Protein Overview: CDC16

General Information:
Name(s) found:	CDC16 / YKL022C [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|A27832 cell division control protein CDC16 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6322830\|ref\|NP_012903.1\| Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for [NCBI NR] gi\|486015\|emb\|CAA81857.1\| CDC16 [Saccharomyces cerevisiae] [NCBI NR] gi\|3478\|emb\|CAA29521.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|115908\|sp\|P09798.1\|CDC16_YEAST RecName: Full=Anaphase-promoting complex subunit CDC16; AltName: Full=Cell division control protein 16 [NCBI NR] Anaphase-promoting complex subunit CDC16 OS=Saccharomyces cerevisiae GN=CDC16 PE=1 SV=1 [Swiss-Prot] (P09798) Cell division control protein 16 [Swiss-Prot] CDC16 Subunit of anaphase-promoting complex (cyclosome) [mips] Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation [SGD] CDC16, Chr XI from 394364-396886, reverse complement [yeast_orfs2] Chr XI from 394364-396886, reverse complement [yeastorf3.fasta] Chr XI from 396886-394364, reverse complement [yeastorf5.fasta] CDC16 SGDID:S0001505, Chr XI from 396891-394369, reverse complement, Verified ORF [yeastflysequest.fasta] Cdc16p [Saccharomyces cerevisiae]gi\|486015\|emb\|CAA81857.1\| CDC16 [Saccharomyces cerevisiae]gi\|3478\|emb\|CAA29521.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|82934\|pir\|\|A27832 cell division control protein CDC16 - yeast (Saccharomyces cerevis [nrdb_11152004_Chlamydomonas] CDC16 SGDID:S000001505, Chr XI from 396891-394369, reverse complement, Verified ORF, "Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cycli [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] CDC16 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Anaphase-promoting complex subunit CDC16 (Cell division control protein 16) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; Cdc16p [Saccha [nr-271106-contam.fasta] CDC16 SGDID:S000001505, Chr XI from 396891-394369, reverse complement, Verified ORF, "Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Anaphase-promoting complex subunit CDC16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CDC16 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CDC16 SGDID:S000001505, Chr XI from 397248-394726, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation" [yeast-030211-contam.fasta] CDC16 SGDID:S000001505, Chr XI from 397248-394726, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of the anaphase-promoting complex/cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; relocalizes to the cytosol in response to hypoxia" [20141208_orf_trans.fasta] YKL022C SGDID:S000001505, Chr XI from 397248-394726, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	840 amino acids
Gene Ontology:
Cellular Component:	anaphase-promoting complex [TAS]
Biological Process:	mitotic spindle elongation [TAS] cyclin catabolic process [TAS] mitotic sister chromatid segregation [TAS] anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA][IPI] mitotic metaphase/anaphase transition [TAS] protein ubiquitination [IPI]
Molecular Function:	ubiquitin-protein ligase activity [TAS] protein binding [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKFCLYCCHC YIVICGKATH YYKSSKATSN LKSSNRVLMR NPMSPSEQHS QHNSTLAASP  60
   61 FVSNVSAART QQSLPTDAQN DRLQQPWNRT NTATSPYQSL ANSPLIQKLQ ANIMTPHQPS 120
  121 ANSNSNSNSI TGNVVNDNNL LASMSKNSMF GSTIPSTLRK VSLQREYKDS VDGVVRDEDN 180
  181 DEDVHNNGDA AANANNDRES KLGHNGPLTT TTLTTTTTAT QLDVSELSAI ERLRLWRFDA 240
  241 LMQHMYRTAE YIADKVYNIS NDPDDAFWLG QVYYNNNQYV RAVELITRNN LDGVNILCRY 300
  301 LLGLSFVKLQ RFDDALDVIG EYNPFSEDPS TTAANTMSNN GNNSNTSQPV TDGGIKMESS 360
  361 LCFLRGKIYF AQNNFNKARD AFREAILVDI KNFEAFEMLL SKNLLTPQEE WDLFDSLDFK 420
  421 EFGEDKEIMK NLYKINLSKY INTEDITKSN EILAKDYKLA DNVDVVRSKV DICYTQCKFN 480
  481 ECLELCETVL ENDEFNTNIL PAYIGCLYEL SNKNKLFLLS HRLAETFPKS AITWFSVATY 540
  541 YMSLDRISEA QKYYSKSSIL DPSFAAAWLG FAHTYALEGE QDQALTAYST ASRFFPGMHL 600
  601 PKLFLGMQFM AMNSLNLAES YFVLAYDICP NDPLVLNEMG VMYFKKNEFV KAKKYLKKAL 660
  661 EVVKDLDPSS RTTISIQLNL GHTYRKLNEN EIAIKCFRCV LEKNDKNSEI HCSLGYLYLK 720
  721 TKKLQKAIDH LHKSLYLKPN NSSATALLKN ALELNVTLSL DASHPLIDKS NLMSQASKDK 780
  781 ASLNKKRSSL TYDPVNMAKR LRTQKEIFDQ NNKALRKGGH DSKTGSNNAD DDFDADMELE 840
  841

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		APC1 APC2 APC4 CDC16 CDC23 CDC27
View Details	(MIPS) Mewes HW, et al. (2004)		APC1 APC11 APC2 APC4 APC5 APC9 CDC16 CDC23 CDC26 CDC27 DOC1
View Details	Krogan NJ, et al. (2006)		APC1 APC2 APC4 APC5 CDC16 CDC23 CDC26 CDC27 DOC1 FMP52 MND2 SSP2
View Details	Gavin AC, et al. (2006)		APC1 APC2 APC4 APC5 APC9 CDC16 CDC23 CDC27
View Details	Gavin AC, et al. (2002)		ACT1 APC1 APC2 CDC16 CDC23 CDC27
View Details	Ho Y, et al. (2002)		ADH2 AHP1 CDC16 CDC33 ERB1 ERG20 FPR3 HHF1, HHF2 KRE33 KRI1 LOC1 MES1 MKT1 MMS4 MRE11 MUS81 NHP2 NOP12 NOP2 PWP1 RAD50 RAD53 RPC10 RPF2 SEC23 TAF14 UBI4 UTP22 XRS2 YHB1 YRA1
View Details	Qiu et al. (2008)		APC1 APC11 APC2 APC4 APC5 APC9 BUL2 CDC16 CDC23 CDC26 CDC27 DOC1 MND2 RSP5 SWM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC16	YGR228W	1	Unpublished Fields Lab Data
View Screen	CDC16	YHR162W	1	Unpublished Fields Lab Data
View Screen	CDC16	BUD19	1	Unpublished Fields Lab Data
View Screen	CDC16	HOM6	1	Unpublished Fields Lab Data
View Screen	CDC16	DID4	1	Unpublished Fields Lab Data
View Screen	CDC16	COX17	1	Unpublished Fields Lab Data
View Screen	CDC16	SEC61	1	Unpublished Fields Lab Data
View Screen	LIA1	CDC16	1	Unpublished Fields Lab Data
View Screen	CDC16	NAM2	1	Unpublished Fields Lab Data
View Screen	CDC16	VID24	1	Unpublished Fields Lab Data
View Screen	CDC16	RAP1	1	Unpublished Fields Lab Data
View Screen	CDC16	YBR137W	1	Unpublished Fields Lab Data
View Screen	CDC16	YPR147C	1	Unpublished Fields Lab Data
View Screen	CDC16	HSP30	1	Unpublished Fields Lab Data
View Screen	CDC16	MLC2	1	Unpublished Fields Lab Data
View Screen	CDC16	THI3	1	Unpublished Fields Lab Data
View Screen	RPC19	CDC16	1	Unpublished Fields Lab Data
View Screen	CDC16	ACK1	1	Unpublished Fields Lab Data
View Screen	YIF1	CDC16	1	Unpublished Fields Lab Data
View Screen	CDC16	ATC1	1	Unpublished Fields Lab Data
View Screen	CDC16	TPA1	1	Unpublished Fields Lab Data
View Screen	CDC16	OKP1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	CDC16	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..92]	PSI-BLAST	13.045757	Hop
2	View Details	[93..206]	deduced	N/A	No confident structure predictions are available.
3	View Details	[207..302]	PSI-BLAST	3.30103	Transcription factor MalT domain III
4	View Details	[303..418]	PSI-BLAST	3.30103	Transcription factor MalT domain III
5	View Details	[419..579]	PSI-BLAST	41.522879	Peroxin pex5 (peroxisomal targeting signal 1 (PTS1) receptor)
6	View Details	[580..647]	PSI-BLAST	41.522879	Peroxin pex5 (peroxisomal targeting signal 1 (PTS1) receptor)
7	View Details	[648..788]	PSI-BLAST	41.522879	Peroxin pex5 (peroxisomal targeting signal 1 (PTS1) receptor)
8	View Details	[789..840]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.94

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2