YRC Logo
PROTEIN SEARCH:
Descriptions Names[Advanced Search]

Protein Overview: MPH1

General Information:
Name(s) found: MPH1 / YIR002C [SGD]
Description(s) found:
SHOW ONLY BEST
  • gi|731905|sp|P40562.1|MPH1_YEAST RecName: Full=ATP-dependent DNA helicase MPH1; AltName: Full=Mutator phenotype protein 1 [NCBI NR]
  • gi|6322192|ref|NP_012267.1| Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, mutations confer a mutator phenotype; Mph1p [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S48436 probable RNA helicase YIR002c - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|557851|emb|CAA86204.1| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR]
  • Putative ATP-dependent RNA helicase YIR002C - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot]
  • ATP-dependent DNA helicase MPH1 OS=Saccharomyces cerevisiae GN=MPH1 PE=1 SV=1 [Swiss-Prot]
  • MPH1 Protection of the genome from spontaneous and chemically induced damage [mips]
  • Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, binds to flap DNA and stimulates activity of Rad27p and Dna2p; mutations confer a mutator phenotype [SGD]
  • MPH1, Chr IX from 357412-360393, reverse complement [yeast_orfs2]
  • Chr IX from 357412-360393, reverse complement [yeastorf3.fasta]
  • Chr IX from 360393-357412, reverse complement [yeastorf5.fasta]
  • (P40562) Putative ATP-dependent RNA helicase YIR002C [4932.FASTACCERV.txt]
  • MPH1 SGDID:S0001441, Chr IX from 360393-357412, reverse complement, Verified ORF [yeastflysequest.fasta]
  • Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, mutations confer a mutator phenotype [Saccharomyces cerevisiae]gi|557851|emb|CAA86204.1| unnamed protein product [Sacchar [nrdb_11152004_Chlamydomonas]
  • (P40562) Putative ATP-dependent RNA helicase YIR002C (EC 3.6.1.-) [cerv4932.FASTAC]
  • MPH1 SGDID:S000001441, Chr IX from 360393-357412, reverse complement, Verified ORF, "Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, mutations confer a mutator phenotype" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • MPH1 SGDID:S000001441, Chr IX from 360393-357412, reverse complement, Verified ORF, "Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, binds to flap DNA and stimulates acti [yeast-200209-contam.fasta]
  • Putative ATP-dependent RNA helicase YIR002C [nr-271106-contam.fasta]
  • unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, mutations confer a mutator phenotype; Mph1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • MPH1 SGDID:S000001441, Chr IX from 360393-357412, reverse complement, Verified ORF, "Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, binds to flap DNA and stimulates activity of Rad27p and Dna2p; mutations confer a mutator phenotype" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • ATP-dependent DNA helicase MPH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MPH1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • MPH1 SGDID:S000001441, Chr IX from 360396-357415, Genome Release 64-1-1, reverse complement, Verified ORF, "Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, binds to flap DNA and stimulates activity of Rad27p and Dna2p; mutations confer a mutator phenotype" [yeast-030211-contam.fasta]
  • MPH1 SGDID:S000001441, Chr IX from 360396-357415, Genome Release 64-2-1, reverse complement, Verified ORF, "3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA in error-free bypass pathway, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; similar to FANCM human Fanconi anemia complementation group protein that with MHF complex is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases" [20141208_orf_trans.fasta]
  • YIR002C SGDID:S000001441, Chr IX from 360396-357415, Genome Release 64-1-1, reverse complement, Verified ORF, "Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, binds to flap DNA and stimulates activity of Rad27p and Dna2p; mutations confer a mutator phenotype" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 993 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    Biological Process: DNA repair [IMPLink to PubMed article]
    Molecular Function: RNA helicase activity [IMPLink to PubMed article][ISSLink to PubMed article]
    3'-5' DNA helicase activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASADDYFSD FEDDELDKLY EKAINKSVKE TITRRAVPVQ KDLHDNVLPG QKTVYEEIQR  60
   61 DVSFGPTHHE LDYDALSFYV YPTNYEVRDY QYTIVHKSLF QNTLCAIPTG MGKTFIASTV 120
  121 MLNYFRWTKK AKIIFTAPTR PLVAQQIKAC LGITGIPSDQ TAILLDKSRK NREEIWANKR 180
  181 VFFATPQVVE NDLKRGVLDP KDIVCLVIDE AHRATGSSAY TNVVKFIDRF NSSYRLLALT 240
  241 ATPASDLEGV QEVVNNLDIS KIEIRTEESM DIVKYMKKRK KEKIEVPLLL EIEDIIEQLG 300
  301 MAVKPVLQQA IELGIYEECD PSQINAFKAM QQSQKIIANP TIPEGIKWRN FFILQLLNNV 360
  361 GQMLKRLKIY GIRTFFNYFQ NKCTEFTTKY NLKKSTNKIA AEFYYHPILK NIKNQCENYL 420
  421 SDPKFVGHGK LQCVRDELMD FFQKRGSDSR VIIFTELRES ALEIVKFIDS VADDQIRPHI 480
  481 FIGQARAKEG FDEVKYTRKH APKGRKKVER LHRQEQEKFL EAERTKRAAN DKLERSARRT 540
  541 GSSEEAQISG MNQKMQKEVI HNFKKGEYNV LVCTSIGEEG LDIGEVDLII CYDTTSSPIK 600
  601 NIQRMGRTGR KRDGKIVLLF SSNESYKFER AMEDYSTLQA LISKQCIDYK KSDRIIPEDI 660
  661 IPECHETLIT INDENEIINE MEDVDEVIRY ATQCMMGKKV KPKKAITKKK RVQENKKPKK 720
  721 FFMPDNVETS IVSASTLINK FLVNESGGKQ LVTSNENPSK KRKIFKALDN LENDSTEEAS 780
  781 SSLETEDEEV SDDNNVFIAE GQNGCQKDLE TAIIRTGESL TTLKPLHNFE RPNMALFVND 840
  841 CGLPTKIEKN VKDIRGNQHN LEKEKSCTVD KNNMVLSLDD WNFFRNRYIP EGVSFDVEPN 900
  901 FVQYTKGVKV PHCHKVSKII TLFNDESNDN KKRTIDMNYT KCLARGMLRD EKKFVKVNDK 960
  961 SQVDNNSVNH DSSQSFTLSN AELDDILGSD SDF

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) MGM101 MPH1 MSH2 MSH6 RAD52 RFA1 RFA2
    View Details Gavin AC, et al. (2006) MPH1 MSH6 RAD52 RFA1
    View Details Gavin AC, et al. (2002) CMR1 DNA2 ECM29 HDA1 HDA2 HDA3 HSC82 KAP95 LCD1 MEC1 MGM101 MLH1 MPH1 MRPS28 MSH2 MSH3 MSH6 OLA1 PGK1 PMS1 PRO2 RAD16 RAD23 RAD34 RAD4 RAD52 RFA1 RFA2 RFA3 RIM1 RNH1 RPN10 RPN12 RPN3 RPN6 RPN8 RPN9 RPT2 RPT3 RPT6 RSC8 RVB2 SGS1 SHS1 SRO7 SSL1 TFA1 TFB1 TFB4 TIF1, TIF2 TOP3 VAS1 VPS1 YHR122W
    View Details Ho Y, et al. (2002) AI1 CDC28 CEG1 CKA1 CKA2 CKB1 CKB2 FKH1 FUN12 FYV8 GCD2 GCD7 GCN3 HHF1, HHF2 HHO1 HTB1 IMD2 IMD3 IMD4 MAG1 MBP1 MGM101 MPH1 MSH2 NET1 NOP1 NOP12 RET1 RPC82 RRP1 SEC2 SIN3 SUI2 SUI3 TIF4631 TIF4632 UBP12 URE2 UTP22 YGR017W YMR144W

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #14 Mitotic Prep2-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data MPH1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..286] PSI-BLAST 68.69897 Initiation factor 4a
    2 View Details [287..486] PSI-BLAST 145.228787 Putative DEAD box RNA helicase
    3 View Details [487..634] PSI-BLAST 145.228787 Putative DEAD box RNA helicase
    4 View Details [635..737] PSI-BLAST 65.522879 Nucleotide excision repair enzyme UvrB
    5 View Details [738..993] deduced N/A No confident structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle