Protein Overview: RPS8B, RPS8A

General Information:
Name(s) found:	RPS8B / YER102W [SGD] RPS8A / YBL072C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320949\|ref\|NP_011028.1\| Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein; Rps8bp [Saccharomyces cerevisiae] [NCBI NR] gi\|6319399\|ref\|NP_009481.1\| Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein; Rps8ap [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S45591 ribosomal protein S8.e, cytosolic - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|603340\|gb\|AAB64657.1\| Rps8bp: Ribosome protein, small subunit [Saccharomyces cerevisiae] [NCBI NR] gi\|536115\|emb\|CAA84893.1\| RPS8A [Saccharomyces cerevisiae] [NCBI NR] gi\|51013099\|gb\|AAT92843.1\| YER102W [Saccharomyces cerevisiae] [NCBI NR] gi\|464725\|sp\|P05754.3\|RS8_YEAST RecName: Full=40S ribosomal protein S8; AltName: Full=S14; AltName: Full=YS9; AltName: Full=RP19 [NCBI NR] gi\|407523\|emb\|CAA81525.1\| ribosomal protein S8 [Saccharomyces cerevisiae] [NCBI NR] 40S ribosomal protein S8 OS=Saccharomyces cerevisiae GN=RPS8A PE=1 SV=3 [Swiss-Prot] RPS8B Ribosomal protein S8.e [mips] RPS8A Ribosomal protein S8.e [mips] Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein [SGD] Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein [SGD] RPS8B, Chr V from 363096-363698 [yeast_orfs2] RPS8A, Chr II from 88518-89120, reverse complement [yeast_orfs2] Chr V from 363096-363698 [yeastorf3.fasta] Chr II from 88518-89120, reverse complement [yeastorf3.fasta] Chr II from 89120-88518, reverse complement [yeastorf5.fasta] RPS8B SGDID:S0000904, Chr V from 363096-363698, Verified ORF [yeastflysequest.fasta] RPS8A SGDID:S0000168, Chr II from 89123-88521, reverse complement, Verified ORF [yeastflysequest.fasta] Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein [Saccharomyces cerevisiae]gi\|6320949\|ref\|NP_011028.1\| Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp a [nrdb_11152004_Chlamydomonas] RPS8A SGDID:S000000168, Chr II from 89123-88521, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RPS8B SGDID:S000000904, Chr V from 363096-363698, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RPS8A SGDID:S000000168, Chr II from 89123-88521, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein" [yeast-contam.fasta] RPS8B SGDID:S000000904, Chr V from 363096-363698, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein" [yeast-contam.fasta] ribosomal protein S8 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YER102W [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Rps8bp: Ribosome protein, small subunit [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein; Rps8bp [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 40S ribosomal protein S8 (S14) (YS9) (RP19) [nr-271106-contam.fasta] Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein; Rps8ap [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RPS8A [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] RPS8A SGDID:S000000168, Chr II from 89125-88523, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein" [yeast-030211-contam.fasta] RPS8B SGDID:S000000904, Chr V from 363100-363702, Genome Release 64-1-1, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein" [yeast-030211-contam.fasta] RPS8A SGDID:S000000168, Chr II from 89125-88523, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication" [20141208_orf_trans.fasta] RPS8B SGDID:S000000904, Chr V from 363100-363702, Genome Release 64-2-1, Verified ORF, "Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8B has a paralog, RPS8A, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR998\|c0_g1_i1\|g.745 ORF TR998\|c0_g1_i1\|g.745 TR998\|c0_g1_i1\|m.745 type:complete len:201 (+) TR998\|c0_g1_i1:49-651(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR7340\|c0_g1_i1\|g.5378 ORF TR7340\|c0_g1_i1\|g.5378 TR7340\|c0_g1_i1\|m.5378 type:complete len:201 (+) TR7340\|c0_g1_i1:38-640(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YER102W SGDID:S000000904, Chr V from 363100-363702, Genome Release 64-1-1, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Ap and has similarity to rat S8 ribosomal protein" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] YBL072C SGDID:S000000168, Chr II from 89125-88523, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps8Bp and has similarity to rat S8 ribosomal protein" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_4895\|g.3314 