Protein Overview: EBP2

General Information:
Name(s) found:	EBP2 / YKL172W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322676\|ref\|NP_012749.1\| Essential protein required for the maturation of 25S rRNA and 60S ribosomal subunit assembly, localizes to the nucleolus; constituent of 66S pre-ribosomal particles; Ebp2p [Saccharomyces cerevisiae] [NCBI NR] gi\|549736\|sp\|P36049.1\|EBP2_YEAST RecName: Full=rRNA-processing protein EBP2; AltName: Full=EBNA1-binding protein homolog [NCBI NR] pir\|\|S38002 hypothetical protein YKL172w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|486302\|emb\|CAA82014.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|407510\|emb\|CAA81515.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|1582533\|prf\|\|2118403G ORF [NCBI NR] rRNA-processing protein EBP2 OS=Saccharomyces cerevisiae GN=EBP2 PE=1 SV=1 [Swiss-Prot] EBP2 Protein required for pre-rRNA processing and ribosomal subunit assembly [mips] Essential protein required for the maturation of 25S rRNA and 60S ribosomal subunit assembly, localizes to the nucleolus; constituent of 66S pre-ribosomal particles [SGD] EBP2, Chr XI from 125761-127044 [yeast_orfs2] Chr XI from 125761-127044 [yeastorf3.fasta] Chr XI from 125760-127043 [yeastorf4.fasta] (P36049) rRNA processing protein EBP2 (EBNA1-binding protein homolog) [4932.FASTACCERV.txt] EBP2 SGDID:S0001655, Chr XI from 125764-127047, Verified ORF [yeastflysequest.fasta] Ebp2p [Saccharomyces cerevisiae]gi\|407510\|emb\|CAA81515.1\| unknown [Saccharomyces cerevisiae]gi\|486302\|emb\|CAA82014.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|539212\|pir\|\|S38002 hypothetical protein YKL172w - yeast (Saccharomyces cerevisiae [nrdb_11152004_Chlamydomonas] EBP2 SGDID:S000001655, Chr XI from 125764-127047, Verified ORF, "Essential protein required for the maturation of 25S rRNA and 60S ribosomal subunit assembly, localizes to the nucleolus; constituent of 66S pre-ribosomal particles" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Essential protein required for the maturation of 25S rRNA and 60S ribosomal subunit assembly, localizes to the nucleolus; constituent of 66S pre-ribosomal particles; Ebp2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ORF [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] rRNA-processing protein EBP2 (EBNA1-binding protein homolog) [nr-271106-contam.fasta] [chemostatdb.fasta] rRNA-processing protein EBP2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EBP2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] EBP2 SGDID:S000001655, Chr XI from 125759-127042, Genome Release 64-1-1, Verified ORF, "Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering" [yeast-030211-contam.fasta] EBP2 SGDID:S000001655, Chr XI from 125759-127042, Genome Release 64-2-1, Verified ORF, "Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering" [20141208_orf_trans.fasta] YKL172W SGDID:S000001655, Chr XI from 125759-127042, Genome Release 64-1-1, Verified ORF, "Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	427 amino acids
Gene Ontology:
Cellular Component:	nucleolus [IDA]
Biological Process:	nuclear division [IMP] ribosome biogenesis [RCA] rRNA processing [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAKGFKLKEL LSHQKEIEKA EKLENDLKKK KSQELKKEEP TIVTASNLKK LEKKEKKADV  60
   61 KKEVAADTEE YQSQALSKKE KRKLKKELKK MQEQDATEAQ KHMSGDEDES GDDREEEEEE 120
  121 EEEEEGRLDL EKLAKSDSES EDDSESENDS EEDEDVVAKE ESEEKEEQEE EQDVPLSDVE 180
  181 FDSDADVVPH HKLTVNNTKA MKHALERVQL PWKKHSFQEH QSVTSETNTD EHIKDIYDDT 240
  241 ERELAFYKQS LDAVLVARDE LKRLKVPFKR PLDYFAEMVK SDEHMDKIKG KLIEEASDKK 300
  301 AREEARRQRQ LKKFGKQVQN ATLQKRQLEK RETLEKIKSL KNKRKHNEID HSEFNVGVEE 360
  361 EVEGKRFDRG RPNGKRAAKN AKYGQGGMKR FKRKNDATSS ADVSGFSSRK MKGKTNRPGK 420
  421 SRRARRF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BRX1 CIC1 DBP10 DBP9 DRS1 EBP2 ERB1 LOC1 MAK21 NIP7 NOC2 NOC3 NOG1 NOP12 NOP2 NOP53 NOP7 NSA1 NUG1 PUF6 PWP1 RLP7 RPF2 RPL15A RPL5 RPL8B YTM1
View Details	Krogan NJ, et al. (2006)		EBP2 PWP1
View Details	Gavin AC, et al. (2006)		CIC1 EBP2 IPI1 RPL23B, RPL23A RPL31B RTR1
View Details	Gavin AC, et al. (2002)		ARX1 BEM2 BMS1 BRX1 BUD20 CIC1 CLU1 CYR1 CYS4 DBP10 DBP9 DCP2 DRS1 EBP2 ERB1 FPR4 GCN1 HAS1 HDA1 HER1 HIS3 INP2 IPI1 IPI3 KRE33 LHP1 LOC1 LSG1 MAK21 MAK5 MDN1 MIS1 MRT4 NAP1 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP7 NSA1 NSA2 NUG1 PRT1 PUF6 REI1 RIX1 RIX7 RLP24 RLP7 RPA135 RPA190 RPF1 RPF2 RPG1 RPP2B RPT6 RRP1 RRP12 RSA3 RSA4 RTR1 SDA1 SEC7 SKI2 SNU13 SPB1 SPB4 SSF1 TIF6 TUB3 URB1 UTP10 UTP20 UTP22 YGL036W YTM1
View Details	Ho Y, et al. (2002)		BRX1 CIC1 DBP7 DRS1 EBP2 ELA1 ERB1 EXG1 FPR4 IMD2 IMD3 IMD4 KRI1 MAK5 MRT4 MSS116 NOC2 NOC3 NOG1 NOP1 NOP12 NOP13 NOP15 NOP16 NOP2 NOP4 NOP58 NOP7 PET127 PIB2 PUF6 PWP1 RLP7 RPF2 RRP12 RRP17 RRP5 SCS2 THI21 TIF4631 TIF6 TIS11 YER077C YTM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YJR012C	EBP2	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..6] [229..347]	ORFEUS	10.86	No description for 1l8mA was found.
2	View Details	[7..79]	ORFEUS	10.86	No description for 1l8mA was found.
3	View Details	[80..158]	ORFEUS	10.86	No description for 1l8mA was found.
4	View Details	[159..228]	ORFEUS	10.86	No description for 1l8mA was found.
5	View Details	[348..427]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2