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Protein Overview: YHR020W

General Information:
Name(s) found: YHR020W [SGD]
Description(s) found:
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  • gi|731640|sp|P38708.1|YHI0_YEAST RecName: Full=Putative prolyl-tRNA synthetase YHR020W; AltName: Full=Proline--tRNA ligase; Short=ProRS [NCBI NR]
  • gi|6321808|ref|NP_011884.1| Protein of unknown function that may interact with ribosomes, based on co-purification experiments; has similarity to proline-tRNA ligase; YHR020W is an essential gene; Yhr020wp [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S46774 multifunctional amino acid-tRNA ligase homolog - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|500692|gb|AAB68873.1| Yhr020wp [Saccharomyces cerevisiae] [NCBI NR]
  • Putative prolyl-tRNA synthetase YHR020W OS=Saccharomyces cerevisiae GN=YHR020W PE=1 SV=1 [Swiss-Prot]
  • Protein required for cell viability, putative glutamyl-prolyl-tRNA synthetase [mips]
  • Protein of unknown function that may interact with ribosomes, based on co-purification experiments; has similarity to proline-tRNA ligase; YHR020W is an essential gene [SGD]
  • YHR020W, Chr VIII from 143987-146053 [yeast_orfs2]
  • Chr VIII from 143987-146053 [yeastorf3.fasta]
  • (P38708) Putative prolyl-tRNA synthetase YHR020W (EC 6.1.1.15) (Proline--tRNA ligase) (ProRS) [4932.FASTACCERV.txt]
  • YHR020W SGDID:S0001062, Chr VIII from 143988-146054, Uncharacterized ORF [yeastflysequest.fasta]
  • Protein required for cell viability [Saccharomyces cerevisiae]gi|500692|gb|AAB68873.1| Yhr020wp [Saccharomyces cerevisiae]gi|626755|pir||S46774 multifunctional amino acid-tRNA ligase homolog - yeast (Saccharomyces cerevisiae)gi|731640|sp|P38708|YHI0_Y [nrdb_11152004_Chlamydomonas]
  • YHR020W SGDID:S000001062, Chr VIII from 143989-146055, Uncharacterized ORF, "Protein required for cell viability" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • YHR020W SGDID:S000001062, Chr VIII from 143989-146055, Uncharacterized ORF, "Putative protein of unknown function, has similarity to proline-tRNA ligase; YHR020W is an essential gene" [yeast-contam.fasta]
  • YHR020W SGDID:S000001062, Chr VIII from 143989-146055, Verified ORF, "Protein of unknown function that may interact with ribosomes, based on co-purification experiments; has similarity to proline-tRNA ligase; YHR020W is an essential gene" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta]
  • Putative prolyl-tRNA synthetase YHR020W (Proline--tRNA ligase) (ProRS) [nr-271106-contam.fasta]
  • Yhr020wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Putative protein of unknown function, has similarity to proline-tRNA ligase; YHR020W is an essential gene; Yhr020wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • [chemostatdb.fasta]
  • Putative proline--tRNA ligase YHR020W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YHR020W PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • YHR020W SGDID:S000001062, Chr VIII from 143996-146062, Genome Release 64-1-1, Verified ORF, "Protein of unknown function that may interact with ribosomes, based on co-purification experiments; has similarity to proline-tRNA ligase; YHR020W is an essential gene" [yeast-030211-contam.fasta]
  • YHR020W SGDID:S000001062, Chr VIII from 143996-146062, Genome Release 64-2-1, Verified ORF, "Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments" [20141208_orf_trans.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 688 amino acids

    Gene Ontology:
    Cellular Component: ribosome [IDALink to PubMed article]
    Biological Process: tRNA aminoacylation for protein translation [ISSLink to PubMed article]
    Molecular Function: proline-tRNA ligase activity [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPVSEAFAKL CVNEKPPAES AVAVKSLVFK PKTPKSATPV PIVVVALQST TTPSALIANA  60
   61 TSSKDPRLAR DDLVKQAFQS ESARAFILGD LANATSNFHL LIDHELGTVD GDTILQLNDS 120
  121 TYMKKSDMMK FLNNFEDSQK VVDFSQEVSK ETATEGKKQQ KKQQPSKAGT AAAAAAAALE 180
  181 DAKLIGITVD KALDFPGWYQ QILTKGEMLD YYDVSGCYIL RPPSYAIWEN IQKWFDDKIK 240
  241 AIGVQNAYFP MFVSSRVLEK EKDHVEGFAP EVAWVTRAGS SELEEPIAIR PTSETVMYPY 300
  301 YAKWVQSYRD LPLKLNQWNS VVRWEFKHPQ PFLRTREFLW QEGHTAHLTA KDAEEEVLQI 360
  361 LDFYAGVYEE LLAVPVVKGR KTEKEKFAGG DFTTTCEGYI PQTGRGIQGA TSHHLGQNFS 420
  421 KMFNLSVENP LGSDHPKIFA YQNSWGLSTR VIGVMVMIHS DNKGLVIPPR VSQFQSVVIP 480
  481 VGITKKTSEE QRKHIHETAR SVESRLKKVG IRAFGDYNDN YTPGWKFSQY ELKGIPIRIE 540
  541 LGPKDIEKNQ VVVVRRNDSK KYVVSFDELE ARIPEILEEM QGDLFKKAKE LFDTHRVIVN 600
  601 EWSGFVPALN KKNVILAPWC GVMECEEDIK ESSAKKDDGE EFEEDDKAPS MGAKSLCIPF 660
  661 DQPVLNEGQK CIKCERIAVN YCMFGRSY

