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Protein Overview: POL1

General Information:
Name(s) found: POL1 / YNL102W [SGD]
Description(s) found:
Found 24 descriptions.
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  • Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1468 amino acids

    Gene Ontology:
    Cellular Component: replication fork [IDALink to PubMed article]
    alpha DNA polymerase:primase complex [IDALink to PubMed article]
    mitochondrion [IDALink to PubMed article]
    Biological Process: DNA replication initiation [ICLink to PubMed article]
    lagging strand elongation [ICLink to PubMed article]
    DNA replication [IMPLink to PubMed article]
    DNA synthesis involved in DNA repair [IMPLink to PubMed articleLink to PubMed article]
    premeiotic DNA synthesis [IMPLink to PubMed article]
    Molecular Function: nucleic acid binding [IEA]
    nucleotide binding [IEA]
    DNA binding [IEA]
    DNA-directed DNA polymerase activity [IDA][IEA]
    nucleotidyltransferase activity [IEA]
    transferase activity [IEA]
    nucleoside binding [IEA]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSKSEKLEK LRKLQAARNG TSIDDYEGDE SDGDRIYDEI DEKEYRARKR QELLHDDFVV  60
   61 DDDGVGYVDR GVEEDWREVD NSSSDEDTGN LASKDSKRKK NIKREKDHQI TDMLRTQHSK 120
  121 STLLAHAKKS QKKSIPIDNF DDILGEFESG EVEKPNILLP SKLRENLNSS PTSEFKSSIK 180
  181 RVNGNDESSH DAGISKKVKI DPDSSTDKYL EIESSPLKLQ SRKLRYANDV QDLLDDVENS 240
  241 PVVATKRQNV LQDTLLANPP SAQSLADEED DEDSDEDIIL KRRTMRSVTT TRRVNIDSRS 300
  301 NPSTSPFVTA PGTPIGIKGL TPSKSLQSNT DVATLAVNVK KEDVVDPETD TFQMFWLDYC 360
  361 EVNNTLILFG KVKLKDDNCV SAMVQINGLC RELFFLPREG KTPTDIHEEI IPLLMDKYGL 420
  421 DNIRAKPQKM KYSFELPDIP SESDYLKVLL PYQTPKSSRD TIPSDLSSDT FYHVFGGNSN 480
  481 IFESFVIQNR IMGPCWLDIK GADFNSIRNA SHCAVEVSVD KPQNITPTTT KTMPNLRCLS 540
  541 LSIQTLMNPK ENKQEIVSIT LSAYRNISLD SPIPENIKPD DLCTLVRPPQ STSFPLGLAA 600
  601 LAKQKLPGRV RLFNNEKAML SCFCAMLKVE DPDVIIGHRL QNVYLDVLAH RMHDLNIPTF 660
  661 SSIGRRLRRT WPEKFGRGNS NMNHFFISDI CSGRLICDIA NEMGQSLTPK CQSWDLSEMY 720
  721 QVTCEKEHKP LDIDYQNPQY QNDVNSMTMA LQENITNCMI SAEVSYRIQL LTLTKQLTNL 780
  781 AGNAWAQTLG GTRAGRNEYI LLHEFSRNGF IVPDKEGNRS RAQKQRQNEE NADAPVNSKK 840
  841 AKYQGGLVFE PEKGLHKNYV LVMDFNSLYP SIIQEFNICF TTVDRNKEDI DELPSVPPSE 900
  901 VDQGVLPRLL ANLVDRRREV KKVMKTETDP HKRVQCDIRQ QALKLTANSM YGCLGYVNSR 960
  961 FYAKPLAMLV TNKGREILMN TRQLAESMNL LVVYGDTDSV MIDTGCDNYA DAIKIGLGFK1020
 1021 RLVNERYRLL EIDIDNVFKK LLLHAKKKYA ALTVNLDKNG NGTTVLEVKG LDMKRREFCP1080
 1081 LSRDVSIHVL NTILSDKDPE EALQEVYDYL EDIRIKVETN NIRIDKYKIN MKLSKDPKAY1140
 1141 PGGKNMPAVQ VALRMRKAGR VVKAGSVITF VITKQDEIDN AADTPALSVA ERAHALNEVM1200
 1201 IKSNNLIPDP QYYLEKQIFA PVERLLERID SFNVVRLSEA LGLDSKKYFR REGGNNNGED1260
 1261 INNLQPLETT ITDVERFKDT VTLELSCPSC DKRFPFGGIV SSNYYRVSYN GLQCKHCEQL1320
 1321 FTPLQLTSQI EHSIRAHISL YYAGWLQCDD STCGIVTRQV SVFGKRCLND GCTGVMRYKY1380
 1381 SDKQLYNQLL YFDSLFDCEK NKKQELKPIY LPDDLDYPKE QLTESSIKAL TEQNRELMET1440
 1441 GRSVVQKYLN DCGRRYVDMT SIFDFMLN

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) POL1 POL12 PRI1 PRI2
    View Details (MIPS) Mewes HW, et al. (2004) POL1 POL12 PRI1 PRI2
    View Details Krogan NJ, et al. (2006) POL1 POL12 PRI1
    View Details Gavin AC, et al. (2006) POL1 POL12 PRI1 PRI2
    View Details Gavin AC, et al. (2002) AHA1 ATP11 CAF130 CAF40 CCR4 CCT6 CDC36 CDC39 COP1 FAS2 GCN1 MIS1 MOT2 NOT3 NOT5 PDC1 POL1 POL12 POP2 PRI1 PRI2 RFC1 RFC3 RFC4 RFC5 RVB2 SAM1 SEC27 TFC7 TFP1 USO1 YRA1
    View Details Qiu et al. (2008) DPB2 DPB3 DPB4 MCM3 MCM4 MCM6 POL1 POL12 POL2 POL3 POL30 POL31 POL32 PRI1 PRI2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen MET30 POL1 1 Unpublished Fields Lab Data
    View Screen UBC7 POL1 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data POL1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..338] MSA 4.071997 View MSA. No confident structure predictions are available.
    2 View Details [339..390]
    [479..813]    		 PSI-BLAST 1450.0 Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
    3 View Details [391..478]
    [814..846]
    [867..975]    		 PSI-BLAST 1450.0 Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
    4 View Details [847..866]
    [976..1069]    		 PSI-BLAST 1450.0 Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
    5 View Details [1070..1299] PSI-BLAST 1450.0 Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
    6 View Details [1300..1468] deduced N/A Confident ab initio structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • DNA primase activity
    		•  8.57378684936335 bayes_pls_golite062009
  • DNA-directed DNA polymerase activity
    		•  6.156872069104 bayes_pls_golite062009
  • DNA polymerase activity
    		•  6.10801780003354 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  4.59418010473269 bayes_pls_golite062009
  • ribonuclease activity
    		•  2.10426652514018 bayes_pls_golite062009
  • exonuclease activity
    		•  1.68989335095363 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.62213678119562 bayes_pls_golite062009
  • RNA polymerase activity
    		•  1.60828797652808 bayes_pls_golite062009
  • DNA-directed RNA polymerase activity
    		•  1.60828797652808 bayes_pls_golite062009
  • DNA binding
    		•  1.55767764828811 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  1.44884785815263 bayes_pls_golite062009
  • binding
    		•  1.23825244840872 bayes_pls_golite062009
  • damaged DNA binding
    		•  1.08077297289305 bayes_pls_golite062009
  • catalytic activity
    		•  1.04899053677564 bayes_pls_golite062009
  • endonuclease activity
    		•  0.991569665925888 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.863544378011356 bayes_pls_golite062009
  • exoribonuclease activity
    		•  0.85780161294346 bayes_pls_golite062009
  • 3'-5' exonuclease activity
    		•  0.78558451404229 bayes_pls_golite062009
  • transferase activity
    		•  0.784455099927681 bayes_pls_golite062009
  • exoribonuclease activity, producing 5'-phosphomonoesters
    		•  0.78013028675591 bayes_pls_golite062009
  • endodeoxyribonuclease activity
    		•  0.48776342704945 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  0.433956339585542 bayes_pls_golite062009
  • 3'-5'-exoribonuclease activity
    		•  0.43313823287883 bayes_pls_golite062009
  • chromatin binding
    		•  0.382725239240319 bayes_pls_golite062009
  • protein binding
    		•  0.377121823388117 bayes_pls_golite062009
  • nuclease activity
    		•  0.332024848299731 bayes_pls_golite062009
  • DNA helicase activity
    		•  0.283883977748512 bayes_pls_golite062009
  • endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  0.262305398570872 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  0.206750180223289 bayes_pls_golite062009
  • single-stranded DNA specific exodeoxyribonuclease activity
    		•  0.19596408995795 bayes_pls_golite062009
  • endoribonuclease activity, producing 5'-phosphomonoesters
    		•  0.193854882336037 bayes_pls_golite062009
  • 5'-3' exonuclease activity
    		•  0.154583332107704 bayes_pls_golite062009
  • transcription factor activity
    		•  0.093362945686819 bayes_pls_golite062009
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle