Protein Overview: RNT1

General Information:
Name(s) found:	RNT1 / YMR239C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323895\|ref\|NP_013966.1\| RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end; Rnt1p [Saccharomyces cerevisiae] [NCBI NR] gi\|2500557\|sp\|Q02555.1\|RNT1_YEAST RecName: Full=Ribonuclease 3; AltName: Full=Ribonuclease III; Short=RNase III [NCBI NR] pir\|\|S56053 RNT1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1185416\|gb\|AAB04172.1\| Rnt1p [NCBI NR] Ribonuclease 3 OS=Saccharomyces cerevisiae GN=RNT1 PE=1 SV=1 [Swiss-Prot] RNT1 Double-stranded ribonuclease [mips] RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end; involved in polyadenylation-independent transcription termination [SGD] RNT1, Chr XIII from 748261-749676, reverse complement [yeast_orfs2] Chr XIII from 748261-749676, reverse complement [yeastorf3.fasta] Chr XIII from 749676-748261, reverse complement [yeastorf5.fasta] (Q02555) Ribonuclease III (EC 3.1.26.3) (RNase III) [4932.FASTACCERV.txt] RNT1 SGDID:S0004852, Chr XIII from 749676-748261, reverse complement, Verified ORF [yeastflysequest.fasta] RNAase III; cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end [Saccharomyces cerevisiae]gi\|2500557\|sp\|Q02555\|RNT1_YEAST Ribonuclease III (RNase III)gi\|2133107\|pir\|\|S56053 RNT1 protein - yeast (Saccharom [nrdb_11152004_Chlamydomonas] RNT1 SGDID:S000004852, Chr XIII from 749676-748261, reverse complement, Verified ORF, "RNAase III; cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RNT1 SGDID:S000004852, Chr XIII from 749676-748261, reverse complement, Verified ORF, "RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' en [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Rnt1p [nr-271106-contam.fasta] Ribonuclease III (RNase III) [nr-271106-contam.fasta] RNAase III; cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end; Rnt1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RNT1 SGDID:S000004852, Chr XIII from 749676-748261, reverse complement, Verified ORF, "RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end" [yeast-bovrabprots.fasta] [chemostatdb.fasta] RNT1 SGDID:S000004852, Chr XIII from 749676-748261, reverse complement, Verified ORF, "RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end; involved in polyadenylation-independent transcription termination" [SGDyeast.20101221.fasta] Ribonuclease 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RNT1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RNT1 SGDID:S000004852, Chr XIII from 749677-748262, Genome Release 64-1-1, reverse complement, Verified ORF, "RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end; involved in polyadenylation-independent transcription termination" [yeast-030211-contam.fasta] RNT1 SGDID:S000004852, Chr XIII from 749677-748262, Genome Release 64-2-1, reverse complement, Verified ORF, "Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes" [20141208_orf_trans.fasta] YMR239C SGDID:S000004852, Chr XIII from 749677-748262, Genome Release 64-1-1, reverse complement, Verified ORF, "RNAase III; involved in rDNA transcription and rRNA processing; also cleaves a stem-loop structure at the 3' end of U2 snRNA to ensure formation of the correct U2 3' end; involved in polyadenylation-independent transcription termination" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	471 amino acids
Gene Ontology:
Cellular Component:	nuclear outer membrane [TAS] nucleolus [IDA] nucleoplasm [IDA]
Biological Process:	ribosome biogenesis [RCA]
Molecular Function:	ribonuclease III activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGSKVAGKKK TQNDNKLDNE NGSQQRENIN TKTLLKGNLK ISNYKYLEVI QLEHAVTKLV  60
   61 ESYNKIIELS PNLVAYNEAV NNQDRVPVQI LPSLSRYQLK LAAELKTLHD LKKDAILTEI 120
  121 TDYENEFDTE QKQPILQEIS KADMEKLEKL EQVKREKREK IDVNVYENLN EKEDEEEDEG 180
  181 EDSYDPTKAG DIVKATKWPP KLPEIQDLAI RARVFIHKST IKDKVYLSGS EMINAHNERL 240
  241 EFLGDSILNS VMTLIIYNKF PDYSEGQLST LRMNLVSNEQ IKQWSIMYNF HEKLKTNFDL 300
  301 KDENSNFQNG KLKLYADVFE AYIGGLMEDD PRNNLPKIRK WLRKLAKPVI EEATRNQVAL 360
  361 EKTDKLDMNA KRQLYSLIGY ASLRLHYVTV KKPTAVDPNS IVECRVGDGT VLGTGVGRNI 420
  421 KIAGIRAAEN ALRDKKMLDF YAKQRAAIPR SESVLKDPSQ KNKKRKFSDT S

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		DRS1 GAR1 IFH1 NOP1 NPL3 NSR1 POP1 POP2 RAT1 RNT1 RRP3 SBP1 SNM1 SRD1 UTP10 UTP11 UTP13 UTP4 UTP5 UTP6 UTP7 UTP8 UTP9 YME2
View Details	Krogan NJ, et al. (2006)		BMS1 BPL1 RCL1 RNT1 SMF3
View Details	Ho Y, et al. (2002)		DIS3 EAF3 FIP1 HAS1 HPR1 KAP95 MES1 MFT1 NAM8 NOP6 NUP1 NUP2 NUP60 PAP1 PCT1 REB1 RNT1 RRP4 RRP43 RRP6 RTT103 SIF2 SIN3 SNU56 SRP1 STO1 THO2 TRA1 UME1 YPR090W
View Details	Qiu et al. (2008)		AIR1 BFR2 BRX1 CBF5 DBP3 DBP6 DBP7 DBP8 DRS1 FAL1 GAR1 HCA4 KRI1 MAK5 MTR4 NAN1 NOG2 NOP1 NOP13 NOP4 NSR1 NUG1 POP1 POP5 RAT1 RNT1 RPP1 RRP3 SNM1 SPB4 UTP10 UTP15 UTP18 UTP4 UTP5 UTP6 UTP8 UTP9

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	RNT1	Huh WK, et al. (2003)
View Data	RNT1 and NOP56	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..112]	MSA	1.00596	View MSA. No confident structure predictions are available.
2	View Details	[113..180]	deduced	N/A	Confident ab initio structure predictions are available.
3	View Details	[181..359]	PSI-BLAST	25.69897	RNase III endonuclease catalytic domain
4	View Details	[360..471]	PSI-BLAST	10.154902	Staufen, domain III

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: RNT1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: