Abstract
Entry into anaphase and proteolysis of B-type cyclins depend on a complex containing the tetratricopeptide repeat proteins Cdc16p, Cdc23p, and Cdc27p. This particle, called the anaphase-promoting complex (APC) or cyclosome, functions as a cell cycle-regulated ubiquitin-protein ligase. Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the APC1 gene, is conserved between fungi and vertebrates and shows similarity to BIMEp from Aspergillus nidulans. A small heat-inducible subunit is encoded by the CDC26 gene. The yeast APC is a 36S particle that contains at least seven different proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anaphase*
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Cell Cycle Proteins / analysis
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Cell Cycle Proteins / chemistry*
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism
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Cell Division
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Centrifugation, Density Gradient
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Cyclins / metabolism
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Fluorescent Antibody Technique, Indirect
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Fungal Proteins / analysis
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Fungal Proteins / chemistry*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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G1 Phase
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Genes, Fungal
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Ligases / chemistry*
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Ligases / metabolism
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Mitosis*
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Saccharomyces cerevisiae / chemistry*
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics
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Ubiquitin-Protein Ligases
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Ubiquitins / metabolism
Substances
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BIME protein, Emericella nidulans
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Cell Cycle Proteins
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Cyclins
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Fungal Proteins
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Ubiquitins
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Ubiquitin-Protein Ligases
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Ligases