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Protein Overview: RAD53

General Information:
Name(s) found: RAD53 / YPL153C [SGD]
Description(s) found:
Found 29 descriptions.
SHOW ALL
  • Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 821 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    Biological Process: DNA replication initiation [IMPLink to PubMed article]
    DNA repair [IMPLink to PubMed article]
    deoxyribonucleoside triphosphate biosynthetic process [IMPLink to PubMed article]
    nucleobase, nucleoside, nucleotide and nucleic acid metabolic process [IGILink to PubMed article]
    Molecular Function: DNA replication origin binding [IDALink to PubMed article]
    protein serine/threonine/tyrosine kinase activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MENITQPTQQ STQATQRFLI EKFSQEQIGE NIVCRVICTT GQIPIRDLSA DISQVLKEKR  60
   61 SIKKVWTFGR NPACDYHLGN ISRLSNKHFQ ILLGEDGNLL LNDISTNGTW LNGQKVEKNS 120
  121 NQLLSQGDEI TVGVGVESDI LSLVIFINDK FKQCLEQNKV DRIRSNLKNT SKIASPGLTS 180
  181 STASSMVANK TGIFKDFSII DEVVGQGAFA TVKKAIERTT GKTFAVKIIS KRKVIGNMDG 240
  241 VTRELEVLQK LNHPRIVRLK GFYEDTESYY MVMEFVSGGD LMDFVAAHGA VGEDAGREIS 300
  301 RQILTAIKYI HSMGISHRDL KPDNILIEQD DPVLVKITDF GLAKVQGNGS FMKTFCGTLA 360
  361 YVAPEVIRGK DTSVSPDEYE ERNEYSSLVD MWSMGCLVYV ILTGHLPFSG STQDQLYKQI 420
  421 GRGSYHEGPL KDFRISEEAR DFIDSLLQVD PNNRSTAAKA LNHPWIKMSP LGSQSYGDFS 480
  481 QISLSQSLSQ QKLLENMDDA QYEFVKAQRK LQMEQQLQEQ DQEDQDGKIQ GFKIPAHAPI 540
  541 RYTQPKSIEA ETREQKLLHS NNTENVKSSK KKGNGRFLTL KPLPDSIIQE SLEIQQGVNP 600
  601 FFIGRSEDCN CKIEDNRLSR VHCFIFKKRH AVGKSMYESP AQGLDDIWYC HTGTNVSYLN 660
  661 NNRMIQGTKF LLQDGDEIKI IWDKNNKFVI GFKVEINDTT GLFNEGLGML QEQRVVLKQT 720
  721 AEEKDLVKKL TQMMAAQRAN QPSASSSSMS AKKPPVSDTN NNGNNSVLND LVESPINANT 780
  781 GNILKRIHSV SLSQSQIDPS KKVKRAKLDQ TSKGPENLQF S

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ASF1 HIR2 HIR3 HPC2 RAD53
    View Details Krogan NJ, et al. (2006) CBC2 KAP95 NUP1 NUP2 NUP60 OGG1 PCT1 RAD53 SRP1 STO1 ULP1 URB2 YRA2
    View Details Gavin AC, et al. (2006) HIR3 MAM33 RAD53
    View Details Gavin AC, et al. (2002) ASF1 HIR3 MAM33 RAD53 RNR2 RNR4
    View Details Ho Y, et al. (2002) ADH2 ASF1 CDC13 CDC16 CDC33 DUN1 EDE1 ERB1 FPR3 GID8 HHF1, HHF2 HTA2 IPP1 KAP95 KRE33 KRI1 LOC1 MDH1 MES1 MKT1 MMS4 MUS81 NHP2 NOP12 NOP2 PAA1 PTC2 PWP1 RAD53 RPC10 RPF2 SEC23 SMC3 SRP1 SWI4 TAF14 TBF1 UBI4 UTP22 YHB1 YRA1 YTA7

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run ASF1 sample 3121 run on DecaLCQ: searched for phosphorylation Green EM, et al (2005)
    View Run ASF1 3906: TAP-tagged Green EM, et al (2005)
    View Run ASF1 3908: TAP-tagged, includes hir2(delta) in background Green EM, et al (2005)
    View Run ASF1 3912: TAP-tagged, strain background includes hpc2(delta) Green EM, et al (2005)
    View Run ASF1 sample 3829 Green EM, et al (2005)
    View Run ASF1 3829 - low filter Green EM, et al (2005)
    View Run ASF1 sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant) Green EM, et al (2005)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen RAD53 SPT21 2 Unpublished Fields Lab Data
    View Screen RAD53 PSY2 2 Unpublished Fields Lab Data
    View Screen RAD9 RAD53 2 Unpublished Fields Lab Data
    View Screen RAD53 FOB1 2 Unpublished Fields Lab Data
    View Screen RAD53 CBF1 2 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RAD53 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..165] PSI-BLAST 904.325697 Phosphotyrosine binding domain of Rad53
    2 View Details [166..278]
    [491..534]    		 PSI-BLAST 622.457575 Calmodulin-dependent protein kinase
    3 View Details [279..490] PSI-BLAST 622.457575 Calmodulin-dependent protein kinase
    4 View Details [535..753] PSI-BLAST 1080.0 Phosphotyrosine binding domain of Rad53
    5 View Details [754..821] deduced N/A No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • binding
    		•  3.6561903894619 bayes_pls_golite062009
  • transcription regulator activity
    		•  3.23926962992034 bayes_pls_golite062009
  • DNA binding
    		•  3.12131448113144 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.91028042727128 bayes_pls_golite062009
  • transforming growth factor beta receptor, cytoplasmic mediator activity
    		•  2.82706319058551 bayes_pls_golite062009
  • histone binding
    		•  2.79033314021304 bayes_pls_golite062009
  • protein binding
    		•  2.39508335485646 bayes_pls_golite062009
  • receptor signaling protein activity
    		•  2.25132862320271 bayes_pls_golite062009
  • transmembrane receptor protein serine/threonine kinase signaling protein activity
    		•  2.10556565012417 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.95921851331594 bayes_pls_golite062009
  • transcription factor activity
    		•  1.89464382469326 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  1.88096476099716 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  1.84746485315093 bayes_pls_golite062009
  • kinase activity
    		•  1.82476774968305 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  1.78280248668052 bayes_pls_golite062009
  • DNA replication origin binding
    		•  1.78246425241581 bayes_pls_golite062009
  • protein kinase activity
    		•  1.73373132141588 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  1.66058356077795 bayes_pls_golite062009
  • SMAD binding
    		•  1.6101563888469 bayes_pls_golite062009
  • transcription repressor activity
    		•  1.60737837207077 bayes_pls_golite062009
  • transcription activator activity
    		•  1.53960544319629 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  1.48483625404671 bayes_pls_golite062009
  • transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
    		•  1.44498281734982 bayes_pls_golite062009
  • type I transforming growth factor beta receptor binding
    		•  1.3333129315031 bayes_pls_golite062009
  • transferase activity
    		•  1.29860325699399 bayes_pls_golite062009
  • protein serine/threonine kinase activity
    		•  1.1868962075754 bayes_pls_golite062009
  • promoter binding
    		•  1.15188009117562 bayes_pls_golite062009
  • transcription factor binding
    		•  0.93544177518754 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  0.9108432673413 bayes_pls_golite062009
  • ubiquitin-protein ligase activity
    		•  0.90981150861416 bayes_pls_golite062009
  • molecular transducer activity
    		•  0.767071864136971 bayes_pls_golite062009
  • signal transducer activity
    		•  0.767071864136971 bayes_pls_golite062009
  • R-SMAD binding
    		•  0.70816286022176 bayes_pls_golite062009
  • chromatin binding
    		•  0.62935034847038 bayes_pls_golite062009
  • ligase activity, forming carbon-nitrogen bonds
    		•  0.447699674047884 bayes_pls_golite062009
  • co-SMAD binding
    		•  0.325865547214071 bayes_pls_golite062009
  • damaged DNA binding
    		•  0.279328480488455 bayes_pls_golite062009
  • receptor serine/threonine kinase binding
    		•  0.270284689687439 bayes_pls_golite062009
  • specific transcriptional repressor activity
    		•  0.0595316953833198 bayes_pls_golite062009
  • protein dimerization activity
    		•  0.0504553861724597 bayes_pls_golite062009
    2 No functions predicted.
    3
    Term Confidence Notes
  • binding
    		•  3.6561903894619 bayes_pls_golite062009
  • transcription regulator activity
    		•  3.23926962992034 bayes_pls_golite062009
  • DNA binding
    		•  3.12131448113144 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.91028042727128 bayes_pls_golite062009
  • transforming growth factor beta receptor, cytoplasmic mediator activity
    		•  2.82706319058551 bayes_pls_golite062009
  • histone binding
    		•  2.79033314021304 bayes_pls_golite062009
  • protein binding
    		•  2.39508335485646 bayes_pls_golite062009
  • receptor signaling protein activity
    		•  2.25132862320271 bayes_pls_golite062009
  • transmembrane receptor protein serine/threonine kinase signaling protein activity
    		•  2.10556565012417 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.95921851331594 bayes_pls_golite062009
  • transcription factor activity
    		•  1.89464382469326 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  1.88096476099716 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  1.84746485315093 bayes_pls_golite062009
  • kinase activity
    		•  1.82476774968305 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  1.78280248668052 bayes_pls_golite062009
  • DNA replication origin binding
    		•  1.78246425241581 bayes_pls_golite062009
  • protein kinase activity
    		•  1.73373132141588 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  1.66058356077795 bayes_pls_golite062009
  • SMAD binding
    		•  1.6101563888469 bayes_pls_golite062009
  • transcription repressor activity
    		•  1.60737837207077 bayes_pls_golite062009
  • transcription activator activity
    		•  1.53960544319629 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  1.48483625404671 bayes_pls_golite062009
  • transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity
    		•  1.44498281734982 bayes_pls_golite062009
  • type I transforming growth factor beta receptor binding
    		•  1.3333129315031 bayes_pls_golite062009
  • transferase activity
    		•  1.29860325699399 bayes_pls_golite062009
  • protein serine/threonine kinase activity
    		•  1.1868962075754 bayes_pls_golite062009
  • promoter binding
    		•  1.15188009117562 bayes_pls_golite062009
  • transcription factor binding
    		•  0.93544177518754 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  0.9108432673413 bayes_pls_golite062009
  • ubiquitin-protein ligase activity
    		•  0.90981150861416 bayes_pls_golite062009
  • molecular transducer activity
    		•  0.767071864136971 bayes_pls_golite062009
  • signal transducer activity
    		•  0.767071864136971 bayes_pls_golite062009
  • R-SMAD binding
    		•  0.70816286022176 bayes_pls_golite062009
  • chromatin binding
    		•  0.62935034847038 bayes_pls_golite062009
  • ligase activity, forming carbon-nitrogen bonds
    		•  0.447699674047884 bayes_pls_golite062009
  • co-SMAD binding
    		•  0.325865547214071 bayes_pls_golite062009
  • damaged DNA binding
    		•  0.279328480488455 bayes_pls_golite062009
  • receptor serine/threonine kinase binding
    		•  0.270284689687439 bayes_pls_golite062009
  • specific transcriptional repressor activity
    		•  0.0595316953833198 bayes_pls_golite062009
  • protein dimerization activity
    		•  0.0504553861724597 bayes_pls_golite062009
    4 No functions predicted.
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.95

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle