Protein Overview: FAA4

General Information:
Name(s) found:	FAA4 / YMR246W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|736312\|emb\|CAA88656.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6323903\|ref\|NP_013974.1\| Long chain fatty acyl-CoA synthetase, regulates protein modification during growth in the presence of ethanol, functions to incorporate palmitic acid into phospholipids and neutral lipids; Faa4p [Saccharomyces cerevisiae] [NCBI NR] gi\|1346423\|sp\|P47912.1\|LCF4_YEAST RecName: Full=Long-chain-fatty-acid--CoA ligase 4; AltName: Full=Long-chain acyl-CoA synthetase 4; AltName: Full=Fatty acid activator 4 [NCBI NR] pir\|\|S56060 long-chain-fatty-acid-CoA ligase (EC 6.2.1.3) FAA4 - yeast (Saccharomyces cerevisiae) [NCBI NR] Long-chain-fatty-acid--CoA ligase 4 OS=Saccharomyces cerevisiae GN=FAA4 PE=1 SV=1 [Swiss-Prot] FAA4 Long-chain-fatty-acid--CoA ligase [mips] Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles [SGD] FAA4, Chr XIII from 759806-761890 [yeast_orfs2] Chr XIII from 759806-761890 [yeastorf3.fasta] (P47912) Long-chain-fatty-acid--CoA ligase 4 (EC 6.2.1.3) (Long-chain acyl-CoA synthetase 4) (Fatty acid activator 4) [4932.FASTACCERV.txt] FAA4 SGDID:S0004860, Chr XIII from 759806-761890, Verified ORF [yeastflysequest.fasta] Faa4p [Saccharomyces cerevisiae]gi\|736312\|emb\|CAA88656.1\| unknown [Saccharomyces cerevisiae]gi\|1346423\|sp\|P47912\|LCF4_YEAST Long-chain-fatty-acid--CoA ligase 4 (Long-chain acyl-CoA synthetase 4) (Fatty acid activator 4)gi\|1084773\|pir\|\|S56060 long-chain [nrdb_11152004_Chlamydomonas] FAA4 SGDID:S000004860, Chr XIII from 759806-761890, Verified ORF, "Long chain fatty acyl-CoA synthetase, regulates protein modification during growth in the presence of ethanol, functions to incorporate palmitic acid into phospholipids and neutral lipids" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Long chain fatty acyl-CoA synthetase, regulates protein modification during growth in the presence of ethanol, functions to incorporate palmitic acid into phospholipids and neutral lipids; Faa4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Long-chain-fatty-acid--CoA ligase 4 (Long-chain acyl-CoA synthetase 4) (Fatty acid activator 4) [nr-271106-contam.fasta] [chemostatdb.fasta] Long-chain-fatty-acid--CoA ligase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FAA4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] FAA4 SGDID:S000004860, Chr XIII from 759807-761891, Genome Release 64-1-1, Verified ORF, "Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles" [yeast-030211-contam.fasta] FAA4 SGDID:S000004860, Chr XIII from 759807-761891, Genome Release 64-2-1, Verified ORF, "Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YMR246W SGDID:S000004860, Chr XIII from 759807-761891, Genome Release 64-1-1, Verified ORF, "Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	694 amino acids
Gene Ontology:
Cellular Component:	lipid particle [IDA] cytoplasm [IDA]
Biological Process:	lipid transport [IGI][IPI] lipid metabolic process [IGI][IMP] N-terminal protein myristoylation [IGI][IMP]
Molecular Function:	long-chain fatty acid-CoA ligase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTEQYSVAVG EAANEHETAP RRNIRVKDQP LIRPINSSAS TLYEFALECF TKGGKRDGMA  60
   61 WRDIIDIHET KKTIVKRVDG KDKPIEKTWL YYELTPYITM TYEEMICVMH DIGRGLIKIG 120
  121 VKPNGENKFH IFASTSHKWM KTFLGCMSQG IPVVTAYDTL GESGLIHSMV ETDSVAIFTD 180
  181 NQLLSKLAVP LKTAKNVKFV IHNEPIDPSD KRQNGKLYKA AKDAVDKIKE VRPDIKIYSF 240
  241 DEIIEIGKKA KDEVELHFPK PEDPACIMYT SGSTGTPKGV VLTHYNIVAG IGGVGHNVIG 300
  301 WIGPTDRIIA FLPLAHIFEL TFEFEAFYWN GILGYANVKT LTPTSTRNCQ GDLMEFKPTV 360
  361 MVGVAAVWET VRKGILAKIN ELPGWSQTLF WTVYALKERN IPCSGLLSGL IFKRIREATG 420
  421 GNLRFILNGG SAISIDAQKF LSNLLCPMLI GYGLTEGVAN ACVLEPEHFD YGIAGDLVGT 480
  481 ITAKLVDVED LGYFAKNNQG ELLFKGAPIC SEYYKNPEET AAAFTDDGWF RTGDIAEWTP 540
  541 KGQVKIIDRK KNLVKTLNGE YIALEKLESI YRSNPYVQNI CVYADENKVK PVGIVVPNLG 600
  601 HLSKLAIELG IMVPGEDVES YIHEKKLQDA VCKDMLSTAK SQGLNGIELL CGIVFFEEEW 660
  661 TPENGLVTSA QKLKRRDILA AVKPDVERVY KENT

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2006)		ACO1 FAA4 PGC1
View Details	Ho Y, et al. (2002)		ACC1 ADR1 AFG2 AHA1 ALB1 ARP2 ARX1 BBC1 BFA1 BGL2 CCT6 CDC15 CDC33 CDC39 CDC53 CLU1 CPR6 CRM1 DSS4 DUG2 DUR1,2 EAP1 ECM29 ECM33 EXG1 FAA1 FAA4 FET3 GAL3 GCD1 GCD11 GCD2 GCD6 GCD7 GCN1 GCN3 GLT1 GSF2 HRT1 HYP2 LIA1 LOS1 MAE1 MDH2 MDN1 MET18 MKT1 MMS1 MYO2 NDE1 NMD5 NOG1 NOP7 NPA3 NUP53 OLA1 PDI1 PFK1 PMA2 PRO3 PSR2 RFC2 RMT2 RNR1 RNR2 RPA190 RPN1 RPN5 RPT4 SAC6 SAP155 SAP185 SAP190 SCW4 SEC4 SEC53 SGD1 SIT4 SNF4 SSD1 SUI3 TAP42 TEM1 TPS1 TRP2 UBI4 UTP5 VAC8 VMA8 VPS13 YAR009C YER138C YGP1 YIL108W YLR035C-A YMR196W YPT1 YTM1
View Details	Qiu et al. (2008)		FAA4 FAT1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	VAM3	sample: hx3	Hao Xu, et al. (2010)
View Run	VAM3	sample: hx4	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX13 - trans-SNARE complex forms but fusion is blocked.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	VAM3	hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	FAA4	Huh WK, et al. (2003)
View Data	FAA4 and ERG6	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..63] [88..100] [267..550]	PSI-BLAST	274.9794	Luciferase
2	View Details	[64..87] [101..266] [551..694]	PSI-BLAST	274.9794	Luciferase

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.94

Protein Overview: FAA4

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: