Protein Overview: CDC28

General Information:
Name(s) found:	CDC28 / YBR160W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|TVBY8 protein kinase (EC 2.7.1.37) cdc28 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6319636\|ref\|NP_009718.1\| Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; Cdc28p [Saccharomyces cerevisiae] [NCBI NR] gi\|536494\|emb\|CAA85119.1\| CDC28 [Saccharomyces cerevisiae] [NCBI NR] gi\|535469\|emb\|CAA56509.1\| protein kinase [Saccharomyces cerevisiae] [NCBI NR] gi\|3486\|emb\|CAA25065.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|223857\|prf\|\|1002252A protein CDC28 [NCBI NR] gi\|115915\|sp\|P00546.1\|CDC28_YEAST RecName: Full=Cell division control protein 28 [NCBI NR] Cell division control protein 28 OS=Saccharomyces cerevisiae GN=CDC28 PE=1 SV=1 [Swiss-Prot] (P00546) Cell division control protein 28 (EC 2.7.1.37) [Swiss-Prot] CDC28 Cyclin-dependent protein kinase [mips] Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates [SGD] CDC28, Chr II from 560035-560931 [yeast_orfs2] Chr II from 560035-560931 [yeastorf3.fasta] Chr II from 560034-560930 [yeastorf4.fasta] Chr II from 560032-560928 [yeastorf5.fasta] (P00546) Cell division control protein 28 (EC 2.7.1.-) [4932.FASTACCERV.txt] CDC28 SGDID:S0000364, Chr II from 560034-560930, Verified ORF [yeastflysequest.fasta] CDC28 SGDID:S0000364, Chr II from 560072-560968, Verified ORF [10112005cervSGD.fasta] Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates [Saccharomyces cerevisiae]gi\|3486\|emb\|CAA25065.1\| unnamed protein pr [nrdb_11152004_Chlamydomonas] CDC28 SGDID:S000000364, Chr II from 560072-560968, Verified ORF, "Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] CDC28 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; Cdc28p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] protein CDC28 [nr-271106-contam.fasta] Cell division control protein 28 [nr-271106-contam.fasta] protein kinase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] CDC28 SGDID:S000000364, Chr II from 560078-560974, Genome Release 64-1-1, Verified ORF, "Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates" [yeast-030211-contam.fasta] CDC28 SGDID:S000000364, Chr II from 560078-560974, Genome Release 64-2-1, Verified ORF, "Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1 (CLNs), S and G2/M (CLBs) phase cyclins, which provide substrate specificity; regulates cell cycle and basal transcription, chromosome duplication and segregation, lipid biosynthesis, membrane trafficking, polarized growth, and morphogenesis; abundance increases in DNA replication stress; transcript induction in osmostress involves antisense RNA" [20141208_orf_trans.fasta] TR7509\|c0_g1_i1\|g.5232 ORF TR7509\|c0_g1_i1\|g.5232 TR7509\|c0_g1_i1\|m.5232 type:complete len:299 (+) TR7509\|c0_g1_i1:134-1030(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR1035\|c0_g1_i1\|g.687 ORF TR1035\|c0_g1_i1\|g.687 TR1035\|c0_g1_i1\|m.687 type:complete len:299 (+) TR1035\|c0_g1_i1:156-1052(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YBR160W SGDID:S000000364, Chr II from 560078-560974, Genome Release 64-1-1, Verified ORF, "Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_4750\|g.3425 ORF asmbl_4750\|g.3425 asmbl_4750\|m.3425 type:complete len:299 (+) asmbl_4750:135-1031(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_4740\|g.3640 ORF asmbl_4740\|g.3640 asmbl_4740\|m.3640 type:complete len:299 (+) asmbl_4740:135-1031(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_5074\|g.3083 ORF ID=asmbl_5074\|g.3083 ID=asmbl_5074\|m.3083 type:complete len:299 (+) ID=asmbl_5074:135-1031(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_4745\|g.3020 ORF ID=asmbl_4745\|g.3020 ID=asmbl_4745\|m.3020 type:complete len:299 (+) ID=asmbl_4745:135-1031(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	298 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA] ribosome [IDA]
Biological Process:	G2/M transition of mitotic cell cycle [TAS] transcription [IMP] S phase of mitotic cell cycle [TAS] G1/S transition of mitotic cell cycle [TAS] regulation of meiosis [TAS] protein amino acid phosphorylation [TAS]
Molecular Function:	cyclin-dependent protein kinase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSGELANYKR LEKVGEGTYG VVYKALDLRP GQGQRVVALK KIRLESEDEG VPSTAIREIS  60
   61 LLKELKDDNI VRLYDIVHSD AHKLYLVFEF LDLDLKRYME GIPKDQPLGA DIVKKFMMQL 120
  121 CKGIAYCHSH RILHRDLKPQ NLLINKDGNL KLGDFGLARA FGVPLRAYTH EIVTLWYRAP 180
  181 EVLLGGKQYS TGVDTWSIGC IFAEMCNRKP IFSGDSEIDQ IFKIFRVLGT PNEAIWPDIV 240
  241 YLPDFKPSFP QWRRKDLSQV VPSLDPRGID LLDKLLAYDP INRISARRAA IHPYFQES

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CDC28 CKS1 CLB2 CLB3 CLN1 CLN2 SIC1
View Details	(MIPS) Mewes HW, et al. (2004)		CDC28 CLB1 CLB2 CLB3 CLB4 CLB5 CLB6 CLN1 CLN2 CLN3
View Details	Krogan NJ, et al. (2006)		ATE1 CDC28 CKS1 CLB2 CLB3 CLB4 CLB5 SIC1 SKG3
View Details	Gavin AC, et al. (2006)		CDC28 CKS1 CLN2 CYS4 SIC1
View Details	Gavin AC, et al. (2002)		CDC28 CKS1 CLB3 CLN1 CLN2 DAL7 PCA1 SIC1 SRL3 YPL014W
View Details	Ho Y, et al. (2002)		ATP3 BUR2 CCT2 CCT3 CDC28 CDH1 CEG1 CKA1 CKA2 CKB1 CKB2 CKS1 CLB2 CLB3 CLB5 CLN1 CLN2 CLU1 ECM10 FKH1 FYV8 GCD2 GCD7 GCN3 GSY1 HHF1, HHF2 HTB1 HYP2 MBP1 MET10 MGM101 MPH1 NAP1 NET1 NOP1 PGM2 RPN1 RRP1 SEC2 SIN3 SUI2 SUI3 TCP1 UBP12 UBP15 URE2 YDR170W-A YER138C YGR017W YMR144W
View Details	Qiu et al. (2008)		CDC28 CLB5 CLN1 CLN2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CLB2	No Comments	McCusker D, et al (2007)
View Run	CLB2	No Comments	McCusker D, et al (2007)
View Run	CLB2	No Comments	McCusker D, et al (2007)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample zds1 from august 2005	McCusker D, et al (2007)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC28	MUK1	2	Unpublished Fields Lab Data
View Screen	CLN3	CDC28	2	Unpublished Fields Lab Data
View Screen	CDC28	CLN3	2	Unpublished Fields Lab Data
View Screen	CDC28	CLN3	2	Unpublished Fields Lab Data
View Screen	CDC28	FIN1	2	Unpublished Fields Lab Data
View Screen	CDC28	PCL6	2	Unpublished Fields Lab Data
View Screen	CDC28	CAK1	2	Unpublished Fields Lab Data
View Screen	CDC28	CAK1	2	Unpublished Fields Lab Data
View Screen	CDC28	CLB1	2	Unpublished Fields Lab Data
View Screen	CDC28	PCL7	2	Unpublished Fields Lab Data
View Screen	CDC28	MSA2	2	Unpublished Fields Lab Data
View Screen	CDC28	URB1	2	Unpublished Fields Lab Data
View Screen	CDC28	CLN1	2	Unpublished Fields Lab Data
View Screen	CDC28	MSA2	2	Unpublished Fields Lab Data
View Screen	CDC28	STB1	2	Unpublished Fields Lab Data
View Screen	CDC28	CLN1	2	Unpublished Fields Lab Data
View Screen	CDC28	MSA1	2	Unpublished Fields Lab Data
View Screen	CDC28	CLN2	2	Unpublished Fields Lab Data
View Screen	CDC28	STB1	2	Unpublished Fields Lab Data
View Screen	CLB2	CDC28	2	Unpublished Fields Lab Data
View Screen	CLB5	CDC28	2	Unpublished Fields Lab Data
View Screen	CDC28	YPL014W	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CDC28	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..90]	PSI-BLAST	1220.0	Cyclin-dependent PK, CDK2
2	View Details	[91..172]	PSI-BLAST	1220.0	Cyclin-dependent PK, CDK2
3	View Details	[173..223]	PSI-BLAST	1220.0	Cyclin-dependent PK, CDK2
4	View Details	[224..298]	PSI-BLAST	1220.0	Cyclin-dependent PK, CDK2

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
acetylcholine receptor activator activity	5.9159846946654	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	5.68265749805481	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
cyclin-dependent protein kinase activity	4.62392503670521	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
structural molecule activity	3.82327979736517	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase activity	3.41480681854114	bayes_pls_golite062009
transmembrane receptor protein kinase activity	3.40140621607334	bayes_pls_golite062009
protein tyrosine kinase activity	2.8153155526437	bayes_pls_golite062009
phosphoinositide 3-kinase binding	2.79505438761262	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
transmembrane receptor activity	2.59526003830791	bayes_pls_golite062009
ion transmembrane transporter activity	2.52531786618214	bayes_pls_golite062009
cation transmembrane transporter activity	2.46424348876434	bayes_pls_golite062009
signal transducer activity	2.40586892801446	bayes_pls_golite062009
molecular transducer activity	2.40586892801446	bayes_pls_golite062009
receptor activity	2.39311927684508	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.37122853419919	bayes_pls_golite062009
protein binding	2.276111749106	bayes_pls_golite062009
tubulin binding	2.18641675163967	bayes_pls_golite062009
cytoskeletal protein binding	2.12615078788254	bayes_pls_golite062009
receptor signaling protein activity	2.10379226653669	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
ubiquitin-protein ligase activity	1.94085498993561	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
metal ion transmembrane transporter activity	1.9016235206634	bayes_pls_golite062009
microtubule binding	1.88110801192607	bayes_pls_golite062009
actin binding	1.87817875565699	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.81249386965144	bayes_pls_golite062009
