Protein Overview: DNM1

General Information:
Name(s) found:	DNM1 / YLL001W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323028\|ref\|NP_013100.1\| Dynamin-related GTPase required for mitochondrial fission and the maintenance of mitochondrial morphology, assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in e [NCBI NR] pir\|\|S64742 dynamin-related protein DNM1 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1706485\|sp\|P54861.1\|DNM1_YEAST RecName: Full=Dynamin-related protein DNM1 [NCBI NR] gi\|1495224\|emb\|CAA62769.1\| L1381/DNM1 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1360157\|emb\|CAA97444.1\| DNM1 [Saccharomyces cerevisiae] [NCBI NR] Dynamin-related protein DNM1 OS=Saccharomyces cerevisiae GN=DNM1 PE=1 SV=1 [Swiss-Prot] DNM1 Dynamin-related protein [mips] Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance [SGD] DNM1, Chr XII from 147889-150162 [yeast_orfs2] Chr XII from 147889-150162 [yeastorf3.fasta] (P54861) Dynamin-related protein DNM1 (EC 3.6.5.5) [4932.FASTACCERV.txt] DNM1 SGDID:S0003924, Chr XII from 147889-150162, Verified ORF [yeastflysequest.fasta] Dnm1p [Saccharomyces cerevisiae]gi\|1360157\|emb\|CAA97444.1\| DNM1 [Saccharomyces cerevisiae]gi\|1495224\|emb\|CAA62769.1\| L1381/DNM1 protein [Saccharomyces cerevisiae]gi\|2131244\|pir\|\|S64742 dynamin-related protein DNM1 - yeast (Saccharomyces cerevisiae)gi\| [nrdb_11152004_Chlamydomonas] DNM1 SGDID:S000003924, Chr XII from 147889-150162, Verified ORF, "Dynamin-related GTPase required for mitochondrial fission and the maintenance of mitochondrial morphology, assembles on the cytoplasmic face of mitochondrial tubules at sites at which divis [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] DNM1 SGDID:S000003924, Chr XII from 147889-150162, Verified ORF, "Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates [SGD_S-cerevisiae_na_05-08-2009_con_reversed.fasta] DNM1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Dynamin-related GTPase required for mitochondrial fission and the maintenance of mitochondrial morphology, assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis; Dnm1p [Saccharom [nr-271106-contam.fasta] L1381/DNM1 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Dynamin-related protein DNM1 [nr-271106-contam.fasta] DNM1 SGDID:S000003924, Chr XII from 147889-150162, Verified ORF, "Dynamin-related GTPase required for mitochondrial fission and the maintenance of mitochondrial morphology, assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis" [yeast-bovrabprots.fasta] DNM1 SGDID:S000003924, Chr XII from 147889-150162, Verified ORF, "Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance" [scerevisihumanrev.fasta] [chemostatdb.fasta] Dynamin-related protein DNM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DNM1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DNM1 SGDID:S000003924, Chr XII from 147890-150163, Genome Release 64-1-1, Verified ORF, "Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance" [yeast-030211-contam.fasta] DNM1 SGDID:S000003924, Chr XII from 147890-150163, Genome Release 64-2-1, Verified ORF, "Dynamin-related GTPase involved in mitochondrial organization; required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance" [20141208_orf_trans.fasta] TR5907\|c0_g1_i1\|g.4106 ORF TR5907\|c0_g1_i1\|g.4106 TR5907\|c0_g1_i1\|m.4106 type:complete len:758 (+) TR5907\|c0_g1_i1:87-2360(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR3651\|c0_g1_i1\|g.2508 ORF TR3651\|c0_g1_i1\|g.2508 TR3651\|c0_g1_i1\|m.2508 type:complete len:758 (+) TR3651\|c0_g1_i1:70-2343(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YLL001W SGDID:S000003924, Chr XII from 147890-150163, Genome Release 64-1-1, Verified ORF, "Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_8371\|g.6139 ORF asmbl_8371\|g.6139 asmbl_8371\|m.6139 type:complete len:758 (+) asmbl_8371:68-2341(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_8437\|g.5294 ORF ID=asmbl_8437\|g.5294 ID=asmbl_8437\|m.5294 type:complete len:758 (+) ID=asmbl_8437:68-2341(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_8371\|g.5144 ORF ID=asmbl_8371\|g.5144 ID=asmbl_8371\|m.5144 type:complete len:758 (+) ID=asmbl_8371:68-2341(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	757 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA] mitochondrial outer membrane [IDA]
Biological Process:	mitochondrion inheritance [IGI] protein homooligomerization [IDA] mitochondrion organization [IMP] mitochondrial fission [IGI][IMP]
Molecular Function:	identical protein binding [IDA] protein homodimerization activity [IDA] GTPase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASLEDLIPT VNKLQDVMYD SGIDTLDLPI LAVVGSQSSG KSSILETLVG RDFLPRGTGI  60
   61 VTRRPLVLQL NNISPNSPLI EEDDNSVNPH DEVTKISGFE AGTKPLEYRG KERNHADEWG 120
  121 EFLHIPGKRF YDFDDIKREI ENETARIAGK DKGISKIPIN LKVFSPHVLN LTLVDLPGIT 180
  181 KVPIGEQPPD IEKQIKNLIL DYIATPNCLI LAVSPANVDL VNSESLKLAR EVDPQGKRTI 240
  241 GVITKLDLMD SGTNALDILS GKMYPLKLGF VGVVNRSQQD IQLNKTVEES LDKEEDYFRK 300
  301 HPVYRTISTK CGTRYLAKLL NQTLLSHIRD KLPDIKTKLN TLISQTEQEL ARYGGVGATT 360
  361 NESRASLVLQ LMNKFSTNFI SSIDGTSSDI NTKELCGGAR IYYIYNNVFG NSLKSIDPTS 420
  421 NLSVLDVRTA IRNSTGPRPT LFVPELAFDL LVKPQIKLLL EPSQRCVELV YEELMKICHK 480
  481 CGSAELARYP KLKSMLIEVI SELLRERLQP TRSYVESLID IHRAYINTNH PNFLSATEAM 540
  541 DDIMKTRRKR NQELLKSKLS QQENGQTNGI NGTSSISSNI DQDSAKNSDY DDDGIDAESK 600
  601 QTKDKFLNYF FGKDKKGQPV FDASDKKRSI AGDGNIEDFR NLQISDFSLG DIDDLENAEP 660
  661 PLTEREELEC ELIKRLIVSY FDIIREMIED QVPKAVMCLL VNYCKDSVQN RLVTKLYKET 720
  721 LFEELLVEDQ TLAQDRELCV KSLGVYKKAA TLISNIL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		AHA1 BUD3 CNS1 CPR6 DIG2 DNM1 DSF1, YNR073C FRT2 GPA2 HOM6 HSC82 HSP104 HSP82 MBB1 MRPL50 PAM1 PPT1 QNS1 RMD8 RTS1 SMT3 STI1 STP4 TDH1 UTP20 YBL104C
View Details	Ho Y, et al. (2002)		ABP1 ACO1 AIM41 ATP4 BUD14 CAR2 COR1 CRZ1 CYS4 DCP1 DCP2 DCS1 DNM1 EDC3 EDE1 EGD2 ENP1 GAS1 GCN3 GPH1 HHF1, HHF2 HRR25 HSP104 HXT7 HYP2 IPP1 LOC1 LSP1 LTV1 MDH1 MDS3 MGE1 NSA1 NUG1 OYE2 PEX19 PIL1 PIN4 PTC4 PUF3 RIO2 RPC19 RPM2 SAP185 SAP190 SAS10 SEC2 SEC23 SES1 SFB3 SGM1 SIT4 TMA19 TSR1 UBP15 VMA4 VPS1 YAK1 YBR225W YER138C YPL247C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	LOS1	DNM1	2	Unpublished Fields Lab Data
View Screen	NUP1	DNM1	2	Unpublished Fields Lab Data
View Screen	MSN5	DNM1	1	Unpublished Fields Lab Data
View Screen	ILM1	DNM1	1	Lockshon D, et al. (2006)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	DNM1	Huh WK, et al. (2003)
View Data	DNM1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..353]	PSI-BLAST	1175.0	Dynamin G domain
2	View Details	[354..543]	Pfam	50.318759	Dynamin central region No confident structure predictions are available.
3	View Details	[544..660]	PSI-BLAST	15.39794	Dynamin
4	View Details	[661..757]	Pfam	42.552842	Dynamin GTPase effector domain Confident ab initio structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
microtubule motor activity	6.13591371239337	bayes_pls_golite062009
motor activity	4.04927796049806	bayes_pls_golite062009
transporter activity	3.10463051760206	bayes_pls_golite062009
transmembrane transporter activity	2.95886887664984	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.95079405147356	bayes_pls_golite062009
pyrophosphatase activity	2.87427509025394	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.85499564954033	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.85178667290149	bayes_pls_golite062009
tubulin binding	2.67743463093593	bayes_pls_golite062009
plus-end-directed microtubule motor activity	2.65631438185675	bayes_pls_golite062009
microtubule binding	2.59155206524122	bayes_pls_golite062009
microfilament motor activity	2.51786963027655	bayes_pls_golite062009
active transmembrane transporter activity	2.50494515048649	bayes_pls_golite062009
cytoskeletal protein binding	2.4101693596493	bayes_pls_golite062009
primary active transmembrane transporter activity	2.39629638016861	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	2.39600931083427	bayes_pls_golite062009
ATPase activity	2.39535714892224	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	2.36051733655256	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	2.35906629925754	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	2.35679406876731	bayes_pls_golite062009
actin binding	2.2973522188675	bayes_pls_golite062009
ATPase activity, coupled	2.24901251684901	bayes_pls_golite062009
binding	2.21604035604783	bayes_pls_golite062009
substrate-specific transporter activity	1.65609907941094	bayes_pls_golite062009
hydrolase activity	1.57549510726226	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.3182108176243	bayes_pls_golite062009
actin filament binding	1.28204719195916	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
actin-dependent ATPase activity	0.92105578610354	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
GTPase activity	0.83849854735032	bayes_pls_golite062009
ion transmembrane transporter activity	0.704934361725919	bayes_pls_golite062009
molecular transducer activity	0.501012663775049	bayes_pls_golite062009
signal transducer activity	0.501012663775049	bayes_pls_golite062009
protein transporter activity	0.498432226280822	bayes_pls_golite062009
protein transmembrane transporter activity	0.46552249844591	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
signal recognition particle binding	0.29390189699022	bayes_pls_golite062009
cation transmembrane transporter activity	0.282397764628576	bayes_pls_golite062009
glutamate receptor binding	0.24961792304497	bayes_pls_golite062009
nucleic acid binding	0.194779852865789	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
minus-end-directed microtubule motor activity	0.068779631341469	bayes_pls_golite062009
structural constituent of cytoskeleton	0.0111338193708552	bayes_pls_golite062009
macromolecule transmembrane transporter activity	0.00571427458217011	bayes_pls_golite062009
P-P-bond-hydrolysis-driven protein transmembrane transporter activity	0.00571427458217011	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
actin binding	3.72754591832642	bayes_pls_golite062009
cytoskeletal protein binding	3.48744511411671	bayes_pls_golite062009
binding	2.86147407357315	bayes_pls_golite062009
protein binding	2.12168271674167	bayes_pls_golite062009
actin filament binding	2.03444575838575	bayes_pls_golite062009
signal transducer activity	1.37089850538776	bayes_pls_golite062009
molecular transducer activity	1.37089850538776	bayes_pls_golite062009
glutamate receptor binding	1.33361798466456	bayes_pls_golite062009
transporter activity	1.28986800087234	bayes_pls_golite062009
phospholipid binding	1.09683218092034	bayes_pls_golite062009
protein domain specific binding	0.90578029954417	bayes_pls_golite062009
phosphoinositide binding	0.71817045430443	bayes_pls_golite062009
substrate-specific transporter activity	0.54401901869178	bayes_pls_golite062009
receptor binding	0.50127063593663	bayes_pls_golite062009
structural constituent of cytoskeleton	0.35466574267734	bayes_pls_golite062009
phosphoinositide 3-kinase binding	0.35270637154617	bayes_pls_golite062009
receptor activity	0.249700281733733	bayes_pls_golite062009
profilin binding	0.21255426011209	bayes_pls_golite062009
clathrin binding	0.200189120318518	bayes_pls_golite062009
alpha-catenin binding	0.102004310679969	bayes_pls_golite062009
transmembrane receptor activity	0.0950861172257715	bayes_pls_golite062009
pyrophosphatase activity	0.0611639610381174	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.0524559849408587	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.0498422520006935	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.0453434814121247	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2