Protein Overview: SUP35

General Information:
Name(s) found:	SUP35 / YDR172W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|EFBYS2 suppressor 2 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6320377\|ref\|NP_010457.1\| Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype [NCBI NR] gi\|4582\|emb\|CAA30155.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|172791\|gb\|AAA35133.1\| omnipotent suppressor (alt.) [NCBI NR] gi\|135056\|sp\|P05453.1\|ERF3_YEAST RecName: Full=Eukaryotic peptide chain release factor GTP-binding subunit; AltName: Full=ERF2; AltName: Full=ERF-3; Short=ERF3; AltName: Full=Translation release factor 3; AltName: Full=Polypeptide release factor 3; AltNam [NCBI NR] gi\|1289287\|emb\|CAA86677.1\| Sup2p [Saccharomyces cerevisiae] [NCBI NR] Eukaryotic peptide chain release factor GTP-binding subunit OS=Saccharomyces cerevisiae GN=SUP35 PE=1 SV=1 [Swiss-Prot] Eukaryotic peptide chain release factor GTP-binding subunit - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] SUP35 Eukaryotic peptide chain release factor GTP-binding subunit [mips] Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype [SGD] SUP35, Chr IV from 808317-810374 [yeast_orfs2] Chr IV from 808317-810374 [yeastorf3.fasta] (P05453) Eukaryotic peptide chain release factor GTP-binding subunit (ERF2) (Translation release factor 3) (ERF3) (ERF-3) (Omnipotent suppressor protein 2) (G1 to S phase transition protein 1) [4932.FASTACCERV.txt] SUP35 SGDID:S0002579, Chr IV from 808318-810375, Verified ORF [yeastflysequest.fasta] SUP35 SGDID:S0002579, Chr IV from 808319-810376, Verified ORF [10112005cervSGD.fasta] Sup35p [Saccharomyces cerevisiae]gi\|1289287\|emb\|CAA86677.1\| Sup2p [Saccharomyces cerevisiae]gi\|4582\|emb\|CAA30155.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|72877\|pir\|\|EFBYS2 suppressor 2 protein - yeast (Saccharomyces cerevisiae)gi\|172791 [nrdb_11152004_Chlamydomonas] SUP35 SGDID:S000002579, Chr IV from 808319-810376, Verified ORF, "Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and c [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SUP35 SGDID:S000002579, Chr IV from 808321-810378, Verified ORF, "Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and c [yeast-contam.fasta] SUP35 SGDID:S000002579, Chr IV from 808322-810379, Verified ORF, "Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and c [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Eukaryotic peptide chain release factor GTP-binding subunit (ERF2) (Translation release factor 3) (Polypeptide release factor 3) (ERF3) (ERF-3) (Omnipotent suppressor protein 2) (G1 to S phase transition protein 1) [nr-271106-contam.fasta] omnipotent suppressor (alt.) [nr-271106-contam.fasta] Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype; Sup35p [Saccharomyces cere [nr-271106-contam.fasta] Sup2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SUP35 SGDID:S000002579, Chr IV from 808322-810379, Verified ORF, "Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype" [yeast-bovrabprots.fasta] SUP35 SGDID:S000002579, Chr IV from 808321-810378, Verified ORF, "Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype" [yeast-contam_custom.fasta] [chemostatdb.fasta] Eukaryotic peptide chain release factor GTP-binding subunit OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SUP35 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SUP35 SGDID:S000002579, Chr IV from 808324-810381, Genome Release 64-1-1, Verified ORF, "Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype" [yeast-030211-contam.fasta] SUP35 SGDID:S000002579, Chr IV from 808324-810381, Genome Release 64-2-1, Verified ORF, "Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)]" [20141208_orf_trans.fasta] YDR172W SGDID:S000002579, Chr IV from 808324-810381, Genome Release 64-1-1, Verified ORF, "Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	685 amino acids
Gene Ontology:
Cellular Component:	cytosol [TAS] translation release factor complex [IDA]
Biological Process:	translational termination [TAS] nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IMP][IPI]
Molecular Function:	translation release factor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDSNQGNNQ QNYQQYSQNG NQQQGNNRYQ GYQAYNAQAQ PAGGYYQNYQ GYSGYQQGGY  60
   61 QQYNPDAGYQ QQYNPQGGYQ QYNPQGGYQQ QFNPQGGRGN YKNFNYNNNL QGYQAGFQPQ 120
  121 SQGMSLNDFQ KQQKQAAPKP KKTLKLVSSS GIKLANATKK VGTKPAESDK KEEEKSAETK 180
  181 EPTKEPTKVE EPVKKEEKPV QTEEKTEEKS ELPKVEDLKI SESTHNTNNA NVTSADALIK 240
  241 EQEEEVDDEV VNDMFGGKDH VSLIFMGHVD AGKSTMGGNL LYLTGSVDKR TIEKYEREAK 300
  301 DAGRQGWYLS WVMDTNKEER NDGKTIEVGK AYFETEKRRY TILDAPGHKM YVSEMIGGAS 360
  361 QADVGVLVIS ARKGEYETGF ERGGQTREHA LLAKTQGVNK MVVVVNKMDD PTVNWSKERY 420
  421 DQCVSNVSNF LRAIGYNIKT DVVFMPVSGY SGANLKDHVD PKECPWYTGP TLLEYLDTMN 480
  481 HVDRHINAPF MLPIAAKMKD LGTIVEGKIE SGHIKKGQST LLMPNKTAVE IQNIYNETEN 540
  541 EVDMAMCGEQ VKLRIKGVEE EDISPGFVLT SPKNPIKSVT KFVAQIAIVE LKSIIAAGFS 600
  601 CVMHVHTAIE EVHIVKLLHK LEKGTNRKSK KPPAFAKKGM KVIAVLETEA PVCVETYQDY 660
  661 PQLGRFTLRD QGTTIAIGKI VKIAE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		SUP35 SUP45
View Details	Krogan NJ, et al. (2006)		PLB3 RPS10B SCM3 SUP35 SUP45 TRM12 VPS3
View Details	Gavin AC, et al. (2006)		SUP35 SUP45
View Details	Gavin AC, et al. (2002)		CLU1 CRN1 ECM29 KAP123 MEC1 MIP6 MIS1 MRPL23 NAB3 NAB6 NRD1 ROM2 RPA135 SEN54 SUI3 SUP35 SUP45 TAO3 USO1 UTP20 VAC14 VPS5 YJL017W
View Details	Qiu et al. (2008)		SUP35 SUP45

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	NOP9	No Comments	Hazbun TR, et al. (2003)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	KOG1	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment	Keck JM, et al. (2011)
View Run	MLP2	#05 Alpha Factor Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#07 Alpha Factor Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#09 Alpha Factor Prep4-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PLM2	SUP35	1	Unpublished Fields Lab Data
View Screen	RAD51	SUP35	1	Unpublished Fields Lab Data
View Screen	UBI4	SUP35	1	Unpublished Fields Lab Data
View Screen	SPT21	SUP35	1	Unpublished Fields Lab Data
View Screen	CDC53	SUP35	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SUP35	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..254]	ORFEUS	13.27	No description for 1ldvA was found.
2	View Details	[255..482]	PSI-BLAST	1862.218487	Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain
3	View Details	[483..576]	PSI-BLAST	1862.218487	Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain
4	View Details	[577..685]	PSI-BLAST	1862.218487	Elongation factor eEF-1alpha, domain 2; Elongation factor eEF-1alpha, C-terminal domain; Elongation factor eEF-1alpha, N-terminal (G) domain

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
translation elongation factor activity	8.90683166637832	bayes_pls_golite062009
translation factor activity, nucleic acid binding	4.8378725567094	bayes_pls_golite062009
translation regulator activity	4.79814772079421	bayes_pls_golite062009
structural constituent of ribosome	4.56509805974548	bayes_pls_golite062009
structural molecule activity	3.58406348799895	bayes_pls_golite062009
transporter activity	3.10463051760206	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.95079405147356	bayes_pls_golite062009
pyrophosphatase activity	2.87427509025394	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.85499564954033	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.85178667290149	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
hydrolase activity	2.55157966585974	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
translation release factor activity	2.49148102494524	bayes_pls_golite062009
translation termination factor activity	2.46464632547226	bayes_pls_golite062009
primary active transmembrane transporter activity	2.39629638016861	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.39600931083427	bayes_pls_golite062009
ATPase activity	2.39535714892224	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.36051733655256	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.35906629925754	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.35679406876731	bayes_pls_golite062009
RNA binding	2.29590582680214	bayes_pls_golite062009
ATPase activity, coupled	2.24901251684901	bayes_pls_golite062009
actin filament binding	2.1683792904715	bayes_pls_golite062009
	2.15810909411803	bayes_pls_golite062009
motor activity	2.11140484670646	bayes_pls_golite062009
substrate-specific transporter activity	1.65609907941094	bayes_pls_golite062009
nucleic acid binding	1.49633029954726	bayes_pls_golite062009
actin binding	1.42666717383185	bayes_pls_golite062009
cytoskeletal protein binding	1.40488517180956	bayes_pls_golite062009
microtubule motor activity	1.35938517416356	bayes_pls_golite062009
microfilament motor activity	1.33993901826495	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.3182108176243	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
transcription regulator activity	0.989226939888218	bayes_pls_golite062009
ATP binding	0.98146619596929	bayes_pls_golite062009
DNA binding	0.967303417236485	bayes_pls_golite062009
adenyl ribonucleotide binding	0.949788783884952	bayes_pls_golite062009
adenyl nucleotide binding	0.931486373329219	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
protein methyltransferase activity	0.876296121718575	bayes_pls_golite062009
mRNA binding	0.76699944561918	bayes_pls_golite062009
ion transmembrane transporter activity	0.704934361725919	bayes_pls_golite062009
kinase activity	0.691112783105676	bayes_pls_golite062009
histone methyltransferase activity	0.63002958837536	bayes_pls_golite062009
RNA helicase activity	0.6022754485555	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.499834566483656	bayes_pls_golite062009
GTPase activity	0.437665353870877	bayes_pls_golite062009
anion transmembrane-transporting ATPase activity	0.353150780751661	bayes_pls_golite062009
guanyl nucleotide binding	0.296406132096546	bayes_pls_golite062009
GTP binding	0.295705904888768	bayes_pls_golite062009
guanyl ribonucleotide binding	0.29028316917561	bayes_pls_golite062009
cation transmembrane transporter activity	0.282397764628576	bayes_pls_golite062009
ribonuclease activity	0.222424272323909	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
inorganic anion transmembrane transporter activity	0.12168417344278	bayes_pls_golite062009
helicase activity	0.0775311459068899	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.0716139935283535	bayes_pls_golite062009
transcription factor activity	0.0434163635422169	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.0430764033739892	bayes_pls_golite062009
protein transporter activity	0.0397092337273124	bayes_pls_golite062009
protein transmembrane transporter activity	0.0378638259240902	bayes_pls_golite062009
protein kinase activity	0.0364322017534667	bayes_pls_golite062009
peptide transporter activity	0.0135927378126404	bayes_pls_golite062009
signal sequence binding	6.53725193420218E-4	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2