Abstract
A number of transcriptional coactivator proteins have been identified as histone acetyltransferase (HAT) proteins, providing a direct molecular basis for the coupling of histone acetylation and transcriptional activation. The yeast Spt-Ada-Gcn5-acetyltransferase (SAGA) complex requires the coactivator protein Gcn5 for HAT activity. Identification of protein subunits by mass spectrometry and immunoblotting revealed that the TATA binding protein-associated factors (TAF(II)s) TAF(II)90, -68/61, -60, -25/23, and -20/17 are integral components of this complex. In addition, TAF(II)68 was required for both SAGA-dependent nucleosomal HAT activity and transcriptional activation from chromatin templates in vitro. These results illustrate a role for certain TAF(II) proteins in the regulation of gene expression at the level of chromatin modification that is distinct from the TFIID complex and TAF(II)145.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetyltransferases / metabolism*
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / metabolism*
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Histone Acetyltransferases
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Histones / metabolism
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Nucleosomes / metabolism*
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Protein Kinases / metabolism
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Saccharomyces cerevisiae
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Saccharomyces cerevisiae Proteins*
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TATA-Binding Protein Associated Factors*
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TATA-Box Binding Protein
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Transcription Factor TFIID
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Transcription Factors / metabolism*
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Transcription, Genetic*
Substances
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DNA-Binding Proteins
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Fungal Proteins
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Histones
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NGG1 protein, S cerevisiae
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Nucleosomes
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SPT3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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TAF12 protein, S cerevisiae
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TATA-Binding Protein Associated Factors
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TATA-Box Binding Protein
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Transcription Factor TFIID
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Transcription Factors
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Acetyltransferases
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GCN5 protein, S cerevisiae
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Histone Acetyltransferases
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Protein Kinases