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Protein Overview: SPT15

General Information:
Name(s) found: SPT15 / YER148W [SGD]
Description(s) found:
Found 35 descriptions.
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  • TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 240 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    RNA polymerase I transcription factor complex [TASLink to PubMed article]
    transcription factor TFIID complex [IDALink to PubMed article][TASLink to PubMed article]
    transcription factor TFIIIB complex [IDALink to PubMed article][TASLink to PubMed article]
    Biological Process: transcription from RNA polymerase III promoter [IMPLink to PubMed article]
    transcription initiation from RNA polymerase III promoter [TASLink to PubMed article]
    transcription from RNA polymerase I promoter [IMPLink to PubMed article][TASLink to PubMed article]
    transcription from RNA polymerase II promoter [IMPLink to PubMed article]
    transcription initiation from RNA polymerase II promoter [TASLink to PubMed articleLink to PubMed articleLink to PubMed article]
    Molecular Function: DNA binding [IPILink to PubMed article][TASLink to PubMed article]
    RNA polymerase I transcription factor activity [IMPLink to PubMed article][TASLink to PubMed article]
    protein homodimerization activity [IMPLink to PubMed article]
    general RNA polymerase II transcription factor activity [IMPLink to PubMed article][TASLink to PubMed article]
    DNA bending activity [IDALink to PubMed article]
    RNA polymerase III transcription factor activity [IMPLink to PubMed article][TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MADEERLKEF KEANKIVFDP NTRQVWENQN RDGTKPATTF QSEEDIKRAA PESEKDTSAT  60
   61 SGIVPTLQNI VATVTLGCRL DLKTVALHAR NAEYNPKRFA AVIMRIREPK TTALIFASGK 120
  121 MVVTGAKSED DSKLASRKYA RIIQKIGFAA KFTDFKIQNI VGSCDVKFPI RLEGLAFSHG 180
  181 TFSSYEPELF PGLIYRMVKP KIVLLIFVSG KIVLTGAKQR EEIYQAFEAI YPVLSEFRKM 240
  241

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) MOT1 SPT15 TAF1 TAF11 TAF12 TAF3 TAF4 TAF5 TAF6 TAF9 VPS1
    View Details (MIPS) Mewes HW, et al. (2004) BDP1 BRF1 RRN11 RRN6 RRN7 SPT15
    View Details Krogan NJ, et al. (2006) BRF1 BRL1 SPT15
    View Details Gavin AC, et al. (2006) SPT15 TAF1 TAF11 TAF12 TAF3 TAF4 TAF9
    View Details Gavin AC, et al. (2002) ADA2 ADH1 CLU1 ESC8 FUN19 GCN1 GCN5 HEM13 IOC3 ISW1 ITC1 KAP114 KAP123 MED4 MES1 MNN4 MOT1 MYO1 NGG1 NOP1 NPL6 PDC1 PFK2 PSK1 PTC1 PUF6 RPB2 RPC40 RPN9 RPO21 RPT5 RSC2 RSC4 RSC58 RSC6 RSC8 RVB2 SGF29 SGF73 SIN3 SIN4 SPT15 SPT20 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TBF1 TEF4 VAS1 VPS1 YEF3
    View Details Qiu et al. (2008) BDP1 BRF1 PZF1 ROX3 RRN11 RRN3 RRN5 RRN6 RRN7 SPT15 TAF1 TAF10 TAF11 TAF13 TAF14 TAF3 TAF5 TAF6 TAF9 TFB5 TFC4 TFC8 UAF30

    Mass Spectrometry Data

    No mass spectrometry results found for this protein.

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen IST3 SPT15 2 Uetz P, et al. (2000)

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data SPT15 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..69]
    [159..240]    		 PSI-BLAST 1037.616428 TATA-box binding protein (TBP), C-terminal domain
    2 View Details [70..158] PSI-BLAST 1037.616428 TATA-box binding protein (TBP), C-terminal domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • general RNA polymerase II transcription factor activity
    		•  10.1927670598446 bayes_pls_golite062009
  • RNA polymerase III transcription factor activity
    		•  8.4318102330583 bayes_pls_golite062009
  • transcription regulator activity
    		•  4.66773099672992 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  3.97397050058114 bayes_pls_golite062009
  • DNA binding
    		•  3.68758425895596 bayes_pls_golite062009
  • nucleic acid binding
    		•  3.4119217152884 bayes_pls_golite062009
  • binding
    		•  3.25203283623613 bayes_pls_golite062009
  • RNA polymerase I transcription factor activity
    		•  3.24777019705869 bayes_pls_golite062009
  • transcription factor activity
    		•  2.17821704710246 bayes_pls_golite062009
  • transcription factor binding
    		•  1.86204668268867 bayes_pls_golite062009
  • specific RNA polymerase II transcription factor activity
    		•  1.59579825271822 bayes_pls_golite062009
  • protein binding
    		•  0.938536348073543 bayes_pls_golite062009
  • transcription coactivator activity
    		•  0.312991469396539 bayes_pls_golite062009
  • transcription activator activity
    		•  0.199341831249925 bayes_pls_golite062009
    2 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.97

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle