Protein Overview: ADE4

General Information:
Name(s) found:	ADE4 / YMR300C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|798942\|emb\|CAA89133.1\| Ade4p [Saccharomyces cerevisiae] [NCBI NR] gi\|6323958\|ref\|NP_014029.1\| Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; Ade4p [Saccharomyces cerevisiae] [NCBI NR] gi\|1346913\|sp\|P04046.2\|PUR1_YEAST RecName: Full=Amidophosphoribosyltransferase; Short=ATase; AltName: Full=Glutamine phosphoribosylpyrophosphate amidotransferase [NCBI NR] pir\|\|S53970 amidophosphoribosyltransferase (EC 2.4.2.14) - yeast (Saccharomyces cerevisiae) [NCBI NR] Amidophosphoribosyltransferase OS=Saccharomyces cerevisiae GN=ADE4 PE=1 SV=2 [Swiss-Prot] ADE4 Amidophosphoribosyltransferase [mips] Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway [SGD] ADE4, Chr XIII from 865559-867091, reverse complement [yeast_orfs2] Chr XIII from 865559-867091, reverse complement [yeastorf3.fasta] Chr XIII from 867091-865559, reverse complement [yeastorf5.fasta] (P04046) Amidophosphoribosyltransferase (EC 2.4.2.14) (Glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) [4932.FASTACCERV.txt] ADE4 SGDID:S0004915, Chr XIII from 867090-865558, reverse complement, Verified ORF [yeastflysequest.fasta] Ade4p [Saccharomyces cerevisiae]gi\|798942\|emb\|CAA89133.1\| Ade4p [Saccharomyces cerevisiae]gi\|1346913\|sp\|P04046\|PUR1_YEAST Amidophosphoribosyltransferase (Glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE)gi\|1076995\|pir\|\|S53970 amidophospho [nrdb_11152004_Chlamydomonas] ADE4 SGDID:S000004915, Chr XIII from 867090-865558, reverse complement, Verified ORF, "Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Amidophosphoribosyltransferase (Glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) [nr-271106-contam.fasta] Ade4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; Ade4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Amidophosphoribosyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ADE4 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ADE4 SGDID:S000004915, Chr XIII from 867091-865559, Genome Release 64-1-1, reverse complement, Verified ORF, "Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway" [yeast-030211-contam.fasta] ADE4 SGDID:S000004915, Chr XIII from 867091-865559, Genome Release 64-2-1, reverse complement, Verified ORF, "Phosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferase" [20141208_orf_trans.fasta] TR7266\|c0_g1_i1\|g.5265 ORF TR7266\|c0_g1_i1\|g.5265 TR7266\|c0_g1_i1\|m.5265 type:complete len:511 (+) TR7266\|c0_g1_i1:185-1717(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YMR300C SGDID:S000004915, Chr XIII from 867091-865559, Genome Release 64-1-1, reverse complement, Verified ORF, "Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_3803\|g.2777 ORF asmbl_3803\|g.2777 asmbl_3803\|m.2777 type:complete len:511 (+) asmbl_3803:144-1676(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_3808\|g.2374 ORF ID=asmbl_3808\|g.2374 ID=asmbl_3808\|m.2374 type:complete len:511 (+) ID=asmbl_3808:144-1676(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	510 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	purine nucleotide biosynthetic process [TAS] 'de novo' IMP biosynthetic process [TAS] purine base metabolic process [IMP][TAS]
Molecular Function:	amidophosphoribosyltransferase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MCGILGIVLA NQTTPVAPEL CDGCIFLQHR GQDAAGIATC GSRGRIYQCK GNGMARDVFT  60
   61 QQRVSGLAGS MGIAHLRYPT AGSSANSEAQ PFYVNSPYGI NLAHNGNLVN TASLKRYMDE 120
  121 DVHRHINTDS DSELLLNIFA AELEKHNKYR VNNEDVFHAL EGVYRLCRGG YACVGLLAGF 180
  181 ALFGFRDPNG IRPLLFGERE NPDGTKDYML ASESVVFKAH NFTKYRDLKP GEAVIIPKNC 240
  241 SKGEPEFKQV VPINSYRPDL FEYVYFARPD SVLDGISVYH TRLAMGSKLA ENILKQLKPE 300
  301 DIDVVIPVPD TARTCALECA NVLGKPYREG FVKNRYVGRT FIMPNQRERV SSVRRKLNPM 360
  361 ESEFKGKKVL IVDDSIVRGT TSKEIVNMAK ESGATKVYFA SAAPAIRYNH IYGIDLTDTK 420
  421 NLIAYNRTDE EVAEVIGCER VIYQSLEDLI DCCKTDKITK FEDGVFTGNY VTGVEDGYIQ 480
  481 ELEEKRESIA NNSSDMKAEV DIGLYNCADY

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2002)		ADE4 ASC1 BFR2 BMS1 BRX1 BUD21 CBF5 CFT2 CKA1 CKA2 CKB1 CKB2 CMS1 CYS3 DBP8 DIA4 DIM1 DIP2 DIS3 ECM16 ECM29 EMG1 ENP1 ENP2 ERO1 ESF1 ESF2 FAP7 FIP1 GCN1 GLC7 HAS1 HCA4 HHF1, HHF2 HSL1 HTA2 IMP3 IMP4 KRE33 KRR1 LCP5 LTV1 MPP10 MRD1 MVD1 NAN1 NOB1 NOC2 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PFS2 PNO1 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 RCL1 REF2 RML2 RNA1 RNA14 ROK1 RPP2B RRP12 RRP7 RRP9 RTG2 SAM1 SCL1 SEC21 SLX9 SOF1 SRO9 TIF1, TIF2 TSR1 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 YOR059C YSH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	ADE4	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..270] [458..510]	PSI-BLAST	1526.0	Glutamine PRPP amidotransferase, C-terminal domain; Glutamine PRPP amidotransferase, N-terminal domain
2	View Details	[271..457]	PSI-BLAST	1526.0	Glutamine PRPP amidotransferase, C-terminal domain; Glutamine PRPP amidotransferase, N-terminal domain

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.61

Protein Overview: ADE4

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: