Protein Overview: PRP11

General Information:
Name(s) found:	PRP11 / YDL043C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320161\|ref\|NP_010241.1\| Subunit of the SF3a splicing factor complex, required for spliceosome assembly; Prp11p [Saccharomyces cerevisiae] [NCBI NR] gi\|2498800\|sp\|Q07350.1\|PRP11_YEAST RecName: Full=Pre-mRNA-splicing factor PRP11 [NCBI NR] gi\|226088\|prf\|\|1410273A RNA11 gene [NCBI NR] pir\|\|S67576 PRP11 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1431030\|emb\|CAA98602.1\| PRP11 [Saccharomyces cerevisiae] [NCBI NR] Pre-mRNA-splicing factor PRP11 OS=Saccharomyces cerevisiae GN=PRP11 PE=1 SV=1 [Swiss-Prot] (Q07350) Pre-mRNA splicing factor PRP11 [Swiss-Prot] PRP11 Pre-mRNA splicing factor [mips] Subunit of the SF3a splicing factor complex, required for spliceosome assembly [SGD] PRP11, Chr IV from 375676-376476, reverse complement [yeast_orfs2] Chr IV from 375676-376476, reverse complement [yeastorf3.fasta] Chr IV from 376476-375676, reverse complement [yeastorf5.fasta] PRP11 SGDID:S0002201, Chr IV from 376477-375677, reverse complement, Verified ORF [yeastflysequest.fasta] Prp11p [Saccharomyces cerevisiae]gi\|1431030\|emb\|CAA98602.1\| PRP11 [Saccharomyces cerevisiae]gi\|2133073\|pir\|\|S67576 PRP11 protein - yeast (Saccharomyces cerevisiae)gi\|2498800\|sp\|Q07350\|PR11_YEAST Pre-mRNA splicing factor PRP11gi\|226088\|prf\|\|1410273A RN [nrdb_11152004_Chlamydomonas] PRP11 SGDID:S000002201, Chr IV from 376477-375677, reverse complement, Verified ORF, "Subunit of the SF3a splicing factor complex, required for spliceosome assembly" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PRP11 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Pre-mRNA-splicing factor PRP11 [nr-271106-contam.fasta] RNA11 gene [nr-271106-contam.fasta] Subunit of the SF3a splicing factor complex, required for spliceosome assembly; Prp11p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Pre-mRNA-splicing factor PRP11 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PRP11 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PRP11 SGDID:S000002201, Chr IV from 376480-375680, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the SF3a splicing factor complex, required for spliceosome assembly" [yeast-030211-contam.fasta] PRP11 SGDID:S000002201, Chr IV from 376480-375680, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of the SF3a splicing factor complex; required for spliceosome assembly" [20141208_orf_trans.fasta] YDL043C SGDID:S000002201, Chr IV from 376480-375680, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the SF3a splicing factor complex, required for spliceosome assembly" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	266 amino acids
Gene Ontology:
Cellular Component:	U2 snRNP [IDA ]
Biological Process:	spliceosome assembly [IDA]
Molecular Function:	RNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNYLEGVGSK KGGGGIASES QFNLQRRKEV ESLLSKGENV PYTFQDEKDD QVRSNPYIYK  60
   61 NHSGKLVCKL CNTMHMSWSS VERHLGGKKH GLNVLRRGIS IEKSSLGREG QTTHDFRQQQ 120
  121 KIIEAKQSLK NNGTIPVCKI ATVKNPKNGS VGLAIQVNYS SEVKENSVDS DDKAKVPPLI 180
  181 RIVSGLELSD TKQKGKKFLV IAYEPFENIA IELPPNEILF SENNDMDNNN DGVDELNKKC 240
  241 TFWDAISKLY YVQFFFKQAE QEQADV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BRR2 CLF1 CUS1 DIB1 HSH155 HSH49 LEA1 LUC7 MUD1 PRP11 PRP21 PRP3 PRP31 PRP4 PRP46 PRP6 PRP8 PRP9 RSE1 SMB1 SMD1 SMD2 SMD3 SNP1 SNU114 SNU23 SNU66 STO1 YHC1
View Details	(MIPS) Mewes HW, et al. (2004)		PRP11 PRP9
View Details	Krogan NJ, et al. (2006)		BUD13 BUD31 CUS1 CWC21 HSH155 HSH49 LEA1 LOT6 PRP11 PRP21 PRP45 PRP9 RSE1
View Details	Gavin AC, et al. (2006)		CUS1 HSH155 HSH49 LEA1 MSL1 PRP11 PRP21 PRP9 RSE1
View Details	Gavin AC, et al. (2002)		AAR2 BRR1 BUD31 CBC2 CDC33 CEF1 CLF1 CRM1 CSN12 CUS1 CWC2 CWC22 CWC23 DHH1 DIB1 ECM2 GCN2 HRT1 HSH155 HSH49 HSP42 HTA1 IMG1 KAP123 LEA1 LIN1 LSM1 LSM2 LSM4 LSM5 LSM6 LSM7 LUC7 MIR1 MRP7 MRPL28 MRPL3 MRPL35 MRPL38 MRPL4 MRPL8 MRPS5 MSL1 MUD1 NAM8 NPL3 NSL1 PAT1 PDC1 PRP11 PRP18 PRP19 PRP21 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP46 PRP6 PRP8 PRP9 RSE1 RVB2 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP381 SPP382 SRB2 STO1 SYF1 TAT1 TIF4631 TIF4632 TOS4 UTP21 YEF3 YHR020W YML6
View Details	Ho Y, et al. (2002)		ADK1 CLU1 COP1 GPH1 NAN1 PRP11 REX2 SEC27 SHM2 SSK2 TFP1 THI22 TIF4631 UBP15 UTP13 YGR250C
View Details	Qiu et al. (2008)		AAR2 BRR1 BRR2 CBC2 CDC40 CEF1 CLF1 CUS1 CUS2 CWC15 CWC2 CWC22 CWC23 DHR2 DIB1 HSH155 HSH49 IST3 ISY1 LEA1 LSM4 LSM5 LUC7 MAK5 MSL1 MUD1 MUD2 NAM8 NTC20 PRP11 PRP16 PRP18 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP31 PRP38 PRP39 PRP4 PRP40 PRP43 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNU114 SNU23 SNU56 SNU66 SNU71 SPP2 SPP381 SPP382 STO1 SUB2 SYF2 YHC1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YPR097W	PRP11	2	Unpublished Fields Lab Data
View Screen	PRP11	PRP21	2	Uetz P, et al. (2000)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PRP11	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..121]	deduced	N/A	No confident structure predictions are available.
2	View Details	[122..266]	MSA	1.008974	View MSA. No confident structure predictions are available.
3	View Details	[225..266]	MSA	2.032993	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
transcription regulator activity	3.37529385176635	bayes_pls_golite062009
DNA binding	3.3049326844117	bayes_pls_golite062009
nucleic acid binding	3.24981360535232	bayes_pls_golite062009
snRNA binding	3.16782731106334	bayes_pls_golite062009
RNA binding	3.11293528368816	bayes_pls_golite062009
U1 snRNA binding	2.86343975521155	bayes_pls_golite062009
binding	2.84954567129719	bayes_pls_golite062009
transcription factor activity	2.44389510173662	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.57041104288348	bayes_pls_golite062009
mRNA binding	1.28525296322454	bayes_pls_golite062009
transcription activator activity	1.20323819132961	bayes_pls_golite062009
protein binding	0.975412203442928	bayes_pls_golite062009
sequence-specific DNA binding	0.732558534458316	bayes_pls_golite062009
transcription repressor activity	0.686318575498149	bayes_pls_golite062009
chromatin binding	0.626365497850853	bayes_pls_golite062009
transcription factor binding	0.515821187939278	bayes_pls_golite062009
catalytic activity	0.263007611601737	bayes_pls_golite062009
specific RNA polymerase II transcription factor activity	0.200483461579207	bayes_pls_golite062009
transcription cofactor activity	0.113925660147477	bayes_pls_golite062009
structure-specific DNA binding	0.0855506955761403	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2