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Protein Overview: TOP1

General Information:
Name(s) found: TOP1 / YOL006C [SGD]
Description(s) found:
Found 27 descriptions.
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  • Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 769 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    nucleolus [IPILink to PubMed article]
    Biological Process: mitotic chromosome condensation [IGILink to PubMed article][IMPLink to PubMed article]
    chromatin silencing at rDNA [IMPLink to PubMed article]
    DNA strand elongation involved in DNA replication [IMPLink to PubMed article]
    nuclear migration [IGILink to PubMed article][IMPLink to PubMed article]
    regulation of mitotic recombination [IMPLink to PubMed article]
    chromatin assembly or disassembly [IMPLink to PubMed article]
    DNA topological change [IDALink to PubMed article][IMPLink to PubMed article]
    regulation of transcription from RNA polymerase II promoter [IMPLink to PubMed article]
    RNA elongation from RNA polymerase II promoter [IMPLink to PubMed article]
    Molecular Function: DNA topoisomerase type I activity [IDALink to PubMed article][IMPLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTIADASKVN HELSSDDDDD VPLSQTLKKR KVASMNSASL QDEAEPYDSD EAISKISKKK  60
   61 TKKIKTEPVQ SSSLPSPPAK KSATSKPKKI KKEDGDVKVK TTKKEEQENE KKKREEEEEE 120
  121 DKKAKEEEEE YKWWEKENED DTIKWVTLKH NGVIFPPPYQ PLPSHIKLYY DGKPVDLPPQ 180
  181 AEEVAGFFAA LLESDHAKNP VFQKNFFNDF LQVLKESGGP LNGIEIKEFS RCDFTKMFDY 240
  241 FQLQKEQKKQ LTSQEKKQIR LEREKFEEDY KFCELDGRRE QVGNFKVEPP DLFRGRGAHP 300
  301 KTGKLKRRVN PEDIVLNLSK DAPVPPAPEG HKWGEIRHDN TVQWLAMWRE NIFNSFKYVR 360
  361 LAANSSLKGQ SDYKKFEKAR QLKSYIDAIR RDYTRNLKSK VMLERQKAVA IYLIDVFALR 420
  421 AGGEKSEDEA DTVGCCSLRY EHVTLKPPNT VIFDFLGKDS IRFYQEVEVD KQVFKNLTIF 480
  481 KRPPKQPGHQ LFDRLDPSIL NKYLQNYMPG LTAKVFRTYN ASKTMQDQLD LIPNKGSVAE 540
  541 KILKYNAANR TVAILCNHQR TVTKGHAQTV EKANNRIQEL EWQKIRCKRA ILQLDKDLLK 600
  601 KEPKYFEEID DLTKEDEATI HKRIIDREIE KYQRKFVREN DKRKFEKEEL LPESQLKEWL 660
  661 EKVDEKKQEF EKELKTGEVE LKSSWNSVEK IKAQVEKLEQ RIQTSSIQLK DKEENSQVSL 720
  721 GTSKINYIDP RLSVVFCKKY DVPIEKIFTK TLREKFKWAI ESVDENWRF

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) NPL6 TOP1
    View Details (MIPS) Mewes HW, et al. (2004) TOP1 TOP2
    View Details Krogan NJ, et al. (2006) AIM26 APC9 BEM3 FMP27 IRA1 MDM36 MID1 NUP42 REV1 SEC20 SML1 TCO89 TOP1 YDR396W YGL024W ZAP1 ZIM17
    View Details Gavin AC, et al. (2006) ADE5,7 REB1 TOP1
    View Details Gavin AC, et al. (2002) ABF2 ADE5,7 CKA1 CKA2 CKB1 CKB2 CTI6 DEP1 DOT6 DPB2 DPB4 EAF3 ECM16 HHF1, HHF2 HTA2 IOC3 ISW1 ISW2 ITC1 KAP114 MED4 MOT1 NAP1 NHP10 NPL6 NRD1 PHO23 POL2 PUF6 RCO1 REB1 RET1 RFA1 RFX1 RNR2 RPA135 RPC34 RPC40 RPC82 RPD3 RPO31 RSC2 RSC3 RSC58 RSC8 RVB2 RXT2 RXT3 SAP30 SDS3 SFH1 SIN3 STH1 STO1 TAF1 TFC7 TOP1 TUP1 UME1 UME6 VPS1 YBR090C-A, NHP6B
    View Details Ho Y, et al. (2002) ACO1 AIM13 CLU1 RPC82 SPT16 TOP1
    View Details Qiu et al. (2008) FOB1 TOP1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2 Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen PSF2 TOP1 1 Hazbun TR, et al. (2003)

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data TOP1 Huh WK, et al. (2003)
    View Data TOP1 and NOP56 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..123] MSA 7.064997 View MSA. No confident structure predictions are available.
    2 View Details [124..158]
    [253..290]
    [307..366]    		 PSI-BLAST 1352.0 Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment
    3 View Details [159..252]
    [291..306]    		 PSI-BLAST 1352.0 Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment
    4 View Details [367..480] PSI-BLAST 57.9794 Eukaryotic DNA topoisomerase I, catalytic core
    5 View Details [481..534] PSI-BLAST 3.221849 Tropomyosin
    6 View Details [535..615] PSI-BLAST 3.221849 Tropomyosin
    7 View Details [616..719] PSI-BLAST 3.221849 Tropomyosin
    8 View Details [720..769] ORFEUS 12.79 Eukaryotic DNA topoisomerase I, dispensable insert domain; Eukaryotic DNA topoisomerase I, catalytic core; Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • catalytic activity
    		•  0.078956976886834 bayes_pls_golite062009
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.
    7 No functions predicted.
    8 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle