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Protein Overview: ROK1

General Information:
Name(s) found: ROK1 / YGL171W [SGD]
Description(s) found:
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  • gi|971382|emb|CAA59758.1| putative ATP dependent RNA helicase [Saccharomyces cerevisiae] [NCBI NR]
  • gi|6321267|ref|NP_011344.1| ATP-dependent RNA helicase of the DEAD box family; required for 18S rRNA synthesis; Rok1p [Saccharomyces cerevisiae] [NCBI NR]
  • gi|607182|emb|CAA84384.1| ATP-dependent RNA helicase [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S59649 ATP-dependent RNA helicase ROK1 - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|1322774|emb|CAA96883.1| ROK1 [Saccharomyces cerevisiae] [NCBI NR]
  • gi|1173121|sp|P45818.1|ROK1_YEAST RecName: Full=ATP-dependent RNA helicase ROK1; AltName: Full=Rescuer of KEM1 protein 1 [NCBI NR]
  • ATP-dependent RNA helicase ROK1 OS=Saccharomyces cerevisiae GN=ROK1 PE=1 SV=1 [Swiss-Prot]
  • ROK1 ATP-dependent RNA helicase [mips]
  • ATP-dependent RNA helicase of the DEAD box family; required for 18S rRNA synthesis [SGD]
  • ROK1, Chr VII from 182394-184088 [yeast_orfs2]
  • Chr VII from 182394-184088 [yeastorf3.fasta]
  • (P45818) ATP-dependent RNA helicase ROK1 (EC 3.6.1.-) [4932.FASTACCERV.txt]
  • ROK1 SGDID:S0003139, Chr VII from 182394-184088, Verified ORF [yeastflysequest.fasta]
  • ROK1 SGDID:S0003139, Chr VII from 182396-184090, Verified ORF [10112005cervSGD.fasta]
  • ATP-dependent RNA helicase of the DEAD box family; required for 18S rRNA synthesis [Saccharomyces cerevisiae]gi|1322774|emb|CAA96883.1| ROK1 [Saccharomyces cerevisiae]gi|971382|emb|CAA59758.1| putative ATP dependent RNA helicase [Saccharomyces cerevisia [nrdb_11152004_Chlamydomonas]
  • ROK1 SGDID:S000003139, Chr VII from 182396-184090, Verified ORF, "ATP-dependent RNA helicase of the DEAD box family; required for 18S rRNA synthesis" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • ATP-dependent RNA helicase ROK1 (Rescuer of KEM1 protein 1) [nr-271106-contam.fasta]
  • ATP-dependent RNA helicase of the DEAD box family; required for 18S rRNA synthesis; Rok1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • putative ATP dependent RNA helicase [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • ATP-dependent RNA helicase [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • ROK1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • [chemostatdb.fasta]
  • ATP-dependent RNA helicase ROK1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ROK1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • ROK1 SGDID:S000003139, Chr VII from 182390-184084, Genome Release 64-1-1, Verified ORF, "ATP-dependent RNA helicase of the DEAD box family; required for 18S rRNA synthesis" [yeast-030211-contam.fasta]
  • ROK1 SGDID:S000003139, Chr VII from 182390-184084, Genome Release 64-2-1, Verified ORF, "RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation" [20141208_orf_trans.fasta]
  • YGL171W SGDID:S000003139, Chr VII from 182390-184084, Genome Release 64-1-1, Verified ORF, "ATP-dependent RNA helicase of the DEAD box family; required for 18S rRNA synthesis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 564 amino acids

    Gene Ontology:
    Cellular Component: nuclear outer membrane [TASLink to PubMed article]
    nucleolus [TASLink to PubMed article]
    Biological Process: snoRNA localization [IMP]
    ribosome biogenesis [IEA]
    rRNA processing [IEA][TAS]
    Molecular Function: ATPase activity [IDALink to PubMed article]
    ATP-dependent RNA helicase activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDIFRVLTRG ASVKKESGPK AKAADYSVIN GNDENHKEDN NESQIVKELD FFRNKRIISK  60
   61 VEDDREKTTE NDSPNKEEKS GNDDGLIKPV ITNTVEASAL RKSYKGNVSG IDIPLPIGSF 120
  121 EDLISRFSFD KRLLNNLIEN GFTEPTPIQC ECIPVALNNR DVLACGPTGS GKTLAFLIPL 180
  181 VQQIIDDKQT AGLKGLIISP TKELANQIFI ECFKLSYKIF LEKKRPLQVA LLSKSLGAKL 240
  241 KNKVVSDKKY DIIISTPLRL IDVVKNEALD LSKVKHLIFD EADKLFDKTF VEQSDDILSA 300
  301 CREPSLRKAM FSATIPSNVE EIAQSIMMDP VRVIIGHKEA ANTNIEQKLI FCGNEEGKLI 360
  361 AIRQLVQEGE FKPPIIIFLE SITRAKALYH ELMYDRINVD VIHAERTALQ RDRIIERFKT 420
  421 GELWCLICTD VLARGIDFKG VNLVINYDVP GSSQAYVHRI GRTGRGGRSG KAITFYTKQD 480
  481 SVAIKPIINV MKQSGCEVSE WMDKMAKMTR KEKESIKNGK AHKERKQITT VPKMDKAKRR 540
  541 RQQEMIAASK RRKNEELSKK HFSK

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) BFR2 DIP2 EMG1 ENP2 IMP3 KRE33 MPP10 NAN1 NOC4 PWP2 ROK1 UTP10 UTP20 UTP21 UTP30 UTP6 UTP7
    View Details Krogan NJ, et al. (2006) ARI1 POL4 ROK1
    View Details Gavin AC, et al. (2006) CMS1 DIP2 ENP1 ENP2 KRE33 KRR1 MRD1 NAN1 NOC4 NOP14 NOP58 PNO1 PWP2 ROK1 RPS1B RRP9 UTP10 UTP13 UTP15 UTP18 UTP21 UTP22 UTP4 UTP6 UTP7 UTP8 UTP9
    View Details Gavin AC, et al. (2002) ADE4 ASC1 BFR2 BMS1 BRX1 BUD21 CBF5 CFT2 CKA1 CKA2 CKB1 CKB2 CMS1 DBP8 DIA4 DIM1 DIP2 DIS3 ECM16 ECM29 EMG1 ENP1 ENP2 ERO1 ESF1 ESF2 FAP7 FIP1 GCN1 GLC7 HAS1 HCA4 HHF1, HHF2 HSL1 HTA2 IMP3 IMP4 KRE33 KRR1 LCP5 LTV1 MPP10 MRD1 MVD1 NAN1 NOB1 NOC2 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PFS2 PNO1 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 RCL1 REF2 RML2 RNA14 ROK1 RPP2B RRP12 RRP7 RRP9 RTG2 SAM1 SCL1 SEC21 SLX9 SOF1 SRO9 TIF1, TIF2 TSR1 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 YOR059C YSH1
    View Details Ho Y, et al. (2002) CIC1 DUG2 ERB1 ROK1 UTP10 UTP15
    View Details Qiu et al. (2008) DBP10 DBP3 DBP6 DBP7 DBP9 DHR2 DRS1 HAS1 HCA4 MAK5 NOG2 NUG1 ROK1 RRP3 SPB4

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2 Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen BRR2 ROK1 1 Unpublished Fields Lab Data
    View Screen HRT1 ROK1 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

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    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..95] deduced N/A No confident structure predictions are available.
    2 View Details [96..340] PSI-BLAST 539.228787 Putative DEAD box RNA helicase
    3 View Details [341..497] PSI-BLAST 539.228787 Putative DEAD box RNA helicase
    4 View Details [498..564] deduced N/A Confident ab initio structure predictions are available.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle