Protein Overview: PFS2

General Information:
Name(s) found:	PFS2 / YNL317W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|633666\|emb\|CAA86378.1\| NO348 [Saccharomyces cerevisiae] [NCBI NR] gi\|6324012\|ref\|NP_014082.1\| Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing [NCBI NR] gi\|1302427\|emb\|CAA96247.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1176585\|sp\|P42841.1\|PFS2_YEAST RecName: Full=Polyadenylation factor subunit 2 [NCBI NR] pir\|\|S51295 hypothetical protein YNL317w - yeast (Saccharomyces cerevisiae) [NCBI NR] (P42841) Hypothetical 53.1 kDa Trp-Asp repeats containing protein in HXT14-PHA2 intergenic region [Swiss-Prot] Polyadenylation factor subunit 2 OS=Saccharomyces cerevisiae GN=PFS2 PE=1 SV=1 [Swiss-Prot] PFS2 Polyadenylation factor I subunit 2 required for mRNA 3`-end processing, bridges two mRNA 3`-end processing factors [mips] Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex [SGD] Chr XIV from 40618-42015 [yeastorf5.fasta] PFS2, Chr XIV from 40618-42015 [yeast_orfs2] PFS2 SGDID:S0005261, Chr XIV from 40618-42015, Verified ORF [yeastflysequest.fasta] Intergral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex [Saccharomyces cere [nrdb_11152004_Chlamydomonas] PFS2 SGDID:S000005261, Chr XIV from 40618-42015, Verified ORF, "Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promo [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NO348 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex; Pfs2p [Saccharomyce [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Polyadenylation factor subunit 2 [nr-271106-contam.fasta] PFS2 SGDID:S000005261, Chr XIV from 40618-42015, Verified ORF, "Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Polyadenylation factor subunit 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PFS2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PFS2 SGDID:S000005261, Chr XIV from 40619-42016, Genome Release 64-1-1, Verified ORF, "Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex" [yeast-030211-contam.fasta] PFS2 SGDID:S000005261, Chr XIV from 40619-42016, Genome Release 64-2-1, Verified ORF, "Integral subunit of the pre-mRNA CPF complex; the cleavage and polyadenylation factor (CPF) complex plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex" [20141208_orf_trans.fasta] YNL317W SGDID:S000005261, Chr XIV from 40619-42016, Genome Release 64-1-1, Verified ORF, "Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_5477\|g.3977 ORF asmbl_5477\|g.3977 asmbl_5477\|m.3977 type:complete len:466 (+) asmbl_5477:53-1450(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_5474\|g.3615 ORF ID=asmbl_5474\|g.3615 ID=asmbl_5474\|m.3615 type:complete len:466 (+) ID=asmbl_5474:53-1450(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	465 amino acids
Gene Ontology:
Cellular Component:	mRNA cleavage and polyadenylation specificity factor complex [IPI][TAS]
Biological Process:	mRNA polyadenylation [IDA][IMP][TAS] mRNA cleavage [IDA][IMP][TAS]
Molecular Function:	RNA binding [IC]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDGHNQNQYQ NQNQIQQSQQ PPLKKYVTQR RSVDVSSPYI NLYYNRRHGL PNLVVEPETS  60
   61 YTIDIMPPNA YRGRDRVINL PSKFTHLSSN KVKHVIPAIQ WTPEGRRLVV ATYSGEFSLW 120
  121 NASSFTFETL MQAHDSAVTT MKYSHDSDWM ISGDADGMIK IWQPNFSMVK EIDAAHTESI 180
  181 RDMAFSSNDS KFVTCSDDNI LKIWNFSNGK QERVLSGHHW DVKSCDWHPE MGLIASASKD 240
  241 NLVKLWDPRS GNCISSILKF KHTVLKTRFQ PTKGNLLMAI SKDKSCRVFD IRYSMKELMC 300
  301 VRDETDYMTL EWHPINESMF TLACYDGSLK HFDLLQNLNE PILTIPYAHD KCITSLSYNP 360
  361 VGHIFATAAK DRTIRFWTRA RPIDPNAYDD PTYNNKKING WFFGINNDIN AVREKSEFGA 420
  421 APPPPATLEP HALPNMNGFI NKKPRQEIPG IDSNIKSSTL PGLSI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CFT1 CFT2 FIP1 GLC7 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 RNA14 SSU72 SWD2 YSH1 YTH1
View Details	Krogan NJ, et al. (2006)		AIM1 CFT1 CFT2 ECM21 FIP1 GIP4 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 SSU72 SWD2 SYC1 YBL010C YLR358C YSH1 YTH1
View Details	Gavin AC, et al. (2006)		CFT1 CFT2 FIP1 MPE1 PAP1 PFS2 PTA1 YSH1 YTH1
View Details	Gavin AC, et al. (2002)		ACT1 ADE4 AIM31 BFR2 BMS1 BUD21 CBF5 CFT1 CFT2 CKA1 CLP1 DBP8 DIP2 ECM16 ECM29 EMG1 ENO2 ENP1 ENP2 ERO1 ESF1 ESF2 FIP1 GCN1 GLC7 HAS1 HCA4 HHF1, HHF2 HSL1 HTA1 HTA2 IMP3 IMP4 KRE33 KRR1 MPE1 MPP10 MVD1 NAN1 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PCF11 PFK1 PFS2 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 REF2 RML2 RNA14 RNA15 ROK1 RPP2B RRP9 RSA3 RTG2 SCL1 SEC13 SEC21 SEC31 SOF1 SRO9 SSA3 SSU72 SYC1 TAF6 TIF1, TIF2 TIF4632 TYE7 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 VID24 VMR1 VPS53 YCL046W YSH1 YTH1
View Details	Ho Y, et al. (2002)		CCT2 CCT5 CCT6 CFT1 HGH1 PFS2 TCP1
View Details	Qiu et al. (2008)		CFT1 CFT2 CLP1 FIP1 HRP1 PAP1 PFS2 PTA1 PTI1 REF2 RNA14 RNA15 YSH1 YTH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	PFS2	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..333]	PSI-BLAST	224.69897	beta1-subunit of the signal-transducing G protein heterotrimer
2	View Details	[334..465]	ORFEUS	13.83	No description for 1scgB was found.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: PFS2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: