Protein Overview: TAF12

General Information:
Name(s) found:	TAF12 / YDR145W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|899395\|emb\|CAA90368.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6320349\|ref\|NP_010429.1\| Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A; Taf12p [Saccharomyces cerevisiae] [NCBI NR] gi\|42559733\|sp\|Q03761.1\|TAF12_YEAST RecName: Full=Transcription initiation factor TFIID subunit 12; AltName: Full=TBP-associated factor 12; AltName: Full=TBP-associated factor 61 kDa; AltName: Full=TAFII-61; Short=TAFII61; AltName: Full=TAFII-68; Short=TA [NCBI NR] pir\|\|S57972 hypothetical protein YDR145w - yeast (Saccharomyces cerevisiae) [NCBI NR] (Q03761) Transcription initiation factor TFIID subunit 12 (TBP-associated factor 12) (TBP-associated factor 61 kDa) (TAFII-61) (TAFII61) (TAFII-68) (TAFII68) [Swiss-Prot] Transcription initiation factor TFIID subunit 12 OS=Saccharomyces cerevisiae GN=TAF12 PE=1 SV=1 [Swiss-Prot] TAF12 TFIID and SAGA subunit [mips] Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A [SGD] TAF61, Chr IV from 746731-748350 [yeast_orfs2] Chr IV from 746731-748350 [yeastorf3.fasta] >Q03761 (Q03761) Hypothetical protein [4932.FASTACCERV.txt] TAF12 SGDID:S0002552, Chr IV from 746732-748351, Verified ORF [yeastflysequest.fasta] TAF12 SGDID:S0002552, Chr IV from 746733-748352, Verified ORF [10112005cervSGD.fasta] Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A [Saccharomyces cerevisiae]gi\|899395\|emb\|CAA90368.1\| unknown [Saccharomyces cerevisiae]gi\|425597 [nrdb_11152004_Chlamydomonas] TAF12 SGDID:S000002552, Chr IV from 746733-748352, Verified ORF, "Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] TAF12 SGDID:S000002552, Chr IV from 746735-748354, Verified ORF, "Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A" [yeast-contam.fasta] TAF12 SGDID:S000002552, Chr IV from 746736-748355, Verified ORF, "Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Transcription initiation factor TFIID subunit 12 (TBP-associated factor 12) (TBP-associated factor 61 kDa) (TAFII-61) (TAFII61) (TAFII-68) (TAFII68) [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A; Taf12p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Transcription initiation factor TFIID subunit 12 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TAF12 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] TAF12 SGDID:S000002552, Chr IV from 746738-748357, Genome Release 64-1-1, Verified ORF, "Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A" [yeast-030211-contam.fasta] TAF12 SGDID:S000002552, Chr IV from 746738-748357, Genome Release 64-2-1, Verified ORF, "Subunit (61/68 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A" [20141208_orf_trans.fasta] YDR145W SGDID:S000002552, Chr IV from 746738-748357, Genome Release 64-1-1, Verified ORF, "Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	539 amino acids
Gene Ontology:
Cellular Component:	transcription factor TFIID complex [IDA][TAS] SLIK (SAGA-like) complex [IPI] SAGA complex [IDA]
Biological Process:	chromatin modification [TAS] protein amino acid acetylation [IPI] histone acetylation [TAS] chromatin organization [IPI] regulation of transcription involved in G1 phase of mitotic cell cycle [IPI ] transcription initiation from RNA polymerase II promoter [TAS ]
Molecular Function:	general RNA polymerase II transcription factor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSNPENSGV NANNNTGTGN ADAITGAQQN MVLQPRQLQE MAAKFRTLLT EARNVGETTP  60
   61 RGKELMFQAA KIKQVYDALT LNRRRQQAAQ AYNNTSNSNS SNPASIPTEN VPNSSQQQQQ 120
  121 QQQQTRNNSN KFSNMIKQVL TPEENQEYEK LWQNFQVRHT SIKEKETYLK QNIDRLEQEI 180
  181 NKQTDEGPKQ QLQEKKIELL NDWKVLKIEY TKLFNNYQNS KKTFYVECAR HNPALHKFLQ 240
  241 ESTQQQRVQQ QRVQQQQQQQ QQQQQQQQQQ QQQQQQRQGQ NQRKISSSNS TEIPSVTGPD 300
  301 ALKSQQQQQN TITATNNPRG NVNTSQTEQS KAKVTNVNAT ASMLNNISSS KSAIFKQTEP 360
  361 AIPISENIST KTPAPVAYRS NRPTITGGSA MNASALNTPA TTKLPPYEMD TQRVMSKRKL 420
  421 RELVKTVGID EGDGETVIDG DVEELLLDLA DDFVTNVTAF SCRLAKHRKS DNLEARDIQL 480
  481 HLERNWNIRI PGYSADEIRS TRKWNPSQNY NQKLQSITSD KVAAAKNNGN NVASLNTKK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ADA2 GCN5 HFI1 NGG1 SGF11 SGF29 SGF73 SPT15 SPT20 SPT3 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF3 TAF4 TAF5 TAF6 TAF7 TAF9
View Details	(MIPS) Mewes HW, et al. (2004)		ADA2 GCN5 HFI1 NGG1 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF5 TAF6 TAF7 TAF9 TRA1
View Details	Krogan NJ, et al. (2006)		ADA2 AHC1 AHC2 FMP40 GCN5 HFI1 MSS4 NGG1 PDR15 RAD59 REV3 SGF11 SGF29 SGF73 SNL1 SPT20 SPT3 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF3 TAF4 TAF5 TAF6 TAF7 TAF9 UBP8 YMR007W
View Details	Gavin AC, et al. (2006)		SPT15 TAF1 TAF11 TAF12 TAF3 TAF4 TAF9
View Details	Gavin AC, et al. (2002)		ADA2 ADH1 CLU1 FUN19 GCN5 HEM13 HFI1 KAP114 KAP123 MED4 MES1 MNN4 MOT1 MYO1 MYO4 NGG1 NOP1 PFK2 PSK1 PTC1 PUF6 RPB2 RPN9 RPO21 RPT5 RVB2 SGF29 SGF73 SIN3 SIN4 SPT15 SPT20 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TBF1 TEF4 UBP8 VAS1 VPS1
View Details	Qiu et al. (2008)		ADA2 AHC1 CHD1 EPL1 GAL11 GCN5 HFI1 MED1 MED2 NGG1 NUT1 NUT2 PGD1 RGR1 ROX3 SGF11 SGF29 SGF73 SIN4 SPT20 SPT3 SPT7 SPT8 SRB4 SRB6 SSL2 SUS1 SWC4 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TFA1 TFB5 TFG1 TFG2 TRA1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YHR122W	TAF12	1	Hazbun TR, et al. (2003)
View Screen	SDO1	TAF12	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TAF12	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..232]	deduced	N/A	No confident structure predictions are available.
2	View Details	[233..405]	MSA	4.013997	View MSA. No confident structure predictions are available.
3	View Details	[406..539]	ORFEUS	14.64	TAF(II)-20, (TAF(II)-15, hTAF12), histone fold domain

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
general RNA polymerase II transcription factor activity	9.25279783093877	bayes_pls_golite062009
histone acetyltransferase activity	5.97252613675094	bayes_pls_golite062009
lysine N-acetyltransferase activity	5.97252613675094	bayes_pls_golite062009
N-acetyltransferase activity	4.49886535711761	bayes_pls_golite062009
DNA binding	4.49118409263799	bayes_pls_golite062009
nucleic acid binding	4.35201412176948	bayes_pls_golite062009
N-acyltransferase activity	4.22222466548509	bayes_pls_golite062009
transcription regulator activity	4.08606081850613	bayes_pls_golite062009
transcription initiation factor activity	3.96621818094279	bayes_pls_golite062009
acetyltransferase activity	3.91614625961398	bayes_pls_golite062009
acyltransferase activity	3.38611688546892	bayes_pls_golite062009
transferase activity, transferring acyl groups other than amino-acyl groups	3.35015681315177	bayes_pls_golite062009
RNA polymerase II transcription factor activity	3.28941584139552	bayes_pls_golite062009
binding	3.27510691458843	bayes_pls_golite062009
transferase activity, transferring acyl groups	3.1782337747057	bayes_pls_golite062009
transcription factor activity	2.51581622888695	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.82448881874976	bayes_pls_golite062009
transcription activator activity	1.25487495162413	bayes_pls_golite062009
transcription factor binding	1.15204005455906	bayes_pls_golite062009
transcription coactivator activity	1.1297723528047	bayes_pls_golite062009
nucleotidyltransferase activity	1.10331930589736	bayes_pls_golite062009
protein binding	1.07873333188144	bayes_pls_golite062009
transcription cofactor activity	1.01502031330902	bayes_pls_golite062009
transcription repressor activity	0.72554769377984	bayes_pls_golite062009
histone binding	0.218942897869955	bayes_pls_golite062009
sequence-specific DNA binding	0.0852726470007639	bayes_pls_golite062009
transcription corepressor activity	0.0736756545988504	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2