Protein Overview: PCF11

General Information:
Name(s) found:	PCF11 / YDR228C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|728684\|emb\|CAA88508.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6320434\|ref\|NP_010514.1\| mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA po [NCBI NR] gi\|2851505\|sp\|P39081.2\|PCF11_YEAST RecName: Full=Protein PCF11; AltName: Full=protein 1 of CF I [NCBI NR] pir\|\|S59435 hypothetical protein YDR228c - yeast (Saccharomyces cerevisiae) [NCBI NR] Protein PCF11 OS=Saccharomyces cerevisiae GN=PCF11 PE=1 SV=2 [Swiss-Prot] (P39081) PCF11 protein [Swiss-Prot] PCF11 Component of pre-mRNA 3`-end processing factor CF I [mips] mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p) [SGD] PCF11, Chr IV from 921917-923797, reverse complement [yeast_orfs2] Chr IV from 921917-923797, reverse complement [yeastorf3.fasta] Chr IV from 923797-921917, reverse complement [yeastorf5.fasta] PCF11 SGDID:S0002636, Chr IV from 923798-921918, reverse complement, Verified ORF [yeastflysequest.fasta] PCF11 SGDID:S0002636, Chr IV from 923800-921920, reverse complement, Verified ORF [10112005cervSGD.fasta] Pcf11p [Saccharomyces cerevisiae]gi\|728684\|emb\|CAA88508.1\| unknown [Saccharomyces cerevisiae]gi\|1363783\|pir\|\|S59435 hypothetical protein YDR228c - yeast (Saccharomyces cerevisiae)gi\|2851505\|sp\|P39081\|PC11_YEAST PCF11 PROTEIN [nrdb_11152004_Chlamydomonas] PCF11 SGDID:S000002636, Chr IV from 923800-921920, reverse complement, Verified ORF, "mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termina [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PCF11 SGDID:S000002636, Chr IV from 923802-921922, reverse complement, Verified ORF, "mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termina [yeast-contam.fasta] PCF11 SGDID:S000002636, Chr IV from 923803-921923, reverse complement, Verified ORF, "mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termina [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); Pcf11p [Sacch [nr-271106-contam.fasta] Protein PCF11 (protein 1 of CF I) [nr-271106-contam.fasta] PCF11 SGDID:S000002636, Chr IV from 923803-921923, reverse complement, Verified ORF, "mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p)" [yeast-bovrabprots.fasta] PCF11 SGDID:S000002636, Chr IV from 923802-921922, reverse complement, Verified ORF, "mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p)" [yeast-contam_custom.fasta] [chemostatdb.fasta] Protein PCF11 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PCF11 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PCF11 SGDID:S000002636, Chr IV from 923806-921926, Genome Release 64-1-1, reverse complement, Verified ORF, "mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping" [yeast-030211-contam.fasta] PCF11 SGDID:S000002636, Chr IV from 923806-921926, Genome Release 64-2-1, reverse complement, Verified ORF, "mRNA 3' end processing factor; essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping; relocalizes to the cytosol in response to hypoxia" [20141208_orf_trans.fasta] YDR228C SGDID:S000002636, Chr IV from 923806-921926, Genome Release 64-1-1, reverse complement, Verified ORF, "mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	626 amino acids
Gene Ontology:
Cellular Component:	mRNA cleavage factor complex [IPI]
Biological Process:	termination of RNA polymerase II transcription [IDA] mRNA polyadenylation [IDA] mRNA cleavage [IDA]
Molecular Function:	protein binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDHDTEVIVK DFNSILEELT FNSRPIITTL TKLAEENISC AQYFVDAIES RIEKCMPKQK  60
   61 LYAFYALDSI CKNVGSPYTI YFSRNLFNLY KRTYLLVDNT TRTKLINMFK LWLNPNDTGL 120
  121 PLFEGSALEK IEQFLIKASA LHQKNLQAML PTPTVPLLLR DIDKLTCLTS ERLKNQPNDE 180
  181 KLKMKLLVLS QLKQELKREK LTLNALKQVQ MQLRQVFSQD QQVLQERMRY HELQQQQQQQ 240
  241 QQQQQQQQQQ QQQYHETKDM VGSYTQNSNS AIPLFGNNSD TTNQQNSLSS SLFGNISGVE 300
  301 SFQEIEKKKS LNKINNLYAS LKAEGLIYTP PKESIVTLYK KLNGHSNYSL DSHEKQLMKN 360
  361 LPKIPLLNDI LSDCKAYFAT VNIDVLNNPS LQLSEQTLLQ ENPIVQNNLI HLLYRSKPNK 420
  421 CSVCGKRFGN SESEKLLQNE HLDWHFRINT RIKGSQNTAN TGISNSNLNT TTTRKNIQSR 480
  481 NWYLSDSQWA AFKDDEITST KHKNDYTDPH ANKNIDKSAL NIHADENDEG SVDNTLGSDR 540
  541 SNELEIRGKY VVVPETSQDM AFKCPICKET VTGVYDEESG EWVWKNTIEV NGKYFHSTCY 600
  601 HETSQNSSKS NSGKVGLDDL KKLVTK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CFT2 CLP1 PAP1 PCF11 RNA14 RNA15 YSH1
View Details	(MIPS) Mewes HW, et al. (2004)		CLP1 HRP1 PCF11 RNA14 RNA15
View Details	Gavin AC, et al. (2006)		CLP1 PCF11 RNA14 RNA15
View Details	Gavin AC, et al. (2002)		ACT1 AIM31 CFT1 CFT2 CKA1 CLP1 ENO2 FIP1 GLC7 HHF1, HHF2 HTA1 MPE1 PAP1 PCF11 PFK1 PFS2 PTA1 PTI1 REF2 RNA14 RNA15 RSA3 SEC13 SEC31 SSA3 SSU72 SYC1 TAF6 TIF4632 TYE7 VID24 VMR1 VPS53 YCL046W YSH1 YTH1
View Details	Qiu et al. (2008)		PCF11 RNA14 RNA15

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NAF1	No Comments	Hazbun TR, et al. (2003)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PCF11	PAT1	2	Unpublished Fields Lab Data
View Screen	PCF11	YLR177W	2	Unpublished Fields Lab Data
View Screen	PCF11	PPZ1	2	Unpublished Fields Lab Data
View Screen	PCF11	ADA2	2	Unpublished Fields Lab Data
View Screen	PCF11	SPP382	2	Unpublished Fields Lab Data
View Screen	PCF11	RNA14	2	Unpublished Fields Lab Data
View Screen	PCF11	GLN3	2	Unpublished Fields Lab Data
View Screen	PCF11	ARB1	2	Unpublished Fields Lab Data
View Screen	PCF11	NUP157	2	Unpublished Fields Lab Data
View Screen	PCF11	SAP1	2	Unpublished Fields Lab Data
View Screen	PCF11	RNA15	2	Unpublished Fields Lab Data
View Screen	PCF11	NAB3	2	Unpublished Fields Lab Data
View Screen	PCF11	ATG11	2	Unpublished Fields Lab Data
View Screen	PCF11	RNA15	2	Unpublished Fields Lab Data
View Screen	PCF11	SPP382	2	Uetz P, et al. (2000)
View Screen	PCF11	RNA14	2	Uetz P, et al. (2000)
View Screen	PCF11	ADA2	2	Uetz P, et al. (2000)
View Screen	PCF11	CLP1	2	Uetz P, et al. (2000)
View Screen	PCF11	NSL1	2	Unpublished Fields Lab Data
View Screen	PCF11	PAT1	2	Unpublished Fields Lab Data
View Screen	PCF11	FIP1	2	Unpublished Fields Lab Data
View Screen	PCF11	YLR149C	2	Unpublished Fields Lab Data
View Screen	PCF11	ATG11	2	Unpublished Fields Lab Data
View Screen	PCF11	RNA15	2	Uetz P, et al. (2000)
View Screen	PCF11	ATG11	2	Uetz P, et al. (2000)
View Screen	PCF11	RAD54	1	Unpublished Fields Lab Data
View Screen	PCF11	PTC3	1	Unpublished Fields Lab Data
View Screen	PCF11	YLR108C	1	Unpublished Fields Lab Data
View Screen	PCF11	RAV2	1	Unpublished Fields Lab Data
View Screen	PCF11	SFH1	1	Unpublished Fields Lab Data
View Screen	PCF11	MRPL51	1	Unpublished Fields Lab Data
View Screen	PCF11	COG2	1	Unpublished Fields Lab Data
View Screen	PCF11	ATG11	1	Unpublished Fields Lab Data
View Screen	PCF11	HAP2	1	Unpublished Fields Lab Data
View Screen	PCF11	NKP1	1	Unpublished Fields Lab Data
View Screen	PCF11	MLC2	1	Unpublished Fields Lab Data
View Screen	PCF11	HSV2	1	Unpublished Fields Lab Data
View Screen	PCF11	BSP1	1	Unpublished Fields Lab Data
View Screen	PCF11	SEC9	1	Unpublished Fields Lab Data
View Screen	PCF11	ADA2	1	Unpublished Fields Lab Data
View Screen	PCF11	PAT1	1	Unpublished Fields Lab Data
View Screen	PCF11	CDC23	1	Unpublished Fields Lab Data
View Screen	PCF11	PEX8	1	Unpublished Fields Lab Data
View Screen	PCF11	SPC25	1	Unpublished Fields Lab Data
View Screen	PCF11	YMR290W-A	1	Unpublished Fields Lab Data
View Screen	PCF11	YDR506C	1	Unpublished Fields Lab Data
View Screen	PCF11	YIP3	1	Unpublished Fields Lab Data
View Screen	PCF11	SLX5	1	Unpublished Fields Lab Data
View Screen	PCF11	STB5	1	Unpublished Fields Lab Data
View Screen	PCF11	ZPR1	1	Unpublished Fields Lab Data
View Screen	PCF11	WTM2	1	Unpublished Fields Lab Data
View Screen	PCF11	YPT53	1	Unpublished Fields Lab Data
View Screen	PCF11	PRP9	1	Unpublished Fields Lab Data
View Screen	PCF11	FIP1	1	Unpublished Fields Lab Data
View Screen	PCF11	YHL049C	1	Unpublished Fields Lab Data
View Screen	PCF11	CLP1	1	Unpublished Fields Lab Data
View Screen	PCF11	CNM67	1	Unpublished Fields Lab Data
View Screen	PCF11	YFH1	1	Unpublished Fields Lab Data
View Screen	PCF11	DAL80	1	Unpublished Fields Lab Data
View Screen	PCF11	FIP1	1	Unpublished Fields Lab Data
View Screen	PCF11	HAC1	1	Unpublished Fields Lab Data
View Screen	PCF11	NDD1	1	Unpublished Fields Lab Data
View Screen	PCF11	HOM3	1	Unpublished Fields Lab Data
View Screen	PCF11	NBA1	1	Unpublished Fields Lab Data
View Screen	PCF11	YDL233W	1	Unpublished Fields Lab Data
View Screen	PCF11	YLR108C	1	Unpublished Fields Lab Data
View Screen	PCF11	NAP1	1	Unpublished Fields Lab Data
View Screen	PCF11	ISC10	1	Unpublished Fields Lab Data
View Screen	PCF11	LPX1	1	Unpublished Fields Lab Data
View Screen	PCF11	RTT103	1	Unpublished Fields Lab Data
View Screen	PCF11	SFH1	1	Unpublished Fields Lab Data
View Screen	PCF11	NOP56	1	Unpublished Fields Lab Data
View Screen	PCF11	YGL118C	1	Unpublished Fields Lab Data
View Screen	PCF11	THI72	1	Unpublished Fields Lab Data
View Screen	PCF11	ADA2	1	Unpublished Fields Lab Data
View Screen	PCF11	SPP382	1	Unpublished Fields Lab Data
View Screen	PCF11	FUN12	1	Unpublished Fields Lab Data
View Screen	PCF11	MDR1	1	Unpublished Fields Lab Data
View Screen	PCF11	GEM1	1	Unpublished Fields Lab Data
View Screen	PCF11	ART5	1	Unpublished Fields Lab Data
View Screen	PCF11	WTM2	1	Unpublished Fields Lab Data
View Screen	PCF11	SPC25	1	Unpublished Fields Lab Data
View Screen	PCF11	CLP1	1	Unpublished Fields Lab Data
View Screen	PCF11	YDR132C	1	Unpublished Fields Lab Data
View Screen	PCF11	YGR182C	1	Unpublished Fields Lab Data
View Screen	PCF11	EXO84	1	Unpublished Fields Lab Data
View Screen	PCF11	RIM101	1	Unpublished Fields Lab Data
View Screen	PCF11	RPL1A, RPL1B	1	Unpublished Fields Lab Data
View Screen	PCF11	SAP1	1	Unpublished Fields Lab Data
View Screen	PCF11	HAP5	1	Unpublished Fields Lab Data
View Screen	PCF11	IMP3	1	Unpublished Fields Lab Data
View Screen	PCF11	NPL4	1	Unpublished Fields Lab Data
View Screen	PCF11	CDC23	1	Unpublished Fields Lab Data
View Screen	PCF11	NUP157	1	Unpublished Fields Lab Data
View Screen	PCF11	DBP1	1	Unpublished Fields Lab Data
View Screen	PCF11	SAP1	1	Unpublished Fields Lab Data
View Screen	PCF11	SNX3	1	Unpublished Fields Lab Data
View Screen	PCF11	CDC23	1	Unpublished Fields Lab Data
View Screen	PCF11	YKL147C	1	Unpublished Fields Lab Data
View Screen	PCF11	HFI1	1	Unpublished Fields Lab Data
View Screen	PCF11	KEG1	1	Unpublished Fields Lab Data
View Screen	PCF11	NIP100	1	Unpublished Fields Lab Data
View Screen	PCF11	NUP157	1	Unpublished Fields Lab Data
View Screen	PCF11	NIP100	1	Unpublished Fields Lab Data
View Screen	PCF11	STB5	1	Unpublished Fields Lab Data
View Screen	PCF11	SHS1	1	Unpublished Fields Lab Data
View Screen	PCF11	MEH1	1	Unpublished Fields Lab Data
View Screen	PCF11	TIP41	1	Unpublished Fields Lab Data
View Screen	PCF11	RNA15	1	Unpublished Fields Lab Data
View Screen	PCF11	NAB3	1	Unpublished Fields Lab Data
View Screen	PCF11	LSM5	1	Unpublished Fields Lab Data
View Screen	PCF11	NSL1	1	Unpublished Fields Lab Data
View Screen	PCF11	YDR132C	1	Unpublished Fields Lab Data
View Screen	PCF11	LYS5	1	Unpublished Fields Lab Data
View Screen	PCF11	NTO1	1	Unpublished Fields Lab Data
View Screen	PCF11	HXT16	1	Unpublished Fields Lab Data
View Screen	PCF11	ARG82	1	Unpublished Fields Lab Data
View Screen	PCF11	PIG1	1	Unpublished Fields Lab Data
View Screen	PCF11	YPR076W	1	Unpublished Fields Lab Data
View Screen	PCF11	YGR026W	1	Unpublished Fields Lab Data
View Screen	PCF11	MCM16	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PCF11	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..117]	MSA	5.03998	View MSA. No confident structure predictions are available.
2	View Details	[118..460]	MSA	19.057997	View MSA. No confident structure predictions are available.
3	View Details	[461..514]	deduced	N/A	No confident structure predictions are available.
4	View Details	[515..626]	MSA	1.013996	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	3.44539235794744	bayes_pls_golite062009
transcription regulator activity	2.34092044533955	bayes_pls_golite062009
protein binding	1.97365365208963	bayes_pls_golite062009
nucleic acid binding	1.74252996786746	bayes_pls_golite062009
DNA binding	1.73786982174918	bayes_pls_golite062009
transcription factor activity	1.0565013251721	bayes_pls_golite062009
clathrin binding	0.196939581490181	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2