Protein Overview: YSH1

General Information:
Name(s) found:	YSH1 / YLR277C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74644951\|sp\|Q06224.1\|YSH1_YEAST RecName: Full=Endoribonuclease YSH1; AltName: Full=mRNA 3'-end-processing protein YSH1; AltName: Full=Yeast 73 kDa homolog 1 [NCBI NR] gi\|6323307\|ref\|NP_013379.1\| Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex required for 3' processing of mRNAs; Ysh1p [Saccharomyces cerevisiae] [NCBI NR] gi\|577190\|gb\|AAB67367.1\| Ysh1p: subunit of polyadenylation factor I (PF I) [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S51413 probable membrane protein YLR277c - yeast (Saccharomyces cerevisiae) [NCBI NR] Endoribonuclease YSH1 OS=Saccharomyces cerevisiae GN=YSH1 PE=1 SV=1 [Swiss-Prot] YSH1 Component of pre-mRNA polyadenylation factor PF I [mips] Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs [SGD] YSH1, Chr XII from 697156-699495, reverse complement [yeast_orfs2] Chr XII from 697156-699495, reverse complement [yeastorf3.fasta] Chr XII from 699495-697156, reverse complement [yeastorf5.fasta] >Q06224 (Q06224) Chromosome XII COSMID 9328 [4932.FASTACCERV.txt] YSH1 SGDID:S0004267, Chr XII from 699497-697158, reverse complement, Verified ORF [yeastflysequest.fasta] Ysh1p [Saccharomyces cerevisiae]gi\|1077401\|pir\|\|S51413 probable membrane protein YLR277c - yeast (Saccharomyces cerevisiae)gi\|577190\|gb\|AAB67367.1\| Ysh1p: subunit of polyadenylation factor I (PF I) [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] (Q06224) Ysh1p: subunit of polyadenylation factor I (PF I) [cerv4932.FASTAC] YSH1 SGDID:S000004267, Chr XII from 699497-697158, reverse complement, Verified ORF, "Putative endonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex required for 3' processing of mRNAs" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] YSH1 SGDID:S000004267, Chr XII from 699497-697158, reverse complement, Verified ORF, "Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex required for 3' processing of mRNAs" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] YSH1 SGDID:S000004267, Chr XII from 699497-697158, reverse complement, Verified ORF, "Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of [SGD_S-cerevisiae_na_05-08-2009_con_reversed.fasta] Putative endonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex required for 3' processing of mRNAs; Ysh1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Endoribonuclease YSH1 (mRNA 3'-end-processing protein YSH1) (Yeast 73 kDa homolog 1) [nr-271106-contam.fasta] Ysh1p: subunit of polyadenylation factor I (PF I) [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YSH1 SGDID:S000004267, Chr XII from 699497-697158, reverse complement, Verified ORF, "Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs" [scerevisihumanrev.fasta] [chemostatdb.fasta] Endoribonuclease YSH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YSH1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] YSH1 SGDID:S000004267, Chr XII from 699495-697156, Genome Release 64-1-1, reverse complement, Verified ORF, "Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs" [yeast-030211-contam.fasta] YSH1 SGDID:S000004267, Chr XII from 699495-697156, Genome Release 64-2-1, reverse complement, Verified ORF, "Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YLR277C SGDID:S000004267, Chr XII from 699495-697156, Genome Release 64-1-1, reverse complement, Verified ORF, "Putative endoribonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	779 amino acids
Gene Ontology:
Cellular Component:	mRNA cleavage and polyadenylation specificity factor complex [IPI ] mRNA cleavage factor complex [IPI]
Biological Process:	mRNA polyadenylation [IDA][TAS] mRNA cleavage [IDA][TAS]
Molecular Function:	RNA binding [IC] endoribonuclease activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MERTNTTTFK FFSLGGSNEV GRSCHILQYK GKTVMLDAGI HPAYQGLASL PFYDEFDLSK  60
   61 VDILLISHFH LDHAASLPYV MQRTNFQGRV FMTHPTKAIY RWLLRDFVRV TSIGSSSSSM 120
  121 GTKDEGLFSD EDLVDSFDKI ETVDYHSTVD VNGIKFTAFH AGHVLGAAMF QIEIAGLRVL 180
  181 FTGDYSREVD RHLNSAEVPP LSSNVLIVES TFGTATHEPR LNRERKLTQL IHSTVMRGGR 240
  241 VLLPVFALGR AQEIMLILDE YWSQHADELG GGQVPIFYAS NLAKKCMSVF QTYVNMMNDD 300
  301 IRKKFRDSQT NPFIFKNISY LRNLEDFQDF GPSVMLASPG MLQSGLSRDL LERWCPEDKN 360
  361 LVLITGYSIE GTMAKFIMLE PDTIPSINNP EITIPRRCQV EEISFAAHVD FQENLEFIEK 420
  421 ISAPNIILVH GEANPMGRLK SALLSNFASL KGTDNEVHVF NPRNCVEVDL EFQGVKVAKA 480
  481 VGNIVNEIYK EENVEIKEEI AAKIEPIKEE NEDNLDSQAE KGLVDEEEHK DIVVSGILVS 540
  541 DDKNFELDFL SLSDLREHHP DLSTTILRER QSVRVNCKKE LIYWHILQMF GEAEVLQDDD 600
  601 RVTNQEPKVK EESKDNLTNT GKLILQIMGD IKLTIVNTLA VVEWTQDLMN DTVADSIIAI 660
  661 LMNVDSAPAS VKLSSHSCDD HDHNNVQSNA QGKIDEVERV KQISRLFKEQ FGDCFTLFLN 720
  721 KDEYASNKEE TITGVVTIGK STAKIDFNNM KILECNSNPL KGRVESLLNI GGNLVTPLC

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CFT1 CFT2 FIP1 GLC7 MPE1 PAP1 PCF11 PFS2 PTA1 PTI1 REF2 RNA14 SSU72 SWD2 YSH1 YTH1
View Details	(MIPS) Mewes HW, et al. (2004)		CFT1 CFT2 FIP1 PTA1 YSH1
View Details	Krogan NJ, et al. (2006)		AIM1 CFT1 CFT2 ECM21 FIP1 GIP4 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 SSU72 SWD2 SYC1 YBL010C YLR358C YSH1 YTH1
View Details	Gavin AC, et al. (2006)		CFT1 CFT2 FIP1 MPE1 PAP1 PFS2 PTA1 YSH1 YTH1
View Details	Gavin AC, et al. (2002)		ACT1 ADE4 AIM31 BFR2 BMS1 BUD21 CBF5 CFT1 CFT2 CKA1 CLP1 DBP8 DIP2 ECM16 ECM29 EMG1 ENO2 ENP1 ENP2 ERO1 ESF1 ESF2 FIP1 GCN1 GLC7 HAS1 HCA4 HHF1, HHF2 HSL1 HTA1 HTA2 IMP3 IMP4 KRE33 KRR1 MPE1 MPP10 MVD1 NAN1 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PCF11 PFK1 PFS2 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 REF2 RML2 RNA14 RNA15 ROK1 RPP2B RRP9 RSA3 RTG2 SCL1 SEC13 SEC21 SEC31 SOF1 SRO9 SSA3 SSU72 SYC1 TAF6 TIF1, TIF2 TIF4632 TYE7 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 VID24 VMR1 VPS53 YCL046W YSH1 YTH1
View Details	Qiu et al. (2008)		CFT1 CFT2 CLP1 HRP1 PAP1 PFS2 PTA1 REF2 RNA14 RNA15 SSU72 SWD2 YSH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	YSH1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..170]	PSI-BLAST	14.522879	Zn metallo-beta-lactamase
2	View Details	[171..431]	MSA	4.502993	View MSA. No confident structure predictions are available.
3	View Details	[432..529]	MSA	1.114992	View MSA. No confident structure predictions are available.
4	View Details	[530..779]	MSA	2.052967	View MSA. No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Protein Overview: YSH1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: