Protein Overview: CWC22

General Information:
Name(s) found:	CWC22 / YGR278W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321717\|ref\|NP_011794.1\| Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-protein interaction data; Cwc22p [Saccharomy [NCBI NR] pir\|\|S64613 probable membrane protein YGR278w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1723782\|sp\|P53333.1\|CWC22_YEAST RecName: Full=Pre-mRNA-splicing factor CWC22; AltName: Full=Complexed with CEF1 protein 22 [NCBI NR] gi\|1323507\|emb\|CAA97309.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] (P53333) Hypothetical 67.3 kDa protein in TAF145-YOR1 intergenic region [Swiss-Prot] Pre-mRNA-splicing factor CWC22 OS=Saccharomyces cerevisiae GN=CWC22 PE=1 SV=1 [Swiss-Prot] CWC22 Protein involved in mRNA splicing [mips] Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-protein interaction data [SGD] YGR278W, Chr VII from 1046728-1048461 [yeast_orfs2] Chr VII from 1046728-1048461 [yeastorf3.fasta] Chr VII from 1046735-1048468 [yeastorf4.fasta] CWC22 SGDID:S0003510, Chr VII from 1046736-1048469, Verified ORF [yeastflysequest.fasta] CWC22 SGDID:S0003510, Chr VII from 1046737-1048470, Verified ORF [10112005cervSGD.fasta] Cwc22p [Saccharomyces cerevisiae]gi\|1323507\|emb\|CAA97309.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|2132635\|pir\|\|S64613 probable membrane protein YGR278w - yeast (Saccharomyces cerevisiae)gi\|1723782\|sp\|P53333\|YG5U_YEAST HYPOTHETICAL 67.3 K [nrdb_11152004_Chlamydomonas] CWC22 SGDID:S000003510, Chr VII from 1046737-1048470, Verified ORF, "Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-pro [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] CWC22 SGDID:S000003510, Chr VII from 1046738-1048471, Verified ORF, "Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-pro [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-protein interaction data; Cwc22p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Pre-mRNA-splicing factor CWC22 (Complexed with CEF1 protein 22) [nr-271106-contam.fasta] CWC22 SGDID:S000003510, Chr VII from 1046738-1048471, Verified ORF, "Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-protein interaction data" [yeast-bovrabprots.fasta] CWC22 SGDID:S000003510, Chr VII from 1046737-1048470, Verified ORF, "Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-protein interaction data" [yeast-contam_custom.fasta] [chemostatdb.fasta] Pre-mRNA-splicing factor CWC22 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CWC22 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CWC22 SGDID:S000003510, Chr VII from 1046731-1048464, Genome Release 64-1-1, Verified ORF, "Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein" [yeast-030211-contam.fasta] CWC22 SGDID:S000003510, Chr VII from 1046731-1048464, Genome Release 64-2-1, Verified ORF, "Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein" [20141208_orf_trans.fasta] YGR278W SGDID:S000003510, Chr VII from 1046731-1048464, Genome Release 64-1-1, Verified ORF, "Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	577 amino acids
Gene Ontology:
Cellular Component:	spliceosomal complex [IPI]
Biological Process:	nuclear mRNA splicing, via spliceosome [IPI]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL IGRNIICKNV  60
   61 VDFTLNKQNG RLIPALSALI ALLNSDIPDI GETLAKELML MFVQQFNRKD YVSCGNILQC 120
  121 LSILFLYDVI HEIVILQILL LLLEKNSLRL VIAVMKICGW KLALVSKKTH DMIWEKLRYI 180
  181 LQTQELSSTL RESLETLFEI RQKDYKSGSQ GLFILDPTSY TVHTHSYIVS DEDEANKELG 240
  241 NFEKCENFNE LTMAFDTLRQ KLLINNTSDT NEGSNSQLQI YDMTSTNDVE FKKKIYLVLK 300
  301 SSLSGDEAAH KLLKLKIANN LKKSVVDIII KSSLQESTFS KFYSILSERM ITFHRSWQTA 360
  361 YNETFEQNYT QDIEDYETDQ LRILGKFWGH LISYEFLPMD CLKIIKLTEE ESCPQGRIFI 420
  421 KFLFQELVNE LGLDELQLRL NSSKLDGMFP LEGDAEHIRY SINFFTAIGL GLLTEDMRSR 480
  481 LTIIQEVEDA EEEEKKLREE EELEKLRKKA RESQPTQGPK IHESRLFLQK DTRENSRSRS 540
  541 PFTVETRKRA RSRTPPRGSR NHRNRSRTPP ARRQRHR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BRR2 BUD31 CDC40 CEF1 CLF1 CWC2 CWC22 CWC23 HSH155 PRP19 PRP43 PRP45 PRP46 PRP8 RSE1 SNT309 SNU114 SPP382 SYF1
View Details	Hazbun TR, et al. (2003)		BRR2 CDC40 CEF1 CLF1 CWC2 CWC22 CWC23 ECM2 ISY1 LEA1 NTR2 PRP19 PRP43 PRP45 PRP46 PRP8 SMB1 SMD1 SMD3 SNT309 SNU114 SPP382 SYF1 SYF2 YJU2
View Details	(MIPS) Mewes HW, et al. (2004)		AAR2 BRR1 BRR2 CBC2 CDC40 CWC15 CWC2 CWC22 CWC23 DHR2 LSM4 MAK5 MUD2 PRP16 PRP18 PRP2 PRP22 PRP24 PRP28 PRP31 PRP38 PRP39 PRP4 PRP40 PRP43 PRP5 PRP6 PRP8 SLU7 SMD1 SMD3 SME1 SNP1 SPP2 SPP381 STO1 YHC1
View Details	Krogan NJ, et al. (2006)		CWC22 MSS18 PRP19 PRP46 YLR132C
View Details	Gavin AC, et al. (2006)		CDC40 CEF1 CLF1 CWC2 CWC22 ECM2 PRP19 PRP45 PRP46 SYF1
View Details	Gavin AC, et al. (2002)		AAR2 BRR1 BUD31 CDC33 CDC40 CEF1 CLF1 CSN12 CUS1 CWC2 CWC22 CWC23 DHH1 DIB1 ECM2 GCN2 HSH155 HTA1 IMG1 ISY1 LEA1 LIN1 LSM1 LSM2 LSM4 LSM5 LSM6 LSM7 LUC7 MRP7 MRPL28 MRPL3 MRPL35 MRPL38 MRPL4 MRPL8 MRPS5 MSL1 MUD1 PAT1 PRP11 PRP18 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP46 PRP6 PRP8 PRP9 RSE1 RVB2 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP381 SPP382 SRB2 STO1 SYF1 TOS4 UTP21 YML6
View Details	Qiu et al. (2008)		AAR2 BRR1 BRR2 BUD31 CBC2 CDC40 CEF1 CLF1 CUS1 CWC15 CWC2 CWC21 CWC22 CWC23 CWC25 DHR2 DIB1 ECM2 HSH155 HSH49 ISY1 LEA1 LSM2 LSM3 LSM4 LSM7 LUC7 MAK5 MSL1 MUD1 MUD2 NAM8 NTC20 PRP11 PRP16 PRP18 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP46 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP2 SPP381 SPP382 STO1 SUB2 SYF1 SYF2 YHC1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CWC2	No Comments	Hazbun TR, et al. (2003)
View Run	CWC25	No Comments	Hazbun TR, et al. (2003)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CTF13	CWC22	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CWC22 and SPC42	Unpublished Data
View Data	CWC22	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..226]	PSI-BLAST	45.0	Eukaryotic initiation factor eIF4G
2	View Details	[227..300]	deduced	N/A	No confident structure predictions are available.
3	View Details	[301..514]	ORFEUS	7.46	CBP80, 80KDa nuclear cap-binding protein
4	View Details	[515..577]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
RNA binding	4.20683443264388	bayes_pls_golite062009
mRNA binding	2.49094746544717	bayes_pls_golite062009
binding	2.47395644967726	bayes_pls_golite062009
nucleic acid binding	1.64539435934931	bayes_pls_golite062009
structural molecule activity	1.39201458088376	bayes_pls_golite062009
transcription regulator activity	1.06250847754412	bayes_pls_golite062009
protein binding	1.04044934052933	bayes_pls_golite062009
DNA binding	0.946847037737779	bayes_pls_golite062009
RNA cap binding	0.66277586901495	bayes_pls_golite062009
transcription factor activity	0.362911773502049	bayes_pls_golite062009

Term	Confidence	Notes
RNA binding	4.05046860305563	bayes_pls_golite062009
binding	2.36800795249393	bayes_pls_golite062009
mRNA binding	2.35042158577052	bayes_pls_golite062009
nucleic acid binding	1.59410102249793	bayes_pls_golite062009
structural molecule activity	1.3916221897201	bayes_pls_golite062009
transcription regulator activity	1.08645493064764	bayes_pls_golite062009
DNA binding	0.956143892328242	bayes_pls_golite062009
protein binding	0.914877292844892	bayes_pls_golite062009
transcription factor activity	0.371244009721798	bayes_pls_golite062009
RNA cap binding	0.357520253345298	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.87

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2