Protein Overview: MRPL8

General Information:
Name(s) found:	MRPL8 / YJL063C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|730590\|sp\|P22353.4\|RM08_YEAST RecName: Full=54S ribosomal protein L8, mitochondrial; AltName: Full=YmL8 [NCBI NR] gi\|6322398\|ref\|NP_012472.1\| Mitochondrial ribosomal protein of the large subunit; Mrpl8p [Saccharomyces cerevisiae] [NCBI NR] gi\|499004\|emb\|CAA84060.1\| HRD238 [Saccharomyces cerevisiae] [NCBI NR] gi\|45270388\|gb\|AAS56575.1\| YJL063C [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S50809 ribosomal protein L17, mitochondrial - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1008211\|emb\|CAA89354.1\| MRPL8 [Saccharomyces cerevisiae] [NCBI NR] 54S ribosomal protein L8, mitochondrial OS=Saccharomyces cerevisiae GN=MRPL8 PE=1 SV=4 [Swiss-Prot] MRPL8 Mitochondrial ribosomal protein, large subunit [mips] Mitochondrial ribosomal protein of the large subunit [SGD] MRPL8, Chr X from 315455-316171, reverse complement [yeast_orfs2] Chr X from 315455-316171, reverse complement [yeastorf3.fasta] Chr X from 316171-315455, reverse complement [yeastorf5.fasta] (P22353) Mitochondrial 60S ribosomal protein L8 (YmL8) [4932.FASTACCERV.txt] MRPL8 SGDID:S0003599, Chr X from 316391-315675, reverse complement, Verified ORF [yeastflysequest.fasta] Mitochondrial ribosomal protein of the large subunit [Saccharomyces cerevisiae]gi\|1008211\|emb\|CAA89354.1\| MRPL8 [Saccharomyces cerevisiae]gi\|499004\|emb\|CAA84060.1\| HRD238 [Saccharomyces cerevisiae]gi\|45270388\|gb\|AAS56575.1\| YJL063C [Saccharomyces cerev [nrdb_11152004_Chlamydomonas] MRPL8 SGDID:S000003599, Chr X from 316391-315675, reverse complement, Verified ORF, "Mitochondrial ribosomal protein of the large subunit" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MRPL8 SGDID:S000003599, Chr X from 316470-315754, reverse complement, Verified ORF, "Mitochondrial ribosomal protein of the large subunit" [yeast-contam.fasta] MRPL8 SGDID:S000003599, Chr X from 316469-315753, reverse complement, Verified ORF, "Mitochondrial ribosomal protein of the large subunit" [yeast-200209-contam.fasta] YJL063C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] MRPL8 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mitochondrial ribosomal protein of the large subunit; Mrpl8p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] HRD238 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mitochondrial 60S ribosomal protein L8 (YmL8) [nr-271106-contam.fasta] [chemostatdb.fasta] 54S ribosomal protein L8, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MRPL8 PE=1 SV=4 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MRPL8 SGDID:S000003599, Chr X from 316475-315759, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondrial ribosomal protein of the large subunit" [yeast-030211-contam.fasta] MRPL8 SGDID:S000003599, Chr X from 316475-315759, Genome Release 64-2-1, reverse complement, Verified ORF, "Mitochondrial ribosomal protein of the large subunit" [20141208_orf_trans.fasta] TR609\|c0_g1_i1\|g.384 ORF TR609\|c0_g1_i1\|g.384 TR609\|c0_g1_i1\|m.384 type:complete len:239 (+) TR609\|c0_g1_i1:50-766(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YJL063C SGDID:S000003599, Chr X from 316475-315759, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondrial ribosomal protein of the large subunit" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_5307\|g.4136 ORF asmbl_5307\|g.4136 asmbl_5307\|m.4136 type:complete len:239 (+) asmbl_5307:57-773(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_5310\|g.3328 ORF ID=asmbl_5310\|g.3328 ID=asmbl_5310\|m.3328 type:complete len:239 (+) ID=asmbl_5310:57-773(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	238 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA] mitochondrial large ribosomal subunit [IPI][TAS]
Biological Process:	mitochondrial genome maintenance [IMP] translation [TAS]
Molecular Function:	structural constituent of ribosome [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTVGIARKLS RDKAHRDALL KNLACQLFQH ESIVSTHAKC KEASRVAERI ITWTKRAITT  60
   61 SNSVAQAELK SQIQSQLFLA GDNRKLMKRL FSEIAPRYLE RPGGYTRVLR LEPRANDSAP 120
  121 QSVLELVDSP VMSESHTVNR GNLKMWLLVK SVINDDANQL PHNPLTLQNL HKVAKFKAEA 180
  181 QLHGEIMLIK QVLLKEMSLP YDEALENERT QALLKEVYSS SLPKKTKKPS SYVMVPRP

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ATG26 IMG1 IMG2 MHR1 MRP20 MRP49 MRP7 MRPL1 MRPL13 MRPL15 MRPL16 MRPL17 MRPL20 MRPL22 MRPL23 MRPL24 MRPL28 MRPL3 MRPL35 MRPL36 MRPL4 MRPL51 MRPL7 MRPL8 MRPL9 RTC6 YML6
View Details	(MIPS) Mewes HW, et al. (2004)		IMG1 IMG2 MNP1 MRP20 MRP49 MRP7 MRPL1 MRPL10 MRPL11 MRPL13 MRPL15 MRPL16 MRPL17 MRPL19 MRPL20 MRPL22 MRPL23 MRPL24 MRPL25 MRPL27 MRPL28 MRPL3 MRPL31 MRPL32 MRPL33 MRPL35 MRPL36 MRPL37 MRPL38 MRPL39 MRPL4 MRPL40 MRPL44 MRPL49 MRPL50 MRPL51 MRPL6 MRPL7 MRPL8 MRPL9 RML2 RTC6 YDR115W YML6
View Details	Krogan NJ, et al. (2006)		CBF1 FYV4 MAM33 MRP1 MRP10 MRP17 MRP21 MRP4 MRP51 MRPL8 MRPS12 MRPS16 MRPS17 MRPS18 MRPS28 MRPS35 MRPS5 MRPS9 NAM9 NCL1 PET123 RIB4 RSM10 RSM18 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM28 RSM7 SVL3 SWS2 YDR124W YJR096W YKL151C YSP1
View Details	Gavin AC, et al. (2006)		IMG1 MHR1 MRP49 MRPL35 MRPL8 MRPL9 YML6
View Details	Gavin AC, et al. (2002)		AAR2 ATG26 BEM2 BRR1 BUD3 BUD31 CDC33 CEF1 CLF1 COP1 CSN12 CUS1 CWC2 CWC22 CWC23 CYR1 DHH1 DIB1 ECM2 GCN2 HRR25 HSH155 HTA1 IMG1 IMG2 IML1 LEA1 LIN1 LSM1 LSM2 LSM4 LSM5 LSM6 LSM7 LUC7 MHR1 MRH4 MRM1 MRP20 MRP49 MRP7 MRPL1 MRPL10 MRPL13 MRPL15 MRPL16 MRPL17 MRPL19 MRPL20 MRPL23 MRPL24 MRPL25 MRPL27 MRPL28 MRPL3 MRPL35 MRPL36 MRPL38 MRPL39 MRPL4 MRPL44 MRPL51 MRPL6 MRPL7 MRPL8 MRPS5 MSL1 MUD1 NIC96 NSP1 NUG1 NUP116 NUP159 NUP82 PAT1 PRP11 PRP18 PRP19 PRP21 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP46 PRP6 PRP8 PRP9 RET2 ROM2 RSE1 RTC6 RVB2 SEC1 SEC26 SEC27 SIN4 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNF2 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP381 SPP382 SRB2 STO1 SWI3 SYF1 SYP1 TAF5 TOS4 UTP20 UTP21 YDR115W YML6
View Details	Qiu et al. (2008)		EHD3 IMG1 IMG2 MAM33 MHR1 MNP1 MRP1 MRP10 MRP13 MRP17 MRP2 MRP20 MRP21 MRP4 MRP49 MRP51 MRP7 MRPL1 MRPL10 MRPL11 MRPL13 MRPL15 MRPL16 MRPL17 MRPL19 MRPL20 MRPL22 MRPL23 MRPL24 MRPL25 MRPL27 MRPL28 MRPL3 MRPL31 MRPL32 MRPL33 MRPL35 MRPL36 MRPL37 MRPL38 MRPL39 MRPL4 MRPL40 MRPL44 MRPL49 MRPL50 MRPL51 MRPL6 MRPL7 MRPL8 MRPL9 MRPS12 MRPS16 MRPS17 MRPS18 MRPS28 MRPS35 MRPS5 MRPS8 MRPS9 NAM9 PET123 RML2 RSM10 RSM18 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM7 RTC6 SWS2 YDR115W YML6 YMR31

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HNT2	MRPL8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MRPL8	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..161]	PSI-BLAST	162.38764	Prokaryotic ribosomal protein L17
2	View Details	[162..238]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
structural constituent of ribosome	9.91574154879999	bayes_pls_golite062009
structural molecule activity	9.33953790706737	bayes_pls_golite062009
binding	1.23500675594838	bayes_pls_golite062009
protein binding	0.558431147696839	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2