 HCA4 HHF1, HHF2 HSL1 HTA2 IMP3 IMP4 KRE33 KRR1 LCP5 LTV1 MPP10 MRD1 MVD1 NAN1 NOB1 NOC2 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PFS2 PNO1 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 RCL1 REF2 RML2 RNA14 ROK1 RPP2B RRP12 RRP7 RRP9 RTG2 SAM1 SCL1 SEC21 SLX9 SOF1 SRO9 TIF1, TIF2 TSR1 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 YOR059C YSH1
View Details	Ho Y, et al. (2002)		CAR2 CDC15 CPA2 DBP8 HEF3 KGD1 OYE2 PFK1 PGM2 RNR1 RNR2 RPN1 SEC26 THI22 TIF1, TIF2 YDL086W
View Details	Qiu et al. (2008)		BRX1 DBP10 DBP3 DBP6 DBP7 DBP8 DHR2 DRS1 FAL1 GAR1 HCA4 KRR1 MAK5 MTR4 NAN1 NOP15 NSR1 NUG1 PRP43 PXR1 RNT1 SPB4 UTP13 UTP15 UTP4 UTP6 UTP8 UTP9

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ESF2	DBP8	2	Unpublished Fields Lab Data
View Screen	COF1	DBP8	1	Unpublished Fields Lab Data
View Screen	YOR203W	DBP8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	DBP8	Huh WK, et al. (2003)
View Data	DBP8 and NOP56	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..222]	PSI-BLAST	577.228787	Putative DEAD box RNA helicase
2	View Details	[223..431]	PSI-BLAST	577.228787	Putative DEAD box RNA helicase

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2