Protein Overview: PDB1

General Information:
Name(s) found:	PDB1 / YBR221C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319698\|ref\|NP_009780.1\| E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria; Pdb1p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S46097 pyruvate dehydrogenase (lipoamide) (EC 1.2.4.1) beta chain precursor - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|585609\|sp\|P32473.2\|ODPB_YEAST RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; AltName: Full=Pyruvate dehydrogenase complex component E1 beta; Short=PDHE1-B; Flags: Precursor [NCBI NR] gi\|536613\|emb\|CAA85184.1\| PDB1 [Saccharomyces cerevisiae] [NCBI NR] gi\|51013415\|gb\|AAT93001.1\| YBR221C [Saccharomyces cerevisiae] [NCBI NR] Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Saccharomyces cerevisiae GN=PDB1 PE=1 SV=2 [Swiss-Prot] PDB1 Pyruvate dehydrogenase (lipoamide) beta chain precursor [mips] E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria [SGD] PDB1, Chr II from 665111-666211, reverse complement [yeast_orfs2] Chr II from 665111-666211, reverse complement [yeastorf3.fasta] Chr II from 665110-666210, reverse complement [yeastorf4.fasta] Chr II from 666208-665108, reverse complement [yeastorf5.fasta] (P32473) Pyruvate dehydrogenase E1 component beta subunit, mitochondrial precursor (EC 1.2.4.1) (PDHE1-B) [4932.FASTACCERV.txt] PDB1 SGDID:S0000425, Chr II from 666210-665110, reverse complement, Verified ORF [yeastflysequest.fasta] PDB1 SGDID:S0000425, Chr II from 666248-665148, reverse complement, Verified ORF [10112005cervSGD.fasta] E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria [Saccharomyces cerevisiae]gi\|51013415\|gb\|AAT93001.1\| YBR221C [Saccharomyces cerevisiae]gi\|536613\|emb\|CAA85184.1 [nrdb_11152004_Chlamydomonas] PDB1 SGDID:S000000425, Chr II from 666248-665148, reverse complement, Verified ORF, "E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Pyruvate dehydrogenase E1 component subunit beta, mitochondrial precursor (Pyruvate dehydrogenase complex component E1 beta) (PDHE1-B) [nr-271106-contam.fasta] E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria; Pdb1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PDB1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YBR221C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PDB1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PDB1 SGDID:S000000425, Chr II from 666253-665153, Genome Release 64-1-1, reverse complement, Verified ORF, "E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria" [yeast-030211-contam.fasta] PDB1 SGDID:S000000425, Chr II from 666253-665153, Genome Release 64-2-1, reverse complement, Verified ORF, "E1 beta subunit of the pyruvate dehydrogenase (PDH) complex; PDH is an evolutionarily conserved multi-protein complex found in mitochondria" [20141208_orf_trans.fasta] TR4114\|c0_g1_i1\|g.2947 ORF TR4114\|c0_g1_i1\|g.2947 TR4114\|c0_g1_i1\|m.2947 type:complete len:367 (+) TR4114\|c0_g1_i1:207-1307(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YBR221C SGDID:S000000425, Chr II from 666253-665153, Genome Release 64-1-1, reverse complement, Verified ORF, "E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_5123\|g.3533 ORF asmbl_5123\|g.3533 asmbl_5123\|m.3533 type:complete len:367 (+) asmbl_5123:235-1335(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_5127\|g.3085 ORF ID=asmbl_5127\|g.3085 ID=asmbl_5127\|m.3085 type:complete len:367 (+) ID=asmbl_5127:235-1335(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	366 amino acids
Gene Ontology:
Cellular Component:	mitochondrial pyruvate dehydrogenase complex [TAS ] mitochondrion [IDA ][IMP] mitochondrial nucleoid [IDA]
Biological Process:	pyruvate metabolic process [NAS]
Molecular Function:	pyruvate dehydrogenase (acetyl-transferring) activity [IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFSRLPTSLA RNVARRAPTS FVRPSAAAAA LRFSSTKTMT VREALNSAMA EELDRDDDVF  60
   61 LIGEEVAQYN GAYKVSKGLL DRFGERRVVD TPITEYGFTG LAVGAALKGL KPIVEFMSFN 120
  121 FSMQAIDHVV NSAAKTHYMS GGTQKCQMVF RGPNGAAVGV GAQHSQDFSP WYGSIPGLKV 180
  181 LVPYSAEDAR GLLKAAIRDP NPVVFLENEL LYGESFEISE EALSPEFTLP YKAKIEREGT 240
  241 DISIVTYTRN VQFSLEAAEI LQKKYGVSAE VINLRSIRPL DTEAIIKTVK KTNHLITVES 300
  301 TFPSFGVGAE IVAQVMESEA FDYLDAPIQR VTGADVPTPY AKELEDFAFP DTPTIVKAVK 360
  361 EVLSIE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		LAT1 LPD1 PDA1 PDB1
View Details	(MIPS) Mewes HW, et al. (2004)		LAT1 LPD1 PDA1 PDB1 PDX1
View Details	Krogan NJ, et al. (2006)		ADY3 AIM23 PDB1
View Details	Gavin AC, et al. (2006)		LAT1 PDA1 PDB1 PDX1
View Details	Gavin AC, et al. (2002)		APM1 AVO1 BEM2 BLM10 CEG1 CET1 CLU1 CTR9 CYM1 DAM1 DBP9 DOA4 DUR1,2 ECM29 EST3 GFA1 HHF1, HHF2 HSP42 INO4 KAP95 LAT1 LPD1 LSM12 LYS12 MDS3 NIP1 NOP1 NTE1 NUD1 PDA1 PDB1 PDX1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE8 PRE9 PRP43 PRP46 PSE1 PUP3 RGR1 RPG1 RPN11 RPT3 RPT5 RRP12 SCL1 SLX9 SPA2 SPT16 SRP1 SSA3 SSA4 TCM62 TOP2 ULP1 VPS1 YFL006W YMR310C YRA1
View Details	Qiu et al. (2008)		KGD1 LPD1 PDA1 PDB1 PDX1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	SEC10	No Comments	De Craene, J., et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	MIH1	experimental2 - mih1-2	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	VAM3	sample: hx3	Hao Xu, et al. (2010)
View Run	VAM3	sample: hx4	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX13 - trans-SNARE complex forms but fusion is blocked.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	PDB1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..231]	PSI-BLAST	626.855515	E1-beta subunit of pyruvate dehydrogenase, Pyr module; E1-beta subunit of pyruvate dehydrogenase, C-domain
2	View Details	[232..366]	PSI-BLAST	626.855515	E1-beta subunit of pyruvate dehydrogenase, Pyr module; E1-beta subunit of pyruvate dehydrogenase, C-domain

Protein Overview: PDB1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: