Protein Overview: TPK3

General Information:
Name(s) found:	TPK3 / YKL166C [SGD]
Description(s) found: Found 35 descriptions. SHOW ALL	cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase [SGD]
Organism:	Saccharomyces cerevisiae
Length:	398 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [TAS] cAMP-dependent protein kinase complex [IMP][ISS][TAS]
Biological Process:	positive regulation of apoptosis [IGI] pseudohyphal growth [IMP] protein amino acid phosphorylation [TAS]
Molecular Function:	cAMP-dependent protein kinase activity [TAS] protein serine/threonine kinase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MYVDPMNNNE IRKLSITAKT ETTPDNVGQD IPVNAHSVHE ECSSNTPVEI NGRNSGKLKE  60
   61 EASAGICLVK KPMLQYRDTS GKYSLSDFQI LRTLGTGSFG RVHLIRSNHN GRFYALKTLK 120
  121 KHTIVKLKQV EHTNDERRML SIVSHPFIIR MWGTFQDSQQ VFMVMDYIEG GELFSLLRKS 180
  181 QRFPNPVAKF YAAEVCLALE YLHSKDIIYR DLKPENILLD KNGHIKITDF GFAKYVPDVT 240
  241 YTLCGTPDYI APEVVSTKPY NKSVDWWSFG VLIYEMLAGY TPFYNSNTMK TYENILNAEL 300
  301 KFPPFFHPDA QDLLKKLITR DLSERLGNLQ NGSEDVKNHP WFNEVIWEKL LARYIETPYE 360
  361 PPIQQGQGDT SQFDRYPEEE FNYGIQGEDP YMDLMKEF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BCY1 TPK1 TPK2 TPK3
View Details	(MIPS) Mewes HW, et al. (2004)		BCY1 TPK1 TPK2 TPK3
View Details	Gavin AC, et al. (2002)		BCY1 TPK2 TPK3
View Details	Ho Y, et al. (2002)		ADE5,7 BCY1 CAR2 CDC33 CPR6 CYS4 FET4 GND1 GPH1 GTO1 JJJ1 LAA1 LSP1 LYS1 MRS6 PFK1 PIL1 PKH1 POR1 RIM15 SAH1 SEC27 SNO2 SSN8 TCP1 THI22 THO2 TIF1, TIF2 TPK1 TPK2 TPK3 VPS13 VPS21 YBR028C YCK1 YCK2 YCK3 YGR111W YHR033W YHR214W-A YPT52 YPT53 YPT7
View Details	Qiu et al. (2008)		TPK1 TPK2 TPK3

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	TPK3	TPK3	2	Uetz P, et al. (2000)
View Screen	CDC48	TPK3	1	Unpublished Fields Lab Data
View Screen	RAD6	TPK3	1	Unpublished Fields Lab Data
View Screen	SNF4	TPK3	1	Unpublished Fields Lab Data
View Screen	NTA1	TPK3	1	Unpublished Fields Lab Data
View Screen	TPK3	KIN3	1	Unpublished Fields Lab Data
View Screen	TPK3	TAF10	1	Unpublished Fields Lab Data
View Screen	TPK3	GEP4	1	Unpublished Fields Lab Data
View Screen	TPK3	UBA4	1	Unpublished Fields Lab Data
View Screen	TPK3	AHC1	1	Unpublished Fields Lab Data
View Screen	TPK3	SSN3	1	Unpublished Fields Lab Data
View Screen	TPK3	RDS2	1	Unpublished Fields Lab Data
View Screen	PRP16	TPK3	1	Unpublished Fields Lab Data
View Screen	FAR10	TPK3	1	Unpublished Fields Lab Data
View Screen	NOP13	TPK3	1	Unpublished Fields Lab Data
View Screen	TEL1	TPK3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	TPK3	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..78]	deduced	N/A	No confident structure predictions are available.
2	View Details	[79..137] [151..167] [363..398]	PSI-BLAST	1860.0	cAMP-dependent PK, catalytic subunit
3	View Details	[138..150] [168..362]	PSI-BLAST	1860.0	cAMP-dependent PK, catalytic subunit

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
cyclic nucleotide-dependent protein kinase activity	4.97855712164511	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
cAMP-dependent protein kinase activity	4.66277812893755	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
protein tyrosine kinase activity	2.8153155526437	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
transmembrane receptor activity	2.59526003830791	bayes_pls_golite062009
transmembrane receptor protein kinase activity	2.54265774272269	bayes_pls_golite062009
signal transducer activity	2.40586892801446	bayes_pls_golite062009
molecular transducer activity	2.40586892801446	bayes_pls_golite062009
receptor activity	2.39311927684508	bayes_pls_golite062009
telethonin binding	2.27062712605521	bayes_pls_golite062009
tubulin binding	2.18641675163967	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase activity	2.16831387024372	bayes_pls_golite062009
cytoskeletal protein binding	2.12615078788254	bayes_pls_golite062009
receptor signaling protein activity	2.10379226653669	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
microtubule binding	1.88110801192607	bayes_pls_golite062009
actin binding	1.87817875565699	bayes_pls_golite062009
acetylcholine receptor activator activity	1.83545227594542	bayes_pls_golite062009
phosphoinositide 3-kinase binding	1.82515074380651	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.81249386965144	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
insulin receptor substrate binding	1.62657794525303	bayes_pls_golite062009
transcription regulator activity	1.56748951434625	bayes_pls_golite062009
JUN kinase kinase kinase activity	1.41172362253967	bayes_pls_golite062009
alpha-actinin binding	1.38861866085902	bayes_pls_golite062009
DNA binding	1.35333135345112	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.32254739984688	bayes_pls_golite062009
gamma-tubulin binding	1.28511591054866	bayes_pls_golite062009
kinase binding	1.26086833316556	bayes_pls_golite062009
ErbB-3 class receptor binding	1.25765701903393	bayes_pls_golite062009
protein binding	1.22565748038754	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
nucleic acid binding	1.16049201286103	bayes_pls_golite062009
transporter activity	1.11833481501707	bayes_pls_golite062009
transcription factor activity	1.10928254946881	bayes_pls_golite062009
protein kinase binding	1.06030596516506	bayes_pls_golite062009
ErbB-2 class receptor binding	1.02996109010965	bayes_pls_golite062009
transmembrane transporter activity	0.97575992765505	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.959360414036299	bayes_pls_golite062009
growth factor binding	0.86076618871384	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	0.835012681606991	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	0.835012681606991	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	0.832111648073981	bayes_pls_golite062009
MAP kinase activity	0.81943579965027	bayes_pls_golite062009
actin filament binding	0.794152600084	bayes_pls_golite062009
substrate-specific transporter activity	0.705905455086486	bayes_pls_golite062009
ligase activity	0.665445021164512	bayes_pls_golite062009
structural constituent of cytoskeleton	0.612883428421652	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
enzyme binding	0.46284745266176	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.450699332958421	bayes_pls_golite062009
protein kinase C activity	0.41587987561486	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
MAP kinase kinase activity	0.35402045013148	bayes_pls_golite062009
insulin receptor binding	0.266450215230107	bayes_pls_golite062009
protein complex binding	0.239339759110451	bayes_pls_golite062009
insulin receptor activity	0.237134032032089	bayes_pls_golite062009
SH2 domain binding	0.226760162354251	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase activity	0.20643152368029	bayes_pls_golite062009
transforming growth factor beta receptor activity	0.20643152368029	bayes_pls_golite062009
receptor binding	0.197402253061255	bayes_pls_golite062009
ion transmembrane transporter activity	0.148194101970458	bayes_pls_golite062009
peptide binding	0.118578378753757	bayes_pls_golite062009
transforming growth factor beta receptor activity, type I	0.0506751594607393	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2