Protein Overview: KRS1

General Information:
Name(s) found:	KRS1 / YDR037W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|SYBYKT lysine-tRNA ligase (EC 6.1.1.6) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6320242\|ref\|NP_010322.1\| Lysyl-tRNA synthetase; also identified as a negative regulator of general control of amino acid biosynthesis; Krs1p [Saccharomyces cerevisiae] [NCBI NR] gi\|171799\|gb\|AAA66916.1\| lysyl-tRNA synthetase [NCBI NR] gi\|1431484\|emb\|CAA98863.1\| KRS1 [Saccharomyces cerevisiae] [NCBI NR] gi\|135139\|sp\|P15180.2\|SYKC_YEAST RecName: Full=Lysyl-tRNA synthetase, cytoplasmic; AltName: Full=Lysine--tRNA ligase; Short=LysRS [NCBI NR] gi\|1122359\|emb\|CAA92376.1\| Krs1p [Saccharomyces cerevisiae] [NCBI NR] Lysyl-tRNA synthetase, cytoplasmic OS=Saccharomyces cerevisiae GN=KRS1 PE=1 SV=2 [Swiss-Prot] KRS1 Lysyl-tRNA synthetase, cytosolic [mips] Lysyl-tRNA synthetase [SGD] KRS1, Chr IV from 525434-527209 [yeast_orfs2] Chr IV from 525434-527209 [yeastorf3.fasta] KRS1 SGDID:S0002444, Chr IV from 525435-527210, Verified ORF [yeastflysequest.fasta] KRS1 SGDID:S0002444, Chr IV from 525436-527211, Verified ORF [10112005cervSGD.fasta] Krs1p [Saccharomyces cerevisiae]gi\|1431484\|emb\|CAA98863.1\| KRS1 [Saccharomyces cerevisiae]gi\|1122359\|emb\|CAA92376.1\| Krs1p [Saccharomyces cerevisiae]gi\|68528\|pir\|\|SYBYKT lysine-tRNA ligase (EC 6.1.1.6) - yeast (Saccharomyces cerevisiae)gi\|135139\|sp\|P1 [nrdb_11152004_Chlamydomonas] KRS1 SGDID:S000002444, Chr IV from 525436-527211, Verified ORF, "Lysyl-tRNA synthetase; also identified as a negative regulator of general control of amino acid biosynthesis " [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] KRS1 SGDID:S000002444, Chr IV from 525437-527212, Verified ORF, "Lysyl-tRNA synthetase; also identified as a negative regulator of general control of amino acid biosynthesis " [yeast-contam.fasta] KRS1 SGDID:S000002444, Chr IV from 525438-527213, Verified ORF, "Lysyl-tRNA synthetase; also identified as a negative regulator of general control of amino acid biosynthesis" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] KRS1 SGDID:S000002444, Chr IV from 525438-527213, Verified ORF, "Lysyl-tRNA synthetase" [yeast-200209-contam.fasta] KRS1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Lysyl-tRNA synthetase, cytoplasmic (Lysine--tRNA ligase) (LysRS) [nr-271106-contam.fasta] Krs1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] lysyl-tRNA synthetase [nr-271106-contam.fasta] Lysyl-tRNA synthetase; also identified as a negative regulator of general control of amino acid biosynthesis; Krs1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Lysine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=KRS1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] KRS1 SGDID:S000002444, Chr IV from 525440-527215, Genome Release 64-1-1, Verified ORF, "Lysyl-tRNA synthetase" [yeast-030211-contam.fasta] KRS1 SGDID:S000002444, Chr IV from 525440-527215, Genome Release 64-2-1, Verified ORF, "Lysyl-tRNA synthetase" [20141208_orf_trans.fasta] YDR037W SGDID:S000002444, Chr IV from 525440-527215, Genome Release 64-1-1, Verified ORF, "Lysyl-tRNA synthetase" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	591 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [TAS]
Biological Process:	lysyl-tRNA aminoacylation [IDA]
Molecular Function:	lysine-tRNA ligase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSQQDNVKAA AEGVANLHLD EATGEMVSKS ELKKRIKQRQ VEAKKAAKKA AAQPKPASKK  60
   61 KTDLFADLDP SQYFETRSRQ IQELRKTHEP NPYPHKFHVS ISNPEFLAKY AHLKKGETLP 120
  121 EEKVSIAGRI HAKRESGSKL KFYVLHGDGV EVQLMSQLQD YCDPDSYEKD HDLLKRGDIV 180
  181 GVEGYVGRTQ PKKGGEGEVS VFVSRVQLLT PCLHMLPADH FGFKDQETRY RKRYLDLIMN 240
  241 KDARNRFITR SEIIRYIRRF LDQRKFIEVE TPMMNVIAGG ATAKPFITHH NDLDMDMYMR 300
  301 IAPELFLKQL VVGGLDRVYE IGRQFRNEGI DMTHNPEFTT CEFYQAYADV YDLMDMTELM 360
  361 FSEMVKEITG SYIIKYHPDP ADPAKELELN FSRPWKRINM IEELEKVFNV KFPSGDQLHT 420
  421 AETGEFLKKI LVDNKLECPP PLTNARMLDK LVGELEDTCI NPTFIFGHPQ MMSPLAKYSR 480
  481 DQPGLCERFE VFVATKEICN AYTELNDPFD QRARFEEQAR QKDQGDDEAQ LVDETFCNAL 540
  541 EYGLPPTGGW GCGIDRLAMF LTDSNTIREV LLFPTLKPDV LREEVKKEEE N

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2006)		KRS1 LSM1 LSM5 PAT1
View Details	Gavin AC, et al. (2002)		ASC1 CDC33 CDC48 CLF1 CWC23 DCP2 DHH1 DIB1 ERB1 KRS1 LSM1 LSM2 LSM3 LSM4 LSM5 LSM6 LSM7 NAM7 NOG2 NOP1 NUG1 PAT1 PRP24 PRP31 PRP38 PRP4 PRP43 PRP46 PRP6 SMB1 SMD2 SNU114 SNU23 SNU66 SPP382 SQS1 SRO9 TFB4
View Details	Ho Y, et al. (2002)		ACS2 ADE3 ADE6 ADO1 ALA1 APE2 ARA1 ARG1 BAT1 CDC10 CDC33 CLU1 COR1 CYS3 DED81 DPS1 ERG10 ERG20 FPR1 GCY1 GPH1 GRS1 HOM2 HYP2 ILS1 IMD3 KGD2 KRS1 LPD1 LSP1 MDH3 OLA1 PAB1 PFK2 PGM2 PMI40 PRP6 RVB2 RVS161 RVS167 SAC6 SCP160 SES1 SOD1 SSZ1 THR4 TRP5 TRR1 UBA1 YDL124W

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RBA50	No Comments	Hazbun TR, et al. (2003)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	UBP6	KRS1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..220]	PSI-BLAST	1121.0103	Lysyl-tRNA synthetase (LysRS)
2	View Details	[221..591]	PSI-BLAST	1121.0103	Lysyl-tRNA synthetase (LysRS)

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
ligase activity, forming aminoacyl-tRNA and related compounds	7.89468709683036	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.89468709683036	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.88410036819636	bayes_pls_golite062009
ligase activity	6.92073480193262	bayes_pls_golite062009
lysine-tRNA ligase activity	5.79033103181143	bayes_pls_golite062009
aspartate-tRNA ligase activity	3.80084837437429	bayes_pls_golite062009
phenylalanine-tRNA ligase activity	2.99105702052936	bayes_pls_golite062009
serine-tRNA ligase activity	2.46505535069103	bayes_pls_golite062009
structural constituent of ribosome	2.43759174701552	bayes_pls_golite062009
glycine-tRNA ligase activity	2.29229136935761	bayes_pls_golite062009
threonine-tRNA ligase activity	2.18642354927	bayes_pls_golite062009
structural molecule activity	2.08258913217268	bayes_pls_golite062009
catalytic activity	1.99372273395202	bayes_pls_golite062009
alanine-tRNA ligase activity	1.82594054955281	bayes_pls_golite062009
histidine-tRNA ligase activity	1.72215103383322	bayes_pls_golite062009
transcription regulator activity	1.67362693618722	bayes_pls_golite062009
binding	1.54403712698597	bayes_pls_golite062009
nucleic acid binding	1.48056625237411	bayes_pls_golite062009
DNA binding	1.44496421928708	bayes_pls_golite062009
translation initiation factor activity	0.96756670903925	bayes_pls_golite062009
asparagine-tRNA ligase activity	0.921492123574877	bayes_pls_golite062009
transcription factor activity	0.825973629967327	bayes_pls_golite062009
sequence-specific DNA binding	0.77755643935497	bayes_pls_golite062009
ribonuclease activity	0.702449673647117	bayes_pls_golite062009
nuclease activity	0.111225677144242	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2