Protein Overview: SRV2

General Information:
Name(s) found:	SRV2 / YNL138W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|854499\|emb\|CAA86887.1\| cyclase-associated protein [Saccharomyces cerevisiae] [NCBI NR] gi\|6324191\|ref\|NP_014261.1\| CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell [NCBI NR] gi\|172709\|gb\|AAA35094.1\| SRV2 [NCBI NR] gi\|171157\|gb\|AAA63569.1\| cyclase-associated protein [NCBI NR] gi\|134897\|sp\|P17555.1\|CAP_YEAST RecName: Full=Adenylyl cyclase-associated protein; Short=CAP [NCBI NR] gi\|1302084\|emb\|CAA96020.1\| SRV2 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|A34896 adenylate cyclase-associated protein, 70K - yeast (Saccharomyces cerevisiae) [NCBI NR] Adenylyl cyclase-associated protein OS=Saccharomyces cerevisiae GN=SRV2 PE=1 SV=1 [Swiss-Prot] SRV2 Adenylate cyclase-associated protein, 70kDa [mips] CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis [SGD] SRV2, Chr XIV from 366738-368318 [yeast_orfs2] Chr XIV from 366738-368318 [yeastorf3.fasta] (P17555) Adenylyl cyclase-associated protein (CAP) [4932.FASTACCERV.txt] SRV2 SGDID:S0005082, Chr XIV from 366741-368321, Verified ORF [yeastflysequest.fasta] Adenylyl cyclase-associated protein; N-terminal domain appears to be involved in cellular responsiveness to RAS [Saccharomyces cerevisiae]gi\|854499\|emb\|CAA86887.1\| cyclase-associated protein [Saccharomyces cerevisiae]gi\|1302084\|emb\|CAA96020.1\| SRV2 [Sac [nrdb_11152004_Chlamydomonas] SRV2 SGDID:S000005082, Chr XIV from 366743-368323, Verified ORF, "CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SRV2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SRV2 [nr-271106-contam.fasta] cyclase-associated protein [nr-271106-contam.fasta] Adenylyl cyclase-associated protein (CAP) [nr-271106-contam.fasta] cyclase-associated protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis; Srv2p [Saccha [nr-271106-contam.fasta] SRV2 SGDID:S000005082, Chr XIV from 366743-368323, Verified ORF, "CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Adenylyl cyclase-associated protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SRV2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SRV2 SGDID:S000005082, Chr XIV from 366741-368321, Genome Release 64-1-1, Verified ORF, "CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis" [yeast-030211-contam.fasta] SRV2 SGDID:S000005082, Chr XIV from 366741-368321, Genome Release 64-2-1, Verified ORF, "CAP (cyclase-associated protein); N-terminus binds adenylate cyclase and facilitates activation by RAS; N-terminus forms novel hexameric star-shaped shuriken structures that directly catalyze cofilin-mediated severing of actin filaments; C-terminus, in physically and genetically separate activity, binds and recycles cofilin bound, ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis" [20141208_orf_trans.fasta] YNL138W SGDID:S000005082, Chr XIV from 366741-368321, Genome Release 64-1-1, Verified ORF, "CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	526 amino acids
Gene Ontology:
Cellular Component:	actin cortical patch [TAS]
Biological Process:	cytoskeleton organization [TAS] pseudohyphal growth [IMP] Ras protein signal transduction [IMP]
Molecular Function:	adenylate cyclase binding [TAS] cytoskeletal protein binding [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPDSKYTMQG YNLVKLLKRL EEATARLEDV TIYQEGYIQN KLEASKNNKP SDSGADANTT  60
   61 NEPSAENAPE VEQDPKCITA FQSYIGENID PLVELSGKID TVVLDALQLL KGGFQSQLTF 120
  121 LRAAVRSRKP DYSSQTFADS LRPINENIIK LGQLKESNRQ SKYFAYLSAL SEGAPLFSWV 180
  181 AVDTPVSMVT DFKDAAQFWT NRILKEYRES DPNAVEWVKK FLASFDNLKA YIKEYHTTGV 240
  241 SWKKDGMDFA DAMAQSTKNT GATSSPSPAS ATAAPAPPPP PPAPPASVFE ISNDTPATSS 300
  301 DANKGGIGAV FAELNQGENI TKGLKKVDKS QQTHKNPELR QSSTVSSTGS KSGPPPRPKK 360
  361 PSTLKTKRPP RKELVGNKWF IENYENETES LVIDANKDES IFIGKCSQVL VQIKGKVNAI 420
  421 SLSETESCSV VLDSSISGMD VIKSNKFGIQ VNHSLPQISI DKSDGGNIYL SKESLNTEIY 480
  481 TSCSTAINVN LPIGEDDDYV EFPIPEQMKH SFADGKFKSA VFEHAG

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		COF1 CYR1 SRV2
View Details	(MIPS) Mewes HW, et al. (2004)		ABP1 AIP1 ARC40 ARP2 BEM1 BUD6 CAP1 CAP2 COF1 CRN1 DLD2 GLK1 LAS17 OYE2 PAN1 PFY1 RVS167 SAC6 SLA1 SLA2 SRV2 TPM1 TPM2 TWF1 VRP1
View Details	Krogan NJ, et al. (2006)		CYR1 SRV2
View Details	Gavin AC, et al. (2006)		CYR1 PHO81 SRV2
View Details	Gavin AC, et al. (2002)		ACT1 ADH1 CCT4 CSE1 CYR1 DHH1 DUG1 ECM29 FKS1 GCN1 GCN20 GFA1 IDH2 KAP123 KTR3 MIR1 PHO81 RGR1 SAM1 SEC7 SGN1 SLC1 SRV2 TDH3 TIF4631 TIF4632 YGR250C YHR020W
View Details	Ho Y, et al. (2002)		AIP1 ATP3 BEM2 CDC33 CDC53 COF1 COP1 CRN1 CYR1 DUF1 EDE1 FET4 FOL2 GCN1 HTB2 KAP114 LHS1 NIP1 PGM2 PHO81 POR1 PSE1 REX2 RNR2 RPG1 SAH1 SDP1 SES1 SLT2 SPO12 SRV2 STI1 TIF34 TIF35 TOS3 UBI4 UTP13 YDR306C
View Details	Qiu et al. (2008)		ABP1 ACT1 AIP1 ARC40 ARP2 BBC1 BNI1 BUD6 BZZ1 CAP1 CAP2 COF1 CRN1 LAS17 MYO3 MYO5 PAN1 PFY1 RVS161 RVS167 SAC6 SCP1 SLA1 SLA2 SPA2 SRV2 TPM1 TPM2 TWF1 VRP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	SEC10	No Comments	De Craene, J., et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SRV2	SRV2	2	Drees BL, et al. (2001)
View Screen	PFY1	SRV2	2	Drees BL, et al. (2001)
View Screen	ACT1	SRV2	2	Drees BL, et al. (2001)
View Screen	AIP1	SRV2	2	Drees BL, et al. (2001)
View Screen	SRV2	ACT1	2	Drees BL, et al. (2001)
View Screen	SRV2	TRM5	2	Drees BL, et al. (2001)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	SRV2	Huh WK, et al. (2003)
View Data	SRV2 and SAC6	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..39] [80..127] [136..247]	ORFEUS	7.03	Peroxisome proliferator activated receptor alpha, PPAR-alpha
2	View Details	[40..79] [128..135]	ORFEUS	7.03	Peroxisome proliferator activated receptor alpha, PPAR-alpha
3	View Details	[248..353]	MSA	3.030996	View MSA. No confident structure predictions are available.
4	View Details	[354..526]	PSI-BLAST	920.9897	C-terminal domain of adenylylcyclase associated protein

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
actin binding	5.81884714997094	bayes_pls_golite062009
cytoskeletal protein binding	4.39652693178112	bayes_pls_golite062009
adenylate cyclase binding	2.08284870927262	bayes_pls_golite062009
binding	1.8340549733034	bayes_pls_golite062009
enzyme binding	1.53072876683435	bayes_pls_golite062009
protein binding	0.871734119777931	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
actin binding	4.94933252861141	bayes_pls_golite062009
cytoskeletal protein binding	4.21664682046338	bayes_pls_golite062009
actin monomer binding	2.04365563273836	bayes_pls_golite062009
binding	1.57578562719958	bayes_pls_golite062009
adenylate cyclase binding	1.13353932343086	bayes_pls_golite062009
enzyme binding	0.705135358375	bayes_pls_golite062009
protein binding	0.651719649953198	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2