ORF asmbl_4895\|g.3314 asmbl_4895\|m.3314 type:3prime_partial len:201 (+) asmbl_4895:32-631(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_4889\|g.3446 ORF asmbl_4889\|g.3446 asmbl_4889\|m.3446 type:3prime_partial len:201 (+) asmbl_4889:32-631(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_7051\|g.5222 ORF asmbl_7051\|g.5222 asmbl_7051\|m.5222 type:complete len:201 (+) asmbl_7051:396-998(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_4564\|g.2848 ORF ID=asmbl_4564\|g.2848 ID=asmbl_4564\|m.2848 type:3prime_partial len:201 (+) ID=asmbl_4564:339-938(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_7589\|g.4793 ORF ID=asmbl_7589\|g.4793 ID=asmbl_7589\|m.4793 type:complete len:201 (+) ID=asmbl_7589:378-980(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_4893\|g.2791 ORF ID=asmbl_4893\|g.2791 ID=asmbl_4893\|m.2791 type:3prime_partial len:201 (+) ID=asmbl_4893:339-938(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_7294\|g.4422 ORF ID=asmbl_7294\|g.4422 ID=asmbl_7294\|m.4422 type:complete len:201 (+) ID=asmbl_7294:396-998(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	200 amino acids
Gene Ontology:
Cellular Component:	ribonucleoprotein complex [IEA] cytosolic small ribosomal subunit [IDA] cytoplasm [IEA] 90S preribosome [IDA] ribosome [IEA] intracellular [IEA]
Biological Process:	translation [IDA]
Molecular Function:	structural constituent of ribosome [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI  60
   61 ETGNFSWASE GISKKTRIAG VVYHPSNNEL VRTNTLTKAA IVQIDATPFR QWFEAHYGQT 120
  121 LGKKKNVKEE ETVAKSKNAE RKWAARAASA KIESSVESQF SAGRLYACIS SRPGQSGRCD 180
  181 GYILEGEELA FYLRRLTAKK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ASC1 ECM16 FUN12 GBP2 HCA4 IMD4 KEM1 KRE33 KRI1 MAK5 NAT1 NHP6A NIP1 NOP14 NOP56 NPL3 PRP43 PRT1 PUF4 PUF6 RPG1 RPL10 RPL11B RPL12B, RPL12A RPL13B RPL15A RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL1A, RPL1B RPL20A RPL20B RPL21A RPL24A RPL2A, RPL2B RPL3 RPL30 RPL32 RPL36A RPL42B, RPL42A RPL4A RPL4B RPL5 RPL6A RPL8B RPL9A RPS11A, RPS11B RPS13 RPS14A RPS16B, RPS16A RPS17A RPS17B RPS1A RPS1B RPS2 RPS22A RPS22B RPS23B, RPS23A RPS24A, RPS24B RPS3 RPS30B, RPS30A RPS4B, RPS4A RPS5 RPS6A, RPS6B RPS7A RPS8B, RPS8A RPS9A RPS9B RRP12 SBP1 SRO9 STM1 TIF34 TOP2 TSR1 UTP20 UTP22 YGR054W
View Details	(MIPS) Mewes HW, et al. (2004)		ASC1 RPS0A RPS0B RPS10A RPS10B RPS11A, RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16B, RPS16A RPS17A RPS17B RPS18B, RPS18A RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B RPS23B, RPS23A RPS24A, RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30B, RPS30A RPS31 RPS4B, RPS4A RPS5 RPS6A, RPS6B RPS7A RPS7B RPS8B, RPS8A RPS9A RPS9B
View Details	Qiu et al. (2008)		APT1 ASC1 ATG27 BUD20 EFT2, EFT1 HMS2 MNL1 NAT3 PUF2 RKI1 RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL14B RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL20B RPL21A RPL21B RPL22A RPL22B RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27A RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL34B RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL4B RPL5 RPL6A RPL6B RPL7A RPL7B RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B RPS0A RPS0B RPS10A RPS10B RPS11A, RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16B, RPS16A RPS17A RPS17B RPS18B, RPS18A RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B RPS23B, RPS23A RPS24A, RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30B, RPS30A RPS31 RPS4B, RPS4A RPS5 RPS6A, RPS6B RPS7A RPS7B RPS8B, RPS8A RPS9A RPS9B SFT1 SOP4 TEF1, TEF2 TOS4 TSR1 VAR1 YML6 YPL199C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	MIH1	control 2	McCusker D, et al (2007)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	ASF1	sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant)	Green EM, et al (2005)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CDC48	RPS8B, RPS8A	1	Unpublished Fields Lab Data
View Screen	SPT21	RPS8B, RPS8A	1	Unpublished Fields Lab Data
View Screen	RPB2	RPS8B, RPS8A	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	RPS8B, RPS8A	Huh WK, et al. (2003)
View Data	RPS8B, RPS8A	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..128]	Pfam	83.60206	Ribosomal protein S8e No confident structure predictions are available.
2	View Details	[129..200]	MSA	4.040976	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2