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Protein Overview: LSG1

General Information:
Name(s) found: LSG1 / YGL099W [SGD]
Description(s) found:
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  • gi|6321339|ref|NP_011416.1| Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm; Lsg1p [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S64106 hypothetical protein YGL099w - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|1723894|sp|P53145.1|LSG1_YEAST RecName: Full=Large subunit GTPase 1 [NCBI NR]
  • gi|1322637|emb|CAA96805.1| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR]
  • Uncharacterized GTP-binding protein YGL099W - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot]
  • Large subunit GTPase 1 OS=Saccharomyces cerevisiae GN=LSG1 PE=1 SV=1 [Swiss-Prot]
  • LSG1 Killer toxin REsistant [mips]
  • Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm [SGD]
  • KRE35, Chr VII from 314629-316551 [yeast_orfs2]
  • Chr VII from 314629-316551 [yeastorf3.fasta]
  • Chr VII from 314632-316554 [yeastorf4.fasta]
  • (P53145) Hypothetical GTP-binding protein in SEH1-PRP20 intergenic region [4932.FASTACCERV.txt]
  • LSG1 SGDID:S0003067, Chr VII from 314632-316554, Verified ORF [yeastflysequest.fasta]
  • LSG1 SGDID:S0003067, Chr VII from 314634-316556, Verified ORF [10112005cervSGD.fasta]
  • Lsg1p [Saccharomyces cerevisiae]gi|1322637|emb|CAA96805.1| unnamed protein product [Saccharomyces cerevisiae]gi|2131584|pir||S64106 hypothetical protein YGL099w - yeast (Saccharomyces cerevisiae)gi|1723894|sp|P53145|YGJ9_YEAST Hypothetical GTP-binding [nrdb_11152004_Chlamydomonas]
  • LSG1 SGDID:S000003067, Chr VII from 314634-316556, Verified ORF, "Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm; Lsg1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Uncharacterized GTP-binding protein YGL099W [nr-271106-contam.fasta]
  • [chemostatdb.fasta]
  • Large subunit GTPase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LSG1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • LSG1 SGDID:S000003067, Chr VII from 314631-316553, Genome Release 64-1-1, Verified ORF, "Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm" [yeast-030211-contam.fasta]
  • LSG1 SGDID:S000003067, Chr VII from 314631-316553, Genome Release 64-2-1, Verified ORF, "Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm" [20141208_orf_trans.fasta]
  • YGL099W SGDID:S000003067, Chr VII from 314631-316553, Genome Release 64-1-1, Verified ORF, "Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 640 amino acids

    Gene Ontology:
    Cellular Component: cytoplasm [IDALink to PubMed article]
    Biological Process: ascospore formation [IMPLink to PubMed article]
    ribosomal subunit export from nucleus [IMPLink to PubMed article][IPILink to PubMed article]
    conjugation with cellular fusion [IMPLink to PubMed article]
    ribosome biogenesis [RCALink to PubMed article]
    Molecular Function: GTPase activity [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPPKEAPKKW KAPKGPKPTH RKNKNKLELG RAIKYARQKE NAIEYLPDGE MRFTTDKHEA  60
   61 NWVKLRSVTQ ESALDEFLST AALADKDFTA DRHSNVKIIR MDSGNDSATS QGFSMTNEQR 120
  121 GNLNAKQRAL AKDLIVPRRP EWNEGMSKFQ LDRQEKEAFL EWRRKLAHLQ ESNEDLLLTP 180
  181 FERNIEVWKQ LWRVVERSDL VVQIVDARNP LLFRSVDLER YVKESDDRKA NLLLVNKADL 240
  241 LTKKQRIAWA KYFISKNISF TFYSALRANQ LLEKQKEMGE DYREQDFEEA DKEGFDADEK 300
  301 VMEKVKILSI DQLEELFLSK APNEPLLPPL PGQPPLINIG LVGYPNVGKS STINSLVGAK 360
  361 KVSVSSTPGK TKHFQTIKLS DSVMLCDCPG LVFPNFAYNK GELVCNGVLP IDQLRDYIGP 420
  421 AGLVAERIPK YYIEAIYGIH IQTKSRDEGG NGDIPTAQEL LVAYARARGY MTQGYGSADE 480
  481 PRASRYILKD YVNGKLLYVN PPPHLEDDTP YTREECEEFN KDLYVFDRLP DTRKEQVQNA 540
  541 AKAKGIDIVD LARDLNQLTF SAHTGGDTQK EAKSVTHGGK QAALYNAAED LDRDFFKMNN 600
  601 VEGRLSTPFH KVQNSSAGKR HNKKNKSKNA KSKVFSIENN

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ARX1 LSG1 NOG1 NOG2 NOP7 REI1
    View Details Krogan NJ, et al. (2006) FES1 LSG1 LYS2
    View Details Gavin AC, et al. (2006) LSG1 NMD3 REI1
    View Details Gavin AC, et al. (2002) ADE3 ARX1 ASC1 ATG9 BEM2 BMS1 BRX1 BUD20 CIC1 CLU1 CYR1 CYS4 DBP10 DBP2 DBP9 DCP2 DRS1 EBP2 ERB1 FPR4 FUN12 GCN1 GLT1 HAS1 HCR1 HDA1 HER1 HIS3 INP2 IPI1 IPI3 IST2 KRE33 KTR5 LHP1 LOC1 LSG1 MAK21 MAK5 MDN1 MIS1 MRT4 NAP1 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP7 NSA1 NSA2 NUG1 PRT1 PUF6 RAT1 REI1 RIC1 RIX1 RIX7 RLI1 RLP24 RLP7 RPA135 RPA190 RPF1 RPF2 RPG1 RPP2B RPT6 RRP1 RRP12 RSA3 RSA4 RTR1 SDA1 SEC7 SHM1 SKI2 SNU13 SPB1 SPB4 SPT5 SSF1 SUA7 SUI1 SUI2 SUI3 SUP45 TIF34 TIF35 TIF5 TIF6 TUB3 URB1 UTP10 UTP20 UTP22 UTP6 YGL036W YPL009C YTM1
    View Details Qiu et al. (2008) LSG1 RKI1 RPL24A RPL6B

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run BOI1 Sample boi1 with gst from october 2005 McCusker D, et al (2007)
    View Run BOI1 Sample bob1 (2nd set) from october 2005 McCusker D, et al (2007)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data LSG1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..102] MSA 1.102986 View MSA. No confident structure predictions are available.
    2 View Details [103..321] PSI-BLAST 2.522879 Initiation factor IF2/eIF5b, domains 2 and 4; Initiation factor IF2/eIF5b, domain 3; Initiation factor IF2/eIF5b, N-terminal (G) domain
    3 View Details [322..512] PSI-BLAST 41.0 GTPase Era, N-terminal domain; GTPase Era C-terminal domain
    4 View Details [513..640] PSI-BLAST 41.0 GTPase Era, N-terminal domain; GTPase Era C-terminal domain


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle