Protein Overview: SAR1

General Information:
Name(s) found:	SAR1 / YPL218W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6325038\|ref\|NP_015106.1\| GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport; Sar1p [Saccharomyces cerevisiae] [NCBI NR] gi\|4422\|emb\|CAA35978.1\| Sar1p, a GTP-binding protein [Saccharomyces cerevisiae] [NCBI NR] gi\|24158936\|pdb\|1M2O\|D Chain D, Crystal Structure Of The Sec23-Sar1 Complex [NCBI NR] gi\|24158934\|pdb\|1M2O\|B Chain B, Crystal Structure Of The Sec23-Sar1 Complex [NCBI NR] gi\|226734\|prf\|\|1604361A GTP binding protein Sar1p [NCBI NR] gi\|1370452\|emb\|CAA97933.1\| SAR1 [Saccharomyces cerevisiae] [NCBI NR] gi\|134221\|sp\|P20606.1\|SAR1_YEAST RecName: Full=Small COPII coat GTPase SAR1; AltName: Full=GTP-binding protein SAR1; AltName: Full=Secretion-associated RAS-related protein 1 [NCBI NR] pir\|\|A33619 GTP-binding protein SAR1 - yeast (Saccharomyces cerevisiae) [NCBI NR] Small COPII coat GTPase SAR1 OS=Saccharomyces cerevisiae GN=SAR1 PE=1 SV=1 [Swiss-Prot] SAR1 GTP-binding protein of the ARF family [mips] GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport [SGD] (P20606) GTP-binding protein SAR1 [4932.FASTACCERV.txt] Chr XVI from 138697-138724,138864-139408 [hmr_yeast5_DM3-1_pombe.fasta] SAR1, Chr XVI from 138697-138724,138864-139408 [yeast_orfs2] SAR1 SGDID:S0006139, Chr XVI from 138697-138724,138864-139408, Verified ORF [yeastflysequest.fasta] Sar1p [Saccharomyces cerevisiae]gi\|1370452\|emb\|CAA97933.1\| SAR1 [Saccharomyces cerevisiae]gi\|4422\|emb\|CAA35978.1\| Sar1p, a GTP-binding protein [Saccharomyces cerevisiae]gi\|101340\|pir\|\|A33619 GTP-binding protein SAR1 - yeast (Saccharomyces cerevisiae)g [nrdb_11152004_Chlamydomonas] SAR1 SGDID:S000006139, Chr XVI from 138697-138724,138864-139408, Verified ORF, "GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport; Sar1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Sar1p, a GTP-binding protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Chain B, Crystal Structure Of The Sec23-Sar1 Complex [nr-271106-contam.fasta] Chain D, Crystal Structure Of The Sec23-Sar1 Complex [nr-271106-contam.fasta] GTP binding protein Sar1p [nr-271106-contam.fasta] SAR1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GTP-binding protein SAR1 [nr-271106-contam.fasta] [chemostatdb.fasta] Small COPII coat GTPase SAR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SAR1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SAR1 SGDID:S000006139, Chr XVI from 138698-138725,138865-139409, Genome Release 64-1-1, Verified ORF, "GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport" [yeast-030211-contam.fasta] SAR1 SGDID:S000006139, Chr XVI from 138698-138725,138865-139409, Genome Release 64-2-1, Verified ORF, "GTPase, GTP-binding protein of the ARF family; component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport; lowers membrane rigidity helping in vesicle formation" [20141208_orf_trans.fasta] TR3080\|c0_g1_i1\|g.2197 ORF TR3080\|c0_g1_i1\|g.2197 TR3080\|c0_g1_i1\|m.2197 type:complete len:191 (+) TR3080\|c0_g1_i1:90-662(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR4249\|c0_g1_i1\|g.2861 ORF TR4249\|c0_g1_i1\|g.2861 TR4249\|c0_g1_i1\|m.2861 type:complete len:191 (+) TR4249\|c0_g1_i1:71-643(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YPL218W SGDID:S000006139, Chr XVI from 138698-138725,138865-139409, Genome Release 64-1-1, Verified ORF, "GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_3429\|g.2323 ORF asmbl_3429\|g.2323 asmbl_3429\|m.2323 type:complete len:191 (+) asmbl_3429:69-641(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_3389\|g.2247 ORF asmbl_3389\|g.2247 asmbl_3389\|m.2247 type:complete len:191 (+) asmbl_3389:69-641(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_3031\|g.1918 ORF ID=asmbl_3031\|g.1918 ID=asmbl_3031\|m.1918 type:complete len:191 (+) ID=asmbl_3031:69-641(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_3392\|g.2242 ORF ID=asmbl_3392\|g.2242 ID=asmbl_3392\|m.2242 type:complete len:191 (+) ID=asmbl_3392:69-641(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	190 amino acids
Gene Ontology:
Cellular Component:	COPII vesicle coat [TAS]
Biological Process:	ER to Golgi vesicle-mediated transport [TAS]
Molecular Function:	GTPase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAGWDIFGWF RDVLASLGLW NKHGKLLFLG LDNAGKTTLL HMLKNDRLAT LQPTWHPTSE  60
   61 ELAIGNIKFT TFDLGGHIQA RRLWKDYFPE VNGIVFLVDA ADPERFDEAR VELDALFNIA 120
  121 ELKDVPFVIL GNKIDAPNAV SEAELRSALG LLNTTGSQRI EGQRPVEVFM CSVVMRNGYL 180
  181 EAFQWLSQYI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		EMP24 ERP1 ERP2 ERV25 ERV46 SAR1 SEC13 SEC16 SEC23 SEC24 SEC31
View Details	Krogan NJ, et al. (2006)		ARC15 ASR1 CAJ1 CDC7 CRN1 INP52 IRC22 MSK1 PNO1 PST2 RAD34 SAC6 SAR1 SLM1 TCP1 TED1 YPP1
View Details	Gavin AC, et al. (2006)		ADE3 DPS1 FUR1 SAR1 TDH1 YDR341C YNK1
View Details	Ho Y, et al. (2002)		BFR2 BIR1 BNA5 CDC15 CDC7 DUG2 ECM10 GCD11 JIP3 MCX1 NMD3 NUT1 PDC5 PDC6 PST2 RPC19 SAR1 SEC27 STI1 TEM1 THI3 TPS1 UBI4 VAC14 YHR033W YNK1
View Details	Qiu et al. (2008)		APL4 ARF1 ARF2 COP1 EMP24 ERP1 ERP2 ERV25 SAR1 SEC13 SEC16 SEC21 SEC23 SEC24 SEC27 SEC28 SEC31 SEC4 SFB2 SFB3 YOS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	GDI1	SAR1	1	Unpublished Fields Lab Data
View Screen	RAD6	SAR1	1	Unpublished Fields Lab Data
View Screen	CDC23	SAR1	1	Unpublished Fields Lab Data
View Screen	FAR10	SAR1	1	Unpublished Fields Lab Data
View Screen	RAD1	SAR1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..190]	PSI-BLAST	1122.154902	SAR1

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
motor activity	3.3674051903467	bayes_pls_golite062009
transporter activity	3.10463051760206	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.95079405147356	bayes_pls_golite062009
microtubule motor activity	2.9037759597776	bayes_pls_golite062009
pyrophosphatase activity	2.87427509025394	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.85499564954033	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.85178667290149	bayes_pls_golite062009
primary active transmembrane transporter activity	2.61775212528604	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.61467977505707	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.57552435627908	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.57107347424517	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.57054427875021	bayes_pls_golite062009
microfilament motor activity	2.51786963027655	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
ATPase activity, coupled	2.43862163407966	bayes_pls_golite062009
cytoskeletal protein binding	2.4101693596493	bayes_pls_golite062009
ATPase activity	2.39535714892224	bayes_pls_golite062009
actin binding	2.2973522188675	bayes_pls_golite062009
amine transmembrane transporter activity	2.29486577878686	bayes_pls_golite062009
binding	2.21604035604783	bayes_pls_golite062009
substrate-specific transporter activity	1.91091452720653	bayes_pls_golite062009
guanyl nucleotide binding	1.89320355239381	bayes_pls_golite062009
guanyl ribonucleotide binding	1.88721318481223	bayes_pls_golite062009
GTP binding	1.87900088157196	bayes_pls_golite062009
alpha-tubulin binding	1.792591628112	bayes_pls_golite062009
organic acid transmembrane transporter activity	1.77111739879769	bayes_pls_golite062009
carboxylic acid transmembrane transporter activity	1.75695995185278	bayes_pls_golite062009
protein transporter activity	1.71214667007262	bayes_pls_golite062009
GTPase activity	1.6309422514633	bayes_pls_golite062009
tubulin binding	1.62788446088451	bayes_pls_golite062009
hydrolase activity	1.57549510726226	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.56325751172863	bayes_pls_golite062009
nucleic acid binding	1.51655225251251	bayes_pls_golite062009
transcription regulator activity	1.49517431175162	bayes_pls_golite062009
signal recognition particle binding	1.46975774710831	bayes_pls_golite062009
microtubule binding	1.44471873069962	bayes_pls_golite062009
DNA binding	1.28683669571887	bayes_pls_golite062009
actin filament binding	1.28204719195916	bayes_pls_golite062009
protein binding	1.22129154759482	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
ATP binding	0.98146619596929	bayes_pls_golite062009
ion transmembrane transporter activity	0.977237423302079	bayes_pls_golite062009
adenyl ribonucleotide binding	0.949788783884952	bayes_pls_golite062009
RNA helicase activity	0.94708387424268	bayes_pls_golite062009
adenyl nucleotide binding	0.931486373329219	bayes_pls_golite062009
ATP-dependent RNA helicase activity	0.93133090558732	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
actin-dependent ATPase activity	0.92105578610354	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
RNA-dependent ATPase activity	0.91827876091109	bayes_pls_golite062009
kinase activity	0.691112783105676	bayes_pls_golite062009
molecular transducer activity	0.683309012097319	bayes_pls_golite062009
signal transducer activity	0.683309012097319	bayes_pls_golite062009
amino acid transmembrane transporter activity	0.585668369219566	bayes_pls_golite062009
cation transmembrane transporter activity	0.557919489256916	bayes_pls_golite062009
ribonucleoprotein binding	0.53788302029845	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.499834566483656	bayes_pls_golite062009
drug transmembrane transporter activity	0.38711726712063	bayes_pls_golite062009
transition metal ion transmembrane transporter activity	0.305627755818843	bayes_pls_golite062009
plus-end-directed microtubule motor activity	0.267845390391521	bayes_pls_golite062009
protein kinase activity	0.214608777661308	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.213018989352421	bayes_pls_golite062009
structural constituent of ribosome	0.208665086324262	bayes_pls_golite062009
transcription factor activity	0.139974763025221	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.123304946374679	bayes_pls_golite062009
beta-tubulin binding	0.115598037081252	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.0430764033739892	bayes_pls_golite062009
structural constituent of cytoskeleton	0.0111338193708552	bayes_pls_golite062009
dynein binding	0.00615444901942652	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2