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ATP2 CLU1 CRM1 EFT2, EFT1 FKS1 GCN20 GEA2 HSC82 ILV1 KAP104 MIR1 SAM1 YHR020W
    View Details Gavin AC, et al. (2002) ADH1 BMH1 BMH2 CCT4 CLU1 CRM1 CSE1 DHH1 DUG1 ECM29 FKS1 GCN1 GCN20 GEA2 GFA1 HRP1 HSP42 IDH2 KAP104 KAP123 KTR3 LCB1 LCB2 LYS12 MIR1 NAB2 NSL1 PDC1 PRP11 RGR1 SAM1 SAM2 SEC7 SLC1 SRV2 TAT1 YHR020W
    View Details Ho Y, et al. (2002) AAC1 AAC3 ADE5,7 ADH2 AHA1 ARC1 ARO1 ARP10 ARP2 ATG11 ATG12 ATG17 CAR2 CPA2 CPR6 DIP2 DIP5 FET4 GCD11 GFA1 GUS1 HSP104 IPP1 MUM2 PGM2 POR1 PPX1 PRB1 PTC7 REP1 REX2 RFC1 RFC2 RFC4 RPN10 RPN11 RPN3 RPN5 RPN6 RPN7 RPT1 RPT3 SEC18 TCB1 TFP1 TIF1, TIF2 TYR1 UTP13 UTP18 YHR020W YHR033W YNL208W

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run TIM50 No Comments Hazbun TR, et al. (2003)
    View Run YNL313C No Comments Hazbun TR, et al. (2003)
    View Run PGA1 No Comments Hazbun TR, et al. (2003)
    View Run KAE1 No Comments Hazbun TR, et al. (2003)
    View Run MIA40 No Comments Hazbun TR, et al. (2003)
    View Run HIR2 No Comments Green EM, et al (2005)
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run ASF1 sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant) Green EM, et al (2005)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run MLP2 #25 Asynchronous Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run None Entered #17 Mitotic Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..586] PSI-BLAST 143.9897 Threonyl-tRNA synthetase (ThrRS), C-terminal domain; Threonyl-tRNA synthetase (ThrRS), N-terminal 'additional' domain; Threonyl-tRNA synthetase (ThrRS), second 'additional' domain; Threonyl-tRNA synthetase (ThrRS)
    2 View Details [587..688] PSI-BLAST 1170.0 Prolyl-tRNA synthetase (ProRS) domain; C-terminal domain of ProRS; Prolyl-tRNA synthetase (ProRS)
    3 View Details [605..688] PSI-BLAST 116.0 Prolyl-tRNA synthetase (ProRS) domain; C-terminal domain of ProRS; Prolyl-tRNA synthetase (ProRS)

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • proline-tRNA ligase activity
    		•  7.67335999089351 bayes_pls_golite062009
  • ligase activity, forming aminoacyl-tRNA and related compounds
    		•  6.0520403280225 bayes_pls_golite062009
  • ligase activity, forming carbon-oxygen bonds
    		•  6.0520403280225 bayes_pls_golite062009
  • aminoacyl-tRNA ligase activity
    		•  6.04138870254626 bayes_pls_golite062009
  • ligase activity
    		•  4.85905957427768 bayes_pls_golite062009
  • phenylalanine-tRNA ligase activity
    		•  3.22631630581238 bayes_pls_golite062009
  • glycine-tRNA ligase activity
    		•  2.29229136935761 bayes_pls_golite062009
  • threonine-tRNA ligase activity
    		•  2.18642354927 bayes_pls_golite062009
  • serine-tRNA ligase activity
    		•  2.07594942154162 bayes_pls_golite062009
  • alanine-tRNA ligase activity
    		•  1.82594054955281 bayes_pls_golite062009
  • lysine-tRNA ligase activity
    		•  1.72324031407565 bayes_pls_golite062009
  • histidine-tRNA ligase activity
    		•  1.72215103383322 bayes_pls_golite062009
  • binding
    		•  1.30923742450698 bayes_pls_golite062009
  • asparagine-tRNA ligase activity
    		•  0.921492123574877 bayes_pls_golite062009
  • catalytic activity
    		•  0.902495165806215 bayes_pls_golite062009
  • RNA binding
    		•  0.576453255038334 bayes_pls_golite062009
  • protein binding
    		•  0.358563180490135 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.236290074198593 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.202044919116061 bayes_pls_golite062009
  • aspartate-tRNA ligase activity
    		•  0.00572514744734753 bayes_pls_golite062009
    3 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.96

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle