Protein Overview: DOA4

General Information:
Name(s) found:	DOA4 / YDR069C [SGD]
Description(s) found: Found 33 descriptions. SHOW ALL	Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole [SGD]
Organism:	Saccharomyces cerevisiae
Length:	926 amino acids
Gene Ontology:
Cellular Component:	membrane fraction [IDA] endosome [IDA] mitochondrion [IDA ] proteasome complex [IPI]
Biological Process:	protein deubiquitination [TAS] telomere maintenance [IMP] regulation of DNA replication [IMP] endocytosis [IMP] ubiquitin-dependent protein catabolic process [IMP]
Molecular Function:	ubiquitin-specific protease activity [TAS] endopeptidase activity [IGI][IPI] ubiquitin thiolesterase activity [IDA][IMP] hydrolase activity [TAS ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEQNIISTIR DECIRHRSKY LTIAQLTAIA EAKINEFIIT GKAKDQDLSS LLDKCIDILS  60
   61 IYKKNSKDIK NIISCKNKGA MISSNSVMII QLNYVYYKVI HIIVTTNIPH LSEFAKIKLH 120
  121 KSTSDEGNGN NNNNEFQLMN IYNTLLETLL KDENIAKIKS FIKSSIKQTK LNHEQEECNL 180
  181 MRTGSYITSN QLNSLISSSA NSASSQMEIL LIDIRSRLEF NKSHIDTKNI ICLEPISFKM 240
  241 SYSDHDLEKK SLITSPNSEI KMFQSRNLFK FIILYTDANE YNVKQQSVLL DILVNHSFEK 300
  301 PISDDFTKIF ILESGFPGWL KSNYGRQVSS SFPSNNNIKD DSVYINGNTS GLSLQHLPKM 360
  361 SPSIRHSMDD SMKEMLVAPT PLNHLQQQQQ QQSDNDHVLK RSSSFKKLFS NYTSPNPKNS 420
  421 NSNLYSISSL SISSSPSPLP LHSPDPVKGN SLPINYPETP HLWKNSETDF MTNQREQLNH 480
  481 NSFAHIAPIN TKAITSPSRT ATPKLQRFPQ TISMNLNMNS NGHSSATSTI QPSCLSLSNN 540
  541 DSLDHTDVTP TSSHNYDLDF AVGLENLGNS CYMNCIIQCI LGTHELTQIF LDDSYAKHIN 600
  601 INSKLGSKGI LAKYFARLVH MMYKEQVDGS KKISISPIKF KLACGSVNSL FKTASQQDCQ 660
  661 EFCQFLLDGL HEDLNQCGSN PPLKELSQEA EARREKLSLR IASSIEWERF LTTDFSVIVD 720
  721 LFQGQYASRL KCKVCSHTST TYQPFTVLSI PIPKKNSRNN ITIEDCFREF TKCENLEVDE 780
  781 QWLCPHCEKR QPSTKQLTIT RLPRNLIVHL KRFDNLLNKN NDFVIYPFLL DLTPFWANDF 840
  841 DGVFPPGVND DELPIRGQIP PFKYELYGVA CHFGTLYGGH YTAYVKKGLK KGWLYFDDTK 900
  901 YKPVKNKADA INSNAYVLFY HRVYGV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		DOA4 RPN4 YTA7
View Details	Krogan NJ, et al. (2006)		AVT1 DOA4 MPC54 YEF1
View Details	Gavin AC, et al. (2006)		DOA4 DUR1,2
View Details	Gavin AC, et al. (2002)		APM1 BRE2 CYM1 DOA4 DUR1,2 HSP42 LAT1 LPD1 PDA1 PDB1 PDX1 PRP46 PRX1 RPN11 RSM7 SDC1 SET1 SPP1 SSZ1 SWD1 SWD3 TCM62
View Details	Qiu et al. (2008)		DOA4 UBP6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
transcription regulator activity	5.29569167054278	bayes_pls_golite062009
DNA binding	4.90756541371868	bayes_pls_golite062009
nucleic acid binding	4.69183445184264	bayes_pls_golite062009
transcription factor activity	4.14166958134441	bayes_pls_golite062009
binding	3.52874320222492	bayes_pls_golite062009
sequence-specific DNA binding	2.48053910222825	bayes_pls_golite062009
hydrolase activity	2.30467124306022	bayes_pls_golite062009
transcription activator activity	2.29614125067822	bayes_pls_golite062009
transcription repressor activity	2.19033875364989	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.48055533524841	bayes_pls_golite062009
small conjugating protein ligase activity	1.27763700765562	bayes_pls_golite062009
ubiquitin-protein ligase activity	1.12147035668008	bayes_pls_golite062009
transcription factor binding	0.83705247708153	bayes_pls_golite062009
acid-amino acid ligase activity	0.754042916649094	bayes_pls_golite062009
protein binding	0.753698599635855	bayes_pls_golite062009
structure-specific DNA binding	0.379762425417023	bayes_pls_golite062009
structural molecule activity	0.272316540117209	bayes_pls_golite062009
signal transducer activity	0.120341737642948	bayes_pls_golite062009
molecular transducer activity	0.120341737642948	bayes_pls_golite062009
structural constituent of ribosome	0.0912115683429473	bayes_pls_golite062009

No functions predicted.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.94

Source: Reynolds et al. (2008)

	PROTEIN(S)	PUBLICATION
View Data	DOA4 and SNF7	Huh WK, et al. (2003)
View Data	DOA4	Huh WK, et al. (2003)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..184]	deduced	N/A	No confident structure predictions are available.
2	View Details	[185..335]	PSI-BLAST	14.0	Adipocyte-transcription factor FREAC-11 (s12, fkh-14)
3	View Details	[336..561]	ORFEUS	7.33	No description for 1ldvA was found.
4	View Details	[562..753]	MSA	3.22999	View MSA. No confident structure predictions are available.
5	View Details	[754..926]	MSA	12.214985	View MSA. No confident structure predictions are available.

Protein Overview: DOA4

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: