Protein Overview: AVO1

General Information:
Name(s) found:	AVO1 / YOL078W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324494\|ref\|NP_014563.1\| Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth; Avo1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S66771 hypothetical protein YOL078w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|15214401\|sp\|Q08236.1\|AVO1_YEAST RecName: Full=Target of rapamycin complex 2 subunit AVO1; Short=TORC2 subunit AVO1; AltName: Full=Adheres voraciously to TOR2 protein 1 [NCBI NR] gi\|1419911\|emb\|CAA99089.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] (Q08236) Hypothetical 131.4 kDa protein in REX4-ATP19 intergenic region [Swiss-Prot] (Q08236) Uncharacterized protein YOL078W [Swiss-Prot] Target of rapamycin complex 2 subunit AVO1 OS=Saccharomyces cerevisiae GN=AVO1 PE=1 SV=1 [Swiss-Prot] AVO1 Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth [mips] Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth [SGD] Chr XV from 181681-185211 [hmr_yeast5_DM3-1_pombe.fasta] YOL078W, Chr XV from 181681-185211 [yeast_orfs2] AVO1 SGDID:S0005438, Chr XV from 181681-185211, Verified ORF [yeastflysequest.fasta] Avo1p [Saccharomyces cerevisiae]gi\|1419911\|emb\|CAA99089.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|2132010\|pir\|\|S66771 hypothetical protein YOL078w - yeast (Saccharomyces cerevisiae)gi\|15214401\|sp\|Q08236\|YOH8_YEAST Hypothetical 131.4 kDa p [nrdb_11152004_Chlamydomonas] AVO1 SGDID:S000005438, Chr XV from 181681-185211, Verified ORF, "Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] AVO1 SGDID:S000005438, Chr XV from 181682-185212, Verified ORF, "Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth" [yeast-contam.fasta] Uncharacterized protein YOL078W [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth; Avo1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Target of rapamycin complex 2 subunit AVO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=AVO1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] AVO1 SGDID:S000005438, Chr XV from 181682-185212, Genome Release 64-1-1, Verified ORF, "Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth" [yeast-030211-contam.fasta] AVO1 SGDID:S000005438, Chr XV from 181682-185212, Genome Release 64-2-1, Verified ORF, "Component of a membrane-bound complex containing the Tor2p kinase; contains Tor2p kinase and other proteins; may have a role in regulation of cell growth" [20141208_orf_trans.fasta] YOL078W SGDID:S000005438, Chr XV from 181682-185212, Genome Release 64-1-1, Verified ORF, "Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1176 amino acids
Gene Ontology:
Cellular Component:	TORC2 complex [IPI] cytoplasm [IPI]
Biological Process:	fungal-type cell wall organization [IPI] negative regulation of Ras protein signal transduction [ISS] regulation of cell growth [IPI] establishment or maintenance of actin cytoskeleton polarity [IMP][IPI]
Molecular Function:	Ras GTPase binding [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDTVTVLNEL RAQFLRVCPE KDQMKRIIKP YIPVDEFNTE QCLDSSIREL YMNSDGVSLL  60
   61 PELESPPVSK DFMENYASLG KMRIMRENEG QKGKANQNLI GAEKTERDEE ETRNLQDKSA 120
  121 KNTLIVEENG TLRYNPLNSS ASNSLLNDDD HTSGKHHKTS SKEDSYLNSS MEMQKKSSKR 180
  181 SSLPFVRIFK SRRDHSNTSG NKNVMNTTNT RAKSSTLHPP GARHNKKGSK FDMNFDFDEN 240
  241 LEEEDDDDDD DEEGDDIHSQ FFQLDDDFDA KGSGASPAHK GINGMSNNKN NTYTNNRNSI 300
  301 SILDDRESSN GNIGSASRLK SHFPTSQKGK IFLTDNKNDG QKSDSLNANK GIHGDGSSAS 360
  361 GNGSVSRDGL TETESNNISD MESYINEKDL DDLNFDTVTS NINKTVSDLG GHESTNDGTA 420
  421 VMNRDSKDSR SNSNEFNAQN RDRITPGSSY GKSLLGSEYS EERYSNNDSS TMESGEMSLD 480
  481 SDMQTNTIPS HSIPMSMQKY GIYHGDDDST LNNVFDKAVL TMNSSRHPKE RRDTVISGKE 540
  541 PTSLTSSNRK FSVSSNLTST RSPLLRGHGR TSSTASSEHM KAPKVSDSVL HRARKSTLTL 600
  601 KQDHSQPSVP SSVHKSSKEG NILIEKTTDY LVSKPKASQL SNMFNKKKKR TNTNSVDVLE 660
  661 YFSFVCGDKV PNYESMGLEI YIQASKKYKR NSFTTKVRKS STIFEVIGFA LFLYSTEKKP 720
  721 DNFEEDGLTV EDISNPNNFS LKIVDEDGEP FEDNFGKLDR KSTIQSISDS EVVLCKVDDA 780
  781 EKSQNEIETP LPFETGGGLM DASTLDANSS HDTTDGTINQ LSFYKPIIGN EDDIDKTNGS 840
  841 KIIDVTVYLY PNVNPKFNYT TISVLVTSHI NDILVKYCKM KNMDPNEYAL KVLGKNYILD 900
  901 LNDTVLRLDG INKVELISKK DARELHLEKM KPDLKKPVLP TIQSNDLTPL TLEPLNSYLK 960
  961 ADAGGAVAAI PENTKVTSKA KKISTKYKLG LAKQHSSSSV ASGSVSTAGG LANGNGFFKN1020
 1021 KNSSKSSLHG TLQFHNINRS QSTMEHTPDT PNGVGDNFQD LFTGAYHKYK VWRRQQMSFI1080
 1081 NKHERTLAID GDYIYIVPPE GRIHWHDNVK TKSLHISQVV LVKKSKRVPE HFKIFVRREG1140
 1141 QDDIKRYYFE AVSGQECTEI VTRLQNLLSA YRMNHK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		AVO1 FUN12
View Details	Gavin AC, et al. (2002)		AVO1 BEM2 CEG1 CET1 CLU1 CTR9 HHF1, HHF2 LSM12 NIP1 NOP1 PDB1 PRP43 RPG1 SPT16 SSA3 SSA4 TOP2 VPS1
View Details	Ho Y, et al. (2002)		ADK1 ATP3 AVO1 BEM3 CCT2 CCT5 CLU1 CYS4 DHH1 DIG1 DIG2 DPB2 FET4 FUN12 GAL7 GPH1 GUS1 HXT6 HXT7 IDH1 ILV2 KSS1 MET16 MKK2 NOG2 NOP58 NPA3 PDC6 PDX1 PHO85 PIM1 PRO1 PUF3 RPA135 RPN12 RPN6 RRP9 RRT6 RVB1 SES1 SIS1 SLX1 SRP54 STE11 STE12 STI1 TEC1 TEM1 TFC7 TSC11 UTP20 VMA8 YBL036C YER077C YGR266W YKR051W YKU80 YLR271W YPR003C
View Details	Qiu et al. (2008)		AVO1 TSC11

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	TEM1	AVO1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..615]	deduced	N/A	No confident structure predictions are available.
2	View Details	[616..791]	MSA	3.009964	View MSA. No confident structure predictions are available.
3	View Details	[792..1015]	deduced	N/A	No confident structure predictions are available.
4	View Details	[1016..1176]	MSA	1.004953	View MSA. No confident structure predictions are available.
5	View Details	[931..1051]	deduced	N/A	No confident structure predictions are available.
6	View Details	[1052..1176]	FFAS03	0.986	Solution Structure of the Pleckstrin Homology Domain of Human Protein Kinase B beta (Pkb/Akt)

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
binding	2.0688320293797	bayes_pls_golite062009
protein binding	1.72968950672856	bayes_pls_golite062009
GTPase regulator activity	1.48723526514699	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	1.47132864518485	bayes_pls_golite062009
enzyme regulator activity	1.10772679848859	bayes_pls_golite062009
signal transducer activity	1.07208466109379	bayes_pls_golite062009
molecular transducer activity	1.07208466109379	bayes_pls_golite062009
small GTPase regulator activity	0.923576826164186	bayes_pls_golite062009
guanyl-nucleotide exchange factor activity	0.64016484101492	bayes_pls_golite062009
cytoskeletal protein binding	0.635109917427773	bayes_pls_golite062009
Ras guanyl-nucleotide exchange factor activity	0.0559591386917759	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
binding	2.52256824388179	bayes_pls_golite062009
protein binding	1.95763087285706	bayes_pls_golite062009
signal transducer activity	1.51882027675546	bayes_pls_golite062009
molecular transducer activity	1.51882027675546	bayes_pls_golite062009
GTPase regulator activity	1.50594272418871	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	1.48917924547615	bayes_pls_golite062009
actin binding	1.27269944962803	bayes_pls_golite062009
enzyme regulator activity	1.13267118951633	bayes_pls_golite062009
small GTPase regulator activity	1.10613306581585	bayes_pls_golite062009
cytoskeletal protein binding	0.972529924049714	bayes_pls_golite062009
protein domain specific binding	0.650240628628722	bayes_pls_golite062009
guanyl-nucleotide exchange factor activity	0.578612934164552	bayes_pls_golite062009
small GTPase binding	0.435164744647801	bayes_pls_golite062009
GTPase binding	0.423323808323435	bayes_pls_golite062009
receptor binding	0.391425518916624	bayes_pls_golite062009
receptor activity	0.361604826111032	bayes_pls_golite062009
transmembrane receptor activity	0.215248461701419	bayes_pls_golite062009
actin filament binding	2.21224577798385E-4	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2