Protein Overview: RFC4

General Information:
Name(s) found:	RFC4 / YOL094C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|841468\|gb\|AAC49063.1\| Rfc4p [NCBI NR] gi\|730503\|sp\|P40339.1\|RFC4_YEAST RecName: Full=Replication factor C subunit 4; Short=Replication factor C4; AltName: Full=Activator 1 37 kDa subunit [NCBI NR] gi\|6324478\|ref\|NP_014547.1\| Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and e [NCBI NR] pir\|\|A53845 replication factor C chain RFC4 [validated] - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|600464\|emb\|CAA58185.1\| orf 00923 [Saccharomyces cerevisiae] [NCBI NR] gi\|499704\|gb\|AAA34970.1\| 37 kDa subunit [NCBI NR] gi\|1419942\|emb\|CAA99106.1\| RFC4 [Saccharomyces cerevisiae] [NCBI NR] Replication factor C subunit 4 OS=Saccharomyces cerevisiae GN=RFC4 PE=1 SV=1 [Swiss-Prot] RFC4 DNA replication factor C, 37 kDa subunit [mips] Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon [SGD] (P40339) Activator 1 37 kDa subunit (Replication factor C subunit 4) (Replication factor C4) [4932.FASTACCERV.txt] Chr XV from 142554-141583, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 141583-142554, reverse complement [yeastorf3.fasta] RFC4, Chr XV from 141583-142554, reverse complement [yeast_orfs2] RFC4 SGDID:S0005454, Chr XV from 142554-141583, reverse complement, Verified ORF [yeastflysequest.fasta] Rfc4p [Saccharomyces cerevisiae]gi\|600464\|emb\|CAA58185.1\| orf 00923 [Saccharomyces cerevisiae]gi\|1419942\|emb\|CAA99106.1\| RFC4 [Saccharomyces cerevisiae]gi\|841468\|gb\|AAC49063.1\| Rfc4pgi\|626874\|pir\|\|A53845 replication factor C chain RFC4 [validated] - y [nrdb_11152004_Chlamydomonas] RFC4 SGDID:S000005454, Chr XV from 142554-141583, reverse complement, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen ( [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RFC4 SGDID:S000005454, Chr XV from 142555-141584, reverse complement, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen ( [yeast-contam.fasta] Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; Rfc4p [Saccharomyces [nr-271106-contam.fasta] 37 kDa subunit [nr-271106-contam.fasta] Rfc4p [nr-271106-contam.fasta] Replication factor C subunit 4 (Replication factor C4) (Activator 1 37 kDa subunit) [nr-271106-contam.fasta] RFC4 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] orf 00923 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RFC4 SGDID:S000005454, Chr XV from 142555-141584, reverse complement, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Replication factor C subunit 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RFC4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RFC4 SGDID:S000005454, Chr XV from 142555-141584, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" [yeast-030211-contam.fasta] RFC4 SGDID:S000005454, Chr XV from 142555-141584, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia" [20141208_orf_trans.fasta] TR98\|c0_g1_i1\|g.35 ORF TR98\|c0_g1_i1\|g.35 TR98\|c0_g1_i1\|m.35 type:complete len:324 (+) TR98\|c0_g1_i1:122-1093(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YOL094C SGDID:S000005454, Chr XV from 142555-141584, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	323 amino acids
Gene Ontology:
Cellular Component:	DNA replication factor C complex [IDA][ISS]
Biological Process:	sister chromatid cohesion [IPI] leading strand elongation [IDA] mismatch repair [TAS]
Molecular Function:	DNA clamp loader activity [IDA ] purine nucleotide binding [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKTLSLQLP WVEKYRPQVL SDIVGNKETI DRLQQIAKDG NMPHMIISGM PGIGKTTSVH  60
   61 CLAHELLGRS YADGVLELNA SDDRGIDVVR NQIKHFAQKK LHLPPGKHKI VILDEADSMT 120
  121 AGAQQALRRT MELYSNSTRF AFACNQSNKI IEPLQSRCAI LRYSKLSDED VLKRLLQIIK 180
  181 LEDVKYTNDG LEAIIFTAEG DMRQAINNLQ STVAGHGLVN ADNVFKIVDS PHPLIVKKML 240
  241 LASNLEDSIQ ILRTDLWKKG YSSIDIVTTS FRVTKNLAQV KESVRLEMIK EIGLTHMRIL 300
  301 EGVGTYLQLA SMLAKIHKLN NKA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ELG1 RFC1 RFC2 RFC3 RFC4 RFC5
View Details	(MIPS) Mewes HW, et al. (2004)		RFC1 RFC2 RFC3 RFC4 RFC5
View Details	Krogan NJ, et al. (2006)		ELG1 RFC1 RFC2 RFC3 RFC4 RFC5
View Details	Gavin AC, et al. (2006)		ELG1 RFC1 RFC3 RFC4 RFC5
View Details	Gavin AC, et al. (2002)		CTF18 CTF8 DPB2 ELG1 MIS1 OLA1 POL1 POL12 POL2 PRI1 PRI2 RFC1 RFC2 RFC3 RFC4 RFC5 TOP2 USO1 VPS13 YMR181C
View Details	Ho Y, et al. (2002)		ACO1 ADE5,7 ADH2 AHA1 ATP3 BRR2 COP1 DDC1 ELG1 GFA1 GSY1 GUS1 HSP104 MEC3 NUG1 RFC1 RFC2 RFC3 RFC4 RFC5 RNQ1 ROM2 RPN11 RPT3 SAN1 SHM2 SMC3 SUV3 TMA29 UBR1 URA7 VAC8 YCL042W YGL081W YHR020W
View Details	Qiu et al. (2008)		CDC45 DCC1 DPB11 DPB4 MCM3 MCM4 MCM6 MCM7 POL12 POL3 POL30 POL31 POL32 RFA2 RFA3 RFC1 RFC2 RFC3 RFC4 RFC5 RRM3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	RFC4	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..14] [167..230]	PSI-BLAST	785.39794	Replication factor C
2	View Details	[15..166] [231..323]	PSI-BLAST	785.39794	Replication factor C

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Protein Overview: RFC4

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: