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Protein Overview: PRP5

General Information:
Name(s) found: PRP5 / YBR237W [SGD]
Description(s) found:
Found 28 descriptions.
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  • RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 849 amino acids

    Gene Ontology:
    Cellular Component: spliceosomal complex [TASLink to PubMed article]
    Biological Process: RNA splicing [IEA]
    nuclear mRNA branch site recognition [TAS]
    mRNA processing [IEA]
    Molecular Function: ATP-dependent RNA helicase activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 METIDSKQNI NRESLLEERR KKLAKWKQKK AQFDAQKEHQ TSRNDIVTNS LEGKQTTEKF  60
   61 TERQERVKEE LRKRKNEFRK SDEPVSVKPS KKKSKRSKVK KKISFDFSDD DDSEIGVSFR 120
  121 SKEHIQKAPE HDNEKDPLDE FMTSLKEEKM SNSKGMYDRG DILDVEDQLF ELGGTDDEDV 180
  181 EDNTDNSNIA KIAKLKAKKR VKQIYYSPEE LEPFQKNFYI ESETVSSMSE MEVEELRLSL 240
  241 DNIKIKGTGC PKPVTKWSQL GLSTDTMVLI TEKLHFGSLT PIQSQALPAI MSGRDVIGIS 300
  301 KTGSGKTISY LLPLLRQVKA QRPLSKHETG PMGLILAPTR ELALQIHEEV TKFTEADTSI 360
  361 RSVCCTGGSE MKKQITDLKR GTEIVVATPG RFIDILTLND GKLLSTKRIT FVVMDEADRL 420
  421 FDLGFEPQIT QIMKTVRPDK QCVLFSATFP NKLRSFAVRV LHSPISITIN SKGMVNENVK 480
  481 QKFRICHSED EKFDNLVQLI HERSEFFDEV QSENDGQSSD VEEVDAKAII FVSSQNICDF 540
  541 ISKKLLNAGI VTCAIHAGKP YQERLMNLEK FKREKNSILL CTEVLSRGLN VPEVSLVIIY 600
  601 NAVKTFAQYV HTTGRTARGS RSGTAITLLL HDELSGAYIL SKAMRDEEIK ALDPLQAKEL 660
  661 QEMSAKFESG MKKGKFRLSK GFGGKGLENI KSKREEAQNK DLELKKNDKR SDDLEKKISN 720
  721 PREGHDSVSE SSALIPRLNY ELFKESTDGS IIFYAKVYIN DLPQIVRWEA TKNTTLLFIK 780
  781 HETGCSITNK GKFYPEGKEP KNENDEPKLY LLIEGQDEKD IQLSIELLEQ KVKEGVVKAA 840
  841 SLSLKSTKY

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details (MIPS) Mewes HW, et al. (2004) AAR2 BRR1 BRR2 CBC2 CDC40 CWC15 CWC2 CWC22 CWC23 DHR2 LSM4 MAK5 MUD2 PRP16 PRP18 PRP2 PRP22 PRP24 PRP28 PRP31 PRP38 PRP39 PRP4 PRP40 PRP43 PRP5 PRP6 PRP8 SLU7 SMD1 SMD3 SME1 SNP1 SPP2 SPP381 STO1 YHC1
    View Details Krogan NJ, et al. (2006) ADE2 BSC2 CWH41 DBF4 DIG1 ECM29 HAL5 LAA1 MRC1 OPI9 PEX32 PRP5 PTH2 RGA2 RMA1 RPN3 SIR1 STE12 THR1 TLG2 VPS45 YDL199C YEL057C YPL035C
    View Details Qiu et al. (2008) AAR2 BRR2 BUD31 CBC2 CDC40 CEF1 CLF1 CUS1 CUS2 CWC15 CWC2 CWC21 CWC22 CWC23 CWC25 DIB1 ECM2 HSH155 IST3 ISY1 LEA1 LSM2 LSM3 LSM4 LSM5 LSM7 LSM8 LUC7 MAK5 MSL1 MUD1 MUD2 NAM8 NTC20 PRP11 PRP16 PRP18 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP46 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP2 SPP381 SPP382 STO1 SUB2 SYF1 SYF2 YHC1 YJU2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen CDC34 PRP5 1 Unpublished Fields Lab Data
    View Screen ATG17 PRP5 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data PRP5 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..218] MSA 8.274997 View MSA. No confident structure predictions are available.
    2 View Details [219..278] PSI-BLAST 461.0 Initiation factor 4a
    3 View Details [279..473]
    [671..746]    		 PSI-BLAST 13.30103 RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
    4 View Details [474..670] PSI-BLAST 13.30103 RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
    5 View Details [747..849] ORFEUS 8.62 RNA splicing factor 1

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5
    Term Confidence Notes
  • RNA binding
    		•  6.35205331138538 bayes_pls_golite062009
    		• 4.77327442333813 bayes_pls_golite062009
  • mRNA binding
    		•  4.67566555881601 bayes_pls_golite062009
  • nucleic acid binding
    		•  4.53130750638836 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  3.73042056823822 bayes_pls_golite062009
  • translation regulator activity
    		•  3.73023228922019 bayes_pls_golite062009
  • binding
    		•  3.31439627344982 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.27014249542806 bayes_pls_golite062009
  • DNA binding
    		•  2.0152088603757 bayes_pls_golite062009
  • poly-pyrimidine tract binding
    		•  1.75736205236351 bayes_pls_golite062009
  • transcription repressor activity
    		•  1.72867277839034 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.40423326576059 bayes_pls_golite062009
  • transcription factor activity
    		•  1.30878011063724 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  1.27324890490526 bayes_pls_golite062009
  • protein binding
    		•  1.09698608157472 bayes_pls_golite062009
  • ribonuclease activity
    		•  0.925909354724523 bayes_pls_golite062009
  • single-stranded RNA binding
    		•  0.795008427109843 bayes_pls_golite062009
  • hydrolase activity
    		•  0.715182212485288 bayes_pls_golite062009
  • mRNA 3'-UTR binding
    		•  0.370287528838428 bayes_pls_golite062009
  • transcription activator activity
    		•  0.281896115904289 bayes_pls_golite062009
  • transcription factor binding
    		•  0.207514957974522 bayes_pls_golite062009
  • exoribonuclease activity
    		•  0.150228013820667 bayes_pls_golite062009
  • catalytic activity
    		•  0.1435046019283 bayes_pls_golite062009
  • exoribonuclease activity, producing 5'-phosphomonoesters
    		•  0.110425673333101 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  0.0108102459345405 bayes_pls_golite062009


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle