Protein Overview: TOP2

General Information:
Name(s) found:	TOP2 / YNL088W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|887623\|emb\|CAA61422.1\| ORF N2244 [Saccharomyces cerevisiae] [NCBI NR] gi\|6324241\|ref\|NP_014311.1\| Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores [NCBI NR] pir\|\|ISBYT2 DNA topoisomerase (ATP-hydrolyzing) (EC 5.99.1.3) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|19880887\|gb\|AAM00536.1\|AF458972_3 TOP2 [Saccharomyces cerevisiae] [NCBI NR] gi\|19880880\|gb\|AAM00530.1\|AF458971_3 TOP2 [Saccharomyces cerevisiae] [NCBI NR] gi\|19880866\|gb\|AAM00518.1\|AF458969_3 TOP2 [Saccharomyces cerevisiae] [NCBI NR] gi\|1351262\|sp\|P06786.2\|TOP2_YEAST RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II [NCBI NR] gi\|1301989\|emb\|CAA95964.1\| TOP2 [Saccharomyces cerevisiae] [NCBI NR] DNA topoisomerase 2 OS=Saccharomyces cerevisiae GN=TOP2 PE=1 SV=2 [Swiss-Prot] TOP2 DNA topoisomerase II (ATP-hydrolysing) [mips] Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis [SGD] TOP2, Chr XIV from 457701-461987 [yeast_orfs2] Chr XIV from 457701-461987 [yeastorf3.fasta] (P06786) DNA topoisomerase II (EC 5.99.1.3) [4932.FASTACCERV.txt] TOP2 SGDID:S0005032, Chr XIV from 457704-461990, Verified ORF [yeastflysequest.fasta] Essential type II topoisomerase, catalyzes topology changes in DNA via transient breakage and rejoining of phosphodiester bonds in the DNA backbone; localizes to axial cores in meiosis [Saccharomyces cerevisiae]gi\|19880887\|gb\|AAM00536.1\| TOP2 [Saccharomy [nrdb_11152004_Chlamydomonas] TOP2 SGDID:S000005032, Chr XIV from 457706-461992, Verified ORF, "Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complemen [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] TOP2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA topoisomerase 2 (DNA topoisomerase II) [nr-271106-contam.fasta] ORF N2244 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; Top2p [Saccharo [nr-271106-contam.fasta] TOP2 SGDID:S000005032, Chr XIV from 457706-461992, Verified ORF, "Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis" [yeast-bovrabprots.fasta] [chemostatdb.fasta] DNA topoisomerase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TOP2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] TOP2 SGDID:S000005032, Chr XIV from 457704-461990, Genome Release 64-1-1, Verified ORF, "Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis" [yeast-030211-contam.fasta] TOP2 SGDID:S000005032, Chr XIV from 457704-461990, Genome Release 64-2-1, Verified ORF, "Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation" [20141208_orf_trans.fasta] YNL088W SGDID:S000005032, Chr XIV from 457704-461990, Genome Release 64-1-1, Verified ORF, "Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1428 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] mitochondrion [IDA] synaptonemal complex [IDA]
Biological Process:	reciprocal meiotic recombination [IMP] DNA strand elongation involved in DNA replication [IMP] regulation of mitotic recombination [IMP] chromatin assembly or disassembly [IMP] DNA topological change [IDA]
Molecular Function:	DNA topoisomerase (ATP-hydrolyzing) activity [IDA ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTEPVSASD KYQKISQLEH ILKRPDTYIG SVETQEQLQW IYDEETDCMI EKNVTIVPGL  60
