Protein Overview: MCM5

General Information:
Name(s) found:	MCM5 / YLR274W [SGD]
Description(s) found: Found 31 descriptions. SHOW ALL	Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase [SGD]
Organism:	Saccharomyces cerevisiae
Length:	775 amino acids
Gene Ontology:
Cellular Component:	replication fork [IDA] pre-replicative complex [IDA] cytoplasm [TAS] MCM complex [IDA]
Biological Process:	DNA replication initiation [TAS] establishment of chromatin silencing [IMP] pre-replicative complex assembly [IPI] DNA unwinding involved in replication [TAS]
Molecular Function:	chromatin binding [TAS] ATP-dependent DNA helicase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFDRPEIYS APVLQGESPN DDDNTEIIKS FKNFILEFRL DSQFIYRDQL RNNILVKNYS  60
   61 LTVNMEHLIG YNEDIYKKLS DEPSDIIPLF ETAITQVAKR ISILSRAQSA NNNDKDPENT 120
  121 SMDTDSLLLN SLPTFQLILN SNANQIPLRD LDSEHVSKIV RLSGIIISTS VLSSRATYLS 180
  181 IMCRNCRHTT SITINNFNSI TGNTVSLPRS CLSTIESESS MANESNIGDE STKKNCGPDP 240
  241 YIIIHESSKF IDQQFLKLQE IPELVPVGEM PRNLTMTCDR YLTNKVIPGT RVTIVGIYSI 300
  301 YNSKNGAGSG RSGGGNGGSG VAIRTPYIKI LGIQSDVETS SIWNSVTMFT EEEEEEFLQL 360
  361 SRNPKLYEIL TNSIAPSIFG NEDIKKAIVC LLMGGSKKIL PDGMRLRGDI NVLLLGDPGT 420
  421 AKSQLLKFVE KVSPIAVYTS GKGSSAAGLT ASVQRDPMTR EFYLEGGAMV LADGGVVCID 480
  481 EFDKMRDEDR VAIHEAMEQQ TISIAKAGIT TVLNSRTSVL AAANPIYGRY DDLKSPGDNI 540
  541 DFQTTILSRF DMIFIVKDDH NEERDISIAN HVINIHTGNA NAMQNQQEEN GSEISIEKMK 600
  601 RYITYCRLKC APRLSPQAAE KLSSNFVTIR KQLLINELES TERSSIPITI RQLEAIIRIT 660
  661 ESLAKLELSP IAQERHVDEA IRLFQASTMD AASQDPIGGL NQASGTSLSE IRRFEQELKR 720
  721 RLPIGWSTSY QTLRREFVDT HRFSQLALDK ALYALEKHET IQLRHQGQNI YRSGV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		CDC45 CDC6 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32 SLD3
View Details	Gavin AC, et al. (2002)		CDC45 ERG26 GLT1 HAT1 HSP42 LYS12 MCM2 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 RPD3 RPN9 SIN3 TFP1
View Details	Ho Y, et al. (2002)		BMS1 CDC53 CIC1 COF1 CTF4 DBP10 DED81 DIA2 ENP1 FPR3 ILS1 KRE33 KRI1 LST4 MCM2 MCM3 MCM5 NIP7 NMD3 NOP12 RRP12 SEH1 SKP1 SLT2 SPB1 SSF1 SSF2 SSZ1 TIF6 URB1 UTP10 YAK1 YBL104C
View Details	Qiu et al. (2008)		CDC45 CDC6 DPB11 DPB2 DPB3 DPB4 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 PCC1 POB3 POL2 POL3 POL31 POL32 PRI2 SLD3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BRP1	MCM5	1	Unpublished Fields Lab Data
View Screen	RAD6	MCM5	1	Unpublished Fields Lab Data
View Screen	VAM7	MCM5	1	Unpublished Fields Lab Data
View Screen	IML1	MCM5	1	Unpublished Fields Lab Data
View Screen	CPR6	MCM5	1	Unpublished Fields Lab Data
View Screen	CTF13	MCM5	1	Unpublished Fields Lab Data
View Screen	PFS2	MCM5	1	Unpublished Fields Lab Data
View Screen	PHO85	MCM5	1	Unpublished Fields Lab Data
View Screen	VIK1	MCM5	1	Unpublished Fields Lab Data
View Screen	CLB2	MCM5	1	Unpublished Fields Lab Data
View Screen	RVS167	MCM5	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..122]	MSA	2.095995	View MSA. No confident structure predictions are available.
2	View Details	[123..373]	Pfam	8.823909	MCM2/3/5 family No confident structure predictions are available.
3	View Details	[374..606]	PSI-BLAST	44.522879	ATPase subunit of magnesium chelatase, BchI
4	View Details	[607..775]	PSI-BLAST	44.522879	ATPase subunit of magnesium chelatase, BchI

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
telomeric DNA binding	4.81350293592083	bayes_pls_golite062009
telomerase inhibitor activity	4.48082903165152	bayes_pls_golite062009
single-stranded telomeric DNA binding	4.34033743483519	bayes_pls_golite062009
nucleic acid binding	4.29626483284963	bayes_pls_golite062009
DNA binding	3.71771169141041	bayes_pls_golite062009
structural constituent of ribosome	3.51998515668903	bayes_pls_golite062009
structure-specific DNA binding	3.48139490984491	bayes_pls_golite062009
single-stranded DNA binding	3.43027351695641	bayes_pls_golite062009
structural molecule activity	3.29857127235306	bayes_pls_golite062009
binding	2.83465364476231	bayes_pls_golite062009
transcription regulator activity	2.10110937001387	bayes_pls_golite062009
sequence-specific DNA binding	1.9581702424972	bayes_pls_golite062009
DNA replication origin binding	1.84819558112187	bayes_pls_golite062009
translation regulator activity	1.46320772716954	bayes_pls_golite062009
translation factor activity, nucleic acid binding	1.40718318783582	bayes_pls_golite062009
transcription repressor activity	1.30343463892105	bayes_pls_golite062009
translation initiation factor activity	1.27539679024898	bayes_pls_golite062009
transcription activator activity	1.24141936405635	bayes_pls_golite062009
protein binding	1.20309946451799	bayes_pls_golite062009
RNA polymerase activity	1.19766815287926	bayes_pls_golite062009
DNA-directed RNA polymerase activity	1.19766815287926	bayes_pls_golite062009
ribonuclease activity	1.19236946227657	bayes_pls_golite062009
transcription factor activity	1.14242112812892	bayes_pls_golite062009
DNA helicase activity	1.12203697857261	bayes_pls_golite062009
DNA-dependent ATPase activity	0.638831894247026	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.635129001787169	bayes_pls_golite062009
nuclease activity	0.57381531077345	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	0.474251377131502	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	0.47336621289748	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	0.47336621289748	bayes_pls_golite062009
nucleotidyltransferase activity	0.325774164899685	bayes_pls_golite062009
four-way junction helicase activity	0.31769813397725	bayes_pls_golite062009
double-stranded DNA binding	0.314881439089948	bayes_pls_golite062009
exonuclease activity	0.28313884527829	bayes_pls_golite062009
transferase activity	0.249054945579328	bayes_pls_golite062009
RNA binding	0.18502033889261	bayes_pls_golite062009
exoribonuclease activity, producing 5'-phosphomonoesters	0.125256208482441	bayes_pls_golite062009
exoribonuclease activity	0.109774918833391	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.0954216620147701	bayes_pls_golite062009
DEAD/H-box RNA helicase binding	0.0627405398613412	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.0179535526984695	bayes_pls_golite062009
helicase activity	0.0121541775159524	bayes_pls_golite062009

Term	Confidence	Notes
DNA clamp loader activity	4.91429252297969	bayes_pls_golite062009
protein-DNA loading ATPase activity	4.80695929076807	bayes_pls_golite062009
DNA-dependent ATPase activity	2.72462789867829	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	2.66107861968804	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
nucleic acid binding	2.51683451033161	bayes_pls_golite062009
DNA helicase activity	2.2817164193758	bayes_pls_golite062009
pyrophosphatase activity	2.13284212711072	bayes_pls_golite062009
DNA binding	2.12258000781149	bayes_pls_golite062009
transcription regulator activity	2.09345198747025	bayes_pls_golite062009
telomeric DNA binding	2.0142766759353	bayes_pls_golite062009
ATP-dependent DNA helicase activity	1.88455339138556	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
microtubule motor activity	1.6537049553461	bayes_pls_golite062009
DNA replication origin binding	1.62491387774159	bayes_pls_golite062009
ribonucleotide binding	1.53142082854758	bayes_pls_golite062009
purine nucleotide binding	1.52938239070386	bayes_pls_golite062009
purine ribonucleotide binding	1.52887308780164	bayes_pls_golite062009
nucleotide binding	1.52746968836881	bayes_pls_golite062009
structural constituent of ribosome	1.47858140861683	bayes_pls_golite062009
ATP binding	1.23942044024113	bayes_pls_golite062009
protein binding	1.22129154759482	bayes_pls_golite062009
adenyl ribonucleotide binding	1.21044170258803	bayes_pls_golite062009
structure-specific DNA binding	1.20315703345493	bayes_pls_golite062009
adenyl nucleotide binding	1.19481945569892	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
motor activity	1.14667913749579	bayes_pls_golite062009
kinase activity	1.11120928120082	bayes_pls_golite062009
lysine N-acetyltransferase activity	1.05990253628867	bayes_pls_golite062009
histone acetyltransferase activity	1.05990253628867	bayes_pls_golite062009
double-stranded DNA binding	1.05408274676326	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.971476020912368	bayes_pls_golite062009
DNA polymerase activity	0.928091843881235	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.908046791062776	bayes_pls_golite062009
transcription activator activity	0.889929694023098	bayes_pls_golite062009
transcription factor activity	0.887108427603557	bayes_pls_golite062009
structural molecule activity	0.86514677273731	bayes_pls_golite062009
transcription repressor activity	0.862685397469217	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.8469248456592	bayes_pls_golite062009
single-stranded telomeric DNA binding	0.700780808446722	bayes_pls_golite062009
sequence-specific DNA binding	0.543891899378373	bayes_pls_golite062009
cytoskeletal protein binding	0.500462538667228	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.421921195209539	bayes_pls_golite062009
single-stranded DNA binding	0.406600732518248	bayes_pls_golite062009
protein kinase activity	0.351308564667977	bayes_pls_golite062009
translation regulator activity	0.29096092258568	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.274994961137851	bayes_pls_golite062009
four-way junction helicase activity	0.26252931460003	bayes_pls_golite062009
exodeoxyribonuclease V activity	0.185603938981231	bayes_pls_golite062009
RNA helicase activity	0.18426050934025	bayes_pls_golite062009
transferase activity	0.175269600441384	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	0.156278588350022	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.147579541298736	bayes_pls_golite062009
RNA-dependent ATPase activity	0.14606996093053	bayes_pls_golite062009
mismatched DNA binding	0.13366911641814	bayes_pls_golite062009
ATP-dependent RNA helicase activity	0.13050483816765	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.113504049290503	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.111293884322157	bayes_pls_golite062009
3'-5' DNA helicase activity	0.0769112340058804	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	0.0223627159838333	bayes_pls_golite062009
actin binding	0.00482938540552702	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2