Protein Overview: NAM8

General Information:
Name(s) found:	NAM8 / YHR086W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|730108\|sp\|Q00539.2\|NAM8_YEAST RecName: Full=Protein NAM8 [NCBI NR] gi\|6321878\|ref\|NP_011954.1\| RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of MER2 pre-mRNA; Nam [NCBI NR] pir\|\|S46720 NAM8 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|520596\|dbj\|BAA02016.1\| Mre2 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|487934\|gb\|AAB68928.1\| Nam8p: Putative RNA binding proteins [Saccharomyces cerevisiae] [NCBI NR] Protein NAM8 OS=Saccharomyces cerevisiae GN=NAM8 PE=1 SV=2 [Swiss-Prot] NAM8 Meiotic recombination protein [mips] RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of MER2 pre-mRNA [SGD] NAM8, Chr VIII from 278154-279725 [yeast_orfs2] Chr VIII from 278154-279725 [yeastorf3.fasta] (Q00539) NAM8 protein [4932.FASTACCERV.txt] NAM8 SGDID:S0001128, Chr VIII from 278154-279725, Verified ORF [yeastflysequest.fasta] Nam8p [Saccharomyces cerevisiae]gi\|630031\|pir\|\|S46720 NAM8 protein - yeast (Saccharomyces cerevisiae)gi\|487934\|gb\|AAB68928.1\| Nam8p: Putative RNA binding proteins [Saccharomyces cerevisiae]gi\|520596\|dbj\|BAA02016.1\| Mre2 protein [Saccharomyces cerevisia [nrdb_11152004_Chlamydomonas] NAM8 SGDID:S000001128, Chr VIII from 278155-279726, Verified ORF, "RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis- [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein NAM8 [nr-271106-contam.fasta] RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of MER2 pre-mRNA; Nam8p [Saccharomyces cerevisiae [nr-271106-contam.fasta] Mre2 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Nam8p: Putative RNA binding proteins [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NAM8 SGDID:S000001128, Chr VIII from 278155-279726, Verified ORF, "RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of MER2 pre-mRNA" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Protein NAM8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NAM8 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NAM8 SGDID:S000001128, Chr VIII from 278153-279724, Genome Release 64-1-1, Verified ORF, "RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function" [yeast-030211-contam.fasta] NAM8 SGDID:S000001128, Chr VIII from 278153-279724, Genome Release 64-2-1, Verified ORF, "RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function" [20141208_orf_trans.fasta] YHR086W SGDID:S000001128, Chr VIII from 278153-279724, Genome Release 64-1-1, Verified ORF, "RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	523 amino acids
Gene Ontology:
Cellular Component:	commitment complex [IPI] U1 snRNP [IDA ]
Biological Process:	mRNA splice site selection [IMP] nuclear mRNA splicing, via spliceosome [IPI]
Molecular Function:	RNA binding [IPI] mRNA binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSYKQTTYYP SRGNLVRNDS SPYTNTISSE TNNSSTSVLS LQGASNVSLG TTGNQLYMGD  60
   61 LDPTWDKNTV RQIWASLGEA NINVRMMWNN TLNNGSRSSM GPKNNQGYCF VDFPSSTHAA 120
  121 NALLKNGMLI PNFPNKKLKL NWATSSYSNS NNSLNNVKSG NNCSIFVGDL APNVTESQLF 180
  181 ELFINRYAST SHAKIVHDQV TGMSKGYGFV KFTNSDEQQL ALSEMQGVFL NGRAIKVGPT 240
  241 SGQQQHVSGN NDYNRSSSSL NNENVDSRFL SKGQSFLSNG NNNMGFKRNH MSQFIYPVQQ 300
  301 QPSLNHFTDP NNTTVFIGGL SSLVTEDELR AYFQPFGTIV YVKIPVGKCC GFVQYVDRLS 360
  361 AEAAIAGMQG FPIANSRVRL SWGRSAKQTA LLQQAMLSNS LQVQQQQPGL QQPNYGYIPS 420
  421 STCEAPVLPD NNVSSTMLPG CQILNYSNPY ANANGLGSNN FSFYSNNNAT NTQATSLLAD 480
  481 TSSMDLSGTG GQQVIMQGSE AVVNSTNAML NRLEQGSNGF MFA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CBC2 LUC7 MUD1 NAM8 PRP39 PRP40 PRP42 RSE1 SMB1 SMD1 SMD2 SMD3 SNP1 SNU56 SNU71 STO1 YHC1
View Details	Krogan NJ, et al. (2006)		AAR2 BRR1 BRR2 CSE1 LUC7 MUD1 NAM8 PRP2 PRP3 PRP31 PRP39 PRP40 PRP42 PRP6 PRP8 RRG7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNU114 SNU56 SNU66 SNU71 TAD2 YDL085C-A YHC1 YJU2 YNL092W
View Details	Gavin AC, et al. (2006)		LUC7 MUD1 NAM8 PRP39 PRP40 PRP42 SNP1 SNU56 SNU71 YHC1
View Details	Gavin AC, et al. (2002)		AIR2 BRR1 BUR2 CBC2 CDC33 CLF1 CSN12 CUS1 CWC2 DIB1 ECM2 HSH155 HTA1 KAP95 LEA1 LSM4 LUC7 MSH4 MSL1 MUD1 NAB3 NAB6 NAM8 NPL3 NRD1 NUP60 PRP11 PRP19 PRP21 PRP3 PRP31 PRP39 PRP4 PRP40 PRP42 PRP43 PRP46 PRP6 PRP9 RSE1 SCP160 SEN1 SGV1 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU56 SNU66 SNU71 SPP382 SRB2 SRO9 SRP1 STO1 THO2 TIF4631 TIF4632 YEF3 YRA2
View Details	Ho Y, et al. (2002)		DIS3 EAF3 FIP1 HAS1 HPR1 KAP95 MES1 MFT1 NAM8 NOP6 NUP1 NUP2 NUP60 PAP1 PCT1 REB1 RNT1 RRP4 RRP43 RRP6 RTT103 SIF2 SIN3 SNU56 SRP1 STO1 THO2 TRA1 UME1 YPR090W
View Details	Qiu et al. (2008)		AAR2 BRR1 BRR2 CBC2 CDC40 CEF1 CLF1 CUS1 CUS2 CWC15 CWC2 CWC22 CWC23 CWC25 DHR2 DIB1 ECM2 HSH155 HSH49 IST3 ISY1 LEA1 LSM3 LSM4 LSM5 LUC7 MAK5 MSL1 MUD1 MUD2 NAM8 PRP11 PRP16 PRP18 PRP19 PRP2 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNU114 SNU23 SNU66 SPP2 SPP381 SPP382 STO1 SUB2 SYF2 YHC1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CLB2	No Comments	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PET111	NAM8	2	Unpublished Fields Lab Data
View Screen	GTS1	NAM8	2	Unpublished Fields Lab Data
View Screen	ARF3	NAM8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NAM8	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..164]	PSI-BLAST	126.67837	Poly(A)-binding protein
2	View Details	[165..303]	PSI-BLAST	14.522879	Polypyrimidine tract-binding protein
3	View Details	[304..396]	PSI-BLAST	14.522879	Polypyrimidine tract-binding protein
4	View Details	[397..523]	MSA	1.382997	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
RNA binding	6.73097473547076	bayes_pls_golite062009
	6.62953239642182	bayes_pls_golite062009
mRNA binding	5.29759606920196	bayes_pls_golite062009
nucleic acid binding	4.92126426323641	bayes_pls_golite062009
U1 snRNA binding	4.22094068431436	bayes_pls_golite062009
snRNA binding	4.06596327298973	bayes_pls_golite062009
binding	3.64400841800969	bayes_pls_golite062009
poly-pyrimidine tract binding	3.11990160533699	bayes_pls_golite062009
single-stranded RNA binding	2.93798024346416	bayes_pls_golite062009
structural molecule activity	2.78501986457503	bayes_pls_golite062009
protein binding	2.17260848225967	bayes_pls_golite062009
transcription regulator activity	1.92450390013691	bayes_pls_golite062009
DNA binding	1.8021593476478	bayes_pls_golite062009
transcription factor activity	1.15127506309683	bayes_pls_golite062009
transcription activator activity	1.12833244388568	bayes_pls_golite062009
transcription repressor activity	0.998305828880243	bayes_pls_golite062009
mRNA 3'-UTR binding	0.824614388276642	bayes_pls_golite062009
mRNA 5'-UTR binding	0.625848256054783	bayes_pls_golite062009
translation repressor activity, nucleic acid binding	0.461000905454505	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.425042944899091	bayes_pls_golite062009
hydrolase activity	0.389390547875764	bayes_pls_golite062009
sequence-specific DNA binding	0.383589644462932	bayes_pls_golite062009
translation regulator activity	0.38120065819101	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.128616879457381	bayes_pls_golite062009
snoRNA binding	0.0568636182001701	bayes_pls_golite062009

Term	Confidence	Notes
RNA binding	6.73097473547076	bayes_pls_golite062009
	6.62953239642182	bayes_pls_golite062009
mRNA binding	5.29759606920196	bayes_pls_golite062009
nucleic acid binding	4.92126426323641	bayes_pls_golite062009
U1 snRNA binding	4.22094068431436	bayes_pls_golite062009
snRNA binding	4.06596327298973	bayes_pls_golite062009
binding	3.64400841800969	bayes_pls_golite062009
poly-pyrimidine tract binding	3.11990160533699	bayes_pls_golite062009
single-stranded RNA binding	2.93798024346416	bayes_pls_golite062009
structural molecule activity	2.78501986457503	bayes_pls_golite062009
protein binding	2.17260848225967	bayes_pls_golite062009
transcription regulator activity	1.92450390013691	bayes_pls_golite062009
DNA binding	1.8021593476478	bayes_pls_golite062009
transcription factor activity	1.15127506309683	bayes_pls_golite062009
transcription activator activity	1.12833244388568	bayes_pls_golite062009
transcription repressor activity	0.998305828880243	bayes_pls_golite062009
mRNA 3'-UTR binding	0.824614388276642	bayes_pls_golite062009
mRNA 5'-UTR binding	0.625848256054783	bayes_pls_golite062009
translation repressor activity, nucleic acid binding	0.461000905454505	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.425042944899091	bayes_pls_golite062009
hydrolase activity	0.389390547875764	bayes_pls_golite062009
sequence-specific DNA binding	0.383589644462932	bayes_pls_golite062009
translation regulator activity	0.38120065819101	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.128616879457381	bayes_pls_golite062009
snoRNA binding	0.0568636182001701	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2