Protein Overview: SPP382

General Information:
Name(s) found:	SPP382 / YLR424W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|74644961\|sp\|Q06411.1\|SP382_YEAST RecName: Full=Pre-mRNA-splicing factor SPP382; AltName: Full=Suppressor of PRP38 protein 2; AltName: Full=Nineteen complex-related protein 1; Short=NTC-related protein 1; AltName: Full=CLF1 complex factor 8 [NCBI NR] gi\|664877\|gb\|AAB67507.1\| Ylr424wp [Saccharomyces cerevisiae] [NCBI NR] gi\|6323456\|ref\|NP_013528.1\| Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38- [NCBI NR] pir\|\|S53411 hypothetical protein YLR424w - yeast (Saccharomyces cerevisiae) [NCBI NR] Pre-mRNA-splicing factor SPP382 OS=Saccharomyces cerevisiae GN=SPP382 PE=1 SV=1 [Swiss-Prot] NTR1 Essential protein involved in spliceosome disassembly and present in native splicing complexes [mips] Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation [SGD] YLR424W, Chr XII from 973390-975516 [yeast_orfs2] Chr XII from 973390-975516 [yeastorf3.fasta] >Q06411 (Q06411) SIMILARITY NEAR N-terminus to retrovirus-related protease [4932.FASTACCERV.txt] YLR424W SGDID:S0004416, Chr XII from 973391-975517, Uncharacterized ORF [yeastflysequest.fasta] SPP382 SGDID:S0004416, Chr XII from 973391-975517, Uncharacterized ORF [10112005cervSGD.fasta] Essential protein present in native splicing complexes; identified as a suppressor of prp38-1, a spliceosomal maturation mutation; cold sensitive alleles accumulate pre-mRNA [Saccharomyces cerevisiae]gi\|664877\|gb\|AAB67507.1\| Ylr424wp [Saccharomyces cerev [nrdb_11152004_Chlamydomonas] (Q06411) Ylr424wp [cerv4932.FASTAC] YLR424W SGDID:S000004416, Chr XII from 973391-975517, Uncharacterized ORF, "Essential protein present in native splicing complexes; identified as a suppressor of prp38-1, a spliceosomal maturation mutation; cold sensitive alleles accumulate pre-mRNA" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SPP382 SGDID:S000004416, Chr XII from 973392-975518, Verified ORF, "Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the spl [yeast-contam.fasta] Ylr424wp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation; Spp382p [Sacchar [nr-271106-contam.fasta] SPP382 SGDID:S000004416, Chr XII from 973392-975518, Verified ORF, "Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Pre-mRNA-splicing factor SPP382 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPP382 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SPP382 SGDID:S000004416, Chr XII from 973395-975521, Genome Release 64-1-1, Verified ORF, "Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation" [yeast-030211-contam.fasta] SPP382 SGDID:S000004416, Chr XII from 973395-975521, Genome Release 64-2-1, Verified ORF, "Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation" [20141208_orf_trans.fasta] YLR424W SGDID:S000004416, Chr XII from 973395-975521, Genome Release 64-1-1, Verified ORF, "Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	708 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cytoplasm [IDA] mitochondrion [IDA ] spliceosomal complex [IDA]
Biological Process:	nuclear mRNA splicing, via spliceosome [IPI]
Molecular Function:	RNA binding [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEDSDSNTDK KFFFKKRRID SYNYSDEEDN NSSMNSDMTY TNDALKTSSG NAPTISKLTK  60
   61 TYGIGAKLLS SMGYVAGKGL GKDGSGITTP IETQSRPMHN AGLGMFSNTN SSNYHSENED 120
  121 YLSSEDEVVE GIEQVKFNKT STEVLGEALL NDSGDMTIVR TLRELRLAGV QLPESILKEL 180
  181 DPLNAVPKPK KDVVVEILQE LLGIEKSLEA IRQRTSPLEV QVKEYYGQER LLSELEVTLR 240
  241 DESKHVSLYD KIGAILKLSD DELIDRLTSC LLRKELLIEF DLDHLEKPND ILDELTQIIE 300
  301 LLAYRMDTTS KFLNRTQTTI FKVIYPKLKK FWEGFDMTKS KIDSAITLLL DFQQVLSFIG 360
  361 CKEHIMEEFV YPKLLQELDN WELHDEVDHV SPRIWVLDFM VLIDDKIKDT IVDKIEAKFF 420
  421 AYCKNWYHRE SFCITNSDII FIKELICERR YYKILCKEFL PKFLDELWER HNDPIYELED 480
  481 WKEKQEWKEK DSGFFYFMKK LRSYTHYFHP KQYELMMRGT FNNINKILYQ WHLYSTVEDL 540
  541 HKSKWWLNWL MNTVFEHSLP TEIELSEIRK SYNIFAMSHR YHLDKSTLDE DFDLRQGLRN 600
  601 LMETQVIDDI SQSEQEPTYT VQNIPLGKVS SSFKDVVEDY CLEKGYLISK IPNRYTQLPY 660
  661 GRDQDCIVPL FEIRNGKKKM EVALKHDILW VEDSSGTFKP IYLWALDL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BRR2 CLF1 CSN12 CUS1 CWC2 CWC22 CWC23 DIB1 HSH155 LEA1 LUC7 NTR2 PRP19 PRP31 PRP4 PRP43 PRP46 PRP6 PRP8 PRP9 RSE1 SMB1 SMD3 SNT309 SNU114 SNU71 SPP382 STO1 SYF1
View Details	Hazbun TR, et al. (2003)		BRR2 CDC40 CEF1 CLF1 CWC2 CWC22 CWC23 ECM2 ISY1 LEA1 NTR2 PRP19 PRP43 PRP45 PRP46 PRP8 SMB1 SMD1 SMD3 SMX3 SNT309 SNU114 SPP382 SYF1 SYF2 YJU2
View Details	Krogan NJ, et al. (2006)		CWC23 PRP43 SPP382 SQS1
View Details	Gavin AC, et al. (2006)		CWC23 SPP382
View Details	Gavin AC, et al. (2002)		AAR2 ASC1 BRR1 BUD31 CBC2 CDC33 CDC40 CEF1 CLF1 CSN12 CUS1 CWC2 CWC22 CWC23 DCP2 DHH1 DIB1 ECM2 ERB1 GCN2 HRT1 HSH155 HSH49 HTA1 IMG1 ISY1 KRS1 LEA1 LIN1 LSM1 LSM2 LSM3 LSM4 LSM5 LSM6 LSM7 LUC7 MRP7 MRPL28 MRPL3 MRPL35 MRPL38 MRPL4 MRPL8 MRPS5 MSL1 MUD1 NAM7 NAM8 NOG2 NOP1 NPL3 NUG1 PAT1 PRP11 PRP18 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP46 PRP6 PRP8 PRP9 RSE1 RVB2 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP381 SPP382 SQS1 SRB2 SRO9 STO1 SYF1 TIF4631 TIF4632 TOS4 UTP21 YEF3 YML6
View Details	Qiu et al. (2008)		AAR2 BRR1 BRR2 CBC2 CDC40 CEF1 CLF1 CUS1 CWC15 CWC2 CWC22 CWC23 CWC25 DHR2 DIB1 HSH155 ISY1 LEA1 LSM2 LSM3 LSM4 LSM5 LSM7 LUC7 MAK5 MSL1 MUD1 MUD2 NAM8 NTC20 NTR2 PRP11 PRP16 PRP18 PRP19 PRP2 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP46 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU71 SPP2 SPP381 SPP382 STO1 SUB2 SYF1 SYF2 YHC1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CWC2	No Comments	Hazbun TR, et al. (2003)
View Run	SPP382	No Comments	Hazbun TR, et al. (2003)
View Run	SPP382	No Comments	Hazbun TR, et al. (2003)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPP382	YBL010C	2	Hazbun TR, et al. (2003)
View Screen	SPP382	LIF1	2	Hazbun TR, et al. (2003)
View Screen	SPP382	YLR031W	2	Hazbun TR, et al. (2003)
View Screen	SPP382	PRP6	2	Hazbun TR, et al. (2003)
View Screen	SPP382	PRP43	2	Hazbun TR, et al. (2003)
View Screen	SPP382	NUP49	2	Hazbun TR, et al. (2003)
View Screen	SPP382	YOL036W	2	Hazbun TR, et al. (2003)
View Screen	SPP382	SPO21	2	Hazbun TR, et al. (2003)
View Screen	SPP382	YBR138C	2	Hazbun TR, et al. (2003)
View Screen	SPP382	PRP3	2	Hazbun TR, et al. (2003)
View Screen	SPP382	MLP2	2	Hazbun TR, et al. (2003)
View Screen	ATG12	SPP382	2	Unpublished Fields Lab Data
View Screen	SPP382	MCM10	2	Hazbun TR, et al. (2003)
View Screen	SPP382	SWC7	2	Hazbun TR, et al. (2003)
View Screen	PCF11	SPP382	2	Uetz P, et al. (2000)
View Screen	PCF11	SPP382	2	Unpublished Fields Lab Data
View Screen	SPP382	HGH1	2	Hazbun TR, et al. (2003)
View Screen	SPP382	MEI5	2	Hazbun TR, et al. (2003)
View Screen	SPP382	SSK22	2	Hazbun TR, et al. (2003)
View Screen	SPP382	RDS2	2	Hazbun TR, et al. (2003)
View Screen	SWC4	SPP382	2	Hazbun TR, et al. (2003)
View Screen	SPP382	PAT1	2	Hazbun TR, et al. (2003)
View Screen	SPP382	LCP5	2	Hazbun TR, et al. (2003)
View Screen	SPP382	NIP100	2	Hazbun TR, et al. (2003)
View Screen	SPP382	CSM1	2	Hazbun TR, et al. (2003)
View Screen	SPP382	SPB4	2	Hazbun TR, et al. (2003)
View Screen	SPP382	PXR1	2	Hazbun TR, et al. (2003)
View Screen	SPP382	YJR056C	2	Hazbun TR, et al. (2003)
View Screen	SPP382	EPL1	2	Hazbun TR, et al. (2003)
View Screen	SPP382	PRP40	2	Hazbun TR, et al. (2003)
View Screen	SPP382	SLX5	2	Hazbun TR, et al. (2003)
View Screen	SPP382	PRP45	2	Hazbun TR, et al. (2003)
View Screen	SPP382	IOC3	2	Hazbun TR, et al. (2003)
View Screen	SPP382	RSC30	2	Hazbun TR, et al. (2003)
View Screen	SPP382	NTR2	2	Uetz P, et al. (2000)
View Screen	SPP382	MTW1	2	Hazbun TR, et al. (2003)
View Screen	SPP382	ADY3	2	Hazbun TR, et al. (2003)
View Screen	SPP382	DAL80	2	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SPP382 and SPC42	Unpublished Data
View Data	SPP382	Unpublished Data
View Data	SPP382	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..111]	Pfam	15.585027	G-patch domain No confident structure predictions are available.
2	View Details	[112..193]	deduced	N/A	No confident structure predictions are available.
3	View Details	[194..708]	MSA	1.016866	View MSA. No confident structure predictions are available.
4	View Details	[560..708]	MSA	1.041961	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2