Protein Overview: ESF2

General Information:
Name(s) found:	ESF2 / YNR054C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324382\|ref\|NP_014452.1\| Essential nucleolar protein involved in pre-18S rRNA processing; component of the small subunit (SSU) processome; has sequence similarity to mABT1, a mouse transcription activator; Esf2p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S63385 hypothetical protein YNR054c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1730686\|sp\|P53743.1\|ESF2_YEAST RecName: Full=Pre-rRNA-processing protein ESF2; AltName: Full=18S rRNA factor 2 [NCBI NR] gi\|1302572\|emb\|CAA96335.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] Uncharacterized protein YNR054C - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] Pre-rRNA-processing protein ESF2 OS=Saccharomyces cerevisiae GN=ESF2 PE=1 SV=1 [Swiss-Prot] ESF2 Eighteen S rRNA processing Factor [mips] Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome [SGD] (P53743) Hypothetical 36.4 kDa protein in POP2-HOL1 intergenic region [4932.FASTACCERV.txt] Chr XIV from 724302-723352, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XIV from 723352-724302, reverse complement [yeastorf3.fasta] YNR054C, Chr XIV from 723352-724302, reverse complement [yeast_orfs2] YNR054C SGDID:S0005337, Chr XIV from 724304-723354, reverse complement, Uncharacterized ORF [yeastflysequest.fasta] ESF2 SGDID:S0005337, Chr XIV from 724305-723355, reverse complement, Uncharacterized ORF [10112005cervSGD.fasta] Esf2p [Saccharomyces cerevisiae]gi\|1302572\|emb\|CAA96335.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|2131979\|pir\|\|S63385 hypothetical protein YNR054c - yeast (Saccharomyces cerevisiae)gi\|1730686\|sp\|P53743\|YN8V_YEAST Hypothetical 36.4 kDa pro [nrdb_11152004_Chlamydomonas] ESF2 SGDID:S000005337, Chr XIV from 724307-723357, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; component of the small subunit (SSU) processome; has sequence similarity to mABT1, a mouse transcription [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ESF2 SGDID:S000005337, Chr XIV from 724307-723357, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) pro [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Essential nucleolar protein involved in pre-18S rRNA processing; component of the small subunit (SSU) processome; has sequence similarity to mABT1, a mouse transcription activator; Esf2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Uncharacterized protein YNR054C [nr-271106-contam.fasta] ESF2 SGDID:S000005337, Chr XIV from 724308-723358, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) pro [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] ESF2 SGDID:S000005337, Chr XIV from 724307-723357, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome" [yeast-bovrabprots.fasta] ESF2 SGDID:S000005337, Chr XIV from 724307-723357, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; component of the small subunit (SSU) processome; has sequence similarity to mABT1, a mouse transcription activator" [yeast-contam_custom.fasta] [chemostatdb.fasta] ESF2 SGDID:S000005337, Chr XIV from 724308-723358, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome" [SGDyeast.20101221.fasta] Pre-rRNA-processing protein ESF2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ESF2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ESF2 SGDID:S000005337, Chr XIV from 724306-723356, Genome Release 64-1-1, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome" [yeast-030211-contam.fasta] ESF2 SGDID:S000005337, Chr XIV from 724306-723356, Genome Release 64-2-1, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome" [20141208_orf_trans.fasta] YNR054C SGDID:S000005337, Chr XIV from 724306-723356, Genome Release 64-1-1, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	316 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA ] nuclear outer membrane [IMP] nucleolus [IDA ] small nucleolar ribonucleoprotein complex [IDA][IPI]
Biological Process:	ribosome biogenesis [RCA]
Molecular Function:	transcription regulator activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSEKVNSDFE DFSSDEETDQ HNVLIQTKKK ISSKDDIFSK KVEDIESENE SDIEEEQKQE  60
   61 EKEDVEQPDK ENGEKLDREV EEQASSTTSL DLKTEKLRQL VKSKAAKKSK HKTGVVYFSS 120
  121 IPPYMKPAKM RQILTRFGEV DRLFLKKEDD QKYKQRVKGG GNKKNKYEEG WAEFIRKRDA 180
  181 KLCAETLNGN IIGGKKGTFY HDDILNVKYL PGFKWADLTE QIARENDIRQ AKLEMEISQA 240
  241 NKLNAEFIRN VEQSKMIQNI KNSRKRAGKE GESADSHPHR EFKQRRVETS RANAPSDIKQ 300
  301 QSSGSKDLGN VLTNLL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BFR2 ENP2 ESF1 ESF2 HCA4 RPS11A, RPS11B RRP3
View Details	Gavin AC, et al. (2002)		ADE4 ASC1 BFR2 BMS1 BRX1 BUD21 CBF5 CFT2 CKA1 CKA2 CKB1 CKB2 CMS1 DBP8 DIA4 DIM1 DIP2 DIS3 ECM16 ECM29 EMG1 ENP1 ENP2 ERO1 ESF1 ESF2 FAP7 FIP1 GCN1 GLC7 HAS1 HCA4 HHF1, HHF2 HSL1 HTA2 IMP3 IMP4 KRE33 KRR1 LCP5 LTV1 MPP10 MRD1 MVD1 NAN1 NOB1 NOC2 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PFS2 PNO1 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 RCL1 REF2 RML2 RNA14 ROK1 RPP2B RRP12 RRP7 RRP9 RTG2 SAM1 SCL1 SEC21 SLX9 SOF1 SRO9 TIF1, TIF2 TSR1 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 YOR059C YSH1
View Details	Ho Y, et al. (2002)		CDC33 CKA1 CKA2 CKB1 ESF1 ESF2 HTB1 IMD3 LAA1 LHP1 MSS116 NOP12 PMA1 RPF2 YCR087W YDR102C YRA1
View Details	Qiu et al. (2008)		ESF2 GAR1 NUG1 PXR1 UTP9

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ESF1	ESF2	2	Hazbun TR, et al. (2003)
View Screen	ESF2	YDR026C	2	Unpublished Fields Lab Data
View Screen	ESF2	ESF1	2	Unpublished Fields Lab Data
View Screen	ESF2	YDR532C	2	Unpublished Fields Lab Data
View Screen	ESF2	DBP8	2	Unpublished Fields Lab Data
View Screen	ESF2	SMX3	1	Unpublished Fields Lab Data
View Screen	RPB2	ESF2	1	Unpublished Fields Lab Data
View Screen	ESF1	ESF2	1	Hazbun TR, et al. (2003)
View Screen	ESF2	AHC2	1	Unpublished Fields Lab Data
View Screen	ESF2	SLD5	1	Unpublished Fields Lab Data
View Screen	ESF2	YER079W	1	Unpublished Fields Lab Data
View Screen	ESF2	TIF1, TIF2	1	Unpublished Fields Lab Data
View Screen	ESF2	AHC1	1	Unpublished Fields Lab Data
View Screen	ESF2	MAK3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	ESF2	Huh WK, et al. (2003)
View Data	ESF2 and NOP56	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..98]	PSI-BLAST	23.0	Nuclear ribonucleoprotein A1 (RNP A1, UP1)
2	View Details	[99..197]	PSI-BLAST	23.0	Nuclear ribonucleoprotein A1 (RNP A1, UP1)
3	View Details	[198..265]	PSI-BLAST	7.69897	Polypyrimidine tract-binding protein
4	View Details	[266..316]	PSI-BLAST	3.69897	poly(A) binding protein

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2