telethonin binding	1.75733591474207	bayes_pls_golite062009
small conjugating protein ligase activity	1.74206248731815	bayes_pls_golite062009
fibroblast growth factor 1 binding	1.66976264488423	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
insulin receptor substrate binding	1.62657794525303	bayes_pls_golite062009
transcription regulator activity	1.56748951434625	bayes_pls_golite062009
growth factor binding	1.53863828216563	bayes_pls_golite062009
nucleic acid binding	1.46547714006072	bayes_pls_golite062009
MAP kinase kinase activity	1.42275431028856	bayes_pls_golite062009
DNA binding	1.35333135345112	bayes_pls_golite062009
ErbB-3 class receptor binding	1.34841233233438	bayes_pls_golite062009
ErbB-2 class receptor binding	1.33825339298024	bayes_pls_golite062009
kinase binding	1.26086833316556	bayes_pls_golite062009
acid-amino acid ligase activity	1.2266791546014	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
transcription elongation regulator activity	1.16995153203536	bayes_pls_golite062009
transcription repressor activity	1.14496167267281	bayes_pls_golite062009
transporter activity	1.11833481501707	bayes_pls_golite062009
non-membrane spanning protein tyrosine kinase activity	1.11664317075762	bayes_pls_golite062009
transcription factor activity	1.10928254946881	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase activity	1.10045286406485	bayes_pls_golite062009
transforming growth factor beta receptor activity	1.10045286406485	bayes_pls_golite062009
protein kinase binding	1.06030596516506	bayes_pls_golite062009
cation channel activity	1.04999722123563	bayes_pls_golite062009
JUN kinase kinase kinase activity	1.00828610859671	bayes_pls_golite062009
transcription activator activity	0.997272622653026	bayes_pls_golite062009
PTB domain binding	0.9900774724568	bayes_pls_golite062009
transmembrane transporter activity	0.97575992765505	bayes_pls_golite062009
alpha-actinin binding	0.877087652539674	bayes_pls_golite062009
insulin-like growth factor receptor binding	0.872782211382796	bayes_pls_golite062009
fibroblast growth factor binding	0.870666814073948	bayes_pls_golite062009
MAP kinase activity	0.81943579965027	bayes_pls_golite062009
actin filament binding	0.794152600084	bayes_pls_golite062009
fibroblast growth factor receptor activity	0.75189004129952	bayes_pls_golite062009
substrate-specific transporter activity	0.705905455086486	bayes_pls_golite062009
hormone receptor binding	0.705067960165986	bayes_pls_golite062009
beta-catenin binding	0.693246095477001	bayes_pls_golite062009
structural constituent of cytoskeleton	0.612883428421652	bayes_pls_golite062009
cyclin-dependent protein kinase activating kinase activity	0.529121666062889	bayes_pls_golite062009
activin binding	0.510364757301021	bayes_pls_golite062009
steroid hormone receptor binding	0.505233004492768	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
enzyme binding	0.46284745266176	bayes_pls_golite062009
protein kinase C activity	0.41587987561486	bayes_pls_golite062009
cyclin-dependent protein kinase regulator activity	0.406297251827056	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.401250804041384	bayes_pls_golite062009
structural constituent of muscle	0.399773397398032	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
structure-specific DNA binding	0.318153468064373	bayes_pls_golite062009
channel activity	0.287189255116606	bayes_pls_golite062009
passive transmembrane transporter activity	0.287189255116606	bayes_pls_golite062009
insulin receptor binding	0.266450215230107	bayes_pls_golite062009
substrate-specific channel activity	0.261869836686301	bayes_pls_golite062009
RNA binding	0.245748631571068	bayes_pls_golite062009
ion channel activity	0.241519346930618	bayes_pls_golite062009
nuclear hormone receptor binding	0.240883589532225	bayes_pls_golite062009
protein complex binding	0.239339759110451	bayes_pls_golite062009
insulin receptor activity	0.237134032032089	bayes_pls_golite062009
SH2 domain binding	0.226760162354251	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	0.149958560815481	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	0.149958560815481	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	0.14842991990838	bayes_pls_golite062009
peptide binding	0.118578378753757	bayes_pls_golite062009
transforming growth factor beta receptor activity, type I	0.0506751594607393	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009
brain-derived neurotrophic factor binding	0.00315481858218991	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2