Protein Overview: TRM5

General Information:
Name(s) found:	TRM5 / YHR070W [SGD]
Description(s) found: Found 22 descriptions. SHOW ALL	tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya [SGD]
Organism:	Saccharomyces cerevisiae
Length:	499 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA ]
Biological Process:	ribosome biogenesis [RCA] tRNA methylation [IGI][IMP][ISS]
Molecular Function:	tRNA (guanine) methyltransferase activity [IGI][IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKIALPVFQK FNRLISSCKM SGVFPYNPPV NRQMRELDRS FFITKIPMCA VKFPEPKNIS  60
   61 VFSKNFKNCI LRVPRIPHVV KLNSSKPKDE LTSVQNKKLK TADGNNTPVT KGVLLHESIH 120
  121 SVEDAYGKLP EDALAFLKEN SAEIVPHEYV LDYDFWKAEE ILRAVLPEQF LEEVPTGFTI 180
  181 TGHIAHLNLR TEFKPFDSLI GQVILDKNNK IECVVDKVSS IATQFRTFPM KVIAGKSDSL 240
  241 VVEQKESNCT FKFDFSKVYW NSRLHTEHER LVKQYFQPGQ VVCDVFAGVG PFAVPAGKKD 300
  301 VIVLANDLNP ESYKYLKENI ALNKVAKTVK SFNMDGADFI RQSPQLLQQW IQDEEGGKIT 360
  361 IPLPLKKRHR SQQHNDQQPP QPRTKELIIP SHISHYVMNL PDSAISFLGN FRGIFAAHTK 420
  421 GATDTIQMPW VHVHCFEKYP PGDQVTEDEL HARVHARIIA ALKVTADDLP LNAVSLHLVR 480
  481 KVAPTKPMYC ASFQLPANV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ATG18 TRM5
View Details	Qiu et al. (2008)		TRM10 TRM5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SRV2	TRM5	2	Drees BL, et al. (2001)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TRM5	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..106]	deduced	N/A	No confident structure predictions are available.
2	View Details	[107..327]	PSI-BLAST	6.69897	Methyltransferase mboII
3	View Details	[328..379]	PSI-BLAST	14.221849	Hypothetical protein MJ0882
4	View Details	[380..499]	deduced	N/A	Confident ab initio structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
methyltransferase activity	4.15445027778357	bayes_pls_golite062009
N-methyltransferase activity	4.07806017452628	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	4.07153733729662	bayes_pls_golite062009
protein methyltransferase activity	3.92746815303714	bayes_pls_golite062009
S-adenosylmethionine-dependent methyltransferase activity	3.39429041855776	bayes_pls_golite062009
RNA methyltransferase activity	3.07428840534971	bayes_pls_golite062009
histone methyltransferase activity	2.99461585647365	bayes_pls_golite062009
tRNA methyltransferase activity	2.5931020126592	bayes_pls_golite062009
rRNA methyltransferase activity	2.5924681776558	bayes_pls_golite062009
DNA-methyltransferase activity	2.43145108778901	bayes_pls_golite062009
arginine N-methyltransferase activity	2.41876112831625	bayes_pls_golite062009
protein-arginine N-methyltransferase activity	2.41876112831625	bayes_pls_golite062009
transferase activity	2.22303238506686	bayes_pls_golite062009
histone-arginine N-methyltransferase activity	2.04296107639729	bayes_pls_golite062009
tRNA (cytosine-5-)-methyltransferase activity	1.88889414729709	bayes_pls_golite062009
tRNA (cytosine)-methyltransferase activity	1.88889414729709	bayes_pls_golite062009
protein-arginine omega-N monomethyltransferase activity	1.66871163498737	bayes_pls_golite062009
tRNA (adenine-N1-)-methyltransferase activity	1.29759910099835	bayes_pls_golite062009
tRNA (adenine)-methyltransferase activity	1.29759910099835	bayes_pls_golite062009
catalytic activity	1.25333584501255	bayes_pls_golite062009
histone methyltransferase activity (H4-R3 specific)	1.1103522853299	bayes_pls_golite062009
rRNA (guanine) methyltransferase activity	0.979380410238071	bayes_pls_golite062009
transcription regulator activity	0.914549843668268	bayes_pls_golite062009
protein-arginine omega-N asymmetric methyltransferase activity	0.89325812193225	bayes_pls_golite062009
DNA binding	0.86027591375666	bayes_pls_golite062009
rRNA (adenine) methyltransferase activity	0.794703281700029	bayes_pls_golite062009
rRNA (adenine-N6,N6-)-dimethyltransferase activity	0.716476158062361	bayes_pls_golite062009
site-specific DNA-methyltransferase (adenine-specific) activity	0.703178429468566	bayes_pls_golite062009
nucleic acid binding	0.616949327277483	bayes_pls_golite062009
rRNA (uridine) methyltransferase activity	0.511874381049553	bayes_pls_golite062009
RNA binding	0.417524996559564	bayes_pls_golite062009
binding	0.383237930789959	bayes_pls_golite062009
tRNA (guanine) methyltransferase activity	0.203168546434167	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2