   61 FKIFDEILVN AADNKVRDPS MKRIDVNIHA EEHTIEVKND GKGIPIEIHN KENIYIPEMI 120
  121 FGHLLTSSNY DDDEKKVTGG RNGYGAKLCN IFSTEFILET ADLNVGQKYV QKWENNMSIC 180
  181 HPPKITSYKK GPSYTKVTFK PDLTRFGMKE LDNDILGVMR RRVYDINGSV RDINVYLNGK 240
  241 SLKIRNFKNY VELYLKSLEK KRQLDNGEDG AAKSDIPTIL YERINNRWEV AFAVSDISFQ 300
  301 QISFVNSIAT TMGGTHVNYI TDQIVKKISE ILKKKKKKSV KSFQIKNNMF IFINCLIENP 360
  361 AFTSQTKEQL TTRVKDFGSR CEIPLEYINK IMKTDLATRM FEIADANEEN ALKKSDGTRK 420
  421 SRITNYPKLE DANKAGTKEG YKCTLVLTEG DSALSLAVAG LAVVGRDYYG CYPLRGKMLN 480
  481 VREASADQIL KNAEIQAIKK IMGLQHRKKY EDTKSLRYGH LMIMTDQDHD GSHIKGLIIN 540
  541 FLESSFPGLL DIQGFLLEFI TPIIKVSITK PTKNTIAFYN MPDYEKWREE ESHKFTWKQK 600
  601 YYKGLGTSLA QEVREYFSNL DRHLKIFHSL QGNDKDYIDL AFSKKKADDR KEWLRQYEPG 660
  661 TVLDPTLKEI PISDFINKEL ILFSLADNIR SIPNVLDGFK PGQRKVLYGC FKKNLKSELK 720
  721 VAQLAPYVSE CTAYHHGEQS LAQTIIGLAQ NFVGSNNIYL LLPNGAFGTR ATGGKDAAAA 780
  781 RYIYTELNKL TRKIFHPADD PLYKYIQEDE KTVEPEWYLP ILPMILVNGA EGIGTGWSTY 840
  841 IPPFNPLEII KNIRHLMNDE ELEQMHPWFR GWTGTIEEIE PLRYRMYGRI EQIGDNVLEI 900
  901 TELPARTWTS TIKEYLLLGL SGNDKIKPWI KDMEEQHDDN IKFIITLSPE EMAKTRKIGF 960
  961 YERFKLISPI SLMNMVAFDP HGKIKKYNSV NEILSEFYYV RLEYYQKRKD HMSERLQWEV1020
 1021 EKYSFQVKFI KMIIEKELTV TNKPRNAIIQ ELENLGFPRF NKEGKPYYGS PNDEIAEQIN1080
 1081 DVKGATSDEE DEESSHEDTE NVINGPEELY GTYEYLLGMR IWSLTKERYQ KLLKQKQEKE1140
 1141 TELENLLKLS AKDIWNTDLK AFEVGYQEFL QRDAEARGGN VPNKGSKTKG KGKRKLVDDE1200
 1201 DYDPSKKNKK STARKGKKIK LEDKNFERIL LEQKLVTKSK APTKIKKEKT PSVSETKTEE1260
 1261 EENAPSSTSS SSIFDIKKED KDEGELSKIS NKFKKISTIF DKMGSTSATS KENTPEQDDV1320
 1321 ATKKNQTTAK KTAVKPKLAK KPVRKQQKVV ELSGESDLEI LDSYTDREDS NKDEDDAIPQ1380
 1381 RSRRQRSSRA ASVPKKSYVE TLELSDDSFI EDDEEENQGS DVSFNEED

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		FUN12 POB3 RPL15A RPL4B RPP0 RPS11A, RPS11B RPS1A RPS1B RPS3 RPS8B, RPS8A TOP2 UTP22 YLR455W
View Details	(MIPS) Mewes HW, et al. (2004)		HOP1 RED1 TOP1 TOP2 ZIP1 ZIP2
View Details	Krogan NJ, et al. (2006)		ABF2 ARG82 CKI1 CMR1 HHF1, HHF2 HTA1 HTA2 HTB1 HTB2 NUP188 PPH3 PSY2 PSY4 RRD1 SLX5 SLX8 SPT5 STP1 TOP2
View Details	Gavin AC, et al. (2002)		AVO1 BEM2 CEG1 CET1 CLU1 CTF18 CTF8 CTR9 DPB2 ELG1 HHF1, HHF2 LSM12 NIP1 NOP1 OLA1 PDB1 POL2 PRP43 RFC1 RFC2 RFC3 RFC4 RFC5 RPG1 SPT16 SSA3 SSA4 TOP2 VPS1 VPS13 YMR181C
View Details	Ho Y, et al. (2002)		CDC10 CDC11 CDC12 CDC3 CKA2 CKB1 CLU1 DUN1 LSP1 NOP56 PIL1 RNH203 RPN1 TOP2 YRA1 YTA6
View Details	Qiu et al. (2008)		HOP1 RED1 TOP2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	KAP104	TOP2	1	Unpublished Fields Lab Data
View Screen	RAD6	TOP2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TOP2	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..248]	PSI-BLAST	302.218487	DNA gyrase B
2	View Details	[249..405]	PSI-BLAST	302.218487	DNA gyrase B
3	View Details	[406..561] [604..656]	PSI-BLAST	11000.0	DNA topoisomerase II, C-terminal fragment (residues 410-1202)
4	View Details	[562..603] [657..701] [792..803] [816..879] [970..1012] [1147..1165]	PSI-BLAST	11000.0	DNA topoisomerase II, C-terminal fragment (residues 410-1202)
5	View Details	[702..791] [804..815] [880..969] [1013..1146] [1166..1207]	PSI-BLAST	11000.0	DNA topoisomerase II, C-terminal fragment (residues 410-1202)
6	View Details	[1208..1428]	MSA	2.140981	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2