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View Structure Prediction Details

Protein: RAD53
Organism: Saccharomyces cerevisiae
Length: 821 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for RAD53.

Description E-value Query
Range		 Subject
Range
RAD53 - Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autopho...
RAD53_YEAST - Serine/threonine-protein kinase RAD53 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RA...
 0.0 [1..821] [1..821]
gi|16215467 - gi|16215467|emb|CAC82998.1| calcium-dependent protein kinase 2 [Nicotiana tabacum]
 0.0 [136..693] [55..576]
gi|15289758 - gi|15289758|dbj|BAB63463.1| calcium dependent protein kinase [Solanum tuberosum]
 0.0 [125..693] [40..573]
gi|20453015 - gi|20453015|gb|AAL68972.1| calmodulin-like-domain protein kinase CPK2 [Cucurbita maxima]
 0.0 [117..669] [16..535]
gi|16215475 - gi|16215475|emb|CAC83000.1| calcium-dependent protein kinase 2 [Nicotiana benthamiana]
 0.0 [136..693] [55..576]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
 0.0 [181..669] [7..464]
gi|14029712 - gi|14029712|gb|AAK52801.1|AF363784_1 calcium-dependent protein kinase CDPK1 [Lycopersicon esculentum...
 0.0 [160..668] [35..513]
CDPK6_ARATH - Calcium-dependent protein kinase 6 OS=Arabidopsis thaliana GN=CPK6 PE=1 SV=1
 0.0 [134..669] [19..525]

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Predicted Domain #1
Region A:
Residues: [1-165] 
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MENITQPTQQ STQATQRFLI EKFSQEQIGE NIVCRVICTT GQIPIRDLSA DISQVLKEKR  60
   61 SIKKVWTFGR NPACDYHLGN ISRLSNKHFQ ILLGEDGNLL LNDISTNGTW LNGQKVEKNS 120
  121 NQLLSQGDEI TVGVGVESDI LSLVIFINDK FKQCLEQNKV DRIRS

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 904.325697
Match: 1g3gA_
Description: Phosphotyrosine binding domain of Rad53
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
binding 3.6561903894619 bayes_pls_golite062009
transcription regulator activity 3.23926962992034 bayes_pls_golite062009
DNA binding 3.12131448113144 bayes_pls_golite062009
nucleic acid binding 2.91028042727128 bayes_pls_golite062009
transforming growth factor beta receptor, cytoplasmic mediator activity 2.82706319058551 bayes_pls_golite062009
histone binding 2.79033314021304 bayes_pls_golite062009
protein binding 2.39508335485646 bayes_pls_golite062009
receptor signaling protein activity 2.25132862320271 bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase signaling protein activity 2.10556565012417 bayes_pls_golite062009
structure-specific DNA binding 1.95921851331594 bayes_pls_golite062009
transcription factor activity 1.89464382469326 bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor 1.88096476099716 bayes_pls_golite062009
double-stranded DNA binding 1.84746485315093 bayes_pls_golite062009
kinase activity 1.82476774968305 bayes_pls_golite062009
sequence-specific DNA binding 1.78280248668052 bayes_pls_golite062009
DNA replication origin binding 1.78246425241581 bayes_pls_golite062009
protein kinase activity 1.73373132141588 bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups 1.66058356077795 bayes_pls_golite062009
SMAD binding 1.6101563888469 bayes_pls_golite062009
transcription repressor activity 1.60737837207077 bayes_pls_golite062009
transcription activator activity 1.53960544319629 bayes_pls_golite062009
RNA polymerase II transcription factor activity 1.48483625404671 bayes_pls_golite062009
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity 1.44498281734982 bayes_pls_golite062009
type I transforming growth factor beta receptor binding 1.3333129315031 bayes_pls_golite062009
transferase activity 1.29860325699399 bayes_pls_golite062009
protein serine/threonine kinase activity 1.1868962075754 bayes_pls_golite062009
promoter binding 1.15188009117562 bayes_pls_golite062009
transcription factor binding 0.93544177518754 bayes_pls_golite062009
small conjugating protein ligase activity 0.9108432673413 bayes_pls_golite062009
ubiquitin-protein ligase activity 0.90981150861416 bayes_pls_golite062009
molecular transducer activity 0.767071864136971 bayes_pls_golite062009
signal transducer activity 0.767071864136971 bayes_pls_golite062009
R-SMAD binding 0.70816286022176 bayes_pls_golite062009
chromatin binding 0.62935034847038 bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds 0.447699674047884 bayes_pls_golite062009
co-SMAD binding 0.325865547214071 bayes_pls_golite062009
damaged DNA binding 0.279328480488455 bayes_pls_golite062009
receptor serine/threonine kinase binding 0.270284689687439 bayes_pls_golite062009
specific transcriptional repressor activity 0.0595316953833198 bayes_pls_golite062009
protein dimerization activity 0.0504553861724597 bayes_pls_golite062009

Predicted Domain #2
Region A:
Residues: [166-278] 
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NLKNTSKIAS PGLTSSTASS MVANKTGIFK DFSIIDEVVG QGAFATVKKA IERTTGKTFA  60
   61 VKIISKRKVI GNMDGVTREL EVLQKLNHPR IVRLKGFYED TESYYMVMEF VSG

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [491-534] 
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 QKLLENMDDA QYEFVKAQRK LQMEQQLQEQ DQEDQDGKIQ GFKI

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 622.457575
Match: 1a06__
Description: Calmodulin-dependent protein kinase
Matching Structure (courtesy of the PDB):

Predicted Domain #3
Region A:
Residues: [279-490] 
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 GDLMDFVAAH GAVGEDAGRE ISRQILTAIK YIHSMGISHR DLKPDNILIE QDDPVLVKIT  60
   61 DFGLAKVQGN GSFMKTFCGT LAYVAPEVIR GKDTSVSPDE YEERNEYSSL VDMWSMGCLV 120
  121 YVILTGHLPF SGSTQDQLYK QIGRGSYHEG PLKDFRISEE ARDFIDSLLQ VDPNNRSTAA 180
  181 KALNHPWIKM SPLGSQSYGD FSQISLSQSL SQ

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 622.457575
Match: 1a06__
Description: Calmodulin-dependent protein kinase
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
binding 3.6561903894619 bayes_pls_golite062009
transcription regulator activity 3.23926962992034 bayes_pls_golite062009
DNA binding 3.12131448113144 bayes_pls_golite062009
nucleic acid binding 2.91028042727128 bayes_pls_golite062009
transforming growth factor beta receptor, cytoplasmic mediator activity 2.82706319058551 bayes_pls_golite062009
histone binding 2.79033314021304 bayes_pls_golite062009
protein binding 2.39508335485646 bayes_pls_golite062009
receptor signaling protein activity 2.25132862320271 bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase signaling protein activity 2.10556565012417 bayes_pls_golite062009
structure-specific DNA binding 1.95921851331594 bayes_pls_golite062009
transcription factor activity 1.89464382469326 bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor 1.88096476099716 bayes_pls_golite062009
double-stranded DNA binding 1.84746485315093 bayes_pls_golite062009
kinase activity 1.82476774968305 bayes_pls_golite062009
sequence-specific DNA binding 1.78280248668052 bayes_pls_golite062009
DNA replication origin binding 1.78246425241581 bayes_pls_golite062009
protein kinase activity 1.73373132141588 bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups 1.66058356077795 bayes_pls_golite062009
SMAD binding 1.6101563888469 bayes_pls_golite062009
transcription repressor activity 1.60737837207077 bayes_pls_golite062009
transcription activator activity 1.53960544319629 bayes_pls_golite062009
RNA polymerase II transcription factor activity 1.48483625404671 bayes_pls_golite062009
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity 1.44498281734982 bayes_pls_golite062009
type I transforming growth factor beta receptor binding 1.3333129315031 bayes_pls_golite062009
transferase activity 1.29860325699399 bayes_pls_golite062009
protein serine/threonine kinase activity 1.1868962075754 bayes_pls_golite062009
promoter binding 1.15188009117562 bayes_pls_golite062009
transcription factor binding 0.93544177518754 bayes_pls_golite062009
small conjugating protein ligase activity 0.9108432673413 bayes_pls_golite062009
ubiquitin-protein ligase activity 0.90981150861416 bayes_pls_golite062009
molecular transducer activity 0.767071864136971 bayes_pls_golite062009
signal transducer activity 0.767071864136971 bayes_pls_golite062009
R-SMAD binding 0.70816286022176 bayes_pls_golite062009
chromatin binding 0.62935034847038 bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds 0.447699674047884 bayes_pls_golite062009
co-SMAD binding 0.325865547214071 bayes_pls_golite062009
damaged DNA binding 0.279328480488455 bayes_pls_golite062009
receptor serine/threonine kinase binding 0.270284689687439 bayes_pls_golite062009
specific transcriptional repressor activity 0.0595316953833198 bayes_pls_golite062009
protein dimerization activity 0.0504553861724597 bayes_pls_golite062009

Predicted Domain #4
Region A:
Residues: [535-753] 
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PAHAPIRYTQ PKSIEAETRE QKLLHSNNTE NVKSSKKKGN GRFLTLKPLP DSIIQESLEI  60
   61 QQGVNPFFIG RSEDCNCKIE DNRLSRVHCF IFKKRHAVGK SMYESPAQGL DDIWYCHTGT 120
  121 NVSYLNNNRM IQGTKFLLQD GDEIKIIWDK NNKFVIGFKV EINDTTGLFN EGLGMLQEQR 180
  181 VVLKQTAEEK DLVKKLTQMM AAQRANQPSA SSSSMSAKK

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 1080.0
Match: 1qu5A_
Description: Phosphotyrosine binding domain of Rad53
Matching Structure (courtesy of the PDB):

Predicted Domain #5
Region A:
Residues: [754-821] 
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PPVSDTNNNG NNSVLNDLVE SPINANTGNI LKRIHSVSLS QSQIDPSKKV KRAKLDQTSK  60
   61 GPENLQFS

[Run NCBI BLAST on this sequence.]

Detection Method: deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle